(Circular) (Six-base) MAPSORT of: pFN21AE5542.seq Check: 5813 from: 1 to: 5800 pFN21AE5542 5800 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3059 3059 Size: 5800 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3610 278 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3143 1937 Size: 1206 4594 Acc65I G'GTAC_C Cuts at: 3033 3121 3033 Size: 88 5712 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2477 2651 3863 5247 5515 1632 Size: 845 174 1212 1384 268 1917 Fragments arranged by size: 1917 1384 1212 845 268 174 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5603 AflIII A'CryG_T Cuts at: 1203 3027 4699 5676 1203 Size: 1824 1672 977 1327 Fragments arranged by size: 1824 1672 1327 977 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 4529 4529 Size: 5800 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5800 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5800 AlwNI CAG_nnn'CTG Cuts at: 3543 5115 3543 Size: 1572 4228 ApaLI G'TGCA_C Cuts at: 3857 5013 3857 Size: 1156 4644 ApoI r'AATT_y Cuts at: 1493 2095 2473 2907 3109 3335 3487 5596 1493 Size: 602 378 434 202 226 152 2109 1697 Fragments arranged by size: 2109 1697 602 434 378 226 202 152 AseI AT'TA_AT Cuts at: 160 1032 4354 160 Size: 872 3322 1606 Fragments arranged by size: 3322 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 3063 3125 1870 Size: 73 1120 62 4545 Fragments arranged by size: 4545 1120 73 62 BamHI G'GATC_C Cuts at: 3130 3497 3130 Size: 367 5433 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3033 3121 4576 618 Size: 325 217 627 1246 88 1455 1842 Fragments arranged by size: 1842 1455 1246 627 325 217 88 BanII G_rGCy'C Cuts at: 1391 3119 1391 Size: 1728 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4829 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2421 2455 3991 4025 1903 Size: 34 484 34 1536 34 3678 Fragments arranged by size: 3678 1536 484 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3692 4902 3692 Size: 1210 4590 BclI T'GATC_A Cuts at: 1548 1548 Size: 5800 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2742 4411 136 Size: 107 122 71 631 605 1070 1669 1525 Fragments arranged by size: 1669 1525 1070 631 605 122 107 71 BglII A'GATC_T Cuts at: 5795 5795 Size: 5800 BlpI GC'TnA_GC Cuts at: 3217 3217 Size: 5800 Bme1580I G_kGCm'C Cuts at: 1790 3861 5017 1790 Size: 2071 1156 2573 Fragments arranged by size: 2573 2071 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5800 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3531 4489 442 Size: 416 53 83 399 24 2114 958 1753 Fragments arranged by size: 2114 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2357 2615 4460 1239 Size: 1118 258 1845 2579 Fragments arranged by size: 2579 1845 1118 258 Bpu10I CC'TnA_GC Cuts at: 2481 2481 Size: 5800 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3925 4790 5088 3925 Size: 865 298 4637 Fragments arranged by size: 4637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2822 4463 882 Size: 617 1323 1641 2219 Fragments arranged by size: 2219 1641 1323 617 BsaAI yAC'GTr Cuts at: 493 1204 3591 493 Size: 711 2387 2702 Fragments arranged by size: 2702 2387 711 BsaBI GATnn'nnATC Cuts at: 5794 5794 Size: 5800 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3607 3989 4624 275 Size: 53 83 186 936 2074 382 635 1451 Fragments arranged by size: 2074 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2131 4233 4631 4905 5052 5463 2131 Size: 2102 398 274 147 411 2468 Fragments arranged by size: 2468 2102 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2336 2366 2588 2618 2957 Size: 30 165 30 933 30 222 30 339 Cuts at: 2957 2987 2996 3026 1178 Size: 30 9 30 3952 Fragments arranged by size: 3952 933 339 222 165 30 30 30 30 30 30 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5800 BseYI C'CCAG_C Cuts at: 2355 5003 5499 2355 Size: 2648 496 2656 Fragments arranged by size: 2656 2648 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2689 1163 Size: 573 953 4274 Fragments arranged by size: 4274 953 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2746 3250 4011 5039 1540 Size: 275 186 745 504 761 1028 2301 Fragments arranged by size: 2301 1028 761 745 504 275 186 BsiHKAI G_wGCw'C Cuts at: 3861 3946 5017 3861 Size: 85 1071 4644 Fragments arranged by size: 4644 1071 85 BsmI GAATG_Cn' Cuts at: 3311 3404 3311 Size: 93 5707 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3119 3861 3946 5017 1391 Size: 399 1329 742 85 1071 2174 Fragments arranged by size: 2174 1329 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5463 5463 Size: 5800 BspHI T'CATG_A Cuts at: 3689 5419 3689 Size: 1730 4070 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2934 3059 3155 844 Size: 2090 125 96 3489 Fragments arranged by size: 3489 2090 125 96 BsrBI CCG'CTC Cuts at: 1394 1823 3687 1394 Size: 429 1864 3507 Fragments arranged by size: 3507 1864 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2984 4295 4469 65 Size: 1183 1736 1311 174 1396 Fragments arranged by size: 1736 1396 1311 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4444 4631 1700 Size: 110 2634 187 2869 Fragments arranged by size: 2869 2634 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5800 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5800 BssSI C'ACGA_G Cuts at: 3860 4872 3860 Size: 1012 4788 BstAPI GCAn_nnn'nTGC Cuts at: 794 2857 2874 794 Size: 2063 17 3720 Fragments arranged by size: 3720 2063 17 BstBI TT'CG_AA Cuts at: 4604 4604 Size: 5800 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5800 BstXI CCAn_nnnn'nTGG Cuts at: 2528 3022 2528 Size: 494 5306 BstYI r'GATC_y Cuts at: 1972 2822 3130 3497 3882 3899 5351 5437 5449 Size: 850 308 367 385 17 1452 86 12 Cuts at: 5449 5795 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 850 385 367 346 308 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5248 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5061 Fragments arranged by size: 5061 683 56 BtsI GCAGTG_nn' Cuts at: 2122 3316 4110 4130 2122 Size: 1194 794 20 3792 Fragments arranged by size: 3792 1194 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5765 DraI TTT'AAA Cuts at: 3104 3104 Size: 5800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4807 809 Size: 507 3491 1802 Fragments arranged by size: 3491 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2743 3247 4136 Size: 54 1425 50 275 18 913 504 889 Cuts at: 4136 5765 8 Size: 1629 43 Fragments arranged by size: 1629 1425 913 889 504 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2743 3247 1537 Size: 275 931 504 4090 Fragments arranged by size: 4090 931 504 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2634 3730 1353 Size: 1281 1096 3423 Fragments arranged by size: 3423 1281 1096 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2026 4387 4761 4907 387 Size: 1064 138 437 2361 374 146 1280 Fragments arranged by size: 2361 1280 1064 437 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2357 2477 2615 2651 3863 4460 5247 Size: 393 725 120 138 36 1212 597 787 Cuts at: 5247 5515 1239 Size: 268 1524 Fragments arranged by size: 1524 1212 787 725 597 393 268 138 120 36 EcoNI CCTnn'n_nnAGG Cuts at: 3066 3066 Size: 5800 EcoRI G'AATT_C Cuts at: 2095 3109 3487 2095 Size: 1014 378 4408 Fragments arranged by size: 4408 1014 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2830 2830 Size: 5800 FspI TGC'GCA Cuts at: 4306 4306 Size: 5800 HaeII r_GCGC'y Cuts at: 1678 4947 1678 Size: 3269 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2474 2506 2560 2592 2996 Size: 32 135 32 902 32 54 32 404 Cuts at: 2996 3028 4441 4473 4515 4547 1373 Size: 32 1413 32 42 32 2626 Fragments arranged by size: 2626 1413 902 404 135 54 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2773 3144 3390 669 Size: 988 281 835 371 246 3079 Fragments arranged by size: 3079 988 835 371 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5800 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KpnI G_GTAC'C Cuts at: 3037 3125 3037 Size: 88 5712 MfeI C'AATT_G Cuts at: 2110 3399 2110 Size: 1289 4511 MluI A'CGCG_T Cuts at: 5676 5676 Size: 5800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4914 5098 1305 Size: 3609 184 2007 Fragments arranged by size: 3609 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3978 Fragments arranged by size: 3978 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3020 3758 4117 4276 518 Size: 648 1854 738 359 159 2042 Fragments arranged by size: 2042 1854 738 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2524 3216 3893 5041 5286 1606 Size: 918 692 677 1148 245 2120 Fragments arranged by size: 2120 1148 918 692 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5248 NdeI CA'TA_TG Cuts at: 387 387 Size: 5800 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5800 NotI GC'GGCC_GC Cuts at: 3247 3247 Size: 5800 NspI r_CATG'y Cuts at: 1132 1229 2166 3031 3158 4703 1132 Size: 97 937 865 127 1545 2229 Fragments arranged by size: 2229 1545 937 865 127 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5800 PciI A'CATG_T Cuts at: 3027 4699 3027 Size: 1672 4128 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5800 PfoI T'CCnGG_A Cuts at: 2709 2709 Size: 5800 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3869 3901 5382 5414 3869 Size: 32 1481 32 4255 Fragments arranged by size: 4255 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5800 PsiI TTA'TAA Cuts at: 2217 3370 2217 Size: 1153 4647 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2327 2359 2327 Size: 32 5768 PstI C_TGCA'G Cuts at: 830 2862 2931 3152 830 Size: 2032 69 221 3478 Fragments arranged by size: 3478 2032 221 69 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5800 PvuII CAG'CTG Cuts at: 2524 2524 Size: 5800 SalI G'TCGA_C Cuts at: 1936 3142 1936 Size: 1206 4594 SapI GCTCTTCn'nnn_ Cuts at: 2634 2634 Size: 5800 SbfI CC_TGCA'GG Cuts at: 3152 3152 Size: 5800 ScaI AGT'ACT Cuts at: 2882 4048 5760 2882 Size: 1166 1712 2922 Fragments arranged by size: 2922 1712 1166 SfcI C'TryA_G Cuts at: 826 1045 2858 2927 3148 4283 4964 5155 826 Size: 219 1813 69 221 1135 681 191 1471 Fragments arranged by size: 1813 1471 1135 681 221 219 191 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5800 SmaI CCC'GGG Cuts at: 1872 3127 1872 Size: 1255 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3904 4805 5067 820 Size: 197 926 1961 901 262 1553 Fragments arranged by size: 1961 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3591 493 Size: 3098 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5800 SphI G_CATG'C Cuts at: 1132 2166 3158 1132 Size: 1034 992 3774 Fragments arranged by size: 3774 1034 992 SspI AAT'ATT Cuts at: 5 52 3724 5 Size: 47 3672 2081 Fragments arranged by size: 3672 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5800 StyI C'CwwG_G Cuts at: 513 1065 3239 513 Size: 552 2174 3074 Fragments arranged by size: 3074 2174 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3827 3844 3997 4182 4627 325 Size: 1255 2247 17 153 185 445 1498 Fragments arranged by size: 2247 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2880 4046 5758 Size: 274 80 33 51 1443 903 1166 1712 Cuts at: 5758 96 Size: 138 Fragments arranged by size: 1712 1443 1166 903 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2588 2620 2941 2973 5760 5792 2588 Size: 32 321 32 2787 32 2596 Fragments arranged by size: 2787 2596 321 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5800 XbaI T'CTAG_A Cuts at: 3136 3136 Size: 5800 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2590 2590 Size: 5800 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5800 XmaI C'CCGG_G Cuts at: 1870 3125 1870 Size: 1255 4545 XmnI GAAnn'nnTTC Cuts at: 2042 3929 2042 Size: 1887 3913 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3608 276 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI