Restriction map for
pFN21AE3076
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AccI AccII AccIII AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
AvrII BalI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaI BsaWI BseAI BseRI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HapII
HincII HindII HindIII HpaI HpaII
KasI Ksp632I KspI MfeI MluI
MluNI MroI MscI MseI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SspBI SspI
StuI SwaI Tru9I TspRI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 352 GACGTC GACGT^C
Acc65I 175 GGTACC G^GTACC
AclI 287 AACGTT AA^CGTT
AcsI 502 AAATTT [GA]^AATT[CT]
AlwNI 661 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 502 AAATTT [GA]^AATT[CT]
Asp718I 175 GGTACC G^GTACC
AspHI 67 GAGCTC G[AT]GC[AT]^C
BamHI 392 GGATCC G^GATCC
BanII 67 GAGCTC G[GA]GC[CT]^C
BfaI 427 CTAG C^TAG
BsaAI 91 TACGTG [CT]AC^GT[GA]
BsaBI 99 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 44 CGGCCG CG[GA][CT]^CG
BsiHKAI 67 GAGCTC G[AT]GC[AT]^C
BsmAI 535 GAGAC GTCTC(1/5)
BsmI 400 GAATGC GAATGC(1/-1)
BsrDI 404 GCAATG GCAATG(2/0)
BstF5I 12 CATCC GGATG(2/0)
BstXI 203 CCATCGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 4 GCGC GCG^C
DraII 548 AGGACCC [GA]G^G[ACGT]CC[CT]
EagI 44 CGGCCG C^GGCCG
EclXI 44 CGGCCG C^GGCCG
Eco52I 44 CGGCCG C^GGCCG
EcoO109I 548 AGGACCC [GA]G^G[ACGT]CC[CT]
FokI 12 CATCC GGATG(9/13)
HgaI 58 GCGTC GACGC(5/10)
HhaI 4 GCGC GCG^C
HinP1I 4 GCGC G^CGC
KpnI 175 GGTACC GGTAC^C
MaeI 427 CTAG C^TAG
MamI 99 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 47 CCGCTG C[AC]G^C[GT]G
NcoI 21 CCATGG C^CATGG
PflMI 564 CCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 548 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 287 AACGTT AA^CGTT
PstI 31 CTGCAG CTGCA^G
SacI 67 GAGCTC GAGCT^C
SfcI 31 CTGCAG C^T[GA][CT]AG
SmlI 462 CTTGAG C^T[CT][GA]AG
SpeI 426 ACTAGT A^CTAGT
SphI 411 GCATGC GCATG^C
TfiI 99 GATTC G^A[AT]TC
Tsp509I 503 AATT ^AATT
Van91I 564 CCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 57 GGCGTC G[GA]^CG[CT]C
352 GACGTC
AluI 68 AGCT AG^CT
684 AGCT
AvaII 84 GGACC G^G[AT]CC
549 GGACC
BanI 175 GGTACC G^G[CT][GA]CC
480 GGTGCC
BbsI 225 GAAGAC GAAGAC(2/6)
340 GTCTTC
BpmI 64 CTGGAG CTGGAG(16/14)
667 CTGGAG
BpuAI 225 GAAGAC GAAGAC(2/6)
340 GTCTTC
BsaHI 57 GGCGTC G[GA]^CG[CT]C
352 GACGTC
BsgI 94 GTGCAG GTGCAG(16/14)
658 GTGCAG
BspMI 148 ACCTGC ACCTGC(4/8)
277 GCAGGT
BsrI 666 ACTGG ACTGG(1/-1)
87 CCAGT
BstAPI 143 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
411 GCATGCTGTGC
BstYI 392 GGATCC [GA]^GATC[CT]
473 AGATCC
DdeI 492 CTTAG C^T[ACGT]AG
638 CTGAG
EaeI 44 CGGCCG [CT]^GGCC[GA]
542 CGGCCA
EcoT14I 21 CCATGG C^C[AT][AT]GG
545 CCAAGG
FauI 37 CCCGC CCCGC(4/6)
170 GCGGG
Hin1I 57 GGCGTC G[GA]^CG[CT]C
352 GACGTC
HphI 356 TCACC GGTGA(8/7)
374 TCACC
MaeIII 355 GTCAC ^GT[ACGT]AC
458 GTGAC
MflI 392 GGATCC [GA]^GATC[CT]
473 AGATCC
MslI 204 CATCGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
357 CACCAAGATG
StyI 21 CCATGG C^C[AT][AT]GG
545 CCAAGG
Tsp45I 355 GTCAC ^GT[GC]AC
458 GTGAC
XhoII 392 GGATCC [GA]^GATC[CT]
473 AGATCC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AlwI BbvI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BssKI BstNI Cac8I Cfr13I DpnII
DsaI EcoRII Fnu4HI HaeIII HinfI
ItaI MaeII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI TthHB8I
XcmI