################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BmyI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MluI MluNI MnlI MroI MscI MspA1I MspI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AfaI 313 GTAC GT^AC AlwI 142 GATCC GGATC(4/5) BbvI 376 GCTGC GCAGC(8/12) BlpI 361 GCTAAGC GC^T[ACGT]AGC BpmI 356 CTGGAG CTGGAG(16/14) Bpu1102I 361 GCTAAGC GC^T[ACGT]AGC BsaJI 112 CCATGG C^C[ACGT][ACGT]GG BspHI 440 TCATGA T^CATGA BspMI 285 GCAGGT ACCTGC(4/8) BsrI 45 CCAGT ACTGG(1/-1) BstF5I 42 CATCC GGATG(2/0) BstYI 141 AGATCC [GA]^GATC[CT] Cac8I 420 GCATGC GC[ACGT]^[ACGT]GC CelII 361 GCTAAGC GC^T[ACGT]AGC Cfr13I 226 GGGCC G^G[ACGT]CC DpnII 142 GATC ^GATC DraI 507 TTTAAA TTT^AAA DraII 225 AGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 112 CCATGG C^C[GA][CT]GG EcoO109I 225 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 112 CCATGG C^C[AT][AT]GG Fnu4HI 376 GCTGC GC^[ACGT]GC FokI 42 CATCC GGATG(9/13) HaeIII 227 GGCC GG^CC HincII 472 GTCAAC GT[CT]^[GA]AC HindII 472 GTCAAC GT[CT]^[GA]AC HindIII 364 AAGCTT A^AGCTT ItaI 376 GCTGC GC^[ACGT]GC MboI 142 GATC ^GATC MfeI 82 CAATTG C^AATTG MflI 141 AGATCC [GA]^GATC[CT] MslI 441 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 82 CAATTG C^AATTG NcoI 112 CCATGG C^CATGG NdeI 526 CATATG CA^TATG NdeII 142 GATC ^GATC NlaIV 298 GGAGCC GG[ACGT]^[ACGT]CC NspI 420 GCATGC [GA]CATG^[CT] PleI 437 GAGTC GAGTC(4/5) RcaI 440 TCATGA T^CATGA RsaI 313 GTAC GT^AC Sau3AI 142 GATC ^GATC Sau96I 226 GGGCC G^G[ACGT]CC SphI 420 GCATGC GCATG^C StyI 112 CCATGG C^C[AT][AT]GG SwaI 506 ATTTAAAT ATTT^AAAT TfiI 78 GAATC G^A[AT]TC TseI 376 GCTGC G^C[AT]GC TspRI 318 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoII 141 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AcsI 10 AAATTT [GA]^AATT[CT] 307 AAATTT AluI 360 AGCT AG^CT 365 AGCT ApoI 10 AAATTT [GA]^AATT[CT] 307 AAATTT BbsI 457 GAAGAC GAAGAC(2/6) 531 GAAGAC BpuAI 457 GAAGAC GAAGAC(2/6) 531 GAAGAC HinfI 78 GAATC G^A[ACGT]TC 437 GAGTC MwoI 276 GCCACACCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 397 GCTATCATGGC PstI 377 CTGCAG CTGCA^G 452 CTGCAG SfaNI 161 GATGC GCATC(5/9) 41 GCATC SfcI 377 CTGCAG C^T[GA][CT]AG 452 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### BssKI BstNI DdeI EcoRII MboII MseI MvaI NlaIII ScrFI Tru9I Tsp509I