(Circular) (Six-base) MAPSORT of: pFN21AE9623.seq Check: 3669 from: 1 to: 6973 pFN21AE9623 6973 bp With 182 enzymes: SgfI * March 28, 2012 12:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3965 4783 278 Size: 53 83 186 936 2429 818 2468 Fragments arranged by size: 2468 2429 936 818 186 83 53 AccI GT'mk_AC Cuts at: 1937 4316 1937 Size: 2379 4594 Acc65I G'GTAC_C Cuts at: 4294 4294 Size: 6973 AclI AA'CG_TT Cuts at: 1563 5100 5473 1563 Size: 3537 373 3063 Fragments arranged by size: 3537 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2208 2304 2663 2888 3356 3470 5036 6420 Size: 576 96 359 225 468 114 1566 1384 Cuts at: 6420 6688 1632 Size: 268 1917 Fragments arranged by size: 1917 1566 1384 576 468 359 268 225 114 96 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6776 AflIII A'CryG_T Cuts at: 1203 5872 6849 1203 Size: 4669 977 1327 Fragments arranged by size: 4669 1327 977 AgeI A'CCGG_T Cuts at: 5804 5804 Size: 6973 AhdI GACnn_n'nnGTC Cuts at: 5702 5702 Size: 6973 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6973 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2604 1184 Size: 1420 5553 AlwNI CAG_nnn'CTG Cuts at: 3493 4716 6288 3493 Size: 1223 1572 4178 Fragments arranged by size: 4178 1572 1223 ApaLI G'TGCA_C Cuts at: 5030 6186 5030 Size: 1156 5817 ApoI r'AATT_y Cuts at: 1493 2363 2794 2917 3242 3841 3920 4282 4508 Size: 870 431 123 325 599 79 362 226 Cuts at: 4508 4660 6769 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 870 599 431 362 325 226 152 123 79 AseI AT'TA_AT Cuts at: 160 1032 5527 160 Size: 872 4495 1606 Fragments arranged by size: 4495 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6973 AvaI C'yCGr_G Cuts at: 1870 1943 4298 1870 Size: 73 2355 4545 Fragments arranged by size: 4545 2355 73 BamHI G'GATC_C Cuts at: 2107 3587 4303 4670 2107 Size: 1480 716 367 4410 Fragments arranged by size: 4410 1480 716 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4294 5749 618 Size: 325 217 627 2507 1455 1842 Fragments arranged by size: 2507 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3098 4133 4292 1391 Size: 1707 1035 159 4072 Fragments arranged by size: 4072 1707 1035 159 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2819 928 Size: 971 920 5082 Fragments arranged by size: 5082 971 920 BbvCI CC'TCA_GC Cuts at: 3161 3782 3161 Size: 621 6352 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3815 3849 5164 5198 1903 Size: 34 1878 34 1315 34 3678 Fragments arranged by size: 3678 1878 1315 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3253 3784 4865 6075 3253 Size: 531 1081 1210 4151 Fragments arranged by size: 4151 1210 1081 531 BclI T'GATC_A Cuts at: 1548 2179 1548 Size: 631 6342 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5584 136 Size: 107 122 71 631 605 3912 1525 Fragments arranged by size: 3912 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6968 6968 Size: 6973 BlpI GC'TnA_GC Cuts at: 4390 4390 Size: 6973 Bme1580I G_kGCm'C Cuts at: 1790 2600 5034 6190 1790 Size: 810 2434 1156 2573 Fragments arranged by size: 2573 2434 1156 810 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6973 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4704 5662 442 Size: 416 53 83 399 24 3287 958 1753 Fragments arranged by size: 3287 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6973 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3351 3351 Size: 6973 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2693 2841 4038 5633 1239 Size: 1454 148 1197 1595 2579 Fragments arranged by size: 2579 1595 1454 1197 148 Bpu10I CC'TnA_GC Cuts at: 3161 3782 3161 Size: 621 6352 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4090 5098 5963 6261 4090 Size: 1008 865 298 4802 Fragments arranged by size: 4802 1008 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5636 882 Size: 617 4137 2219 Fragments arranged by size: 4137 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4764 493 Size: 711 3560 2702 Fragments arranged by size: 3560 2702 711 BsaBI GATnn'nnATC Cuts at: 6967 6967 Size: 6973 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3962 4780 5162 5797 Size: 53 83 186 936 2429 818 382 635 Cuts at: 5797 275 Size: 1451 Fragments arranged by size: 2429 1451 936 818 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5406 5804 6078 6225 6636 5406 Size: 398 274 147 411 5743 Fragments arranged by size: 5743 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6748 Fragments arranged by size: 6748 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3770 1186 Size: 2584 4389 BseYI C'CCAG_C Cuts at: 2407 6176 6672 2407 Size: 3769 496 2708 Fragments arranged by size: 3769 2708 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3662 3759 1163 Size: 573 1926 97 4377 Fragments arranged by size: 4377 1926 573 97 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4423 5184 6212 1540 Size: 275 186 2422 761 1028 2301 Fragments arranged by size: 2422 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2943 4133 5034 5119 6190 2943 Size: 1190 901 85 1071 3726 Fragments arranged by size: 3726 1190 1071 901 85 BsmI GAATG_Cn' Cuts at: 2700 3314 4484 4577 2700 Size: 614 1170 93 5096 Fragments arranged by size: 5096 1170 614 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2600 2943 3098 4133 4292 5034 5119 Size: 399 810 343 155 1035 159 742 85 Cuts at: 5119 6190 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1035 810 742 399 343 159 155 85 BspEI T'CCGG_A Cuts at: 6636 6636 Size: 6973 BspHI T'CATG_A Cuts at: 4862 6592 4862 Size: 1730 5243 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4328 844 Size: 3484 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4860 1394 Size: 429 3037 3507 Fragments arranged by size: 3507 3037 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2520 2576 3067 3651 5468 5642 65 Size: 1183 1272 56 491 584 1817 174 1396 Fragments arranged by size: 1817 1396 1272 1183 584 491 174 56 BsrFI r'CCGG_y Cuts at: 1700 1810 5617 5804 1700 Size: 110 3807 187 2869 Fragments arranged by size: 3807 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6973 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6973 BssSI C'ACGA_G Cuts at: 5033 6045 5033 Size: 1012 5961 BstAPI GCAn_nnn'nTGC Cuts at: 794 4257 794 Size: 3463 3510 BstBI TT'CG_AA Cuts at: 5777 5777 Size: 6973 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6973 BstXI CCAn_nnnn'nTGG Cuts at: 3256 3256 Size: 6973 BstYI r'GATC_y Cuts at: 1972 2107 3587 3762 4303 4670 5055 5072 6524 Size: 135 1480 175 541 367 385 17 1452 Cuts at: 6524 6610 6622 6968 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1480 1452 541 385 367 346 175 135 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6421 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6234 Fragments arranged by size: 6234 683 56 BtsI GCAGTG_nn' Cuts at: 2217 4489 5283 5303 2217 Size: 2272 794 20 3887 Fragments arranged by size: 3887 2272 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6973 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6938 DraI TTT'AAA Cuts at: 4044 4277 4044 Size: 233 6740 DraIII CAC_nnn'GTG Cuts at: 2357 2357 Size: 6973 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5980 809 Size: 507 4664 1802 Fragments arranged by size: 4664 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4420 5309 6938 Size: 54 1425 50 275 18 2590 889 1629 Cuts at: 6938 8 Size: 43 Fragments arranged by size: 2590 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4420 1537 Size: 275 2608 4090 Fragments arranged by size: 4090 2608 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3452 4903 1353 Size: 2099 1451 3423 Fragments arranged by size: 3423 2099 1451 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5560 5934 6080 387 Size: 1064 138 3971 374 146 1280 Fragments arranged by size: 3971 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4131 4131 Size: 6973 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2208 2304 2663 2693 2841 2888 3356 Size: 393 576 96 359 30 148 47 468 Cuts at: 3356 3470 4038 5036 5633 6420 6688 1239 Size: 114 568 998 597 787 268 1524 Fragments arranged by size: 1524 998 787 597 576 568 468 393 359 268 148 114 96 47 30 EcoO109I rG'GnC_Cy Cuts at: 2231 2849 2231 Size: 618 6355 EcoRI G'AATT_C Cuts at: 2794 2917 4282 4660 2794 Size: 123 1365 378 5107 Fragments arranged by size: 5107 1365 378 123 EcoRV GAT'ATC Cuts at: 3833 3833 Size: 6973 FspI TGC'GCA Cuts at: 5479 5479 Size: 6973 HaeII r_GCGC'y Cuts at: 1678 6120 1678 Size: 4442 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2866 2898 5614 5646 5688 Size: 32 135 32 1294 32 2716 32 42 Cuts at: 5688 5720 1373 Size: 32 2626 Fragments arranged by size: 2716 2626 1294 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4317 4563 669 Size: 988 281 2379 246 3079 Fragments arranged by size: 3079 2379 988 281 246 HindIII A'AGCT_T Cuts at: 748 2241 3040 748 Size: 1493 799 4681 Fragments arranged by size: 4681 1493 799 HpaI GTT'AAC Cuts at: 4563 4563 Size: 6973 KpnI G_GTAC'C Cuts at: 4298 4298 Size: 6973 MfeI C'AATT_G Cuts at: 4572 4572 Size: 6973 MluI A'CGCG_T Cuts at: 6849 6849 Size: 6973 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6087 6271 1305 Size: 4782 184 2007 Fragments arranged by size: 4782 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5151 Fragments arranged by size: 5151 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2175 3542 4931 5290 5449 518 Size: 648 1009 1367 1389 359 159 2042 Fragments arranged by size: 2042 1389 1367 1009 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3811 4389 5066 6214 6459 1606 Size: 2205 578 677 1148 245 2120 Fragments arranged by size: 2205 2120 1148 677 578 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6421 NdeI CA'TA_TG Cuts at: 387 387 Size: 6973 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6973 NotI GC'GGCC_GC Cuts at: 4420 4420 Size: 6973 NspI r_CATG'y Cuts at: 1132 1229 2174 4331 5876 1132 Size: 97 945 2157 1545 2229 Fragments arranged by size: 2229 2157 1545 945 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6973 PciI A'CATG_T Cuts at: 5872 5872 Size: 6973 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6973 PfoI T'CCnGG_A Cuts at: 3255 3255 Size: 6973 PmeI GTTT'AAAC Cuts at: 4277 4277 Size: 6973 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5042 5074 6555 6587 5042 Size: 32 1481 32 5428 Fragments arranged by size: 5428 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2231 2849 2231 Size: 618 6355 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6973 PsiI TTA'TAA Cuts at: 3729 4208 4543 3729 Size: 479 335 6159 Fragments arranged by size: 6159 479 335 PstI C_TGCA'G Cuts at: 830 4325 830 Size: 3495 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6973 PvuII CAG'CTG Cuts at: 3811 3811 Size: 6973 SacI G_AGCT'C Cuts at: 4133 4133 Size: 6973 SalI G'TCGA_C Cuts at: 1936 4315 1936 Size: 2379 4594 SapI GCTCTTCn'nnn_ Cuts at: 3452 3452 Size: 6973 SbfI CC_TGCA'GG Cuts at: 4325 4325 Size: 6973 ScaI AGT'ACT Cuts at: 5221 6933 5221 Size: 1712 5261 SfcI C'TryA_G Cuts at: 826 1045 4094 4321 5456 6137 6328 826 Size: 219 3049 227 1135 681 191 1471 Fragments arranged by size: 3049 1471 1135 681 227 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6973 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6973 SmaI CCC'GGG Cuts at: 1872 4300 1872 Size: 2428 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4069 5077 5978 6240 820 Size: 197 926 2126 1008 901 262 1553 Fragments arranged by size: 2126 1553 1008 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4764 493 Size: 4271 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6973 SphI G_CATG'C Cuts at: 1132 4331 1132 Size: 3199 3774 SspI AAT'ATT Cuts at: 5 52 2347 4897 5 Size: 47 2295 2550 2081 Fragments arranged by size: 2550 2295 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6973 StyI C'CwwG_G Cuts at: 513 1065 2265 2484 4412 513 Size: 552 1200 219 1928 3074 Fragments arranged by size: 3074 1928 1200 552 219 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3348 3572 5000 5017 5170 5355 5800 Size: 1255 1768 224 1428 17 153 185 445 Cuts at: 5800 325 Size: 1498 Fragments arranged by size: 1768 1498 1428 1255 445 224 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5219 6931 96 Size: 274 80 33 51 1443 3242 1712 138 Fragments arranged by size: 3242 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6933 6965 6933 Size: 32 6941 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6973 XbaI T'CTAG_A Cuts at: 3568 4309 3568 Size: 741 6232 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6973 XmaI C'CCGG_G Cuts at: 1870 4298 1870 Size: 2428 4545 XmnI GAAnn'nnTTC Cuts at: 3033 5102 3033 Size: 2069 4904 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3963 4781 276 Size: 53 83 186 936 2429 818 2468 Fragments arranged by size: 2468 2429 936 818 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI