################################## # NO CUT ENZYMES ( 221 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HgaI Hin1I HincII HindII HindIII HpaI HphI ItaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboII MfeI MflI MluI MluNI MnlI MroI MscI MseI MspA1I MunI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I TseI Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AfaI 72 GTAC GT^AC AhdI 23 GACTCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 23 GACTCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BcnI 19 CCCGG CC^[GC]GG BsaJI 18 CCCCGG C^C[ACGT][ACGT]GG BstF5I 75 CATCC GGATG(2/0) BstNI 55 CCAGG CC^[AT]GG CfoI 156 GCGC GCG^C DpnII 172 GATC ^GATC Eam1105I 23 GACTCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRII 55 CCAGG ^CC[AT]GG FokI 75 CATCC GGATG(9/13) HapII 20 CCGG C^CGG HhaI 156 GCGC GCG^C HinP1I 156 GCGC G^CGC HpaII 20 CCGG C^CGG MaeIII 176 GTTAC ^GT[ACGT]AC MboI 172 GATC ^GATC MslI 33 CATGGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 20 CCGG C^CGG MvaI 55 CCAGG CC^[AT]GG NciI 19 CCCGG CC^[GC]GG NdeII 172 GATC ^GATC NlaIII 33 CATG CATG^ RsaI 72 GTAC GT^AC Sau3AI 172 GATC ^GATC SfaNI 158 GCATC GCATC(5/9) TaqI 8 TCGA T^CGA TspRI 130 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 8 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 6 enzymes) # ################################### BpmI 132 CTGGAG CTGGAG(16/14) 164 CTGGAG BsrI 60 ACTGG ACTGG(1/-1) 131 ACTGG BssKI 19 CCCGG ^CC[ACGT]GG 55 CCAGG PleI 23 GACTC GAGTC(4/5) 135 GAGTC ScrFI 19 CCCGG CC^[ACGT]GG 55 CCAGG TfiI 12 GATTC G^A[AT]TC 51 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 1 enzymes) # ######################################### HinfI