(Circular) (Six-base) MAPSORT of: pFN21AE2309.seq Check: 6423 from: 1 to: 8476 pFN21AE2309 8476 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3258 3653 4273 3258 Size: 395 620 7461 Fragments arranged by size: 7461 620 395 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6286 278 Size: 53 83 186 936 4750 2468 Fragments arranged by size: 4750 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5148 5819 1937 Size: 3211 671 4594 Fragments arranged by size: 4594 3211 671 Acc65I G'GTAC_C Cuts at: 2493 5797 2493 Size: 3304 5172 AclI AA'CG_TT Cuts at: 1563 6603 6976 1563 Size: 5040 373 3063 Fragments arranged by size: 5040 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3399 3854 4563 4617 6539 7923 8191 1632 Size: 1767 455 709 54 1922 1384 268 1917 Fragments arranged by size: 1922 1917 1767 1384 709 455 268 54 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8279 AflIII A'CryG_T Cuts at: 1203 4341 7375 8352 1203 Size: 3138 3034 977 1327 Fragments arranged by size: 3138 3034 1327 977 AgeI A'CCGG_T Cuts at: 7307 7307 Size: 8476 AhdI GACnn_n'nnGTC Cuts at: 2203 7205 2203 Size: 5002 3474 AleI CACnn'nnGTG Cuts at: 1166 2816 5717 1166 Size: 1650 2901 3925 Fragments arranged by size: 3925 2901 1650 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8476 AlwNI CAG_nnn'CTG Cuts at: 2842 5462 6219 7791 2842 Size: 2620 757 1572 3527 Fragments arranged by size: 3527 2620 1572 757 ApaLI G'TGCA_C Cuts at: 6533 7689 6533 Size: 1156 7320 ApoI r'AATT_y Cuts at: 1493 5785 6011 6163 8272 1493 Size: 4292 226 152 2109 1697 Fragments arranged by size: 4292 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7030 160 Size: 872 5998 1606 Fragments arranged by size: 5998 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8476 AvaI C'yCGr_G Cuts at: 1870 1943 2113 2303 2600 3101 3553 3591 4053 Size: 73 170 190 297 501 452 38 462 Cuts at: 4053 5327 5434 5801 1870 Size: 1274 107 367 4545 Fragments arranged by size: 4545 1274 501 462 452 367 297 190 170 107 73 38 BamHI G'GATC_C Cuts at: 3587 3800 5806 6173 3587 Size: 213 2006 367 5890 Fragments arranged by size: 5890 2006 367 213 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2408 2493 3047 3081 3094 Size: 325 217 627 621 85 554 34 13 Cuts at: 3094 3336 3656 5668 5797 7252 618 Size: 242 320 2012 129 1455 1842 Fragments arranged by size: 2012 1842 1455 627 621 554 325 320 242 217 129 85 34 13 BanII G_rGCy'C Cuts at: 1391 2925 3019 3367 3488 3506 3524 3608 3623 Size: 1534 94 348 121 18 18 84 15 Cuts at: 3623 4259 4424 4457 4647 5539 5572 5795 1391 Size: 636 165 33 190 892 33 223 4072 Fragments arranged by size: 4072 1534 892 636 348 223 190 165 121 94 84 33 33 18 18 15 BbeI G_GCGC'C Cuts at: 2412 3051 2412 Size: 639 7837 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2050 3180 3247 3366 5257 928 Size: 971 151 1130 67 119 1891 4147 Fragments arranged by size: 4147 1891 1130 971 151 119 67 BbvCI CC'TCA_GC Cuts at: 5350 5393 5350 Size: 43 8433 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3025 3059 6667 6701 1903 Size: 34 1088 34 3608 34 3678 Fragments arranged by size: 3678 3608 1088 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6368 7578 6368 Size: 1210 7266 BclI T'GATC_A Cuts at: 1548 2445 1548 Size: 897 7579 BfrBI ATG'CAT Cuts at: 2965 2965 Size: 8476 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2381 2622 2663 Size: 107 122 71 631 605 709 241 41 Cuts at: 2663 3259 4114 7087 136 Size: 596 855 2973 1525 Fragments arranged by size: 2973 1525 855 709 631 605 596 241 122 107 71 41 BglII A'GATC_T Cuts at: 4933 8471 4933 Size: 3538 4938 BlpI GC'TnA_GC Cuts at: 2585 2753 3310 5893 2585 Size: 168 557 2583 5168 Fragments arranged by size: 5168 2583 557 168 Bme1580I G_kGCm'C Cuts at: 1790 2280 2742 3659 3672 6537 7693 1790 Size: 490 462 917 13 2865 1156 2573 Fragments arranged by size: 2865 2573 1156 917 490 462 13 BmgBI CAC'GTC Cuts at: 1318 3329 5741 1318 Size: 2011 2412 4053 Fragments arranged by size: 4053 2412 2011 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4558 6207 7165 Size: 416 53 83 399 24 3141 1649 958 Cuts at: 7165 442 Size: 1753 Fragments arranged by size: 3141 1753 1649 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8476 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3134 4327 3134 Size: 1193 7283 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3214 3523 3717 3753 3945 4129 4377 4626 Size: 1975 309 194 36 192 184 248 249 Cuts at: 4626 5586 7136 1239 Size: 960 1550 2579 Fragments arranged by size: 2579 1975 1550 960 309 249 248 194 192 184 36 Bpu10I CC'TnA_GC Cuts at: 2843 3487 3505 3622 3712 3748 3784 5350 5393 Size: 644 18 117 90 36 36 1566 43 Cuts at: 5393 2843 Size: 5926 Fragments arranged by size: 5926 1566 644 117 90 43 36 36 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4803 6601 7466 7764 4803 Size: 1798 865 298 5515 Fragments arranged by size: 5515 1798 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3685 4660 5328 7139 882 Size: 617 2186 975 668 1811 2219 Fragments arranged by size: 2219 2186 1811 975 668 617 BsaAI yAC'GTr Cuts at: 493 1204 4105 4955 5719 6267 493 Size: 711 2901 850 764 548 2702 Fragments arranged by size: 2901 2702 850 764 711 548 BsaBI GATnn'nnATC Cuts at: 8470 8470 Size: 8476 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2316 2409 3048 5254 Size: 53 83 186 936 783 93 639 2206 Cuts at: 5254 6283 6665 7300 275 Size: 1029 382 635 1451 Fragments arranged by size: 2206 1451 1029 936 783 639 635 382 186 93 83 53 BsaWI w'CCGG_w Cuts at: 5641 6909 7307 7581 7728 8139 5641 Size: 1268 398 274 147 411 5978 Fragments arranged by size: 5978 1268 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2780 2810 4792 4822 1178 Size: 30 165 30 1377 30 1982 30 4832 Fragments arranged by size: 4832 1982 1377 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2228 2801 3894 3897 4002 4158 1186 Size: 1042 573 1093 3 105 156 5504 Fragments arranged by size: 5504 1093 1042 573 156 105 3 BseYI C'CCAG_C Cuts at: 2320 2734 3209 3317 3322 5189 7679 8175 2320 Size: 414 475 108 5 1867 2490 496 2621 Fragments arranged by size: 2621 2490 1867 496 475 414 108 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2076 3021 3230 4068 1163 Size: 573 340 945 209 838 5571 Fragments arranged by size: 5571 945 838 573 340 209 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2127 2400 2658 5926 6687 7715 Size: 275 186 126 273 258 3268 761 1028 Cuts at: 7715 1540 Size: 2301 Fragments arranged by size: 3268 2301 1028 761 275 273 258 186 126 BsiHKAI G_wGCw'C Cuts at: 2298 2925 3367 3641 4424 4457 6537 6622 7693 Size: 627 442 274 783 33 2080 85 1071 Cuts at: 7693 2298 Size: 3081 Fragments arranged by size: 3081 2080 1071 783 627 442 274 85 33 BsmI GAATG_Cn' Cuts at: 4007 5987 6080 4007 Size: 1980 93 6403 Fragments arranged by size: 6403 1980 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2280 2298 2742 2925 3019 3367 3488 Size: 399 490 18 444 183 94 348 121 Cuts at: 3488 3506 3524 3608 3623 3641 3659 3672 4259 Size: 18 18 84 15 18 18 13 587 Cuts at: 4259 4424 4457 4647 5539 5572 5795 6537 6622 Size: 165 33 190 892 33 223 742 85 Cuts at: 6622 7693 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 892 742 587 490 444 399 348 223 190 183 165 121 94 85 84 33 33 18 18 18 18 18 15 13 BspEI T'CCGG_A Cuts at: 5641 8139 5641 Size: 2498 5978 BspHI T'CATG_A Cuts at: 4639 6365 8095 4639 Size: 1726 1730 5020 Fragments arranged by