Restriction map for
pFN21AE2316
(Linear) (Six-base) MAPSORT of: KIEE2316.seq Check: 110 from: 1 to: 1194
KIEE2316.orf
With 182 enzymes: SgfI *
January 11, 2011 13:49 ..
AccI GT'mk_AC
Cuts at: 0 276 1194
Size: 276 918
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 932 1194
Size: 887 45 262
Fragments arranged by size:
887 262 45
AflIII A'CryG_T
Cuts at: 0 788 1194
Size: 788 406
AlwNI CAG_nnn'CTG
Cuts at: 0 217 1194
Size: 217 977
ApaI G_GGCC'C
Cuts at: 0 205 1194
Size: 205 989
ApoI r'AATT_y
Cuts at: 0 178 492 1019 1032 1126 1194
Size: 178 314 527 13 94 68
Fragments arranged by size:
527 314 178 94 68 13
BamHI G'GATC_C
Cuts at: 0 942 1194
Size: 942 252
BanI G'GyrC_C
Cuts at: 0 634 1194
Size: 634 560
BanII G_rGCy'C
Cuts at: 0 205 824 1194
Size: 205 619 370
Fragments arranged by size:
619 370 205
BfrBI ATG'CAT
Cuts at: 0 727 1194
Size: 727 467
BlpI GC'TnA_GC
Cuts at: 0 651 1194
Size: 651 543
Bme1580I G_kGCm'C
Cuts at: 0 205 637 683 1194
Size: 205 432 46 511
Fragments arranged by size:
511 432 205 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 1127 1194
Size: 1127 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 1076 1171 1186 1194
Size: 315 761 95 15 8
Fragments arranged by size:
761 315 95 15 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 591 1194
Size: 245 346 603
Fragments arranged by size:
603 346 245
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1168 1194
Size: 1168 26
BseYI C'CCAG_C
Cuts at: 0 299 1194
Size: 299 895
BsiHKAI G_wGCw'C
Cuts at: 0 153 824 1194
Size: 153 671 370
Fragments arranged by size:
671 370 153
BsmI GAATG_Cn'
Cuts at: 0 727 1194
Size: 727 467
Bsp1286I G_dGCh'C
Cuts at: 0 153 205 637 683 824 1194
Size: 153 52 432 46 141 370
Fragments arranged by size:
432 370 153 141 52 46
BsrDI GCAATG_nn'
Cuts at: 0 900 1194
Size: 900 294
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1073 1194
Size: 1073 121
BstYI r'GATC_y
Cuts at: 0 89 942 1194
Size: 89 853 252
Fragments arranged by size:
853 252 89
EarI CTCTTCn'nnn_
Cuts at: 0 863 1194
Size: 863 331
EcoICRI GAG'CTC
Cuts at: 0 822 1194
Size: 822 372
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 887 932 1076 1171 1186 1194
Size: 315 572 45 144 95 15 8
Fragments arranged by size:
572 315 144 95 45 15 8
EcoO109I rG'GnC_Cy
Cuts at: 0 202 1194
Size: 202 992
EcoRI G'AATT_C
Cuts at: 0 492 1126 1194
Size: 492 634 68
Fragments arranged by size:
634 492 68
EcoRV GAT'ATC
Cuts at: 0 543 1194
Size: 543 651
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 243 275 1194
Size: 243 32 919
Fragments arranged by size:
919 243 32
HincII GTy'rAC
Cuts at: 0 861 1194
Size: 861 333
MfeI C'AATT_G
Cuts at: 0 188 422 1194
Size: 188 234 772
Fragments arranged by size:
772 234 188
MluI A'CGCG_T
Cuts at: 0 788 1194
Size: 788 406
MspA1I CmG'CkG
Cuts at: 0 586 1194
Size: 586 608
NsiI A_TGCA'T
Cuts at: 0 729 1194
Size: 729 465
PflMI CCAn_nnn'nTGG
Cuts at: 0 193 390 1194
Size: 193 197 804
Fragments arranged by size:
804 197 193
PspOMI G'GGCC_C
Cuts at: 0 201 1194
Size: 201 993
PstI C_TGCA'G
Cuts at: 0 911 1194
Size: 911 283
PvuII CAG'CTG
Cuts at: 0 586 1194
Size: 586 608
SacI G_AGCT'C
Cuts at: 0 824 1194
Size: 824 370
SfcI C'TryA_G
Cuts at: 0 273 343 907 1194
Size: 273 70 564 287
Fragments arranged by size:
564 287 273 70
SmlI C'TyrA_G
Cuts at: 0 260 570 1194
Size: 260 310 624
Fragments arranged by size:
624 310 260
StuI AGG'CCT
Cuts at: 0 597 1194
Size: 597 597
StyI C'CwwG_G
Cuts at: 0 497 1194
Size: 497 697
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 391 1194
Size: 391 803
XmnI GAAnn'nnTTC
Cuts at: 0 1052 1194
Size: 1052 142
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI ApoI BamHI BanI
BanII BfrBI BlpI Bme1580I BmrI BpmI BpuEI BseRI
BseYI BsiHKAI BsmI Bsp1286I BsrDI BstXI BstYI EarI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV Hin4I HincII MfeI
MluI MspA1I NsiI PflMI PspOMI PstI PvuII SacI
SfcI SmlI StuI StyI TaqII XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BmgBI BmtI BplI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoNI FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MmeI MscI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI