(Linear) (Six-base) MAPSORT of: KIEE5777.seq Check: 4689 from: 1 to: 1896 KIEE5777.orf With 182 enzymes: SgfI * December 5, 2014 13:10 .. AatII G_ACGT'C Cuts at: 0 1255 1896 Size: 1255 641 AccI GT'mk_AC Cuts at: 0 815 1040 1820 1883 1896 Size: 815 225 780 63 13 Fragments arranged by size: 815 780 225 63 13 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 790 1793 1896 Size: 790 1003 103 Fragments arranged by size: 1003 790 103 AhdI GACnn_n'nnGTC Cuts at: 0 1827 1896 Size: 1827 69 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 859 891 1896 Size: 859 32 1005 Fragments arranged by size: 1005 859 32 AlwNI CAG_nnn'CTG Cuts at: 0 53 1670 1732 1896 Size: 53 1617 62 164 Fragments arranged by size: 1617 164 62 53 ApoI r'AATT_y Cuts at: 0 91 228 565 1896 Size: 91 137 337 1331 Fragments arranged by size: 1331 337 137 91 AvrII C'CTAG_G Cuts at: 0 1084 1896 Size: 1084 812 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 757 790 1896 Size: 757 33 1106 Fragments arranged by size: 1106 757 33 BanI G'GyrC_C Cuts at: 0 238 1076 1896 Size: 238 838 820 Fragments arranged by size: 838 820 238 BanII G_rGCy'C Cuts at: 0 1516 1694 1896 Size: 1516 178 202 Fragments arranged by size: 1516 202 178 BbeI G_GCGC'C Cuts at: 0 1080 1896 Size: 1080 816 BbsI GAAGACnn'nnnn_ Cuts at: 0 632 1184 1896 Size: 632 552 712 Fragments arranged by size: 712 632 552 BbvCI CC'TCA_GC Cuts at: 0 1294 1896 Size: 1294 602 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1437 1471 1612 1646 1896 Size: 1437 34 141 34 250 Fragments arranged by size: 1437 250 141 34 34 BfrBI ATG'CAT Cuts at: 0 402 1896 Size: 402 1494 BglI GCCn_nnn'nGGC Cuts at: 0 1222 1276 1330 1896 Size: 1222 54 54 566 Fragments arranged by size: 1222 566 54 54 BglII A'GATC_T Cuts at: 0 287 1896 Size: 287 1609 BlpI GC'TnA_GC Cuts at: 0 354 1896 Size: 354 1542 Bme1580I G_kGCm'C Cuts at: 0 122 243 1896 Size: 122 121 1653 Fragments arranged by size: 1653 122 121 BmrI ACTGGGnnnn_n' Cuts at: 0 71 1535 1887 1896 Size: 71 1464 352 9 Fragments arranged by size: 1464 352 71 9 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 126 1896 Size: 126 1770 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1051 1358 1896 Size: 1051 307 538 Fragments arranged by size: 1051 538 307 Bpu10I CC'TnA_GC Cuts at: 0 1294 1348 1896 Size: 1294 54 548 Fragments arranged by size: 1294 548 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1530 1896 Size: 1530 366 BsaI GGTCTCn'nnnn_ Cuts at: 0 216 361 1092 1896 Size: 216 145 731 804 Fragments arranged by size: 804 731 216 145 BsaHI Gr'CG_yC Cuts at: 0 1077 1252 1896 Size: 1077 175 644 Fragments arranged by size: 1077 644 175 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 99 691 1896 Size: 99 592 1205 Fragments arranged by size: 1205 592 99 BseYI C'CCAG_C Cuts at: 0 1206 1271 1592 1896 Size: 1206 65 321 304 Fragments arranged by size: 1206 321 304 65 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1174 1896 Size: 1174 722 BsiHKAI G_wGCw'C Cuts at: 0 1516 1694 1765 1896 Size: 1516 178 71 131 Fragments arranged by size: 1516 178 131 71 Bsp1286I G_dGCh'C Cuts at: 0 122 243 1516 1694 1765 1896 Size: 122 121 1273 178 71 131 Fragments arranged by size: 1273 178 131 122 121 71 BsrDI GCAATG_nn' Cuts at: 0 1112 1896 Size: 1112 784 BssSI C'ACGA_G Cuts at: 0 273 997 1896 Size: 273 724 899 Fragments arranged by size: 899 724 273 BstXI CCAn_nnnn'nTGG Cuts at: 0 1471 1534 1896 Size: 1471 63 362 Fragments arranged by size: 1471 362 63 BstYI r'GATC_y Cuts at: 0 287 555 1896 Size: 287 268 1341 Fragments arranged by size: 1341 287 268 BstZ17I GTA'TAC Cuts at: 0 1884 1896 Size: 1884 12 BtgI C'CryG_G Cuts at: 0 1464 1470 1527 1533 1563 1701 1896 Size: 1464 6 57 6 30 138 195 Fragments arranged by size: 1464 195 138 57 30 6 6 BtsI GCAGTG_nn' Cuts at: 0 35 1896 Size: 35 1861 EaeI y'GGCC_r Cuts at: 0 1277 1331 1467 1530 1704 1896 Size: 1277 54 136 63 174 192 Fragments arranged by size: 1277 192 174 136 63 54 EarI CTCTTCn'nnn_ Cuts at: 0 145 1101 1846 1896 Size: 145 956 745 50 Fragments arranged by size: 956 745 145 50 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 205 1896 Size: 205 1691 EcoICRI GAG'CTC Cuts at: 0 1514 1692 1896 Size: 1514 178 204 Fragments arranged by size: 1514 204 178 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 790 1051 1358 1793 1896 Size: 790 261 307 435 103 Fragments arranged by size: 790 435 307 261 103 EcoO109I rG'GnC_Cy Cuts at: 0 50 417 1069 1215 1323 1596 1674 1896 Size: 50 367 652 146 108 273 78 222 Fragments arranged by size: 652 367 273 222 146 108 78 50 EcoRV GAT'ATC Cuts at: 0 854 987 1896 Size: 854 133 909 Fragments arranged by size: 909 854 133 HaeII r_GCGC'y Cuts at: 0 1080 1527 1896 Size: 1080 447 369 Fragments arranged by size: 1080 447 369 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 197 229 1345 1377 1708 1740 1813 1845 Size: 197 32 1116 32 331 32 73 32 Cuts at: 1845 1896 Size: 51 Fragments arranged by size: 1116 331 197 73 51 32 32 32 32 HincII GTy'rAC Cuts at: 0 795 1821 1896 Size: 795 1026 75 Fragments arranged by size: 1026 795 75 HpaI GTT'AAC Cuts at: 0 795 1896 Size: 795 1101 KasI G'GCGC_C Cuts at: 0 1076 1896 Size: 1076 820 MfeI C'AATT_G Cuts at: 0 896 1896 Size: 896 1000 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 549 1896 Size: 549 1347 MscI TGG'CCA Cuts at: 0 1279 1333 1469 1532 1706 1896 Size: 1279 54 136 63 174 190 Fragments arranged by size: 1279 190 174 136 63 54 MslI CAynn'nnrTG Cuts at: 0 500 667 1388 1469 1487 1532 1896 Size: 500 167 721 81 18 45 364 Fragments arranged by size: 721 500 364 167 81 45 18 MspA1I CmG'CkG Cuts at: 0 1275 1896 Size: 1275 621 NarI GG'CG_CC Cuts at: 0 1077 1896 Size: 1077 819 NcoI C'CATG_G Cuts at: 0 1464 1470 1527 1533 1563 1701 1896 Size: 1464 6 57 6 30 138 195 Fragments arranged by size: 1464 195 138 57 30 6 6 NdeI CA'TA_TG Cuts at: 0 1889 1896 Size: 1889 7 NsiI A_TGCA'T Cuts at: 0 404 1896 Size: 404 1492 NspI r_CATG'y Cuts at: 0 18 1896 Size: 18 1878 PasI CC'CwG_GG Cuts at: 0 1756 1896 Size: 1756 140 PflMI CCAn_nnn'nTGG Cuts at: 0 1137 1756 1896 Size: 1137 619 140 Fragments arranged by size: 1137 619 140 PpuMI rG'GwC_Cy Cuts at: 0 50 417 1069 1596 1896 Size: 50 367 652 527 300 Fragments arranged by size: 652 527 367 300 50 PstI C_TGCA'G Cuts at: 0 885 1896 Size: 885 1011 PvuII CAG'CTG Cuts at: 0 1275 1896 Size: 1275 621 SacI G_AGCT'C Cuts at: 0 1516 1694 1896 Size: 1516 178 202 Fragments arranged by size: 1516 202 178 SalI G'TCGA_C Cuts at: 0 1819 1896 Size: 1819 77 SanDI GG'GwC_CC Cuts at: 0 417 1896 Size: 417 1479 SexAI A'CCwGG_T Cuts at: 0 593 1896 Size: 593 1303 SfcI C'TryA_G Cuts at: 0 536 881 1059 1896 Size: 536 345 178 837 Fragments arranged by size: 837 536 345 178 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1276 1330 1896 Size: 1276 54 566 Fragments arranged by size: 1276 566 54 SfoI GGC'GCC Cuts at: 0 1078 1896 Size: 1078 818 SmlI C'TyrA_G Cuts at: 0 1509 1896 Size: 1509 387 SphI G_CATG'C Cuts at: 0 18 1896 Size: 18 1878 StuI AGG'CCT Cuts at: 0 586 1896 Size: 586 1310 StyI C'CwwG_G Cuts at: 0 1084 1464 1470 1527 1533 1563 1701 1896 Size: 1084 380 6 57 6 30 138 195 Fragments arranged by size: 1084 380 195 138 57 30 6 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 240 512 1748 1896 Size: 240 272 1236 148 Fragments arranged by size: 1236 272 240 148 Tth111I GACn'n_nGTC Cuts at: 0 1183 1807 1896 Size: 1183 624 89 Fragments arranged by size: 1183 624 89 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1540 1896 Size: 1540 356 XmnI GAAnn'nnTTC Cuts at: 0 139 1896 Size: 139 1757 ZraI GAC'GTC Cuts at: 0 1253 1896 Size: 1253 643 Enzymes that do cut: AatII AccI AcuI AhdI AloI AlwNI ApoI AvrII BaeI BanI BanII BbeI BbsI BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI BssSI BstXI BstYI BstZ17I BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII HpaI KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NdeI NsiI NspI PasI PflMI PpuMI PstI PvuII SacI SalI SanDI SexAI SfcI SfiI SfoI SmlI SphI StuI StyI TaqII Tth111I XcmI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI BamHI BciVI BclI BmgBI BmtI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRI FalI FseI FspI FspAI HindIII KpnI MluI NaeI NgoMIV NheI NotI NruI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SapI SbfI ScaI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI TstI XbaI XhoI XmaI