size: 5020 1730 1726 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2229 2265 2701 2886 2923 3255 3258 3653 Size: 1385 36 436 185 37 332 3 395 Cuts at: 3653 4273 5831 844 Size: 620 1558 3489 Fragments arranged by size: 3489 1558 1385 620 436 395 332 185 37 36 3 BsrBI CCG'CTC Cuts at: 1394 1823 2425 2757 3100 3278 5639 6363 1394 Size: 429 602 332 343 178 2361 724 3507 Fragments arranged by size: 3507 2361 724 602 429 343 332 178 BsrDI GCAATG_nn' Cuts at: 65 1248 2505 5032 5082 6971 7145 65 Size: 1183 1257 2527 50 1889 174 1396 Fragments arranged by size: 2527 1889 1396 1257 1183 174 50 BsrFI r'CCGG_y Cuts at: 1700 1810 2653 3289 4361 5549 7120 7307 1700 Size: 110 843 636 1072 1188 1571 187 2869 Fragments arranged by size: 2869 1571 1188 1072 843 636 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8476 BssHII G'CGCG_C Cuts at: 1925 2634 1925 Size: 709 7767 BssSI C'ACGA_G Cuts at: 6536 7548 6536 Size: 1012 7464 BstAPI GCAn_nnn'nTGC Cuts at: 794 5591 794 Size: 4797 3679 BstBI TT'CG_AA Cuts at: 7280 7280 Size: 8476 BstEII G'GTnAC_C Cuts at: 1184 2092 1184 Size: 908 7568 BstXI CCAn_nnnn'nTGG Cuts at: 2734 3695 3731 4836 2734 Size: 961 36 1105 6374 Fragments arranged by size: 6374 1105 961 36 BstYI r'GATC_y Cuts at: 1972 3587 3800 3842 4035 4933 5806 6173 6558 Size: 1615 213 42 193 898 873 367 385 Cuts at: 6558 6575 8027 8113 8125 8471 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1615 1452 898 873 385 367 346 213 193 86 42 17 12 Bsu36I CC'TnA_GG Cuts at: 3152 4392 3152 Size: 1240 7236 BtgI C'CryG_G Cuts at: 513 1065 2004 2175 2376 2435 3742 5088 513 Size: 552 939 171 201 59 1307 1346 3901 Fragments arranged by size: 3901 1346 1307 939 552 201 171 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2552 487 Size: 683 56 1326 6411 Fragments arranged by size: 6411 1326 683 56 BtsI GCAGTG_nn' Cuts at: 2094 5992 6786 6806 2094 Size: 3898 794 20 3764 Fragments arranged by size: 3898 3764 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8476 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4117 4152 529 Size: 35 3553 35 4853 Fragments arranged by size: 4853 3553 35 35 DraI TTT'AAA Cuts at: 5780 5780 Size: 8476 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2146 2225 2789 4535 7483 809 Size: 507 830 79 564 1746 2948 1802 Fragments arranged by size: 2948 1802 1746 830 564 507 79 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2178 2397 2544 Size: 54 1425 50 275 18 348 219 147 Cuts at: 2544 2655 2820 3291 3496 3514 3529 3613 3649 Size: 111 165 471 205 18 15 84 36 Cuts at: 3649 3685 3721 3739 4125 4988 5923 6812 8441 Size: 36 36 18 386 863 935 889 1629 Cuts at: 8441 8 Size: 43 Fragments arranged by size: 1629 1425 935 889 863 471 386 348 275 219 205 165 147 111 84 54 50 43 36 36 36 18 18 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2397 2655 5923 1537 Size: 275 585 258 3268 4090 Fragments arranged by size: 4090 3268 585 275 258 EarI CTCTTCn'nnn_ Cuts at: 1353 2205 4408 5034 5232 6406 1353 Size: 852 2203 626 198 1174 3423 Fragments arranged by size: 3423 2203 1174 852 626 198 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2696 5464 7063 7437 7583 387 Size: 1064 138 1107 2768 1599 374 146 1280 Fragments arranged by size: 2768 1599 1280 1107 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2923 3365 4422 4455 2923 Size: 442 1057 33 6944 Fragments arranged by size: 6944 1057 442 33 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3214 3399 3523 3717 3753 3854 3945 Size: 393 1582 185 124 194 36 101 91 Cuts at: 3945 4129 4377 4563 4617 4626 5586 6539 7136 Size: 184 248 186 54 9 960 953 597 Cuts at: 7136 7923 8191 1239 Size: 787 268 1524 Fragments arranged by size: 1582 1524 960 953 787 597 393 268 248 194 186 185 184 124 101 91 54 36 9 EcoO109I rG'GnC_Cy Cuts at: 2218 3126 3728 4058 4522 4552 2218 Size: 908 602 330 464 30 6142 Fragments arranged by size: 6142 908 602 464 330 30 EcoRI G'AATT_C Cuts at: 5785 6163 5785 Size: 378 8098 FspI TGC'GCA Cuts at: 6982 6982 Size: 8476 HaeII r_GCGC'y Cuts at: 1678 2287 2412 2614 3051 3199 4198 7623 1678 Size: 609 125 202 437 148 999 3425 2531 Fragments arranged by size: 3425 2531 999 609 437 202 148 125 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2189 2221 2540 2572 2836 Size: 32 135 32 617 32 319 32 264 Cuts at: 2836 2868 4601 4633 4689 4721 5286 5318 5462 Size: 32 1733 32 56 32 565 32 144 Cuts at: 5462 5494 7117 7149 7191 7223 1373 Size: 32 1623 32 42 32 2626 Fragments arranged by size: 2626 1733 1623 617 565 319 264 144 135 56 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3060 3982 4771 5149 5820 6066 Size: 988 281 1122 922 789 378 671 246 Cuts at: 6066 669 Size: 3079 Fragments arranged by size: 3079 1122 988 922 789 671 378 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8476 HpaI GTT'AAC Cuts at: 3060 6066 3060 Size: 3006 5470 KasI G'GCGC_C Cuts at: 2408 3047 2408 Size: 639 7837 KpnI G_GTAC'C Cuts at: 2497 5801 2497 Size: 3304 5172 MfeI C'AATT_G Cuts at: 3478 6075 3478 Size: 2597 5879 MluI A'CGCG_T Cuts at: 8352 8352 Size: 8476 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2538 4397 5339 7590 7774 1305 Size: 1233 1859 942 2251 184 2007 Fragments arranged by size: 2251 2007 1859 1233 942 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2822 3516 3531 3615 3651 Size: 54 1425 343 990 694 15 84 36 Cuts at: 3651 3687 3723 3741 4127 4990 10 Size: 36 36 18 386 863 3496 Fragments arranged by size: 3496 1425 990 863 694 386 343 84 54 36 36 36 18 15 MslI CAynn'nnrTG Cuts at: 518 1166 2816 3470 4238 4346 5717 6434 6793 Size: 648 1650 654 768 108 1371 717 359 Cuts at: 6793 6952 518 Size: 159 2042 Fragments arranged by size: 2042 1650 1371 768 717 654 648 359 159 108 MspA1I CmG'CkG Cuts at: 1606 2063 2072 2378 2393 2437 2619 2675 3021 Size: 457 9 306 15 44 182 56 346 Cuts at: 3021 3923 5300 5459 5487 5627 5633 5892 6569 Size: 902 1377 159 28 140 6 259 677 Cuts at: 6569 7717 7962 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1377 1148 902 677 457 346 306 259 245 182 159 140 56 44 28 15 9 6 NaeI GCC'GGC Cuts at: 2655 3291 2655 Size: 636 7840 NarI GG'CG_CC Cuts at: 2409 3048 2409 Size: 639 7837 NcoI C'CATG_G Cuts at: 513 1065 2004 3742 5088 513 Size: 552 939 1738 1346 3901 Fragments arranged by size: 3901 1738 1346 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8476 NgoMIV G'CCGG_C Cuts at: 2653 3289 2653 Size: 636 7840 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8476 NotI GC'GGCC_GC Cuts at: 2397 5923 2397 Size: 3526 4950 NruI TCG'CGA Cuts at: 2028 2537 2028 Size: 509 7967 NsiI A_TGCA'T Cuts at: 2967 2967 Size: 8476 NspI r_CATG'y Cuts at: 1132 1229 2965 3469 4345 5834 7379 1132 Size: 97 1736 504 876 1489 1545 2229 Fragments arranged by size: 2229 1736 1545 1489 876 504 97 PasI CC'CwG_GG Cuts at: 1348 2727 3427 1348 Size: 1379 700 6397 Fragments arranged by size: 6397 1379 700 PciI A'CATG_T Cuts at: 4341 7375 4341 Size: 3034 5442 PflMI CCAn_nnn'nTGG Cuts at: 1108 3923 4416 1108 Size: 2815 493 5168 Fragments arranged by size: 5168 2815 493 PfoI T'CCnGG_A Cuts at: 2155 2914 4354 2155 Size: 759 1440 6277 Fragments arranged by size: 6277 1440 759 PmeI GTTT'AAAC Cuts at: 5780 5780 Size: 8476 PmlI CAC'GTG Cuts at: 4955 5719 4955 Size: 764 7712 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6545 6577 8058 8090 6545 Size: 32 1481 32 6931 Fragments arranged by size: 6931 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2218 3126 3728 4058 4552 2218 Size: 908 602 330 494 6142 Fragments arranged by size: 6142 908 602 494 330 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8476 PsiI TTA'TAA Cuts at: 6046 6046 Size: 8476 PspXI vC'TCGA_Gb Cuts at: 3101 4053 3101 Size: 952 7524 PstI C_TGCA'G Cuts at: 830 2389 3346 5232 5377 5632 5828 830 Size: 1559 957 1886 145 255 196 3478 Fragments arranged by size: 3478 1886 1559 957 255 196 145 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8476 PvuII CAG'CTG Cuts at: 3923 5459 5633 3923 Size: 1536 174 6766 Fragments arranged by size: 6766 1536 174 RsrII CG'GwC_CG Cuts at: 2912 2912 Size: 8476 SacI G_AGCT'C Cuts at: 2925 3367 4424 4457 2925 Size: 442 1057 33 6944 Fragments arranged by size: 6944 1057 442 33 SacII CC_GC'GG Cuts at: 2379 2438 2379 Size: 59 8417 SalI G'TCGA_C Cuts at: 1936 5147 5818 1936 Size: 3211 671 4594 Fragments arranged by size: 4594 3211 671 SanDI GG'GwC_CC Cuts at: 4552 4552 Size: 8476 SapI GCTCTTCn'nnn_ Cuts at: 4408 5232 4408 Size: 824 7652 SbfI CC_TGCA'GG Cuts at: 5828 5828 Size: 8476 ScaI AGT'ACT Cuts at: 6724 8436 6724 Size: 1712 6764 SfcI C'TryA_G Cuts at: 826 1045 2385 3031 3342 5016 5214 5228 5373 Size: 219 1340 646 311 1674 198 14 145 Cuts at: 5373 5628 5824 6959 7640 7831 826 Size: 255 196 1135 681 191 1471 Fragments arranged by size: 1674 1471 1340 1135 681 646 311 255 219 198 196 191 145 14 SfoI GGC'GCC Cuts at: 2410 3049 2410 Size: 639 7837 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8476 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8476 SmaI CCC'GGG Cuts at: 1872 5436 5803 1872 Size: 3564 367 4545 Fragments arranged by size: 4545 3564 367 SmlI C'TyrA_G Cuts at: 820 1017 1943 2303 2600 3101 4053 4818 5327 Size: 197 926 360 297 501 952 765 509 Cuts at: 5327 6580 7481 7743 820 Size: 1253 901 262 1553 Fragments arranged by size: 1553 1253 952 926 901 765 509 501 360 297 262 197 SnaBI TAC'GTA Cuts at: 493 6267 493 Size: 5774 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8476 SphI G_CATG'C Cuts at: 1132 5834 1132 Size: 4702 3774 SspI AAT'ATT Cuts at: 5 52 6400 5 Size: 47 6348 2081 Fragments arranged by size: 6348 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8476 StyI C'CwwG_G Cuts at: 513 1065 2004 3532 3742 5088 5915 513 Size: 552 939 1528 210 1346 827 3074 Fragments arranged by size: 3074 1528 1346 939 827 552 210 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2534 2900 3223 3331 6503 6520 6673 Size: 1255 954 366 323 108 3172 17 153 Cuts at: 6673 6858 7303 325 Size: 185 445 1498 Fragments arranged by size: 3172 1498 1255 954 445 366 323 185 153 108 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6722 8434 96 Size: 274 80 33 51 1443 4745 1712 138 Fragments arranged by size: 4745 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4190 4222 8436 8468 4190 Size: 32 4214 32 4198 Fragments arranged by size: 4214 4198 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2143 4766 1316 Size: 827 2623 5026 Fragments arranged by size: 5026 2623 827 XbaI T'CTAG_A Cuts at: 3641 5812 3641 Size: 2171 6305 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3217 3524 3749 3217 Size: 307 225 7944 Fragments arranged by size: 7944 307 225 XhoI C'TCGA_G Cuts at: 1943 2303 2600 3101 4053 5327 1943 Size: 360 297 501 952 1274 5092 Fragments arranged by size: 5092 1274 952 501 360 297 XmaI C'CCGG_G Cuts at: 1870 5434 5801 1870 Size: 3564 367 4545 Fragments arranged by size: 4545 3564 367 XmnI GAAnn'nnTTC Cuts at: 6605 6605 Size: 8476 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6284 276 Size: 53 83 186 936 4750 2468 Fragments arranged by size: 4750 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI PacI PspOMI PsrI SexAI SfiI SrfI SwaI