Restriction map for
pFN21AE0498
(Circular) (Six-base) MAPSORT of: pFN21AE0498.seq Check: 2998 from: 1 to: 7867
pFN21AE0498 7867 bp
With 182 enzymes: SgfI *
August 14, 2013 14:05 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2386 5677 278
Size: 53 83 186 936 850 3291 2468
Fragments arranged by size:
3291 2468 936 850 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2729 2999 3284 3595 5210 1937
Size: 792 270 285 311 1615 4594
Fragments arranged by size:
4594 1615 792 311 285 270
Acc65I G'GTAC_C
Cuts at: 5188 5188
Size: 7867
AclI AA'CG_TT
Cuts at: 1563 2184 4717 5994 6367 1563
Size: 621 2533 1277 373 3063
Fragments arranged by size:
3063 2533 1277 621 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2321 2322 4563 5930 7314 7582 1632
Size: 689 1 2241 1367 1384 268 1917
Fragments arranged by size:
2241 1917 1384 1367 689 268 1
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7670
AflIII A'CryG_T
Cuts at: 1203 3856 6766 7743 1203
Size: 2653 2910 977 1327
Fragments arranged by size:
2910 2653 1327 977
AgeI A'CCGG_T
Cuts at: 6698 6698
Size: 7867
AhdI GACnn_n'nnGTC
Cuts at: 3970 6596 3970
Size: 2626 5241
AleI CACnn'nnGTG
Cuts at: 1166 2066 1166
Size: 900 6967
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2034 1184
Size: 850 7017
AlwNI CAG_nnn'CTG
Cuts at: 5610 7182 5610
Size: 1572 6295
ApaLI G'TGCA_C
Cuts at: 5924 7080 5924
Size: 1156 6711
ApoI r'AATT_y
Cuts at: 1493 2291 2753 3635 3650 3704 4153 4187 4413
Size: 798 462 882 15 54 449 34 226
Cuts at: 4413 4779 5036 5150 5176 5402 5554 7663 1493
Size: 366 257 114 26 226 152 2109 1697
Fragments arranged by size:
2109 1697 882 798 462 449 366 257
226 226 152 114 54 34 26 15
AseI AT'TA_AT
Cuts at: 160 1032 6421 160
Size: 872 5389 1606
Fragments arranged by size:
5389 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7867
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 4656 5192 1870
Size: 73 147 2566 536 4545
Fragments arranged by size:
4545 2566 536 147 73
BamHI G'GATC_C
Cuts at: 5197 5564 5197
Size: 367 7500
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5188 6643 618
Size: 325 217 627 3401 1455 1842
Fragments arranged by size:
3401 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4401 5186 1391
Size: 3010 785 4072
Fragments arranged by size:
4072 3010 785
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3961 4071 4106 4134 4436 928
Size: 971 2062 110 35 28 302 4359
Fragments arranged by size:
4359 2062 971 302 110 35 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6058 6092 1903
Size: 34 4121 34 3678
Fragments arranged by size:
4121 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5759 6969 5759
Size: 1210 6657
BclI T'GATC_A
Cuts at: 1548 3142 1548
Size: 1594 6273
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2027 2039 2129
Size: 107 122 71 631 605 355 12 90
Cuts at: 2129 2147 6478 136
Size: 18 4331 1525
Fragments arranged by size:
4331 1525 631 605 355 122 107 90
71 18 12
BglII A'GATC_T
Cuts at: 7862 7862
Size: 7867
BlpI GC'TnA_GC
Cuts at: 5284 5284
Size: 7867
Bme1580I G_kGCm'C
Cuts at: 1790 5928 7084 1790
Size: 4138 1156 2573
Fragments arranged by size:
4138 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7867
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4987 5598 6556
Size: 416 53 83 399 24 3570 611 958
Cuts at: 6556 442
Size: 1753
Fragments arranged by size:
3570 1753 958 611 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7867
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2448 3753 6527 1239
Size: 1209 1305 2774 2579
Fragments arranged by size:
2774 2579 1305 1209
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5992 6857 7155 5992
Size: 865 298 6704
Fragments arranged by size:
6704 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3743 6530 882
Size: 617 2244 2787 2219
Fragments arranged by size:
2787 2244 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3857 5658 493
Size: 711 2653 1801 2702
Fragments arranged by size:
2702 2653 1801 711
BsaBI GATnn'nnATC
Cuts at: 4796 7861 4796
Size: 3065 4802
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2383 5674 6056 6691
Size: 53 83 186 936 850 3291 382 635
Cuts at: 6691 275
Size: 1451
Fragments arranged by size:
3291 1451 936 850 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6300 6698 6972 7119 7530 6300
Size: 398 274 147 411 6637
Fragments arranged by size:
6637 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5122 5152 1178
Size: 30 165 30 3719 30 3893
Fragments arranged by size:
3893 3719 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 4697 1186
Size: 940 2571 4356
Fragments arranged by size:
4356 2571 940
BseYI C'CCAG_C
Cuts at: 7070 7566 7070
Size: 496 7371
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7294
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2058 2124 2275 5317 6078 7106
Size: 275 186 57 66 151 3042 761 1028
Cuts at: 7106 1540
Size: 2301
Fragments arranged by size:
3042 2301 1028 761 275 186 151 66
57
BsiHKAI G_wGCw'C
Cuts at: 3829 4401 5928 6013 7084 3829
Size: 572 1527 85 1071 4612
Fragments arranged by size:
4612 1527 1071 572 85
BsmI GAATG_Cn'
Cuts at: 4857 5378 5471 4857
Size: 521 93 7253
Fragments arranged by size:
7253 521 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3872 5038 3872
Size: 1166 6701
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3829 4401 5186 5928 6013 7084 1391
Size: 399 2039 572 785 742 85 1071 2174
Fragments arranged by size:
2174 2039 1071 785 742 572 399 85
BspEI T'CCGG_A
Cuts at: 7530 7530
Size: 7867
BspHI T'CATG_A
Cuts at: 5756 7486 5756
Size: 1730 6137
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3051 5222 844
Size: 2207 2171 3489
Fragments arranged by size:
3489 2207 2171
BsrBI CCG'CTC
Cuts at: 1394 1823 2120 5754 1394
Size: 429 297 3634 3507
Fragments arranged by size:
3634 3507 429 297
BsrDI GCAATG_nn'
Cuts at: 65 1248 4729 6362 6536 65
Size: 1183 3481 1633 174 1396
Fragments arranged by size:
3481 1633 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6511 6698 1700
Size: 110 4701 187 2869
Fragments arranged by size:
4701 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7867
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7867
BssSI C'ACGA_G
Cuts at: 3152 5927 6939 3152
Size: 2775 1012 4080
Fragments arranged by size:
4080 2775 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4283 794
Size: 3489 4378
BstBI TT'CG_AA
Cuts at: 3373 6671 3373
Size: 3298 4569
BstEII G'GTnAC_C
Cuts at: 1184 2672 1184
Size: 1488 6379
BstXI CCAn_nnnn'nTGG
Cuts at: 2068 2850 2068
Size: 782 7085
BstYI r'GATC_y
Cuts at: 1972 5197 5564 5949 5966 7418 7504 7516 7862
Size: 3225 367 385 17 1452 86 12 346
Cuts at: 7862 1972
Size: 1977
Fragments arranged by size:
3225 1977 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3285 3285
Size: 7867
Bsu36I CC'TnA_GG
Cuts at: 4680 4680
Size: 7867
BtgI C'CryG_G
Cuts at: 513 1065 2004 2037 2046 2127 2145 2151 4337
Size: 552 939 33 9 81 18 6 2186
Cuts at: 4337 5031 513
Size: 694 3349
Fragments arranged by size:
3349 2186 939 694 552 81 33 18
9 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7128
Fragments arranged by size:
7128 683 56
BtsI GCAGTG_nn'
Cuts at: 2016 2031 4284 5383 6177 6197 2016
Size: 15 2253 1099 794 20 3686
Fragments arranged by size:
3686 2253 1099 794 20 15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7867
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7832
DraI TTT'AAA
Cuts at: 2758 3177 3318 5171 2758
Size: 419 141 1853 5454
Fragments arranged by size:
5454 1853 419 141
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6874 809
Size: 507 5558 1802
Fragments arranged by size:
5558 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2130 2148
Size: 54 1425 50 275 18 291 9 18
Cuts at: 2148 3489 5314 6203 7832 8
Size: 1341 1825 889 1629 43
Fragments arranged by size:
1825 1629 1425 1341 889 291 275 54
50 43 18 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2121 5314 1537
Size: 275 309 3193 4090
Fragments arranged by size:
4090 3193 309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2250 2463 3212 3328 3885 4488 4530 4627
Size: 897 213 749 116 557 603 42 97
Cuts at: 4627 5797 1353
Size: 1170 3423
Fragments arranged by size:
3423 1170 897 749 603 557 213 116
97 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6454 6828 6974 387
Size: 1064 138 4865 374 146 1280
Fragments arranged by size:
4865 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4399 4399
Size: 7867
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2321 2322 2448 3753 4563 5930 6527
Size: 393 689 1 126 1305 810 1367 597
Cuts at: 6527 7314 7582 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1367 1305 810 787 689 597 393
268 126 1
EcoNI CCTnn'n_nnAGG
Cuts at: 4406 4406
Size: 7867
EcoO109I rG'GnC_Cy
Cuts at: 2767 2767
Size: 7867
EcoRI G'AATT_C
Cuts at: 4779 5036 5176 5554 4779
Size: 257 140 378 7092
Fragments arranged by size:
7092 378 257 140
EcoRV GAT'ATC
Cuts at: 4465 4465
Size: 7867
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2431 3268 2431
Size: 837 7030
FspI TGC'GCA
Cuts at: 4856 6373 4856
Size: 1517 6350
HaeII r_GCGC'y
Cuts at: 1678 2222 7014 1678
Size: 544 4792 2531
Fragments arranged by size:
4792 2531 544
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2462 2494 2545 2577 2913
Size: 32 135 32 890 32 51 32 336
Cuts at: 2913 2945 2981 3013 3956 3988 4231 4263 4658
Size: 32 36 32 943 32 243 32 395
Cuts at: 4658 4690 6508 6540 6582 6614 1373
Size: 32 1818 32 42 32 2626
Fragments arranged by size:
2626 1818 943 890 395 336 243 135
51 42 36 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 3596 4056 5211 5457 669
Size: 988 281 1062 596 460 1155 246 3079
Fragments arranged by size:
3079 1155 1062 988 596 460 281 246
HindIII A'AGCT_T
Cuts at: 748 2969 4220 748
Size: 2221 1251 4395
Fragments arranged by size:
4395 2221 1251
HpaI GTT'AAC
Cuts at: 5457 5457
Size: 7867
KpnI G_GTAC'C
Cuts at: 5192 5192
Size: 7867
MfeI C'AATT_G
Cuts at: 2658 3936 5466 2658
Size: 1278 1530 5059
Fragments arranged by size:
5059 1530 1278
MluI A'CGCG_T
Cuts at: 7743 7743
Size: 7867
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2378 2468 2776 6981 7165 1305
Size: 1073 90 308 4205 184 2007
Fragments arranged by size:
4205 2007 1073 308 184 90
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3491 10
Size: 54 1425 343 1659 4386
Fragments arranged by size:
4386 1659 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2066 3362 3713 5825 6184 6343 518
Size: 648 900 1296 351 2112 359 159 2042
Fragments arranged by size:
2112 2042 1296 900 648 359 351 159
MspA1I CmG'CkG
Cuts at: 1606 2048 2129 2147 4703 5283 5960 7108 7353
Size: 442 81 18 2556 580 677 1148 245
Cuts at: 7353 1606
Size: 2120
Fragments arranged by size:
2556 2120 1148 677 580 442 245 81
18
NcoI C'CATG_G
Cuts at: 513 1065 2004 5031 513
Size: 552 939 3027 3349
Fragments arranged by size:
3349 3027 939 552
NdeI CA'TA_TG
Cuts at: 387 2533 387
Size: 2146 5721
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7867
NotI GC'GGCC_GC
Cuts at: 2121 5314 2121
Size: 3193 4674
NspI r_CATG'y
Cuts at: 1132 1229 3574 5225 6770 1132
Size: 97 2345 1651 1545 2229
Fragments arranged by size:
2345 2229 1651 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7867
PciI A'CATG_T
Cuts at: 6766 6766
Size: 7867
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7867
PfoI T'CCnGG_A
Cuts at: 2698 4401 2698
Size: 1703 6164
PmeI GTTT'AAAC
Cuts at: 5171 5171
Size: 7867
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5936 5968 7449 7481 5936
Size: 32 1481 32 6322
Fragments arranged by size:
6322 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2767 2767
Size: 7867
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7867
PsiI TTA'TAA
Cuts at: 5437 5437
Size: 7867
PstI C_TGCA'G
Cuts at: 830 3100 4108 5219 830
Size: 2270 1008 1111 3478
Fragments arranged by size:
3478 2270 1111 1008
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7867
PvuII CAG'CTG
Cuts at: 4703 4703
Size: 7867
SacI G_AGCT'C
Cuts at: 4401 4401
Size: 7867
SacII CC_GC'GG
Cuts at: 2049 2130 2148 2049
Size: 81 18 7768
Fragments arranged by size:
7768 81 18
SalI G'TCGA_C
Cuts at: 1936 2998 3594 5209 1936
Size: 1062 596 1615 4594
Fragments arranged by size:
4594 1615 1062 596
SanDI GG'GwC_CC
Cuts at: 2767 2767
Size: 7867
SapI GCTCTTCn'nnn_
Cuts at: 2463 4488 2463
Size: 2025 5842
SbfI CC_TGCA'GG
Cuts at: 5219 5219
Size: 7867
ScaI AGT'ACT
Cuts at: 6115 7827 6115
Size: 1712 6155
SexAI A'CCwGG_T
Cuts at: 2925 3415 2925
Size: 490 7377
SfcI C'TryA_G
Cuts at: 826 1045 3096 4104 5215 6350 7031 7222 826
Size: 219 2051 1008 1111 1135 681 191 1471
Fragments arranged by size:
2051 1471 1135 1111 1008 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2129 2129
Size: 7867
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7867
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7867
SmaI CCC'GGG
Cuts at: 1872 2092 5194 1872
Size: 220 3102 4545
Fragments arranged by size:
4545 3102 220
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4656 5971 6872 7134 820
Size: 197 926 2713 1315 901 262 1553
Fragments arranged by size:
2713 1553 1315 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5658 493
Size: 5165 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7867
SphI G_CATG'C
Cuts at: 1132 5225 1132
Size: 4093 3774
SrfI GCCC'GGGC
Cuts at: 2092 2092
Size: 7867
SspI AAT'ATT
Cuts at: 5 52 2695 5791 5
Size: 47 2643 3096 2081
Fragments arranged by size:
3096 2643 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7867
StyI C'CwwG_G
Cuts at: 513 1065 2004 2638 4451 5031 5306 513
Size: 552 939 634 1813 580 275 3074
Fragments arranged by size:
3074 1813 939 634 580 552 275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2289 4040 5894 5911 6064 6249 6694
Size: 1255 709 1751 1854 17 153 185 445
Cuts at: 6694 325
Size: 1498
Fragments arranged by size:
1854 1751 1498 1255 709 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6113 7825 96
Size: 274 80 33 51 1443 4136 1712 138
Fragments arranged by size:
4136 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7827 7859 7827
Size: 32 7835
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7867
XbaI T'CTAG_A
Cuts at: 5203 5203
Size: 7867
XhoI C'TCGA_G
Cuts at: 1943 4656 1943
Size: 2713 5154
XmaI C'CCGG_G
Cuts at: 1870 2090 5192 1870
Size: 220 3102 4545
Fragments arranged by size:
4545 3102 220
XmnI GAAnn'nnTTC
Cuts at: 3162 4557 5996 3162
Size: 1395 1439 5033
Fragments arranged by size:
5033 1439 1395
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2384 5675 276
Size: 53 83 186 936 850 3291 2468
Fragments arranged by size:
3291 2468 936 850 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI DraIII FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SfoI SwaI XcmI