Restriction map for
pFN21AE0498
(Linear) (Six-base) MAPSORT of: KIEE0498.seq Check: 8412 from: 1 to: 3162
KIEE0498.orf
With 182 enzymes: SgfI *
August 14, 2013 11:53 ..
AatII G_ACGT'C
Cuts at: 0 381 3162
Size: 381 2781
AccI GT'mk_AC
Cuts at: 0 724 994 1279 1590 3162
Size: 724 270 285 311 1572
Fragments arranged by size:
1572 724 311 285 270
AclI AA'CG_TT
Cuts at: 0 179 2712 3162
Size: 179 2533 450
Fragments arranged by size:
2533 450 179
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 317 2558 3162
Size: 316 1 2241 604
Fragments arranged by size:
2241 604 316 1
AflIII A'CryG_T
Cuts at: 0 1851 3162
Size: 1851 1311
AhdI GACnn_n'nnGTC
Cuts at: 0 1965 3162
Size: 1965 1197
AleI CACnn'nnGTG
Cuts at: 0 61 3162
Size: 61 3101
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 29 3162
Size: 29 3133
ApoI r'AATT_y
Cuts at: 0 286 748 1630 1645 1699 2148 2182 2408
Size: 286 462 882 15 54 449 34 226
Cuts at: 2408 2774 3031 3145 3162
Size: 366 257 114 17
Fragments arranged by size:
882 462 449 366 286 257 226 114
54 34 17 15
AvaI C'yCGr_G
Cuts at: 0 85 2651 3162
Size: 85 2566 511
Fragments arranged by size:
2566 511 85
BanII G_rGCy'C
Cuts at: 0 2396 3162
Size: 2396 766
BbsI GAAGACnn'nnnn_
Cuts at: 0 1956 2066 2101 2129 2431 3162
Size: 1956 110 35 28 302 731
Fragments arranged by size:
1956 731 302 110 35 28
BclI T'GATC_A
Cuts at: 0 1137 3162
Size: 1137 2025
BglI GCCn_nnn'nGGC
Cuts at: 0 22 34 124 142 3162
Size: 22 12 90 18 3020
Fragments arranged by size:
3020 90 22 18 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 2982 3162
Size: 2982 180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 1748 3162
Size: 443 1305 1414
Fragments arranged by size:
1414 1305 443
BsaI GGTCTCn'nnnn_
Cuts at: 0 1738 3162
Size: 1738 1424
BsaAI yAC'GTr
Cuts at: 0 1852 3162
Size: 1852 1310
BsaBI GATnn'nnATC
Cuts at: 0 2791 3162
Size: 2791 371
BsaHI Gr'CG_yC
Cuts at: 0 378 3162
Size: 378 2784
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3117 3147 3162
Size: 3117 30 15
Fragments arranged by size:
3117 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 2692 3162
Size: 121 2571 470
Fragments arranged by size:
2571 470 121
BsiEI CG_ry'CG
Cuts at: 0 53 119 270 3162
Size: 53 66 151 2892
Fragments arranged by size:
2892 151 66 53
BsiHKAI G_wGCw'C
Cuts at: 0 1824 2396 3162
Size: 1824 572 766
Fragments arranged by size:
1824 766 572
BsmI GAATG_Cn'
Cuts at: 0 2852 3162
Size: 2852 310
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1867 3033 3162
Size: 1867 1166 129
Fragments arranged by size:
1867 1166 129
Bsp1286I G_dGCh'C
Cuts at: 0 1824 2396 3162
Size: 1824 572 766
Fragments arranged by size:
1824 766 572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1046 3162
Size: 1046 2116
BsrBI CCG'CTC
Cuts at: 0 115 3162
Size: 115 3047
BsrDI GCAATG_nn'
Cuts at: 0 2724 3162
Size: 2724 438
BssSI C'ACGA_G
Cuts at: 0 1147 3162
Size: 1147 2015
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2278 3162
Size: 2278 884
BstBI TT'CG_AA
Cuts at: 0 1368 3162
Size: 1368 1794
BstEII G'GTnAC_C
Cuts at: 0 667 3162
Size: 667 2495
BstXI CCAn_nnnn'nTGG
Cuts at: 0 63 845 3162
Size: 63 782 2317
Fragments arranged by size:
2317 782 63
BstZ17I GTA'TAC
Cuts at: 0 1280 3162
Size: 1280 1882
Bsu36I CC'TnA_GG
Cuts at: 0 2675 3162
Size: 2675 487
BtgI C'CryG_G
Cuts at: 0 32 41 122 140 146 2332 3026 3162
Size: 32 9 81 18 6 2186 694 136
Fragments arranged by size:
2186 694 136 81 32 18 9 6
BtsI GCAGTG_nn'
Cuts at: 0 11 26 2279 3162
Size: 11 15 2253 883
Fragments arranged by size:
2253 883 15 11
DraI TTT'AAA
Cuts at: 0 753 1172 1313 3162
Size: 753 419 141 1849
Fragments arranged by size:
1849 753 419 141
EaeI y'GGCC_r
Cuts at: 0 116 125 143 1484 3162
Size: 116 9 18 1341 1678
Fragments arranged by size:
1678 1341 116 18 9
EagI C'GGCC_G
Cuts at: 0 116 3162
Size: 116 3046
EarI CTCTTCn'nnn_
Cuts at: 0 245 458 1207 1323 1880 2483 2525 2622
Size: 245 213 749 116 557 603 42 97
Cuts at: 2622 3162
Size: 540
Fragments arranged by size:
749 603 557 540 245 213 116 97
42
EcoICRI GAG'CTC
Cuts at: 0 2394 3162
Size: 2394 768
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 317 443 1748 2558 3162
Size: 316 1 126 1305 810 604
Fragments arranged by size:
1305 810 604 316 126 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2401 3162
Size: 2401 761
EcoO109I rG'GnC_Cy
Cuts at: 0 762 3162
Size: 762 2400
EcoRI G'AATT_C
Cuts at: 0 2774 3031 3162
Size: 2774 257 131
Fragments arranged by size:
2774 257 131
EcoRV GAT'ATC
Cuts at: 0 2460 3162
Size: 2460 702
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 426 1263 3162
Size: 426 837 1899
Fragments arranged by size:
1899 837 426
FspI TGC'GCA
Cuts at: 0 2851 3162
Size: 2851 311
HaeII r_GCGC'y
Cuts at: 0 217 3162
Size: 217 2945
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 457 489 540 572 908 940 976 1008
Size: 457 32 51 32 336 32 36 32
Cuts at: 1008 1951 1983 2226 2258 2653 2685 3162
Size: 943 32 243 32 395 32 477
Fragments arranged by size:
943 477 457 395 336 243 51 36
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 995 1591 2051 3162
Size: 995 596 460 1111
Fragments arranged by size:
1111 995 596 460
HindIII A'AGCT_T
Cuts at: 0 964 2215 3162
Size: 964 1251 947
Fragments arranged by size:
1251 964 947
MfeI C'AATT_G
Cuts at: 0 653 1931 3162
Size: 653 1278 1231
Fragments arranged by size:
1278 1231 653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 463 771 3162
Size: 373 90 308 2391
Fragments arranged by size:
2391 373 308 90
MscI TGG'CCA
Cuts at: 0 1486 3162
Size: 1486 1676
MslI CAynn'nnrTG
Cuts at: 0 61 1357 1708 3162
Size: 61 1296 351 1454
Fragments arranged by size:
1454 1296 351 61
MspA1I CmG'CkG
Cuts at: 0 43 124 142 2698 3162
Size: 43 81 18 2556 464
Fragments arranged by size:
2556 464 81 43 18
NcoI C'CATG_G
Cuts at: 0 3026 3162
Size: 3026 136
NdeI CA'TA_TG
Cuts at: 0 528 3162
Size: 528 2634
NotI GC'GGCC_GC
Cuts at: 0 116 3162
Size: 116 3046
NspI r_CATG'y
Cuts at: 0 1569 3162
Size: 1569 1593
PfoI T'CCnGG_A
Cuts at: 0 693 2396 3162
Size: 693 1703 766
Fragments arranged by size:
1703 766 693
PpuMI rG'GwC_Cy
Cuts at: 0 762 3162
Size: 762 2400
PstI C_TGCA'G
Cuts at: 0 1095 2103 3162
Size: 1095 1008 1059
Fragments arranged by size:
1095 1059 1008
PvuII CAG'CTG
Cuts at: 0 2698 3162
Size: 2698 464
SacI G_AGCT'C
Cuts at: 0 2396 3162
Size: 2396 766
SacII CC_GC'GG
Cuts at: 0 44 125 143 3162
Size: 44 81 18 3019
Fragments arranged by size:
3019 81 44 18
SalI G'TCGA_C
Cuts at: 0 993 1589 3162
Size: 993 596 1573
Fragments arranged by size:
1573 993 596
SanDI GG'GwC_CC
Cuts at: 0 762 3162
Size: 762 2400
SapI GCTCTTCn'nnn_
Cuts at: 0 458 2483 3162
Size: 458 2025 679
Fragments arranged by size:
2025 679 458
SexAI A'CCwGG_T
Cuts at: 0 920 1410 3162
Size: 920 490 1752
Fragments arranged by size:
1752 920 490
SfcI C'TryA_G
Cuts at: 0 1091 2099 3162
Size: 1091 1008 1063
Fragments arranged by size:
1091 1063 1008
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 124 3162
Size: 124 3038
SmaI CCC'GGG
Cuts at: 0 87 3162
Size: 87 3075
SmlI C'TyrA_G
Cuts at: 0 2651 3162
Size: 2651 511
SrfI GCCC'GGGC
Cuts at: 0 87 3162
Size: 87 3075
SspI AAT'ATT
Cuts at: 0 690 3162
Size: 690 2472
StyI C'CwwG_G
Cuts at: 0 633 2446 3026 3162
Size: 633 1813 580 136
Fragments arranged by size:
1813 633 580 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 284 2035 3162
Size: 284 1751 1127
Fragments arranged by size:
1751 1127 284
XhoI C'TCGA_G
Cuts at: 0 2651 3162
Size: 2651 511
XmaI C'CCGG_G
Cuts at: 0 85 3162
Size: 85 3077
XmnI GAAnn'nnTTC
Cuts at: 0 1157 2552 3162
Size: 1157 1395 610
Fragments arranged by size:
1395 1157 610
ZraI GAC'GTC
Cuts at: 0 379 3162
Size: 379 2783
Enzymes that do cut:
AatII AccI AclI AcuI AflIII AhdI AleI AlfI
ApoI AvaI BanII BbsI BclI BglI BmrI BpmI
BsaI BsaAI BsaBI BsaHI BsaXI BseRI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtsI DraI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII MfeI
MmeI MscI MslI MspA1I NcoI NdeI NotI NspI
PfoI PpuMI PstI PvuII SacI SacII SalI SanDI
SapI SexAI SfcI SfiI SmaI SmlI SrfI SspI
StyI TaqII XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflII AgeI AloI AlwNI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbvCI BcgI BciVI BfrBI BglII BlpI Bme1580I
BmgBI BmtI BplI Bpu10I BpuEI BsaWI BseYI BsgI
BsiWI BspEI BspHI BsrFI BsrGI BssHII BstYI BtgZI
ClaI CspCI DraIII DrdI EciI FseI FspAI HpaI
KasI KpnI MluI NaeI NarI NgoMIV NheI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SbfI
ScaI SfoI SgfI SgrAI SnaBI SpeI SphI StuI
SwaI TatI TstI Tth111I XbaI XcmI