(Circular) (Six-base) MAPSORT of: pFN21AE2316.seq Check: 6021 from: 1 to: 5899 pFN21AE2316 5899 bp With 182 enzymes: SgfI * January 11, 2011 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3709 278 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2281 3242 1937 Size: 344 961 4594 Fragments arranged by size: 4594 961 344 Acc65I G'GTAC_C Cuts at: 3220 3220 Size: 5899 AclI AA'CG_TT Cuts at: 1563 4026 4399 1563 Size: 2463 373 3063 Fragments arranged by size: 3063 2463 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2892 2937 3962 5346 5614 1632 Size: 1260 45 1025 1384 268 1917 Fragments arranged by size: 1917 1384 1260 1025 268 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5702 AflIII A'CryG_T Cuts at: 1203 2793 4798 5775 1203 Size: 1590 2005 977 1327 Fragments arranged by size: 2005 1590 1327 977 AgeI A'CCGG_T Cuts at: 4730 4730 Size: 5899 AhdI GACnn_n'nnGTC Cuts at: 4628 4628 Size: 5899 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5899 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5899 AlwNI CAG_nnn'CTG Cuts at: 2222 3642 5214 2222 Size: 1420 1572 2907 Fragments arranged by size: 2907 1572 1420 ApaI G_GGCC'C Cuts at: 2210 2210 Size: 5899 ApaLI G'TGCA_C Cuts at: 3956 5112 3956 Size: 1156 4743 ApoI r'AATT_y Cuts at: 1493 2183 2497 3024 3037 3131 3208 3434 3586 Size: 690 314 527 13 94 77 226 152 Cuts at: 3586 5695 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 690 527 314 226 152 94 77 13 AseI AT'TA_AT Cuts at: 160 1032 4453 160 Size: 872 3421 1606 Fragments arranged by size: 3421 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 AvaI C'yCGr_G Cuts at: 1870 1943 3224 1870 Size: 73 1281 4545 Fragments arranged by size: 4545 1281 73 BamHI G'GATC_C Cuts at: 2947 3229 3596 2947 Size: 282 367 5250 Fragments arranged by size: 5250 367 282 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2639 3220 4675 618 Size: 325 217 627 852 581 1455 1842 Fragments arranged by size: 1842 1455 852 627 581 325 217 BanII G_rGCy'C Cuts at: 1391 2210 2829 3218 1391 Size: 819 619 389 4072 Fragments arranged by size: 4072 819 619 389 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4928 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4090 4124 1903 Size: 34 2153 34 3678 Fragments arranged by size: 3678 2153 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3791 5001 3791 Size: 1210 4689 BclI T'GATC_A Cuts at: 1548 1548 Size: 5899 BfrBI ATG'CAT Cuts at: 2732 2732 Size: 5899 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4510 136 Size: 107 122 71 631 605 2838 1525 Fragments arranged by size: 2838 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5894 5894 Size: 5899 BlpI GC'TnA_GC Cuts at: 2656 3316 2656 Size: 660 5239 Bme1580I G_kGCm'C Cuts at: 1790 2210 2642 2688 3960 5116 1790 Size: 420 432 46 1272 1156 2573 Fragments arranged by size: 2573 1272 1156 432 420 46 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5899 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3132 3630 4588 Size: 416 53 83 399 24 1715 498 958 Cuts at: 4588 442 Size: 1753 Fragments arranged by size: 1753 1715 958 498 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5899 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2320 3081 3176 3191 4559 1239 Size: 1081 761 95 15 1368 2579 Fragments arranged by size: 2579 1368 1081 761 95 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2250 2596 4024 4889 5187 2250 Size: 346 1428 865 298 2962 Fragments arranged by size: 2962 1428 865 346 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4562 882 Size: 617 3063 2219 Fragments arranged by size: 3063 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3690 493 Size: 711 2486 2702 Fragments arranged by size: 2702 2486 711 BsaBI GATnn'nnATC Cuts at: 5893 5893 Size: 5899 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3706 4088 4723 275 Size: 53 83 186 936 2173 382 635 1451 Fragments arranged by size: 2173 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4332 4730 5004 5151 5562 4332 Size: 398 274 147 411 4669 Fragments arranged by