Restriction map for
pFN21AE3044
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BbvI BciVI
BclI BcnI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsiYI BslI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoT22I FauI
FbaI Fnu4HI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI ItaI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
TseI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 161 AAATTC [GA]^AATT[CT]
AfaI 530 GTAC GT^AC
AluI 396 AGCT AG^CT
AlwI 354 GGATC GGATC(4/5)
AlwNI 176 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 161 AAATTC [GA]^AATT[CT]
AspHI 395 GAGCTC G[AT]GC[AT]^C
BanII 395 GAGCTC G[GA]GC[CT]^C
BbsI 381 GTCTTC GAAGAC(2/6)
BcgI 122 GCAAAGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 337 CTAG C^TAG
BglII 204 AGATCT A^GATCT
BmyI 395 GAGCTC G[AGT]GC[ACT]^C
BpuAI 381 GTCTTC GAAGAC(2/6)
BsaJI 225 CCAAGG C^C[ACGT][ACGT]GG
BsgI 61 GTGCAG GTGCAG(16/14)
BsiHKAI 395 GAGCTC G[AT]GC[AT]^C
BsmAI 430 GAGAC GTCTC(1/5)
Bsp1286I 395 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 529 TGTACA T^GTACA
BsrGI 529 TGTACA T^GTACA
BsrI 73 ACTGG ACTGG(1/-1)
EaeI 222 CGGCCA [CT]^GGCC[GA]
EcoRV 274 GATATC GAT^ATC
EcoT14I 225 CCAAGG C^C[AT][AT]GG
HaeIII 223 GGCC GG^CC
HapII 508 CCGG C^CGG
HinfI 139 GACTC G^A[ACGT]TC
HpaII 508 CCGG C^CGG
MaeI 337 CTAG C^TAG
MaeIII 70 GTCAC ^GT[ACGT]AC
MseI 254 TTAA T^TAA
MspI 508 CCGG C^CGG
MwoI 507 GCCGGAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 360 GGAACC GG[ACGT]^[ACGT]CC
PleI 139 GACTC GAGTC(4/5)
RsaI 530 GTAC GT^AC
SacI 395 GAGCTC GAGCT^C
SmlI 404 CTCAAG C^T[CT][GA]AG
SspBI 529 TGTACA T^GTACA
StyI 225 CCAAGG C^C[AT][AT]GG
TaqI 131 TCGA T^CGA
Tru9I 254 TTAA T^TAA
Tsp45I 70 GTCAC ^GT[GC]AC
TthHB8I 131 TCGA T^CGA
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AciI 120 CCGC CCGC(-3/-1)
173 CCGC
BsmFI 76 GGGAC GGGAC(10/14)
325 GTCCC
BssKI 237 CCTGG ^CC[ACGT]GG
249 CCTGG
BstNI 237 CCTGG CC^[AT]GG
249 CCTGG
BstYI 204 AGATCT [GA]^GATC[CT]
354 GGATCT
DdeI 182 CTGAG C^T[ACGT]AG
473 CTGAG
EarI 141 CTCTTC CTCTTC(1/4)
309 GAAGAG
EcoRII 237 CCTGG ^CC[AT]GG
249 CCTGG
Ksp632I 141 CTCTTC CTCTTC(1/4)
309 GAAGAG
MflI 204 AGATCT [GA]^GATC[CT]
354 GGATCT
MvaI 237 CCTGG CC^[AT]GG
249 CCTGG
NlaIII 198 CATG CATG^
426 CATG
ScrFI 237 CCTGG CC^[ACGT]GG
249 CCTGG
Tsp509I 162 AATT ^AATT
297 AATT
TspRI 72 CACTG CA[GC]TG[ACGT][ACGT]^
523 CAGTG
XhoII 204 AGATCT [GA]^GATC[CT]
354 GGATCT
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
BstF5I DpnII FokI MboI MboII
MnlI NdeII Sau3AI