(Circular) (Six-base) MAPSORT of: pFN21AE4136.seq Check: 5105 from: 1 to: 6346 pFN21AE4136 6346 bp With 182 enzymes: SgfI * March 28, 2012 13:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4156 278 Size: 53 83 186 936 2620 2468 Fragments arranged by size: 2620 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3003 3689 1937 Size: 1066 686 4594 Fragments arranged by size: 4594 1066 686 Acc65I G'GTAC_C Cuts at: 3667 3667 Size: 6346 AclI AA'CG_TT Cuts at: 1563 4473 4846 1563 Size: 2910 373 3063 Fragments arranged by size: 3063 2910 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2048 2207 3068 3465 4409 5793 6061 1632 Size: 416 159 861 397 944 1384 268 1917 Fragments arranged by size: 1917 1384 944 861 416 397 268 159 AflII C'TTAA_G Cuts at: 820 1017 2755 820 Size: 197 1738 4411 Fragments arranged by size: 4411 1738 197 AflIII A'CryG_T Cuts at: 1203 2937 5245 6222 1203 Size: 1734 2308 977 1327 Fragments arranged by size: 2308 1734 1327 977 AgeI A'CCGG_T Cuts at: 2432 5177 2432 Size: 2745 3601 AhdI GACnn_n'nnGTC Cuts at: 3107 5075 3107 Size: 1968 4378 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6346 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2217 1184 Size: 1033 5313 AlwNI CAG_nnn'CTG Cuts at: 2570 2620 2998 4089 5661 2570 Size: 50 378 1091 1572 3255 Fragments arranged by size: 3255 1572 1091 378 50 ApaLI G'TGCA_C Cuts at: 3387 4403 5559 3387 Size: 1016 1156 4174 Fragments arranged by size: 4174 1156 1016 ApoI r'AATT_y Cuts at: 1493 2240 2634 2819 3655 3881 4033 6142 1493 Size: 747 394 185 836 226 152 2109 1697 Fragments arranged by size: 2109 1697 836 747 394 226 185 152 AseI AT'TA_AT Cuts at: 160 1032 4900 160 Size: 872 3868 1606 Fragments arranged by size: 3868 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6346 AvaI C'yCGr_G Cuts at: 1870 1943 2532 3450 3603 3671 1870 Size: 73 589 918 153 68 4545 Fragments arranged by size: 4545 918 589 153 73 68 BamHI G'GATC_C Cuts at: 2666 3676 4043 2666 Size: 1010 367 4969 Fragments arranged by size: 4969 1010 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3286 3667 5122 618 Size: 325 217 627 1499 381 1455 1842 Fragments arranged by size: 1842 1499 1455 627 381 325 217 BanII G_rGCy'C Cuts at: 1391 2119 2260 2523 2605 3665 1391 Size: 728 141 263 82 1060 4072 Fragments arranged by size: 4072 1060 728 263 141 82 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2601 928 Size: 971 702 4673 Fragments arranged by size: 4673 971 702 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4537 4571 1903 Size: 34 2600 34 3678 Fragments arranged by size: 3678 2600 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4238 5448 4238 Size: 1210 5136 BclI T'GATC_A Cuts at: 1548 2824 3220 1548 Size: 1276 396 4674 Fragments arranged by size: 4674 1276 396 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4957 136 Size: 107 122 71 631 605 3285 1525 Fragments arranged by size: 3285 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6341 6341 Size: 6346 BlpI GC'TnA_GC Cuts at: 2586 3315 3763 2586 Size: 729 448 5169 Fragments arranged by size: 5169 729 448 Bme1580I G_kGCm'C Cuts at: 1790 2044 2051 3391 3603 4407 5563 1790 Size: 254 7 1340 212 804 1156 2573 Fragments arranged by size: 2573 1340 1156 804 254 212 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6346 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3277 4077 5035 Size: 416 53 83 399 24 1860 800 958 Cuts at: 5035 442 Size: 1753 Fragments arranged by size: 1860 1753 958 800 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6346 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2198 3056 5006 1239 Size: 959 858 1950 2579 Fragments arranged by size: 2579 1950 959 858 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2870 3391 4471 5336 5634 2870 Size: 521 1080 865 298 3582 Fragments arranged by size: 3582 1080 865 521 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5009 882 Size: 617 3510 2219 Fragments arranged by size: 3510 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4137 493 Size: 711 2933 2702 Fragments arranged by size: 2933 2702 711 BsaBI GATnn'nnATC Cuts at: 2853 6340 2853 Size: 3487 2859 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2554 3627 4153 4535 Size: 53 83 186 936 1021 1073 526 382 Cuts at: 4535 5170 275 Size: 635 1451 Fragments arranged by size: 1451 1073 1021 936 635 526 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2432 3571 4779 5177 5451 5598 6009 2432 Size: 1139 1208 398 274 147 411 2769 Fragments arranged by size: 2769 1208 1139 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6121 Fragments arranged by size: 6121 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2127 2873 3515 1186 Size: 941 746 642 4017 Fragments arranged by size: 4017 941 746 642 BseYI C'CCAG_C Cuts at: 3211 5549 6045 3211 Size: 2338 496 3512 Fragments arranged by size: 3512 2338 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2583 2813 2862 1163 Size: 573 847 230 49 4647 Fragments arranged by size: 4647 847 573 230 49 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2137 3796 4557 5585 1540 Size: 275 186 136 1659 761 1028 2301 Fragments arranged by size: 2301 1659 1028 761 275 186 136 BsiHKAI G_wGCw'C Cuts at: 2260 3391 4407 4492 5563 2260 Size: 1131 1016 85 1071 3043 Fragments arranged by size: 3043 1131 1071 1016 85 BsmI GAATG_Cn' Cuts at: 2016 3857 3950 2016 Size: 1841 93 4412 Fragments arranged by size: 4412 1841 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2051 2119 2260 2523 2605 3391 Size: 399 254 7 68 141 263 82 786 Cuts at: 3391 3603 3665 4407 4492 5563 1391 Size: 212 62 742 85 1071 2174 Fragments arranged by size: 2174 1071 786 742 399 263 254 212 141 85 82 68 62 7 BspEI T'CCGG_A Cuts at: 3571 6009 3571 Size: 2438 3908 BspHI T'CATG_A Cuts at: 4235 5965 4235 Size: 1730 4616 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2407 3298 3701 844 Size: 1563 891 403 3489 Fragments arranged by size: 3489 1563 891 403 BsrBI CCG'CTC Cuts at: 1394 1823 3554 4233 1394 Size: 429 1731 679 3507 Fragments arranged by size: 3507 1731 679 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3587 4841 5015 65 Size: 1183 2339 1254 174 1396 Fragments arranged by size: 2339 1396 1254 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2432 4990 5177 1700 Size: 110 622 2558 187 2869 Fragments arranged by size: 2869 2558 622 187 110 BsrGI T'GTAC_A Cuts at: 96 2934 96 Size: 2838 3508 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6346 BssSI C'ACGA_G Cuts at: 2216 4406 5418 2216 Size: 2190 1012 3144 Fragments arranged by size: 3144 2190 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2620 2800 794 Size: 1826 180 4340 Fragments arranged by size: 4340 1826 180 BstBI TT'CG_AA Cuts at: 5150 5150 Size: 6346 BstEII G'GTnAC_C Cuts at: 1184 3557 1184 Size: 2373 3973 BstXI CCAn_nnnn'nTGG Cuts at: 3139 3139 Size: 6346 BstYI r'GATC_y Cuts at: 1972 2666 3155 3378 3676 4043 4428 4445 5897 Size: 694 489 223 298 367 385 17 1452 Cuts at: 5897 5983 5995 6341 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 694 489 385 367 346 298 223 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2548 513 Size: 552 939 544 4311 Fragments arranged by size: 4311 939 552 544 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5607 Fragments arranged by size: 5607 683 56 BtsI GCAGTG_nn' Cuts at: 2571 2769 3522 3862 4656 4676 2571 Size: 198 753 340 794 20 4241 Fragments arranged by size: 4241 794 753 340 198 20 ClaI AT'CG_AT Cuts at: 1554 2156 1554 Size: 602 5744 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6311 DraI TTT'AAA Cuts at: 2964 3650 2964 Size: 686 5660 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5353 809 Size: 507 4037 1802 Fragments arranged by size: 4037 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2220 2482 2650 Size: 54 1425 50 275 18 390 262 168 Cuts at: 2650 3793 4682 6311 8 Size: 1143 889 1629 43 Fragments arranged by size: 1629 1425 1143 889 390 275 262 168 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3793 1537 Size: 275 1981 4090 Fragments arranged by size: 4090 1981 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2352 3284 3399 4276 1353 Size: 999 932 115 877 3423 Fragments arranged by size: 3423 999 932 877 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2071 2527 4933 5307 5453 387 Size: 1064 138 482 456 2406 374 146 1280 Fragments arranged by size: 2406 1280 1064 482 456 374 146 138 EcoICRI GAG'CTC Cuts at: 2258 2258 Size: 6346 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2198 2207 3056 3068 3465 4409 Size: 393 416 150 9 849 12 397 944 Cuts at: 4409 5006 5793 6061 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 944 849 787 597 416 397 393 268 150 12 9 EcoNI CCTnn'n_nnAGG Cuts at: 2357 3281 2357 Size: 924 5422 EcoO109I rG'GnC_Cy Cuts at: 2362 3052 2362 Size: 690 5656 EcoRI G'AATT_C Cuts at: 2240 3655 4033 2240 Size: 1415 378 4553 Fragments arranged by size: 4553 1415 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3495 3495 Size: 6346 FspI TGC'GCA Cuts at: 4852 4852 Size: 6346 HaeII r_GCGC'y Cuts at: 1678 2497 5493 1678 Size: 819 2996 2531 Fragments arranged by size: 2996 2531 819 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2217 2249 2674 2706 3093 Size: 32 135 32 645 32 425 32 387 Cuts at: 3093 3125 3147 3179 4987 5019 5061 5093 1373 Size: 32 22 32 1808 32 42 32 2626 Fragments arranged by size: 2626 1808 645 425 387 135 42 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 3690 3936 669 Size: 988 281 1752 246 3079 Fragments arranged by size: 3079 1752 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6346 HpaI GTT'AAC Cuts at: 3936 3936 Size: 6346 KpnI G_GTAC'C Cuts at: 3671 3671 Size: 6346 MfeI C'AATT_G Cuts at: 2646 3087 3945 2646 Size: 441 858 5047 Fragments arranged by size: 5047 858 441 MluI A'CGCG_T Cuts at: 6222 6222 Size: 6346 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5460 5644 1305 Size: 4155 184 2007 Fragments arranged by size: 4155 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2222 2652 10 Size: 54 1425 343 390 430 3704 Fragments arranged by size: 3704 1425 430 390 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3398 3540 4304 4663 4822 518 Size: 648 2232 142 764 359 159 2042 Fragments arranged by size: 2232 2042 764 648 359 159 142 MspA1I CmG'CkG Cuts at: 1606 3509 3762 4439 5587 5832 1606 Size: 1903 253 677 1148 245 2120 Fragments arranged by size: 2120 1903 1148 677 253 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4855 Fragments arranged by size: 4855 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6346 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6346 NotI GC'GGCC_GC Cuts at: 3793 3793 Size: 6346 NspI r_CATG'y Cuts at: 1132 1229 2941 3704 5249 1132 Size: 97 1712 763 1545 2229 Fragments arranged by size: 2229 1712 1545 763 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6346 PciI A'CATG_T Cuts at: 2937 5245 2937 Size: 2308 4038 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6346 PfoI T'CCnGG_A Cuts at: 3197 3197 Size: 6346 PmeI GTTT'AAAC Cuts at: 3650 3650 Size: 6346 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4415 4447 5928 5960 4415 Size: 32 1481 32 4801 Fragments arranged by size: 4801 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2362 2362 Size: 6346 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6346 PsiI TTA'TAA Cuts at: 3916 3916 Size: 6346 PstI C_TGCA'G Cuts at: 830 2616 2625 2994 3698 830 Size: 1786 9 369 704 3478 Fragments arranged by size: 3478 1786 704 369 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6346 PvuII CAG'CTG Cuts at: 3509 3509 Size: 6346 SacI G_AGCT'C Cuts at: 2260 2260 Size: 6346 SalI G'TCGA_C Cuts at: 1936 3688 1936 Size: 1752 4594 SbfI CC_TGCA'GG Cuts at: 3698 3698 Size: 6346 ScaI AGT'ACT Cuts at: 4594 6306 4594 Size: 1712 4634 SfcI C'TryA_G Cuts at: 826 1045 2612 2621 2990 3694 4829 5510 5701 Size: 219 1567 9 369 704 1135 681 191 Cuts at: 5701 826 Size: 1471 Fragments arranged by size: 1567 1471 1135 704 681 369 219 191 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6346 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6346 SmaI CCC'GGG Cuts at: 1872 2534 3452 3605 3673 1872 Size: 662 918 153 68 4545 Fragments arranged by size: 4545 918 662 153 68 SmlI C'TyrA_G Cuts at: 820 1017 1943 2755 2885 3370 4450 5351 5613 Size: 197 926 812 130 485 1080 901 262 Cuts at: 5613 820 Size: 1553 Fragments arranged by size: 1553 1080 926 901 812 485 262 197 130 SnaBI TAC'GTA Cuts at: 493 4137 493 Size: 3644 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6346 SphI G_CATG'C Cuts at: 1132 3704 1132 Size: 2572 3774 SrfI GCCC'GGGC Cuts at: 3452 3452 Size: 6346 SspI AAT'ATT Cuts at: 5 52 4270 5 Size: 47 4218 2081 Fragments arranged by size: 4218 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6346 StyI C'CwwG_G Cuts at: 513 1065 2004 2283 3144 3785 513 Size: 552 939 279 861 641 3074 Fragments arranged by size: 3074 939 861 641 552 279 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 4373 4390 4543 4728 5173 325 Size: 1255 417 2376 17 153 185 445 1498 Fragments arranged by size: 2376 1498 1255 445 417 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2934 4592 6304 Size: 274 80 33 51 1443 957 1658 1712 Cuts at: 6304 96 Size: 138 Fragments arranged by size: 1712 1658 1443 957 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2451 2483 6306 6338 2451 Size: 32 3823 32 2459 Fragments arranged by size: 3823 2459 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6346 XbaI T'CTAG_A Cuts at: 3682 3682 Size: 6346 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6346 XmaI C'CCGG_G Cuts at: 1870 2532 3450 3603 3671 1870 Size: 662 918 153 68 4545 Fragments arranged by size: 4545 918 662 153 68 XmnI GAAnn'nnTTC Cuts at: 4475 4475 Size: 6346 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4154 276 Size: 53 83 186 936 2620 2468 Fragments arranged by size: 2620 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SwaI XcmI