(Circular) (Six-base) MAPSORT of: pFN21AE6372.seq Check: 7559 from: 1 to: 5776 pFN21AE6372 5776 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3119 1937 Size: 1182 4594 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 2508 3903 4276 1563 Size: 945 1395 373 3063 Fragments arranged by size: 3063 1395 945 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3017 3839 5223 5491 1632 Size: 1385 822 1384 268 1917 Fragments arranged by size: 1917 1385 1384 822 268 AfeI AGC'GCT Cuts at: 2675 2675 Size: 5776 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 4675 5652 1203 Size: 3472 977 1327 Fragments arranged by size: 3472 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 2418 4505 2418 Size: 2087 3689 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AlwNI CAG_nnn'CTG Cuts at: 2981 3519 5091 2981 Size: 538 1572 3666 Fragments arranged by size: 3666 1572 538 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 3085 3311 3463 5572 1493 Size: 1592 226 152 2109 1697 Fragments arranged by size: 2109 1697 1592 226 152 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 2133 2611 3101 1870 Size: 73 190 478 490 4545 Fragments arranged by size: 4545 490 478 190 73 BamHI G'GATC_C Cuts at: 2287 3106 3473 2287 Size: 819 367 4590 Fragments arranged by size: 4590 819 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2146 3097 4552 618 Size: 325 217 627 359 951 1455 1842 Fragments arranged by size: 1842 1455 951 627 359 325 217 BanII G_rGCy'C Cuts at: 1391 2035 3095 1391 Size: 644 1060 4072 Fragments arranged by size: 4072 1060 644 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2573 928 Size: 971 674 4131 Fragments arranged by size: 4131 971 674 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2714 3668 4878 2714 Size: 954 1210 3612 Fragments arranged by size: 3612 1210 954 BclI T'GATC_A Cuts at: 1548 2382 2469 1548 Size: 834 87 4855 Fragments arranged by size: 4855 834 87 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2372 2553 4387 Size: 107 122 71 631 605 700 181 1834 Cuts at: 4387 136 Size: 1525 Fragments arranged by size: 1834 1525 700 631 605 181 122 107 71 BglII A'GATC_T Cuts at: 5771 5771 Size: 5776 BlpI GC'TnA_GC Cuts at: 3193 3193 Size: 5776 Bme1580I G_kGCm'C Cuts at: 1790 2151 3837 4993 1790 Size: 361 1686 1156 2573 Fragments arranged by size: 2573 1686 1156 361 BmgBI CAC'GTC Cuts at: 1318 2959 1318 Size: 1641 4135 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3507 4465 442 Size: 416 53 83 399 24 2090 958 1753 Fragments arranged by size: 2090 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2481 2546 2723 4436 1239 Size: 1242 65 177 1713 2579 Fragments arranged by size: 2579 1713 1242 177 65 Bpu10I CC'TnA_GC Cuts at: 2781 2781 Size: 5776 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3901 4766 5064 3901 Size: 865 298 4613 Fragments arranged by size: 4613 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4439 882 Size: 617 2940 2219 Fragments arranged by size: 2940 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3567 493 Size: 711 2363 2702 Fragments arranged by size: 2702 2363 711 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3009 3583 3965 4600 Size: 53 83 186 936 1476 574 382 635 Cuts at: 4600 275 Size: 1451 Fragments arranged by size: 1476 1451 936 635 574 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2019 2074 2760 4209 4607 4881 5028 5439 2019 Size: 55 686 1449 398 274 147 411 2356 Fragments arranged by size: 2356 1449 686 411 398 274 147 55 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2781 2811 1178 Size: 30 165 30 1378 30 4143 Fragments arranged by size: 4143 1378 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2174 2177 2189 2192 2204 2213 2225 Size: 940 48 3 12 3 12 9 12 Cuts at: 2225 1186 Size: 4737 Fragments arranged by size: 4737 940 48 12 12 12 9 3 3 BseYI C'CCAG_C Cuts at: 4979 5475 4979 Size: 496 5280 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3005 1163 Size: 573 1269 3934 Fragments arranged by size: 3934 1269 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3837 3922 4993 3837 Size: 85 1071 4620 Fragments arranged by size: 4620 1071 85 BsmI GAATG_Cn' Cuts at: 3287 3380 3287 Size: 93 5683 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2151 3095 3837 3922 4993 1391 Size: 399 245 116 944 742 85 1071 2174 Fragments arranged by size: 2174 1071 944 742 399 245 116 85 BspEI T'CCGG_A Cuts at: 2074 5439 2074 Size: 3365 2411 BspHI T'CATG_A Cuts at: 2268 3665 5395 2268 Size: 1397 1730 2649 Fragments arranged by size: 2649 1730 1397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3131 844 Size: 2287 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2446 3663 1394 Size: 429 623 1217 3507 Fragments arranged by size: 3507 1217 623 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2248 2644 2949 4271 4445 65 Size: 1183 1000 396 305 1322 174 1396 Fragments arranged by size: 1396 1322 1183 1000 396 305 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4420 4607 1700 Size: 110 2610 187 2869 Fragments arranged by size: 2869 2610 187 110 BsrGI T'GTAC_A Cuts at: 96 2397 96 Size: 2301 3475 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 3836 4848 3836 Size: 1012 4764 BstAPI GCAn_nnn'nTGC Cuts at: 794 2260 794 Size: 1466 4310 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5776 BstYI r'GATC_y Cuts at: 1972 2287 2791 3106 3473 3858 3875 5327 5413 Size: 315 504 315 367 385 17 1452 86 Cuts at: 5413 5425 5771 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 504 385 367 346 315 315 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5224 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 2982 2993 3292 4086 4106 2982 Size: 11 299 794 20 4652 Fragments arranged by size: 4652 794 299 20 11 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4783 809 Size: 507 3467 1802 Fragments arranged by size: 3467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3223 4112 5741 Size: 54 1425 50 275 18 1393 889 1629 Cuts at: 5741 8 Size: 43 Fragments arranged by size: 1629 1425 1393 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2103 2121 2166 2181 2190 2211 3706 1353 Size: 750 18 45 15 9 21 1495 3423 Fragments arranged by size: 3423 1495 750 45 21 18 15 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4363 4737 4883 387 Size: 1064 138 2774 374 146 1280 Fragments arranged by size: 2774 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2481 2546 2723 3017 3839 4436 5223 Size: 393 849 65 177 294 822 597 787 Cuts at: 5223 5491 1239 Size: 268 1524 Fragments arranged by size: 1524 849 822 787 597 393 294 268 177 65 EcoO109I rG'GnC_Cy Cuts at: 2707 2707 Size: 5776 EcoRI G'AATT_C Cuts at: 3085 3463 3085 Size: 378 5398 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2695 2695 Size: 5776 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 2677 4923 1678 Size: 999 2246 2531 Fragments arranged by size: 2531 2246 999 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2404 2436 2454 2486 2828 Size: 32 135 32 832 32 18 32 342 Cuts at: 2828 2860 4417 4449 4491 4523 1373 Size: 32 1557 32 42 32 2626 Fragments arranged by size: 2626 1557 832 342 135 42 32 32 32 32 32 32 32 18 HincII GTy'rAC Cuts at: 669 1657 1938 2920 3120 3366 669 Size: 988 281 982 200 246 3079 Fragments arranged by size: 3079 988 982 281 246 200 HindIII A'AGCT_T Cuts at: 748 748 Size: 5776 HpaI GTT'AAC Cuts at: 2920 3366 2920 Size: 446 5330 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2309 4890 5074 1305 Size: 1004 2581 184 2007 Fragments arranged by size: 2581 2007 1004 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3954 Fragments arranged by size: 3954 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3734 4093 4252 518 Size: 648 2568 359 159 2042 Fragments arranged by size: 2568 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3192 3869 5017 5262 1606 Size: 1586 677 1148 245 2120 Fragments arranged by size: 2120 1586 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5224 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 2398 3134 4679 1132 Size: 97 1169 736 1545 2229 Fragments arranged by size: 2229 1545 1169 736 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 2717 1108 Size: 1609 4167 PfoI T'CCnGG_A Cuts at: 2458 2458 Size: 5776 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2707 2707 Size: 5776 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 2571 3346 2571 Size: 775 5001 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2078 2110 2078 Size: 32 5744 PstI C_TGCA'G Cuts at: 830 3128 830 Size: 2298 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 2621 4024 5736 2621 Size: 1403 1712 2661 Fragments arranged by size: 2661 1712 1403 SfcI C'TryA_G Cuts at: 826 1045 2059 3124 4259 4940 5131 826 Size: 219 1014 1065 1135 681 191 1471 Fragments arranged by size: 1471 1135 1065 1014 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 2613 3103 1872 Size: 741 490 4545 Fragments arranged by size: 4545 741 490 SmlI C'TyrA_G Cuts at: 820 1017 1943 3880 4781 5043 820 Size: 197 926 1937 901 262 1553 Fragments arranged by size: 1937 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 2660 1517 Size: 1143 4633 StyI C'CwwG_G Cuts at: 513 1065 2370 2934 3215 513 Size: 552 1305 564 281 3074 Fragments arranged by size: 3074 1305 564 552 281 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3803 3820 3973 4158 4603 325 Size: 1255 2223 17 153 185 445 1498 Fragments arranged by size: 2223 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2397 2619 4022 Size: 274 80 33 51 1443 420 222 1403 Cuts at: 4022 5734 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1403 420 274 222 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2781 2813 5736 5768 2781 Size: 32 2923 32 2789 Fragments arranged by size: 2923 2789 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 2611 3101 1870 Size: 741 490 4545 Fragments arranged by size: 4545 741 490 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI