################################## # NO CUT ENZYMES ( 195 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AlwI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MspI MunI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tru9I Tsp45I Tsp509I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccII 24 CGCG CG^CG AluI 101 AGCT AG^CT Alw44I 106 GTGCAC G^TGCAC AlwNI 97 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 106 GTGCAC G^TGCAC AspHI 106 GTGCAC G[AT]GC[AT]^C AvaII 45 GGACC G^G[AT]CC BbvCI 175 GCTGAGG CCTCAGC(-5/-2) BmyI 106 GTGCAC G[AGT]GC[ACT]^C BsgI 38 GTGCAG GTGCAG(16/14) BsiHKAI 106 GTGCAC G[AT]GC[AT]^C BsiYI 96 CCAGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 96 CCAGCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 76 GAGAC GTCTC(1/5) Bsp1286I 106 GTGCAC G[AGT]GC[ACT]^C BssKI 151 CCTGG ^CC[ACGT]GG BstAPI 99 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 151 CCTGG CC^[AT]GG BstUI 24 CGCG CG^CG DsaI 23 CCGCGG C^C[GA][CT]GG EarI 15 CTCTTC CTCTTC(1/4) EcoRII 151 CCTGG ^CC[AT]GG Ksp632I 15 CTCTTC CTCTTC(1/4) KspI 23 CCGCGG CCGC^GG MboII 16 TCTTC GAAGA(8/7) MvaI 151 CCTGG CC^[AT]GG MvnI 24 CGCG CG^CG NlaIII 141 CATG CATG^ NlaIV 45 GGACCC GG[ACGT]^[ACGT]CC PflMI 96 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 78 GACTC GAGTC(4/5) PvuII 100 CAGCTG CAG^CTG SacII 23 CCGCGG CCGC^GG ScrFI 151 CCTGG CC^[ACGT]GG StuI 179 AGGCCT AGG^CCT TspRI 41 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 96 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### Aor51HI 89 AGCGCT AGC^GCT 203 AGCGCT BsaJI 23 CCGCGG C^C[ACGT][ACGT]GG 151 CCTGGG BseRI 9 CTCCTC GAGGAG(10/8) 12 CTCCTC CfoI 90 GCGC GCG^C 204 GCGC Cfr13I 45 GGACC G^G[ACGT]CC 154 GGGCC DdeI 176 CTGAG C^T[ACGT]AG 273 CTCAG Eco47III 89 AGCGCT AGC^GCT 203 AGCGCT FauI 48 CCCGC CCCGC(4/6) 28 GCGGG HaeII 89 AGCGCT [GA]GCGC^[CT] 203 AGCGCT HaeIII 155 GGCC GG^CC 180 GGCC HhaI 90 GCGC GCG^C 204 GCGC HinP1I 90 GCGC G^CGC 204 GCGC MspA1I 23 CCGCGG C[AC]G^C[GT]G 100 CAGCTG Sau96I 45 GGACC G^G[ACGT]CC 154 GGGCC TaqI 68 TCGA T^CGA 237 TCGA TfiI 4 GATTC G^A[AT]TC 234 GAATC TthHB8I 68 TCGA T^CGA 237 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AciI BbvI BsrI Fnu4HI HinfI ItaI MnlI MwoI TseI