(Linear) (Six-base) MAPSORT of: KIEE5542.seq Check: 6997 from: 1 to: 1095 KIEE5542.orf With 182 enzymes: SgfI * January 29, 2014 12:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1054 1095 Size: 1054 41 Acc65I G'GTAC_C Cuts at: 0 1028 1095 Size: 1028 67 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 646 1095 Size: 472 174 449 Fragments arranged by size: 472 449 174 AflIII A'CryG_T Cuts at: 0 1022 1095 Size: 1022 73 ApoI r'AATT_y Cuts at: 0 90 468 902 1095 Size: 90 378 434 193 Fragments arranged by size: 434 378 193 90 AvaI C'yCGr_G Cuts at: 0 1058 1095 Size: 1058 37 BanI G'GyrC_C Cuts at: 0 1028 1095 Size: 1028 67 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 416 450 1095 Size: 416 34 645 Fragments arranged by size: 645 416 34 BglI GCCn_nnn'nGGC Cuts at: 0 737 1095 Size: 737 358 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 352 610 1095 Size: 352 258 485 Fragments arranged by size: 485 352 258 Bpu10I CC'TnA_GC Cuts at: 0 476 1095 Size: 476 619 BsaI GGTCTCn'nnnn_ Cuts at: 0 817 1095 Size: 817 278 BsaWI w'CCGG_w Cuts at: 0 126 1095 Size: 126 969 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 331 361 583 613 952 982 991 1021 Size: 331 30 222 30 339 30 9 30 Cuts at: 1021 1095 Size: 74 Fragments arranged by size: 339 331 222 74 30 30 30 30 9 BseYI C'CCAG_C Cuts at: 0 350 1095 Size: 350 745 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 684 1095 Size: 684 411 BsiEI CG_ry'CG Cuts at: 0 741 1095 Size: 741 354 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 929 1054 1095 Size: 929 125 41 Fragments arranged by size: 929 125 41 BsrDI GCAATG_nn' Cuts at: 0 979 1095 Size: 979 116 BstAPI GCAn_nnn'nTGC Cuts at: 0 852 869 1095 Size: 852 17 226 Fragments arranged by size: 852 226 17 BstXI CCAn_nnnn'nTGG Cuts at: 0 523 1017 1095 Size: 523 494 78 Fragments arranged by size: 523 494 78 BstYI r'GATC_y Cuts at: 0 817 1095 Size: 817 278 BtsI GCAGTG_nn' Cuts at: 0 117 1095 Size: 117 978 EaeI y'GGCC_r Cuts at: 0 738 1095 Size: 738 357 EagI C'GGCC_G Cuts at: 0 738 1095 Size: 738 357 EarI CTCTTCn'nnn_ Cuts at: 0 629 1095 Size: 629 466 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 21 1095 Size: 21 1074 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 352 472 610 646 1095 Size: 352 120 138 36 449 Fragments arranged by size: 449 352 138 120 36 EcoNI CCTnn'n_nnAGG Cuts at: 0 1061 1095 Size: 1061 34 EcoRI G'AATT_C Cuts at: 0 90 1095 Size: 90 1005 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 825 1095 Size: 825 270 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 469 501 555 587 991 1023 1095 Size: 469 32 54 32 404 32 72 Fragments arranged by size: 469 404 72 54 32 32 32 HincII GTy'rAC Cuts at: 0 768 1095 Size: 768 327 KpnI G_GTAC'C Cuts at: 0 1032 1095 Size: 1032 63 MfeI C'AATT_G Cuts at: 0 105 1095 Size: 105 990 MslI CAynn'nnrTG Cuts at: 0 1015 1095 Size: 1015 80 MspA1I CmG'CkG Cuts at: 0 519 1095 Size: 519 576 NspI r_CATG'y Cuts at: 0 161 1026 1095 Size: 161 865 69 Fragments arranged by size: 865 161 69 PciI A'CATG_T Cuts at: 0 1022 1095 Size: 1022 73 PfoI T'CCnGG_A Cuts at: 0 704 1095 Size: 704 391 PsiI TTA'TAA Cuts at: 0 212 1095 Size: 212 883 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 322 354 1095 Size: 322 32 741 Fragments arranged by size: 741 322 32 PstI C_TGCA'G Cuts at: 0 857 926 1095 Size: 857 69 169 Fragments arranged by size: 857 169 69 PvuII CAG'CTG Cuts at: 0 519 1095 Size: 519 576 SapI GCTCTTCn'nnn_ Cuts at: 0 629 1095 Size: 629 466 ScaI AGT'ACT Cuts at: 0 877 1095 Size: 877 218 SfcI C'TryA_G Cuts at: 0 853 922 1095 Size: 853 69 173 Fragments arranged by size: 853 173 69 SphI G_CATG'C Cuts at: 0 161 1095 Size: 161 934 TatI w'GTAC_w Cuts at: 0 875 1095 Size: 875 220 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 583 615 936 968 1095 Size: 583 32 321 32 127 Fragments arranged by size: 583 321 127 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 585 1095 Size: 585 510 XmnI GAAnn'nnTTC Cuts at: 0 37 1095 Size: 37 1058 Enzymes that do cut: AarI Acc65I AcuI AflIII ApoI AvaI BanI BcgI BglI BpmI Bpu10I BsaI BsaWI BsaXI BseYI BsgI BsiEI BspMI BsrDI BstAPI BstXI BstYI BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI Hin4I HincII KpnI MfeI MslI MspA1I NspI PciI PfoI PsiI PsrI PstI PvuII SapI ScaI SfcI SphI TatI TstI XcmI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsaHI BseRI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoO109I EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI