################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII Acc65I AclI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BfaI BglI BglII BlnI BsaAI BsaI BsiWI BsmBI BspHI BssHII Bst1107I BstBI BstZ17I Bsu36I CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 997 GTCTAC GT^[AC][GT]AC AccII 1315 CGCG CG^CG AccIII 1566 TCCGGA T^CCGGA AfaI 930 GTAC GT^AC AflII 750 CTTAAG C^TTAAG AflIII 932 ACATGT A^C[GA][CT]GT AgeI 427 ACCGGT A^CCGGT AhdI 1097 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1382 GTGCAC G^TGCAC ApaLI 1382 GTGCAC G^TGCAC AspEI 1097 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 661 GGATCC G^GATCC BanI 1281 GGTGCC G^G[CT][GA]CC BbsI 589 GAAGAC GAAGAC(2/6) BfrI 750 CTTAAG C^TTAAG BpuAI 589 GAAGAC GAAGAC(2/6) BsaBI 844 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1566 TCCGGA T^CCGGA BsiEI 129 CGACCG CG[GA][CT]^CG BsmFI 1105 GTCCC GGGAC(10/14) BsmI 11 GCATTC GAATGC(1/-1) Bsp1407I 929 TGTACA T^GTACA BspDI 150 ATCGAT AT^CGAT BspEI 1566 TCCGGA T^CCGGA BspLU11I 932 ACATGT A^CATGT BsrBI 1547 GAGCGG CCGCTC(-3/-3) BsrDI 1583 CATTGC GCAATG(2/0) BsrFI 427 ACCGGT [GA]^CCGG[CT] BsrGI 929 TGTACA T^GTACA BssSI 211 CTCGTG CACGAG(-5/-1) BstEII 1552 GGTAACC G^GT[ACGT]ACC BstPI 1552 GGTAACC G^GT[ACGT]ACC BstUI 1315 CGCG CG^CG BstXI 1127 CCACTAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 427 ACCGGT [GA]^CCGG[CT] ClaI 150 ATCGAT AT^CGAT DraI 957 TTTAAA TTT^AAA DsaI 543 CCGTGG C^C[GA][CT]GG Eam1105I 1097 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1552 GGTAACC G^GT[ACGT]ACC EcoRI 235 GAATTC G^AATTC HaeII 488 GGCGCT [GA]GCGC^[CT] HphI 363 GGTGA GGTGA(8/7) MaeII 458 ACGT A^CGT MamI 844 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1566 TCCGGA T^CCGGA MspA1I 1502 CAGCTG C[AC]G^C[GT]G MvnI 1315 CGCG CG^CG NspI 932 ACATGT [GA]CATG^[CT] PinAI 427 ACCGGT A^CCGGT PpuMI 356 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 1502 CAGCTG CAG^CTG RsaI 930 GTAC GT^AC SacI 251 GAGCTC GAGCT^C SspBI 929 TGTACA T^GTACA Tsp45I 896 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcyI 548 GGCGTC G[GA]^CG[CT]C 1621 GGCGTC AspHI 251 GAGCTC G[AT]GC[AT]^C 1382 GTGCAC BalI 215 TGGCCA TGG^CCA 645 TGGCCA BclI 819 TGATCA T^GATCA 1215 TGATCA BlpI 580 GCTGAGC GC^T[ACGT]AGC 1309 GCTAAGC BpmI 172 CTGGAG CTGGAG(16/14) 1030 CTGGAG Bpu1102I 580 GCTGAGC GC^T[ACGT]AGC 1309 GCTAAGC BsaHI 548 GGCGTC G[GA]^CG[CT]C 1621 GGCGTC BsaWI 427 ACCGGT [AT]^CCGG[AT] 1566 TCCGGA BsiHKAI 251 GAGCTC G[AT]GC[AT]^C 1382 GTGCAC BsmAI 193 GAGAC GTCTC(1/5) 680 GTCTC BspMI 411 GCAGGT ACCTGC(4/8) 1302 GCAGGT BstAPI 609 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 789 GCAGCGAGTGC CelII 580 GCTGAGC GC^T[ACGT]AGC 1309 GCTAAGC DraII 356 AGGTCCT [GA]G^G[ACGT]CC[CT] 1046 AGGCCCT EcoNI 348 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1272 CCTCTTCCAGG EcoO109I 356 AGGTCCT [GA]G^G[ACGT]CC[CT] 1046 AGGCCCT EcoT14I 278 CCTTGG C^C[AT][AT]GG 1139 CCTTGG FbaI 819 TGATCA T^GATCA 1215 TGATCA Hin1I 548 GGCGTC G[GA]^CG[CT]C 1621 GGCGTC MaeIII 896 GTGAC ^GT[ACGT]AC 1553 GTAAC MfeI 641 CAATTG C^AATTG 1082 CAATTG MluNI 215 TGGCCA TGG^CCA 645 TGGCCA MscI 215 TGGCCA TGG^CCA 645 TGGCCA MseI 751 TTAA T^TAA 958 TTAA MslI 1389 CATAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1531 CATGACGATG MunI 641 CAATTG C^AATTG 1082 CAATTG StyI 278 CCTTGG C^C[AT][AT]GG 1139 CCTTGG TaqI 151 TCGA T^CGA 552 TCGA TfiI 50 GAATC G^A[AT]TC 907 GATTC Tru9I 751 TTAA T^TAA 958 TTAA TthHB8I 151 TCGA T^CGA 552 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AcsI AluI AlwI AlwNI ApoI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BseRI BsgI BsiYI BslI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI Ksp632I MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI TseI Tsp509I TspRI XhoII XmaCI XmaI