(Circular) (Six-base) MAPSORT of: pFN21AE5777.seq Check: 4760 from: 1 to: 6601 pFN21AE5777 6601 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3260 4411 278 Size: 53 83 186 936 1724 1151 2468 Fragments arranged by size: 2468 1724 1151 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2820 3045 3825 3888 3944 1937 Size: 883 225 780 63 56 4594 Fragments arranged by size: 4594 883 780 225 63 56 Acc65I G'GTAC_C Cuts at: 3922 3922 Size: 6601 AclI AA'CG_TT Cuts at: 1563 4728 5101 1563 Size: 3165 373 3063 Fragments arranged by size: 3165 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2795 3798 4664 6048 6316 1632 Size: 1163 1003 866 1384 268 1917 Fragments arranged by size: 1917 1384 1163 1003 866 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6404 AflIII A'CryG_T Cuts at: 1203 5500 6477 1203 Size: 4297 977 1327 Fragments arranged by size: 4297 1327 977 AgeI A'CCGG_T Cuts at: 5432 5432 Size: 6601 AhdI GACnn_n'nnGTC Cuts at: 3832 5330 3832 Size: 1498 5103 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6601 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6601 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2864 2896 2864 Size: 32 6569 AlwNI CAG_nnn'CTG Cuts at: 2058 3675 3737 4344 5916 2058 Size: 1617 62 607 1572 2743 Fragments arranged by size: 2743 1617 1572 607 62 ApaLI G'TGCA_C Cuts at: 4658 5814 4658 Size: 1156 5445 ApoI r'AATT_y Cuts at: 1493 2096 2233 2570 3910 4136 4288 6397 1493 Size: 603 137 337 1340 226 152 2109 1697 Fragments arranged by size: 2109 1697 1340 603 337 226 152 137 AseI AT'TA_AT Cuts at: 160 1032 5155 160 Size: 872 4123 1606 Fragments arranged by size: 4123 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6601 AvaI C'yCGr_G Cuts at: 1870 1943 3926 1870 Size: 73 1983 4545 Fragments arranged by size: 4545 1983 73 AvrII C'CTAG_G Cuts at: 3089 3089 Size: 6601 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2762 2795 2762 Size: 33 6568 BamHI G'GATC_C Cuts at: 3931 4298 3931 Size: 367 6234 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2243 3081 3922 5377 618 Size: 325 217 627 456 838 841 1455 1842 Fragments arranged by size: 1842 1455 841 838 627 456 325 217 BanII G_rGCy'C Cuts at: 1391 3521 3699 3920 1391 Size: 2130 178 221 4072 Fragments arranged by size: 4072 2130 221 178 BbeI G_GCGC'C Cuts at: 3085 3085 Size: 6601 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2637 3189 928 Size: 971 738 552 4340 Fragments arranged by size: 4340 971 738 552 BbvCI CC'TCA_GC Cuts at: 3299 3299 Size: 6601 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3442 3476 3617 3651 4792 4826 1903 Size: 34 1505 34 141 34 1141 34 3678 Fragments arranged by size: 3678 1505 1141 141 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4493 5703 4493 Size: 1210 5391 BclI T'GATC_A Cuts at: 1548 1548 Size: 6601 BfrBI ATG'CAT Cuts at: 2407 2407 Size: 6601 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3227 3281 3335 Size: 107 122 71 631 605 1555 54 54 Cuts at: 3335 5212 136 Size: 1877 1525 Fragments arranged by size: 1877 1555 1525 631 605 122 107 71 54 54 BglII A'GATC_T Cuts at: 2292 6596 2292 Size: 4304 2297 BlpI GC'TnA_GC Cuts at: 2359 4018 2359 Size: 1659 4942 Bme1580I G_kGCm'C Cuts at: 1790 2127 2248 4662 5818 1790 Size: 337 121 2414 1156 2573 Fragments arranged by size: 2573 2414 1156 337 121 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6601 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2076 3540 3892 Size: 416 53 83 399 24 659 1464 352 Cuts at: 3892 4332 5290 442 Size: 440 958 1753 Fragments arranged by size: 1753 1464 958 659 440 416 399 352 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6601 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2131 2131 Size: 6601 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3056 3363 5261 1239 Size: 1817 307 1898 2579 Fragments arranged by size: 2579 1898 1817 307 Bpu10I CC'TnA_GC Cuts at: 3299 3353 3299 Size: 54 6547 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3535 4726 5591 5889 3535 Size: 1191 865 298 4247 Fragments arranged by size: 4247 1191 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2221 2366 3097 5264 882 Size: 617 722 145 731 2167 2219 Fragments arranged by size: 2219 2167 731 722 617 145 BsaAI yAC'GTr Cuts at: 493 1204 4392 493 Size: 711 3188 2702 Fragments arranged by size: 3188 2702 711 BsaBI GATnn'nnATC Cuts at: 6595 6595 Size: 6601 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3082 3257 4408 4790 Size: 53 83 186 936 1549 175 1151 382 Cuts at: 4790 5425 275 Size: 635 1451 Fragments arranged by size: 1549 1451 1151 936 635 382 186 175 83 53 BsaWI w'CCGG_w Cuts at: 5034 5432 5706 5853 6264 5034 Size: 398 274 147 411 5371 Fragments arranged by size: 5371 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6376 Fragments arranged by size: 6376 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2104 2696 1186 Size: 918 592 5091 Fragments arranged by size: 5091 918 592 BseYI C'CCAG_C Cuts at: 3211 3276 3597 5804 6300 3211 Size: 65 321 2207 496 3512 Fragments arranged by size: 3512 2207 496 321 65 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3179 1163 Size: 573 1443 4585 Fragments arranged by size: 4585 1443 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4051 4812 5840 1540 Size: 275 186 2050 761 1028 2301 Fragments arranged by size: 2301 2050 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3521 3699 3770 4662 4747 5818 3521 Size: 178 71 892 85 1071 4304 Fragments arranged by size: 4304 1071 892 178 85 71 BsmI GAATG_Cn' Cuts at: 4112 4205 4112 Size: 93 6508 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2127 2248 3521 3699 3770 3920 4662 Size: 399 337 121 1273 178 71 150 742 Cuts at: 4662 4747 5818 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1273 1071 742 399 337 178 150 121 85 71 BspEI T'CCGG_A Cuts at: 6264 6264 Size: 6601 BspHI T'CATG_A Cuts at: 4490 6220 4490 Size: 1730 4871 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3956 844 Size: 3112 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4488 1394 Size: 429 2665 3507 Fragments arranged by size: 3507 2665 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3117 5096 5270 65 Size: 1183 1869 1979 174 1396 Fragments arranged by size: 1979 1869 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5245 5432 1700 Size: 110 3435 187 2869 Fragments arranged by size: 3435 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6601 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6601 BssSI C'ACGA_G Cuts at: 2278 3002 4661 5673 2278 Size: 724 1659 1012 3206 Fragments arranged by size: 3206 1659 1012 724 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6601 BstBI TT'CG_AA Cuts at: 5405 5405 Size: 6601 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6601 BstXI CCAn_nnnn'nTGG Cuts at: 3476 3539 3476 Size: 63 6538 BstYI r'GATC_y Cuts at: 1972 2292 2560 3931 4298 4683 4700 6152 6238 Size: 320 268 1371 367 385 17 1452 86 Cuts at: 6238 6250 6596 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1371 385 367 346 320 268 86 17 12 BstZ17I GTA'TAC Cuts at: 3889 3889 Size: 6601 BtgI C'CryG_G Cuts at: 513 1065 2004 3469 3475 3532 3538 3568 3706 Size: 552 939 1465 6 57 6 30 138 Cuts at: 3706 513 Size: 3408 Fragments arranged by size: 3408 1465 939 552 138 57 30 6 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5862 Fragments arranged by size: 5862 683 56 BtsI GCAGTG_nn' Cuts at: 2040 4117 4911 4931 2040 Size: 2077 794 20 3710 Fragments arranged by size: 3710 2077 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6601 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6566 DraI TTT'AAA Cuts at: 3905 3905 Size: 6601 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5608 809 Size: 507 4292 1802 Fragments arranged by size: 4292 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3282 3336 3472 Size: 54 1425 50 275 18 1452 54 136 Cuts at: 3472 3535 3709 4048 4937 6566 8 Size: 63 174 339 889 1629 43 Fragments arranged by size: 1629 1452 1425 889 339 275 174 136 63 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4048 1537 Size: 275 2236 4090 Fragments arranged by size: 4090 2236 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2150 3106 3851 4531 1353 Size: 797 956 745 680 3423 Fragments arranged by size: 3423 956 797 745 680 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2210 5188 5562 5708 387 Size: 1064 138 621 2978 374 146 1280 Fragments arranged by size: 2978 1280 1064 621 374 146 138 EcoICRI GAG'CTC Cuts at: 3519 3697 3519 Size: 178 6423 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2795 3056 3363 3798 4664 5261 6048 Size: 393 1163 261 307 435 866 597 787 Cuts at: 6048 6316 1239 Size: 268 1524 Fragments arranged by size: 1524 1163 866 787 597 435 393 307 268 261 EcoO109I rG'GnC_Cy Cuts at: 2055 2422 3074 3220 3328 3601 3679 2055 Size: 367 652 146 108 273 78 4977 Fragments arranged by size: 4977 652 367 273 146 108 78 EcoRI G'AATT_C Cuts at: 3910 4288 3910 Size: 378 6223 EcoRV GAT'ATC Cuts at: 2859 2992 2859 Size: 133 6468 FspI TGC'GCA Cuts at: 5107 5107 Size: 6601 HaeII r_GCGC'y Cuts at: 1678 3085 3532 5748 1678 Size: 1407 447 2216 2531 Fragments arranged by size: 2531 2216 1407 447 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2202 2234 3350 3382 3713 Size: 32 135 32 630 32 1116 32 331 Cuts at: 3713 3745 3818 3850 5242 5274 5316 5348 1373 Size: 32 73 32 1392 32 42 32 2626 Fragments arranged by size: 2626 1392 1116 630 331 135 73 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2800 3826 3945 4191 669 Size: 988 281 862 1026 119 246 3079 Fragments arranged by size: 3079 1026 988 862 281 246 119 HindIII A'AGCT_T Cuts at: 748 748 Size: 6601 HpaI GTT'AAC Cuts at: 2800 4191 2800 Size: 1391 5210 KasI G'GCGC_C Cuts at: 3081 3081 Size: 6601 KpnI G_GTAC'C Cuts at: 3926 3926 Size: 6601 MfeI C'AATT_G Cuts at: 2901 4200 2901 Size: 1299 5302 MluI A'CGCG_T Cuts at: 6477 6477 Size: 6601 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2554 5715 5899 1305 Size: 1249 3161 184 2007 Fragments arranged by size: 3161 2007 1249 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3284 3338 3474 3537 3711 Size: 54 1425 343 1452 54 136 63 174 Cuts at: 3711 10 Size: 2900 Fragments arranged by size: 2900 1452 1425 343 174 136 63 54 54 MslI CAynn'nnrTG Cuts at: 518 1166 2505 2672 3393 3474 3492 3537 3897 Size: 648 1339 167 721 81 18 45 360 Cuts at: 3897 4559 4918 5077 518 Size: 662 359 159 2042 Fragments arranged by size: 2042 1339 721 662 648 360 359 167 159 81 45 18 MspA1I CmG'CkG Cuts at: 1606 3280 4017 4694 5842 6087 1606 Size: 1674 737 677 1148 245 2120 Fragments arranged by size: 2120 1674 1148 737 677 245 NarI GG'CG_CC Cuts at: 3082 3082 Size: 6601 NcoI C'CATG_G Cuts at: 513 1065 2004 3469 3475 3532 3538 3568 3706 Size: 552 939 1465 6 57 6 30 138 Cuts at: 3706 513 Size: 3408 Fragments arranged by size: 3408 1465 939 552 138 57 30 6 6 NdeI CA'TA_TG Cuts at: 387 3894 387 Size: 3507 3094 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6601 NotI GC'GGCC_GC Cuts at: 4048 4048 Size: 6601 NsiI A_TGCA'T Cuts at: 2409 2409 Size: 6601 NspI r_CATG'y Cuts at: 1132 1229 2023 3959 5504 1132 Size: 97 794 1936 1545 2229 Fragments arranged by size: 2229 1936 1545 794 97 PasI CC'CwG_GG Cuts at: 1348 3761 1348 Size: 2413 4188 PciI A'CATG_T Cuts at: 5500 5500 Size: 6601 PflMI CCAn_nnn'nTGG Cuts at: 1108 3142 3761 1108 Size: 2034 619 3948 Fragments arranged by size: 3948 2034 619 PmeI GTTT'AAAC Cuts at: 3905 3905 Size: 6601 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4670 4702 6183 6215 4670 Size: 32 1481 32 5056 Fragments arranged by size: 5056 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2055 2422 3074 3601 2055 Size: 367 652 527 5055 Fragments arranged by size: 5055 652 527 367 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6601 PsiI TTA'TAA Cuts at: 4171 4171 Size: 6601 PstI C_TGCA'G Cuts at: 830 2890 3953 830 Size: 2060 1063 3478 Fragments arranged by size: 3478 2060 1063 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6601 PvuII CAG'CTG Cuts at: 3280 3280 Size: 6601 SacI G_AGCT'C Cuts at: 3521 3699 3521 Size: 178 6423 SalI G'TCGA_C Cuts at: 1936 3824 3943 1936 Size: 1888 119 4594 Fragments arranged by size: 4594 1888 119 SanDI GG'GwC_CC Cuts at: 2422 2422 Size: 6601 SbfI CC_TGCA'GG Cuts at: 3953 3953 Size: 6601 ScaI AGT'ACT Cuts at: 4849 6561 4849 Size: 1712 4889 SexAI A'CCwGG_T Cuts at: 2598 2598 Size: 6601 SfcI C'TryA_G Cuts at: 826 1045 2541 2886 3064 3949 5084 5765 5956 Size: 219 1496 345 178 885 1135 681 191 Cuts at: 5956 826 Size: 1471 Fragments arranged by size: 1496 1471 1135 885 681 345 219 191 178 SfiI GGCCn_nnn'nGGCC Cuts at: 3281 3335 3281 Size: 54 6547 SfoI GGC'GCC Cuts at: 3083 3083 Size: 6601 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6601 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6601 SmaI CCC'GGG Cuts at: 1872 3928 1872 Size: 2056 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3514 4705 5606 5868 820 Size: 197 926 1571 1191 901 262 1553 Fragments arranged by size: 1571 1553 1191 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4392 493 Size: 3899 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6601 SphI G_CATG'C Cuts at: 1132 2023 3959 1132 Size: 891 1936 3774 Fragments arranged by size: 3774 1936 891 SspI AAT'ATT Cuts at: 5 52 4525 5 Size: 47 4473 2081 Fragments arranged by size: 4473 2081 47 StuI AGG'CCT Cuts at: 1517 2591 1517 Size: 1074 5527 StyI C'CwwG_G Cuts at: 513 1065 2004 3089 3469 3475 3532 3538 3568 Size: 552 939 1085 380 6 57 6 30 Cuts at: 3568 3706 4040 513 Size: 138 334 3074 Fragments arranged by size: 3074 1085 939 552 380 334 138 57 30 6 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2245 2517 3753 4628 4645 4798 4983 Size: 1255 665 272 1236 875 17 153 185 Cuts at: 4983 5428 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1236 875 665 445 272 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4847 6559 96 Size: 274 80 33 51 1443 2870 1712 138 Fragments arranged by size: 2870 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6561 6593 6561 Size: 32 6569 Tth111I GACn'n_nGTC Cuts at: 1316 3188 3812 1316 Size: 1872 624 4105 Fragments arranged by size: 4105 1872 624 XbaI T'CTAG_A Cuts at: 3937 3937 Size: 6601 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3545 3545 Size: 6601 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6601 XmaI C'CCGG_G Cuts at: 1870 3926 1870 Size: 2056 4545 XmnI GAAnn'nnTTC Cuts at: 2144 4730 2144 Size: 2586 4015 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3258 4409 276 Size: 53 83 186 936 1724 1151 2468 Fragments arranged by size: 2468 1724 1151 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BsiWI BsmBI Bsu36I DraIII EcoNI FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SrfI SwaI