size: 4669 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5674 Fragments arranged by size: 5674 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3173 1186 Size: 1987 3912 BseYI C'CCAG_C Cuts at: 2304 5102 5598 2304 Size: 2798 496 2605 Fragments arranged by size: 2798 2605 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5326 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3349 4110 5138 1540 Size: 275 186 1348 761 1028 2301 Fragments arranged by size: 2301 1348 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2158 2829 3960 4045 5116 2158 Size: 671 1131 85 1071 2941 Fragments arranged by size: 2941 1131 1071 671 85 BsmI GAATG_Cn' Cuts at: 2732 3410 3503 2732 Size: 678 93 5128 Fragments arranged by size: 5128 678 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2158 2210 2642 2688 2829 3218 3960 Size: 399 368 52 432 46 141 389 742 Cuts at: 3960 4045 5116 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 432 399 389 368 141 85 52 46 BspEI T'CCGG_A Cuts at: 5562 5562 Size: 5899 BspHI T'CATG_A Cuts at: 3788 5518 3788 Size: 1730 4169 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3254 844 Size: 2410 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3786 1394 Size: 429 1963 3507 Fragments arranged by size: 3507 1963 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2905 4394 4568 65 Size: 1183 1657 1489 174 1396 Fragments arranged by size: 1657 1489 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4543 4730 1700 Size: 110 2733 187 2869 Fragments arranged by size: 2869 2733 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5899 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5899 BssSI C'ACGA_G Cuts at: 3959 4971 3959 Size: 1012 4887 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5899 BstBI TT'CG_AA Cuts at: 4703 4703 Size: 5899 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5899 BstXI CCAn_nnnn'nTGG Cuts at: 3078 3078 Size: 5899 BstYI r'GATC_y Cuts at: 1972 2094 2947 3229 3596 3981 3998 5450 5536 Size: 122 853 282 367 385 17 1452 86 Cuts at: 5536 5548 5894 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 853 385 367 346 282 122 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4408 Fragments arranged by size: 4408 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5160 Fragments arranged by size: 5160 683 56 BtsI GCAGTG_nn' Cuts at: 3415 4209 4229 3415 Size: 794 20 5085 Fragments arranged by size: 5085 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5899 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5864 DraI TTT'AAA Cuts at: 3203 3203 Size: 5899 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4906 809 Size: 507 3590 1802 Fragments arranged by size: 3590 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3346 4235 5864 Size: 54 1425 50 275 18 1516 889 1629 Cuts at: 5864 8 Size: 43 Fragments arranged by size: 1629 1516 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3346 1537 Size: 275 1534 4090 Fragments arranged by size: 4090 1534 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2868 3829 1353 Size: 1515 961 3423 Fragments arranged by size: 3423 1515 961 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4486 4860 5006 387 Size: 1064 138 2897 374 146 1280 Fragments arranged by size: 2897 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2827 2827 Size: 5899 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2320 2892 2937 3081 3176 3191 3962 Size: 393 688 572 45 144 95 15 771 Cuts at: 3962 4559 5346 5614 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 771 688 597 572 393 268 144 95 45 15 EcoO109I rG'GnC_Cy Cuts at: 2207 2207 Size: 5899 EcoRI G'AATT_C Cuts at: 2497 3131 3208 3586 2497 Size: 634 77 378 4810 Fragments arranged by size: 4810 634 378 77 EcoRV GAT'ATC Cuts at: 2548 2548 Size: 5899 FspI TGC'GCA Cuts at: 4405 4405 Size: 5899 HaeII r_GCGC'y Cuts at: 1678 5046 1678 Size: 3368 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2248 2280 4540 4572 4614 Size: 32 135 32 676 32 2260 32 42 Cuts at: 4614 4646 1373 Size: 32 2626 Fragments arranged by size: 2626 2260 676 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2866 3243 3489 669 Size: 988 281 928 377 246 3079 Fragments arranged by size: 3079 988 928 377 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5899 HpaI GTT'AAC Cuts at: 3489 3489 Size: 5899 KpnI G_GTAC'C Cuts at: 3224 3224 Size: 5899 MfeI C'AATT_G Cuts at: 2193 2427 3498 2193 Size: 234 1071 4594 Fragments arranged by size: 4594 1071 234 MluI A'CGCG_T Cuts at: 2793 5775 2793 Size: 2982 2917 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5013 5197 1305 Size: 3708 184 2007 Fragments arranged by size: 3708 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4077 Fragments arranged by size: 4077 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3857 4216 4375 518 Size: 648 2691 359 159 2042 Fragments arranged by size: 2691 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2591 3315 3992 5140 5385 1606 Size: 985 724 677 1148 245 2120 Fragments arranged by size: 2120 1148 985 724 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4408 Fragments arranged by size: 4408 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5899 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5899 NotI GC'GGCC_GC Cuts at: 3346 3346 Size: 5899 NsiI A_TGCA'T Cuts at: 2734 2734 Size: 5899 NspI r_CATG'y Cuts at: 1132 1229 3257 4802 1132 Size: 97 2028 1545 2229 Fragments arranged by size: 2229 2028 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5899 PciI A'CATG_T Cuts at: 4798 4798 Size: 5899 PflMI CCAn_nnn'nTGG Cuts at: 1108 2198 2395 1108 Size: 1090 197 4612 Fragments arranged by size: 4612 1090 197 PmeI GTTT'AAAC Cuts at: 3203 3203 Size: 5899 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3968 4000 5481 5513 3968 Size: 32 1481 32 4354 Fragments arranged by size: 4354 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5899 PsiI TTA'TAA Cuts at: 3469 3469 Size: 5899 PspOMI G'GGCC_C Cuts at: 2206 2206 Size: 5899 PstI C_TGCA'G Cuts at: 830 2916 3251 830 Size: 2086 335 3478 Fragments arranged by size: 3478 2086 335 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5899 PvuII CAG'CTG Cuts at: 2591 2591 Size: 5899 SacI G_AGCT'C Cuts at: 2829 2829 Size: 5899 SalI G'TCGA_C Cuts at: 1936 3241 1936 Size: 1305 4594 SbfI CC_TGCA'GG Cuts at: 3251 3251 Size: 5899 ScaI AGT'ACT Cuts at: 4147 5859 4147 Size: 1712 4187 SfcI C'TryA_G Cuts at: 826 1045 2278 2348 2912 3247 4382 5063 5254 Size: 219 1233 70 564 335 1135 681 191 Cuts at: 5254 826 Size: 1471 Fragments arranged by size: 1471 1233 1135 681 564 335 219 191 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5899 SmaI CCC'GGG Cuts at: 1872 3226 1872 Size: 1354 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2265 2575 4003 4904 5166 820 Size: 197 926 322 310 1428 901 262 1553 Fragments arranged by size: 1553 1428 926 901 322 310 262 197 SnaBI TAC'GTA Cuts at: 493 3690 493 Size: 3197 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5899 SphI G_CATG'C Cuts at: 1132 3257 1132 Size: 2125 3774 SspI AAT'ATT Cuts at: 5 52 3823 5 Size: 47 3771 2081 Fragments arranged by size: 3771 2081 47 StuI AGG'CCT Cuts at: 1517 2602 1517 Size: 1085 4814 StyI C'CwwG_G Cuts at: 513 1065 2004 2502 3338 513 Size: 552 939 498 836 3074 Fragments arranged by size: 3074 939 836 552 498 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2396 3926 3943 4096 4281 4726 325 Size: 1255 816 1530 17 153 185 445 1498 Fragments arranged by size: 1530 1498 1255 816 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4145 5857 96 Size: 274 80 33 51 1443 2168 1712 138 Fragments arranged by size: 2168 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5859 5891 5859 Size: 32 5867 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5899 XbaI T'CTAG_A Cuts at: 3235 3235 Size: 5899 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5899 XmaI C'CCGG_G Cuts at: 1870 3224 1870 Size: 1354 4545 XmnI GAAnn'nnTTC Cuts at: 3057 4028 3057 Size: 971 4928 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3707 276 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI