Restriction map for
pFN21AE9623
##################################
# NO CUT ENZYMES ( 143 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoT22I FauI FseI FspI HaeII
HgaI HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1956 GACGTC GACGT^C
AciI 1847 CCGC CCGC(-3/-1)
AcyI 1956 GACGTC G[GA]^CG[CT]C
AlwNI 1483 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1024 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 807 GAAGAC GAAGAC(2/6)
BcgI 1821 GCACAGATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 174 TGATCA T^GATCA
BcnI 840 CCGGG CC^[GC]GG
BpuAI 807 GAAGAC GAAGAC(2/6)
BsaHI 1956 GACGTC G[GA]^CG[CT]C
BseRI 1774 CTCCTC GAGGAG(10/8)
BstAPI 2246 GCATAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1244 CCAACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 819 GCTTGC GC[ACGT]^[ACGT]GC
DraI 2037 TTTAAA TTT^AAA
DraIII 347 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1441 CTCTTC CTCTTC(1/4)
EcoRV 1826 GATATC GAT^ATC
FbaI 174 TGATCA T^GATCA
HapII 840 CCGG C^CGG
Hin1I 1956 GACGTC G[GA]^CG[CT]C
HpaII 840 CCGG C^CGG
Ksp632I 1441 CTCTTC CTCTTC(1/4)
MspA1I 1804 CAGCTG C[AC]G^C[GT]G
MspI 840 CCGG C^CGG
NciI 840 CCGGG CC^[GC]GG
NspI 165 ACATGC [GA]CATG^[CT]
PvuII 1804 CAGCTG CAG^CTG
SacI 2124 GAGCTC GAGCT^C
SapI 1440 GCTCTTC GCTCTTC(1/4)
SfcI 2089 CTACAG C^T[GA][CT]AG
SmlI 2064 CTTGAG C^T[CT][GA]AG
SspI 340 AATATT AAT^ATT
TaqI 115 TCGA T^CGA
TthHB8I 115 TCGA T^CGA
XbaI 1563 TCTAGA T^CTAGA
XmnI 1024 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspHI 934 GTGCTC G[AT]GC[AT]^C
2124 GAGCTC
BamHI 102 GGATCC G^GATCC
1582 GGATCC
BanII 1089 GGGCTC G[GA]GC[CT]^C
2124 GAGCTC
BbvCI 1776 CCTCAGC CCTCAGC(-5/-2)
1155 GCTGAGG
BciVI 1254 GGATAC GTATCC(6/5)
1785 GGATAC
BsgI 1672 CTGCAC GTGCAG(16/14)
1769 CTGCAC
BsiHKAI 934 GTGCTC G[AT]GC[AT]^C
2124 GAGCTC
BsiYI 1259 CCATCATCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1776 CCTCAGCCTGG
BslI 1259 CCATCATCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1776 CCTCAGCCTGG
BsmAI 1567 GAGAC GTCTC(1/5)
442 GTCTC
BsmFI 843 GGGAC GGGAC(10/14)
227 GTCCC
BsmI 689 GAATGC GAATGC(1/-1)
1309 GCATTC
CfoI 1435 GCGC GCG^C
1644 GCGC
DraII 225 AGGTCCC [GA]G^G[ACGT]CC[CT]
843 GGGACCT
EcoO109I 225 AGGTCCC [GA]G^G[ACGT]CC[CT]
843 GGGACCT
EcoRI 789 GAATTC G^AATTC
912 GAATTC
EcoT14I 260 CCTTGG C^C[AT][AT]GG
479 CCAAGG
HaeIII 678 GGCC GG^CC
1342 GGCC
HhaI 1435 GCGC GCG^C
1644 GCGC
HinP1I 1435 GCGC G^CGC
1644 GCGC
HindIII 236 AAGCTT A^AGCTT
1035 AAGCTT
MaeII 26 ACGT A^CGT
1957 ACGT
MslI 166 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1533 CATTCACATG
PpuMI 225 AGGTCCC [GA]G^G[AT]CC[CT]
843 GGGACCT
StyI 260 CCTTGG C^C[AT][AT]GG
479 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BfaI BmyI BpmI
BsaJI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HinfI
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII