(Linear) (Six-base) MAPSORT of: KIEE4136.seq Check: 7099 from: 1 to: 1641 KIEE4136.orf With 182 enzymes: SgfI * March 22, 2012 17:27 .. AccI GT'mk_AC Cuts at: 0 998 1641 Size: 998 643 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 202 1063 1460 1641 Size: 43 159 861 397 181 Fragments arranged by size: 861 397 181 159 43 AflII C'TTAA_G Cuts at: 0 750 1641 Size: 750 891 AflIII A'CryG_T Cuts at: 0 932 1641 Size: 932 709 AgeI A'CCGG_T Cuts at: 0 427 1641 Size: 427 1214 AhdI GACnn_n'nnGTC Cuts at: 0 1102 1641 Size: 1102 539 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 212 1641 Size: 212 1429 AlwNI CAG_nnn'CTG Cuts at: 0 565 615 993 1641 Size: 565 50 378 648 Fragments arranged by size: 648 565 378 50 ApaLI G'TGCA_C Cuts at: 0 1382 1641 Size: 1382 259 ApoI r'AATT_y Cuts at: 0 235 629 814 1641 Size: 235 394 185 827 Fragments arranged by size: 827 394 235 185 AvaI C'yCGr_G Cuts at: 0 527 1445 1598 1641 Size: 527 918 153 43 Fragments arranged by size: 918 527 153 43 BamHI G'GATC_C Cuts at: 0 661 1641 Size: 661 980 BanI G'GyrC_C Cuts at: 0 1281 1641 Size: 1281 360 BanII G_rGCy'C Cuts at: 0 114 255 518 600 1641 Size: 114 141 263 82 1041 Fragments arranged by size: 1041 263 141 114 82 BbsI GAAGACnn'nnnn_ Cuts at: 0 596 1641 Size: 596 1045 BclI T'GATC_A Cuts at: 0 819 1215 1641 Size: 819 396 426 Fragments arranged by size: 819 426 396 BlpI GC'TnA_GC Cuts at: 0 581 1310 1641 Size: 581 729 331 Fragments arranged by size: 729 581 331 Bme1580I G_kGCm'C Cuts at: 0 39 46 1386 1598 1641 Size: 39 7 1340 212 43 Fragments arranged by size: 1340 212 43 39 7 BmrI ACTGGGnnnn_n' Cuts at: 0 1272 1641 Size: 1272 369 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 193 1051 1641 Size: 193 858 590 Fragments arranged by size: 858 590 193 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 865 1386 1641 Size: 865 521 255 Fragments arranged by size: 865 521 255 BsaBI GATnn'nnATC Cuts at: 0 848 1641 Size: 848 793 BsaHI Gr'CG_yC Cuts at: 0 549 1622 1641 Size: 549 1073 19 Fragments arranged by size: 1073 549 19 BsaWI w'CCGG_w Cuts at: 0 427 1566 1641 Size: 427 1139 75 Fragments arranged by size: 1139 427 75 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 122 868 1510 1641 Size: 122 746 642 131 Fragments arranged by size: 746 642 131 122 BseYI C'CCAG_C Cuts at: 0 1206 1641 Size: 1206 435 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 578 808 857 1641 Size: 578 230 49 784 Fragments arranged by size: 784 578 230 49 BsiEI CG_ry'CG Cuts at: 0 132 1641 Size: 132 1509 BsiHKAI G_wGCw'C Cuts at: 0 255 1386 1641 Size: 255 1131 255 Fragments arranged by size: 1131 255 255 BsmI GAATG_Cn' Cuts at: 0 11 1641 Size: 11 1630 Bsp1286I G_dGCh'C Cuts at: 0 39 46 114 255 518 600 1386 1598 Size: 39 7 68 141 263 82 786 212 Cuts at: 1598 1641 Size: 43 Fragments arranged by size: 786 263 212 141 82 68 43 39 7 BspEI T'CCGG_A Cuts at: 0 1566 1641 Size: 1566 75 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 402 1293 1641 Size: 402 891 348 Fragments arranged by size: 891 402 348 BsrBI CCG'CTC Cuts at: 0 1549 1641 Size: 1549 92 BsrDI GCAATG_nn' Cuts at: 0 1582 1641 Size: 1582 59 BsrFI r'CCGG_y Cuts at: 0 427 1641 Size: 427 1214 BsrGI T'GTAC_A Cuts at: 0 929 1641 Size: 929 712 BssSI C'ACGA_G Cuts at: 0 211 1641 Size: 211 1430 BstAPI GCAn_nnn'nTGC Cuts at: 0 615 795 1641 Size: 615 180 846 Fragments arranged by size: 846 615 180 BstEII G'GTnAC_C Cuts at: 0 1552 1641 Size: 1552 89 BstXI CCAn_nnnn'nTGG Cuts at: 0 1134 1641 Size: 1134 507 BstYI r'GATC_y Cuts at: 0 661 1150 1373 1641 Size: 661 489 223 268 Fragments arranged by size: 661 489 268 223 BtgI C'CryG_G Cuts at: 0 543 1641 Size: 543 1098 BtsI GCAGTG_nn' Cuts at: 0 566 764 1517 1641 Size: 566 198 753 124 Fragments arranged by size: 753 566 198 124 ClaI AT'CG_AT Cuts at: 0 151 1641 Size: 151 1490 DraI TTT'AAA Cuts at: 0 959 1641 Size: 959 682 EaeI y'GGCC_r Cuts at: 0 215 477 645 1641 Size: 215 262 168 996 Fragments arranged by size: 996 262 215 168 EarI CTCTTCn'nnn_ Cuts at: 0 347 1279 1394 1641 Size: 347 932 115 247 Fragments arranged by size: 932 347 247 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 66 522 1641 Size: 66 456 1119 Fragments arranged by size: 1119 456 66 EcoICRI GAG'CTC Cuts at: 0 253 1641 Size: 253 1388 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 193 202 1051 1063 1460 1641 Size: 43 150 9 849 12 397 181 Fragments arranged by size: 849 397 181 150 43 12 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 352 1276 1641 Size: 352 924 365 Fragments arranged by size: 924 365 352 EcoO109I rG'GnC_Cy Cuts at: 0 357 1047 1641 Size: 357 690 594 Fragments arranged by size: 690 594 357 EcoRI G'AATT_C Cuts at: 0 235 1641 Size: 235 1406 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1490 1641 Size: 1490 151 HaeII r_GCGC'y Cuts at: 0 492 1641 Size: 492 1149 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 212 244 669 701 1088 1120 1142 1174 Size: 212 32 425 32 387 32 22 32 Cuts at: 1174 1641 Size: 467 Fragments arranged by size: 467 425 387 212 32 32 32 32 22 MfeI C'AATT_G Cuts at: 0 641 1082 1641 Size: 641 441 559 Fragments arranged by size: 641 559 441 MscI TGG'CCA Cuts at: 0 217 647 1641 Size: 217 430 994 Fragments arranged by size: 994 430 217 MslI CAynn'nnrTG Cuts at: 0 1393 1535 1641 Size: 1393 142 106 Fragments arranged by size: 1393 142 106 MspA1I CmG'CkG Cuts at: 0 1504 1641 Size: 1504 137 NspI r_CATG'y Cuts at: 0 936 1641 Size: 936 705 PciI A'CATG_T Cuts at: 0 932 1641 Size: 932 709 PfoI T'CCnGG_A Cuts at: 0 1192 1641 Size: 1192 449 PpuMI rG'GwC_Cy Cuts at: 0 357 1641 Size: 357 1284 PstI C_TGCA'G Cuts at: 0 611 620 989 1641 Size: 611 9 369 652 Fragments arranged by size: 652 611 369 9 PvuII CAG'CTG Cuts at: 0 1504 1641 Size: 1504 137 SacI G_AGCT'C Cuts at: 0 255 1641 Size: 255 1386 SfcI C'TryA_G Cuts at: 0 607 616 985 1641 Size: 607 9 369 656 Fragments arranged by size: 656 607 369 9 SmaI CCC'GGG Cuts at: 0 529 1447 1600 1641 Size: 529 918 153 41 Fragments arranged by size: 918 529 153 41 SmlI C'TyrA_G Cuts at: 0 750 880 1365 1641 Size: 750 130 485 276 Fragments arranged by size: 750 485 276 130 SrfI GCCC'GGGC Cuts at: 0 1447 1641 Size: 1447 194 StyI C'CwwG_G Cuts at: 0 278 1139 1641 Size: 278 861 502 Fragments arranged by size: 861 502 278 TatI w'GTAC_w Cuts at: 0 929 1641 Size: 929 712 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 446 478 1641 Size: 446 32 1163 Fragments arranged by size: 1163 446 32 XmaI C'CCGG_G Cuts at: 0 527 1445 1598 1641 Size: 527 918 153 43 Fragments arranged by size: 918 527 153 43 Enzymes that do cut: AccI AcuI AflII AflIII AgeI AhdI AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbsI BclI BlpI Bme1580I BmrI BpmI BpuEI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI ClaI DraI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I MfeI MscI MslI MspA1I NspI PciI PfoI PpuMI PstI PvuII SacI SfcI SmaI SmlI SrfI StyI TatI TstI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AleI AloI ApaI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BfrBI BglI BglII BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaXI BsiWI BsmBI BspHI BssHII BstBI BstZ17I Bsu36I BtgZI CspCI DraIII DrdI EagI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmnI ZraI