(Circular) (Six-base) MAPSORT of: pFN21AE3889.seq Check: 3750 from: 1 to: 4840 pFN21AE3889 4840 bp With 182 enzymes: SgfI * August 4, 2021 21:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2650 278 Size: 53 83 186 936 1114 2468 Fragments arranged by size: 2468 1114 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2183 1937 Size: 246 4594 Acc65I G'GTAC_C Cuts at: 2161 2161 Size: 4840 AclI AA'CG_TT Cuts at: 1563 2967 3340 1563 Size: 1404 373 3063 Fragments arranged by size: 3063 1404 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2078 2094 2903 4287 4555 1632 Size: 446 16 809 1384 268 1917 Fragments arranged by size: 1917 1384 809 446 268 16 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4643 AflIII A'CryG_T Cuts at: 1203 3739 4716 1203 Size: 2536 977 1327 Fragments arranged by size: 2536 1327 977 AgeI A'CCGG_T Cuts at: 3671 3671 Size: 4840 AhdI GACnn_n'nnGTC Cuts at: 3569 3569 Size: 4840 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4840 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4840 AlwNI CAG_nnn'CTG Cuts at: 2583 4155 2583 Size: 1572 3268 ApaLI G'TGCA_C Cuts at: 2897 4053 2897 Size: 1156 3684 ApoI r'AATT_y Cuts at: 1493 2149 2375 2527 4636 1493 Size: 656 226 152 2109 1697 Fragments arranged by size: 2109 1697 656 226 152 AseI AT'TA_AT Cuts at: 160 1032 3394 160 Size: 872 2362 1606 Fragments arranged by size: 2362 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4840 AvaI C'yCGr_G Cuts at: 1870 1943 2165 1870 Size: 73 222 4545 Fragments arranged by size: 4545 222 73 BamHI G'GATC_C Cuts at: 2170 2537 2170 Size: 367 4473 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2161 3616 618 Size: 325 217 627 374 1455 1842 Fragments arranged by size: 1842 1455 627 374 325 217 BanII G_rGCy'C Cuts at: 1391 2159 1391 Size: 768 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3869 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3031 3065 1903 Size: 34 1094 34 3678 Fragments arranged by size: 3678 1094 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2732 3942 2732 Size: 1210 3630 BclI T'GATC_A Cuts at: 1548 1548 Size: 4840 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3451 136 Size: 107 122 71 631 605 1779 1525 Fragments arranged by size: 1779 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4835 4835 Size: 4840 BlpI GC'TnA_GC Cuts at: 2257 2257 Size: 4840 Bme1580I G_kGCm'C Cuts at: 1790 2901 4057 1790 Size: 1111 1156 2573 Fragments arranged by size: 2573 1156 1111 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4840 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2571 3529 442 Size: 416 53 83 399 24 1154 958 1753 Fragments arranged by size: 1753 1154 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4840 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3500 1239 Size: 2261 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2965 3830 4128 2965 Size: 865 298 3677 Fragments arranged by size: 3677 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3503 882 Size: 617 2004 2219 Fragments arranged by size: 2219 2004 617 BsaAI yAC'GTr Cuts at: 493 1204 2631 493 Size: 711 1427 2702 Fragments arranged by size: 2702 1427 711 BsaBI GATnn'nnATC Cuts at: 4834 4834 Size: 4840 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2647 3029 3664 275 Size: 53 83 186 936 1114 382 635 1451 Fragments arranged by size: 1451 1114 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3273 3671 3945 4092 4503 3273 Size: 398 274 147 411 3610 Fragments arranged by size: 3610 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4615 Fragments arranged by size: 4615 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4840 BseYI C'CCAG_C Cuts at: 4043 4539 4043 Size: 496 4344 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4267 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2290 3051 4079 1540 Size: 275 186 289 761 1028 2301 Fragments arranged by size: 2301 1028 761 289 275 186 BsiHKAI G_wGCw'C Cuts at: 2901 2986 4057 2901 Size: 85 1071 3684 Fragments arranged by size: 3684 1071 85 BsmI GAATG_Cn' Cuts at: 2351 2444 2351 Size: 93 4747 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2159 2901 2986 4057 1391 Size: 399 369 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 369 85 BspEI T'CCGG_A Cuts at: 4503 4503 Size: 4840 BspHI T'CATG_A Cuts at: 2729 4459 2729 Size: 1730 3110 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2195 844 Size: 1351 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2727 1394 Size: 429 904 3507 Fragments arranged by size: 3507 904 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3335 3509 65 Size: 1183 2087 174 1396 Fragments arranged by size: 2087 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3484 3671 1700 Size: 110 1674 187 2869 Fragments arranged by size: 2869 1674 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4840 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4840 BssSI C'ACGA_G Cuts at: 2900 3912 2900 Size: 1012 3828 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4840 BstBI TT'CG_AA Cuts at: 3644 3644 Size: 4840 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4840 BstYI r'GATC_y Cuts at: 1972 2170 2537 2922 2939 4391 4477 4489 4835 Size: 198 367 385 17 1452 86 12 346 Cuts at: 4835 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 198 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4288 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4101 Fragments arranged by size: 4101 683 56 BtsI GCAGTG_nn' Cuts at: 2356 3150 3170 2356 Size: 794 20 4026 Fragments arranged by size: 4026 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4840 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4805 DraI TTT'AAA Cuts at: 2144 2144 Size: 4840 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3847 809 Size: 507 2531 1802 Fragments arranged by size: 2531 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2287 3176 4805 Size: 54 1425 50 275 18 457 889 1629 Cuts at: 4805 8 Size: 43 Fragments arranged by size: 1629 1425 889 457 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2287 1537 Size: 275 475 4090 Fragments arranged by size: 4090 475 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2094 2770 1353 Size: 741 676 3423 Fragments arranged by size: 3423 741 676 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3427 3801 3947 387 Size: 1064 138 1838 374 146 1280 Fragments arranged by size: 1838 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2094 2903 3500 4287 4555 1239 Size: 393 446 16 809 597 787 268 1524 Fragments arranged by size: 1524 809 787 597 446 393 268 16 EcoRI G'AATT_C Cuts at: 2149 2527 2149 Size: 378 4462 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2038 2038 Size: 4840 FspI TGC'GCA Cuts at: 3346 3346 Size: 4840 HaeII r_GCGC'y Cuts at: 1678 3987 1678 Size: 2309 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3481 3513 3555 3587 1373 Size: 32 135 32 1909 32 42 32 2626 Fragments arranged by size: 2626 1909 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2184 2430 669 Size: 988 281 246 246 3079 Fragments arranged by size: 3079 988 281 246 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4840 HpaI GTT'AAC Cuts at: 2430 2430 Size: 4840 KpnI G_GTAC'C Cuts at: 2165 2165 Size: 4840 MfeI C'AATT_G Cuts at: 2439 2439 Size: 4840 MluI A'CGCG_T Cuts at: 4716 4716 Size: 4840 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2007 3954 4138 1305 Size: 702 1947 184 2007 Fragments arranged by size: 2007 1947 702 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3018 Fragments arranged by size: 3018 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2798 3157 3316 518 Size: 648 1632 359 159 2042 Fragments arranged by size: 2042 1632 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2256 2933 4081 4326 1606 Size: 650 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 650 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4288 NdeI CA'TA_TG Cuts at: 387 387 Size: 4840 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4840 NotI GC'GGCC_GC Cuts at: 2287 2287 Size: 4840 NspI r_CATG'y Cuts at: 1132 1229 2198 3743 1132 Size: 97 969 1545 2229 Fragments arranged by size: 2229 1545 969 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4840 PciI A'CATG_T Cuts at: 3739 3739 Size: 4840 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4840 PmeI GTTT'AAAC Cuts at: 2144 2144 Size: 4840 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2909 2941 4422 4454 2909 Size: 32 1481 32 3295 Fragments arranged by size: 3295 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4840 PsiI TTA'TAA Cuts at: 2410 2410 Size: 4840 PstI C_TGCA'G Cuts at: 830 2192 830 Size: 1362 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4840 SalI G'TCGA_C Cuts at: 1936 2182 1936 Size: 246 4594 SbfI CC_TGCA'GG Cuts at: 2192 2192 Size: 4840 ScaI AGT'ACT Cuts at: 3088 4800 3088 Size: 1712 3128 SfcI C'TryA_G Cuts at: 826 1045 2188 3323 4004 4195 826 Size: 219 1143 1135 681 191 1471 Fragments arranged by size: 1471 1143 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4840 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4840 SmaI CCC'GGG Cuts at: 1872 2167 1872 Size: 295 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2944 3845 4107 820 Size: 197 926 1001 901 262 1553 Fragments arranged by size: 1553 1001 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2631 493 Size: 2138 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4840 SphI G_CATG'C Cuts at: 1132 2198 1132 Size: 1066 3774 SspI AAT'ATT Cuts at: 5 52 2764 5 Size: 47 2712 2081 Fragments arranged by size: 2712 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4840 StyI C'CwwG_G Cuts at: 513 1065 2279 513 Size: 552 1214 3074 Fragments arranged by size: 3074 1214 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2867 2884 3037 3222 3667 325 Size: 1255 1287 17 153 185 445 1498 Fragments arranged by size: 1498 1287 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3086 4798 96 Size: 274 80 33 51 1443 1109 1712 138 Fragments arranged by size: 1712 1443 1109 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4800 4832 4800 Size: 32 4808 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4840 XbaI T'CTAG_A Cuts at: 2176 2176 Size: 4840 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4840 XmaI C'CCGG_G Cuts at: 1870 2165 1870 Size: 295 4545 XmnI GAAnn'nnTTC Cuts at: 2969 2969 Size: 4840 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2648 276 Size: 53 83 186 936 1114 2468 Fragments arranged by size: 2468 1114 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI