Restriction map for
pFN21AE0498
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# NO CUT ENZYMES ( 89 enzymes) #
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Acc65I AccIII AflII AgeI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaWI BseAI BsgI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII BstYI
CelII Cfr10I ClaI CpoI DraIII
DrdI Eco47III EcoT22I FseI HpaI
KasI KpnI MflI MluI MroI
NaeI NarI NgoMIV NheI NruI
NsiI PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RcaI RsrII SbfI ScaI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XbaI XcmI XhoII
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# ONE CUT ENZYMES ( 66 enzymes) #
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AatII 377 GACGTC GACGT^C
AcyI 377 GACGTC G[GA]^CG[CT]C
AflIII 1851 ACGTGT A^C[GA][CT]GT
AhdI 1960 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2674 CCTGAGG CC^T[ACGT]AGG
AspEI 1960 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2849 TGCGCA TGC^GCA
BalI 1484 TGGCCA TGG^CCA
BanII 2392 GAGCTC G[GA]GC[CT]^C
BclI 1137 TGATCA T^GATCA
BsaAI 1850 TACGTG [CT]AC^GT[GA]
BsaBI 2787 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 377 GACGTC G[GA]^CG[CT]C
BsaI 1732 GGTCTC GGTCTC(1/5)
BsmI 2852 GCATTC GAATGC(1/-1)
BspMI 1037 ACCTGC ACCTGC(4/8)
BsrBI 113 GAGCGG CCGCTC(-3/-3)
BsrDI 2717 GCAATG GCAATG(2/0)
BssSI 1147 CTCGTG CACGAG(-5/-1)
Bst1107I 1278 GTATAC GTA^TAC
BstAPI 2272 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1367 TTCGAA TT^CGAA
BstEII 667 GGTTACC G^GT[ACGT]ACC
BstPI 667 GGTTACC G^GT[ACGT]ACC
BstZ17I 1278 GTATAC GTA^TAC
Bsu36I 2674 CCTGAGG CC^T[ACGT]AGG
DraII 761 GGGTCCC [GA]G^G[ACGT]CC[CT]
EagI 116 CGGCCG C^GGCCG
Eam1105I 1960 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 116 CGGCCG C^GGCCG
Eco52I 116 CGGCCG C^GGCCG
Eco81I 2674 CCTGAGG CC^T[ACGT]AGG
EcoNI 2397 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 761 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 667 GGTTACC G^GT[ACGT]ACC
EcoRV 2458 GATATC GAT^ATC
FauI 1608 CCCGC CCCGC(4/6)
FbaI 1137 TGATCA T^GATCA
FspI 2849 TGCGCA TGC^GCA
HaeII 213 AGCGCC [GA]GCGC^[CT]
HapII 86 CCGG C^CGG
HgaI 3041 GACGC GACGC(5/10)
Hin1I 377 GACGTC G[GA]^CG[CT]C
HpaII 86 CCGG C^CGG
MamI 2787 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1484 TGGCCA TGG^CCA
MscI 1484 TGGCCA TGG^CCA
MspI 86 CCGG C^CGG
NcoI 3026 CCATGG C^CATGG
NdeI 527 CATATG CA^TATG
NotI 115 GCGGCCGC GC^GGCCGC
NspI 1565 GCATGT [GA]CATG^[CT]
NspV 1367 TTCGAA TT^CGAA
PaeR7I 2651 CTCGAG C^TCGAG
PpuMI 761 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 2696 CAGCTG CAG^CTG
SacI 2392 GAGCTC GAGCT^C
SfiI 117 GGCCGCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1367 TTCGAA TT^CGAA
SmaI 85 CCCGGG CCC^GGG
SmlI 2651 CTCGAG C^T[CT][GA]AG
SspI 688 AATATT AAT^ATT
Tsp45I 519 GTCAC ^GT[GC]AC
XhoI 2651 CTCGAG C^TCGAG
XmaCI 85 CCCGGG C^CCGGG
XmaI 85 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AclI 178 AACGTT AA^CGTT
2711 AACGTT
AlwI 1815 GGATC GGATC(4/5)
2786 GGATC
Asp700I 1153 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2548 GAAAAACTTC
AspHI 1820 GAGCAC G[AT]GC[AT]^C
2392 GAGCTC
AvaI 85 CCCGGG C^[CT]CG[GA]G
2651 CTCGAG
BcnI 85 CCCGG CC^[GC]GG
86 CCGGG
BfaI 1636 CTAG C^TAG
3017 CTAG
BmyI 1820 GAGCAC G[AGT]GC[ACT]^C
2392 GAGCTC
BpmI 422 CTGGAG CTGGAG(16/14)
1727 CTGGAG
BseRI 106 GAGGAG GAGGAG(10/8)
2677 GAGGAG
BsiHKAI 1820 GAGCAC G[AT]GC[AT]^C
2392 GAGCTC
BsmBI 1861 CGTCTC CGTCTC(1/5)
3039 GAGACG
BsoBI 85 CCCGGG C^[CT]CG[GA]G
2651 CTCGAG
Bsp1286I 1820 GAGCAC G[AGT]GC[ACT]^C
2392 GAGCTC
BstXI 56 CCACTATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
838 CCAGGAGAGTGG
EcoRI 2774 GAATTC G^AATTC
3031 GAATTC
HindIII 964 AAGCTT A^AGCTT
2215 AAGCTT
HphI 1135 GGTGA GGTGA(8/7)
520 TCACC
MaeI 1636 CTAG C^TAG
3017 CTAG
MfeI 653 CAATTG C^AATTG
1931 CAATTG
MunI 653 CAATTG C^AATTG
1931 CAATTG
NciI 85 CCCGG CC^[GC]GG
86 CCGGG
Psp1406I 178 AACGTT AA^CGTT
2711 AACGTT
PstI 1091 CTGCAG CTGCA^G
2099 CTGCAG
SalI 993 GTCGAC G^TCGAC
1589 GTCGAC
SapI 463 GAAGAGC GCTCTTC(1/4)
2488 GAAGAGC
SexAI 920 ACCTGGT A^CC[AT]GGT
1410 ACCTGGT
SfcI 1091 CTGCAG C^T[GA][CT]AG
2099 CTGCAG
XmnI 1153 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2548 GAAAAACTTC
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI ApoI AvaII BbsI BbvI
BglI BpuAI BsaJI BsiEI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HincII
HindII HinfI ItaI Ksp632I KspI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I