(Linear) (Six-base) MAPSORT of: KIEE5252.seq  Check: 1819  from: 1  to: 543



KIEE5252.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:52  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     543 
   Size:         41     502
 
ApoI r'AATT_y

Cuts at:      0      10     307     543 
   Size:         10     297     236

  Fragments arranged by size: 

                 297     236      10
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     377     410     543 
   Size:        377      33     133

  Fragments arranged by size: 

                 377     133      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     464     538     543 
   Size:        464      74       5

  Fragments arranged by size: 

                 464      74       5
 
BlpI GC'TnA_GC

Cuts at:      0     362     543 
   Size:        362     181
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     220     543 
   Size:        220     323
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     377     543 
   Size:        377     166
 
BspHI T'CATG_A

Cuts at:      0     440     543 
   Size:        440     103
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     276     543 
   Size:        276     267
 
BstYI r'GATC_y

Cuts at:      0     141     543 
   Size:        141     402
 
BtgI C'CryG_G

Cuts at:      0     112     543 
   Size:        112     431
 
DraI TTT'AAA

Cuts at:      0     509     543 
   Size:        509      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     377     543 
   Size:         41     336     166

  Fragments arranged by size: 

                 336     166      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0     226     543 
   Size:        226     317
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     314     346     543 
   Size:        314      32     197

  Fragments arranged by size: 

                 314     197      32
 
HincII GTy'rAC

Cuts at:      0     474     543 
   Size:        474      69
 
HindIII A'AGCT_T

Cuts at:      0     364     543 
   Size:        364     179
 
MfeI C'AATT_G

Cuts at:      0      82     543 
   Size:         82     461
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     262     414     543 
   Size:         59     203     152     129

  Fragments arranged by size: 

                 203     152     129      59
 
MslI CAynn'nnrTG

Cuts at:      0     445     543 
   Size:        445      98
 
NcoI C'CATG_G

Cuts at:      0     112     543 
   Size:        112     431
 
NdeI CA'TA_TG

Cuts at:      0     527     543 
   Size:        527      16
 
NspI r_CATG'y

Cuts at:      0     424     543 
   Size:        424     119
 
PfoI T'CCnGG_A

Cuts at:      0     354     543 
   Size:        354     189
 
PstI C_TGCA'G

Cuts at:      0     381     456     543 
   Size:        381      75      87

  Fragments arranged by size: 

                 381      87      75
 
SfcI C'TryA_G

Cuts at:      0     377     452     543 
   Size:        377      75      91

  Fragments arranged by size: 

                 377      91      75
 
SphI G_CATG'C

Cuts at:      0     424     543 
   Size:        424     119
 
StyI C'CwwG_G

Cuts at:      0     112     543 
   Size:        112     431
 
SwaI ATTT'AAAT

Cuts at:      0     509     543 
   Size:        509      34
 
TatI w'GTAC_w

Cuts at:      0     312     543 
   Size:        312     231
 

 Enzymes that do cut:

     AcuI     ApoI     BaeI     BbsI     BlpI     BplI     BpmI    BspHI
    BspMI    BstYI     BtgI     DraI  Eco57MI EcoO109I    Hin4I   HincII
  HindIII     MfeI     MmeI     MslI     NcoI     NdeI     NspI     PfoI
     PstI     SfcI     SphI     StyI     SwaI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MluI     MscI   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SrfI     SspI     StuI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI