(Linear) (Six-base) MAPSORT of: KIEE6510.seq  Check: 6080  from: 1  to: 234



KIEE6510.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:32  ..

AccI GT'mk_AC

Cuts at:      0      75     234 
   Size:         75     159
 
AvaI C'yCGr_G

Cuts at:      0      50     234 
   Size:         50     184
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      90     111     234 
   Size:         90      21     123

  Fragments arranged by size: 

                 123      90      21
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      50     234 
   Size:         50     184
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      47      77     234 
   Size:         47      30     157

  Fragments arranged by size: 

                 157      47      30
 
BsrGI T'GTAC_A

Cuts at:      0      80     234 
   Size:         80     154
 
BssSI C'ACGA_G

Cuts at:      0     119     234 
   Size:        119     115
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      90     111     234 
   Size:         90      21     123

  Fragments arranged by size: 

                 123      90      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     196     234 
   Size:        196      38
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     164     234 
   Size:        164      70
 
PfoI T'CCnGG_A

Cuts at:      0     109     213     234 
   Size:        109     104      21

  Fragments arranged by size: 

                 109     104      21
 
PpuMI rG'GwC_Cy

Cuts at:      0     196     234 
   Size:        196      38
 
PshAI GACnn'nnGTC

Cuts at:      0      92     234 
   Size:         92     142
 
SanDI GG'GwC_CC

Cuts at:      0     196     234 
   Size:        196      38
 
SexAI A'CCwGG_T

Cuts at:      0      59     234 
   Size:         59     175
 
SfcI C'TryA_G

Cuts at:      0      11     234 
   Size:         11     223
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     164     234 
   Size:        164      70
 
TatI w'GTAC_w

Cuts at:      0      80     234 
   Size:         80     154
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      47      79     234 
   Size:         47      32     155

  Fragments arranged by size: 

                 155      47      32
 

 Enzymes that do cut:

     AccI     AvaI     BpmI     BsaI    BsaXI    BsrGI    BssSI  Eco57MI
 EcoO109I     MmeI     PfoI    PpuMI    PshAI    SanDI    SexAI     SfcI
    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI