################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BseRI BsiHKAI BsiWI BsmBI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KspI MamI MluI MluNI MroI MscI MseI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 1028 GGTACC G^GTACC AflIII 1022 ACATGT A^C[GA][CT]GT AlwI 817 GGATC GGATC(4/5) Asp700I 33 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1028 GGTACC G^GTACC AvaI 1058 CTCGGG C^[CT]CG[GA]G AvaII 678 GGACC G^G[AT]CC BanI 1028 GGTACC G^G[CT][GA]CC BcgI 427 CGAAGAAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 705 CCGGG CC^[GC]GG BglI 731 GCCTGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 823 GAGACC GGTCTC(1/5) BsaJI 1057 CCTCGG C^C[ACGT][ACGT]GG BsaWI 126 TCCGGT [AT]^CCGG[AT] BsgI 663 GTGCAG GTGCAG(16/14) BsiEI 738 CGGCCG CG[GA][CT]^CG BsmFI 115 GTCCC GGGAC(10/14) BsoBI 1058 CTCGGG C^[CT]CG[GA]G BspLU11I 1022 ACATGT A^CATGT BsrDI 980 CATTGC GCAATG(2/0) BstYI 817 GGATCT [GA]^GATC[CT] CfoI 268 GCGC GCG^C EaeI 738 CGGCCG [CT]^GGCC[GA] EagI 738 CGGCCG C^GGCCG EarI 634 GAAGAG CTCTTC(1/4) EclXI 738 CGGCCG C^GGCCG Eco52I 738 CGGCCG C^GGCCG EcoNI 1057 CCTCGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 90 GAATTC G^AATTC HhaI 268 GCGC GCG^C HinP1I 268 GCGC G^CGC HincII 766 GTTGAC GT[CT]^[GA]AC HindII 766 GTTGAC GT[CT]^[GA]AC KpnI 1028 GGTACC GGTAC^C Ksp632I 634 GAAGAG CTCTTC(1/4) MaeII 962 ACGT A^CGT MfeI 105 CAATTG C^AATTG MflI 817 GGATCT [GA]^GATC[CT] MslI 1011 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 517 CAGCTG C[AC]G^C[GT]G MunI 105 CAATTG C^AATTG NciI 705 CCGGG CC^[GC]GG PvuII 517 CAGCTG CAG^CTG SapI 634 GAAGAGC GCTCTTC(1/4) ScaI 875 AGTACT AGT^ACT SfaNI 312 GATGC GCATC(5/9) SphI 157 GCATGC GCATG^C XcmI 578 CCAAAAATAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 817 GGATCT [GA]^GATC[CT] XmnI 33 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BfaI 179 CTAG C^TAG 646 CTAG BpmI 367 CTCCAG CTGGAG(16/14) 589 CTGGAG BspMI 920 ACCTGC ACCTGC(4/8) 1045 ACCTGC BstAPI 846 GCAATTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 863 GCAACTTGTGC BstXI 516 CCAGCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1010 CCATCCTGGTGG HapII 127 CCGG C^CGG 705 CCGG HpaII 127 CCGG C^CGG 705 CCGG HphI 615 GGTGA GGTGA(8/7) 1004 TCACC MaeI 179 CTAG C^TAG 646 CTAG MspI 127 CCGG C^CGG 705 CCGG NspI 157 GCATGC [GA]CATG^[CT] 1022 ACATGT PleI 967 GACTC GAGTC(4/5) 592 GAGTC PstI 853 CTGCAG CTGCA^G 922 CTGCAG SfcI 853 CTGCAG C^T[GA][CT]AG 922 CTGCAG TaqI 426 TCGA T^CGA 632 TCGA Tsp45I 46 GTCAC ^GT[GC]AC 404 GTGAC TthHB8I 426 TCGA T^CGA 632 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BbvI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TfiI TseI Tsp509I TspRI