Restriction map for
pFN21AE2316
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccIII AciI AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI KpnI KspI MaeII
MamI MluNI MroI MscI MslI
MspI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 275 GTAGAC GT^[AC][GT]AC
AccII 789 CGCG CG^CG
AfaI 1087 GTAC GT^AC
AflIII 788 ACGCGT A^C[GA][CT]GT
AlwNI 212 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 201 GGGCCC GGGCC^C
Asp700I 1048 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 942 GGATCC G^GATCC
BanI 634 GGCACC G^G[CT][GA]CC
BfaI 55 CTAG C^TAG
BlpI 650 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 650 GCTCAGC GC^T[ACGT]AGC
BseRI 1153 GAGGAG GAGGAG(10/8)
BsmI 727 GCATTC GAATGC(1/-1)
BsrDI 901 CATTGC GCAATG(2/0)
BstUI 789 CGCG CG^CG
BstXI 1066 CCATCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 650 GCTCAGC GC^T[ACGT]AGC
DraII 201 GGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 868 GAAGAG CTCTTC(1/4)
EcoO109I 201 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 541 GATATC GAT^ATC
EcoT14I 497 CCTTGG C^C[AT][AT]GG
EcoT22I 725 ATGCAT ATGCA^T
HincII 859 GTTGAC GT[CT]^[GA]AC
HindII 859 GTTGAC GT[CT]^[GA]AC
Ksp632I 868 GAAGAG CTCTTC(1/4)
MaeI 55 CTAG C^TAG
MluI 788 ACGCGT A^CGCGT
MspA1I 584 CAGCTG C[AC]G^C[GT]G
MvnI 789 CGCG CG^CG
NsiI 725 ATGCAT ATGCA^T
PstI 907 CTGCAG CTGCA^G
PvuII 584 CAGCTG CAG^CTG
RsaI 1087 GTAC GT^AC
SacI 820 GAGCTC GAGCT^C
StuI 595 AGGCCT AGG^CCT
StyI 497 CCTTGG C^C[AT][AT]GG
XmnI 1048 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspHI 149 GTGCTC G[AT]GC[AT]^C
820 GAGCTC
AvaII 439 GGACC G^G[AT]CC
834 GGACC
BanII 201 GGGCCC G[GA]GC[CT]^C
820 GAGCTC
BbvI 102 GCAGC GCAGC(8/12)
669 GCTGC
BsaJI 497 CCTTGG C^C[ACGT][ACGT]GG
690 CCTGGG
BsiHKAI 149 GTGCTC G[AT]GC[AT]^C
820 GAGCTC
BsmFI 290 GGGAC GGGAC(10/14)
912 GTCCC
BstYI 89 GGATCT [GA]^GATC[CT]
942 GGATCC
Cac8I 255 GCTGGC GC[ACGT]^[ACGT]GC
905 GCCTGC
EcoRI 492 GAATTC G^AATTC
1126 GAATTC
Fnu4HI 102 GCAGC GC^[ACGT]GC
669 GCTGC
ItaI 102 GCAGC GC^[ACGT]GC
669 GCTGC
MfeI 188 CAATTG C^AATTG
422 CAATTG
MflI 89 GGATCT [GA]^GATC[CT]
942 GGATCC
MunI 188 CAATTG C^AATTG
422 CAATTG
MwoI 577 GCTGACACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
672 GCTCATTGTGC
PflMI 187 CCAATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
384 CCATCAAATGG
PleI 1135 GACTC GAGTC(4/5)
4 GAGTC
SmlI 260 CTCAAG C^T[CT][GA]AG
570 CTTGAG
TseI 102 GCAGC G^C[AT]GC
669 GCTGC
Van91I 187 CCAATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
384 CCATCAAATGG
XhoII 89 GGATCT [GA]^GATC[CT]
942 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI AluI AlwI ApoI BmyI
BpmI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EcoRII FokI HaeIII HinfI
HphI MaeIII MboI MboII MnlI
MseI MvaI NdeII NlaIV Sau3AI
Sau96I ScrFI SfaNI SfcI TfiI
Tru9I Tsp45I Tsp509I TspRI