Restriction map for
pFN21AE6372
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AcsI
AflII AflIII AgeI Alw44I AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BspDI BspLU11I BspMI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HindIII KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MscI MslI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
TfiI Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 69 TCCGGA T^CCGGA
AclI 502 AACGTT AA^CGTT
AcyI 1003 GGCGTC G[GA]^CG[CT]C
AhdI 408 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 779 AGCT AG^CT
AlwNI 971 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 668 AGCGCT AGC^GCT
AspEI 408 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 282 GGATCC G^GATCC
BanI 141 GGTGCC G^G[CT][GA]CC
BanII 26 GGGCTC G[GA]GC[CT]^C
BbsI 575 GTCTTC GAAGAC(2/6)
BciVI 715 GGATAC GTATCC(6/5)
BpuAI 575 GTCTTC GAAGAC(2/6)
BsaHI 1003 GGCGTC G[GA]^CG[CT]C
BseAI 69 TCCGGA T^CCGGA
BsgI 979 GTGCAG GTGCAG(16/14)
Bsp1407I 392 TGTACA T^GTACA
BspEI 69 TCCGGA T^CCGGA
BspHI 263 TCATGA T^CATGA
BsrBI 439 GAGCGG CCGCTC(-3/-3)
BsrGI 392 TGTACA T^GTACA
BsrI 724 ACTGG ACTGG(1/-1)
BstAPI 249 GCAGAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 655 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 669 GCGC GCG^C
DraII 701 AGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 408 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 668 AGCGCT AGC^GCT
EcoO109I 701 AGGACCC [GA]G^G[ACGT]CC[CT]
FauI 441 GCGGG CCCGC(4/6)
HaeII 668 AGCGCT [GA]GCGC^[CT]
HgaI 1004 GCGTC GACGC(5/10)
HhaI 669 GCGC GCG^C
Hin1I 1003 GGCGTC G[GA]^CG[CT]C
HinP1I 669 GCGC G^CGC
HincII 913 GTTAAC GT[CT]^[GA]AC
HindII 913 GTTAAC GT[CT]^[GA]AC
HpaI 913 GTTAAC GTT^AAC
MroI 69 TCCGGA T^CCGGA
NspI 389 GCATGT [GA]CATG^[CT]
PflMI 706 CCAACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 701 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 502 AACGTT AA^CGTT
RcaI 263 TCATGA T^CATGA
ScaI 614 AGTACT AGT^ACT
SfaNI 737 GCATC GCATC(5/9)
SfcI 54 CTACAG C^T[GA][CT]AG
SmaI 606 CCCGGG CCC^GGG
SspBI 392 TGTACA T^GTACA
StuI 653 AGGCCT AGG^CCT
TaqI 62 TCGA T^CGA
Tsp45I 133 GTGAC ^GT[GC]AC
TthHB8I 62 TCGA T^CGA
Van91I 706 CCAACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 606 CCCGGG C^CCGGG
XmaI 606 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 879 CCGC CCGC(-3/-1)
441 GCGG
AfaI 393 GTAC GT^AC
615 GTAC
AvaI 128 CTCGGG C^[CT]CG[GA]G
606 CCCGGG
AvaII 17 GGTCC G^G[AT]CC
702 GGACC
BbvI 525 GCAGC GCAGC(8/12)
370 GCTGC
BclI 377 TGATCA T^GATCA
464 TGATCA
BcnI 606 CCCGG CC^[GC]GG
607 CCGGG
BglI 361 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
542 GCCTAAATGGC
BmyI 26 GGGCTC G[AGT]GC[ACT]^C
142 GTGCCC
BsmFI 416 GTCCC GGGAC(10/14)
602 GTCCC
BsoBI 128 CTCGGG C^[CT]CG[GA]G
606 CCCGGG
Bsp1286I 26 GGGCTC G[AGT]GC[ACT]^C
142 GTGCCC
BstYI 282 GGATCC [GA]^GATC[CT]
786 AGATCC
DdeI 93 CTTAG C^T[ACGT]AG
776 CTGAG
EcoT14I 365 CCTTGG C^C[AT][AT]GG
929 CCTTGG
FbaI 377 TGATCA T^GATCA
464 TGATCA
Fnu4HI 370 GCTGC GC^[ACGT]GC
525 GCAGC
HaeIII 306 GGCC GG^CC
654 GGCC
ItaI 370 GCTGC GC^[ACGT]GC
525 GCAGC
MaeIII 133 GTGAC ^GT[ACGT]AC
848 GTTAC
MflI 282 GGATCC [GA]^GATC[CT]
786 AGATCC
NciI 606 CCCGG CC^[GC]GG
607 CCGGG
NlaIII 264 CATG CATG^
390 CATG
RsaI 393 GTAC GT^AC
615 GTAC
StyI 365 CCTTGG C^C[AT][AT]GG
929 CCTTGG
TseI 370 GCTGC G^C[AT]GC
525 GCAGC
Tsp509I 254 AATT ^AATT
662 AATT
TspRI 971 CAGTG CA[GC]TG[ACGT][ACGT]^
982 CAGTG
XhoII 282 GGATCC [GA]^GATC[CT]
786 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AlwI BpmI BsaJI BsaWI BseRI
BsiYI BslI BsrDI BssKI BstF5I
BstNI Cfr13I DpnII EarI EcoRII
FokI HapII HinfI HpaII HphI
Ksp632I MaeII MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
NlaIV PleI Sau3AI Sau96I ScrFI
Tru9I