Restriction map for
pFN21AE9623
(Linear) (Six-base) MAPSORT of: KIEE9623.seq Check: 9756 from: 1 to: 2268
KIEE9623.orf
With 182 enzymes: SgfI *
March 22, 2012 18:35 ..
AatII G_ACGT'C
Cuts at: 0 1960 2268
Size: 1960 308
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 299 658 883 1351 1465 2268
Size: 203 96 359 225 468 114 803
Fragments arranged by size:
803 468 359 225 203 114 96
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 599 2268
Size: 599 1669
AlwNI CAG_nnn'CTG
Cuts at: 0 1488 2268
Size: 1488 780
ApoI r'AATT_y
Cuts at: 0 358 789 912 1237 1836 1915 2268
Size: 358 431 123 325 599 79 353
Fragments arranged by size:
599 431 358 353 325 123 79
BamHI G'GATC_C
Cuts at: 0 102 1582 2268
Size: 102 1480 686
Fragments arranged by size:
1480 686 102
BanII G_rGCy'C
Cuts at: 0 1093 2128 2268
Size: 1093 1035 140
Fragments arranged by size:
1093 1035 140
BbsI GAAGACnn'nnnn_
Cuts at: 0 814 2268
Size: 814 1454
BbvCI CC'TCA_GC
Cuts at: 0 1156 1777 2268
Size: 1156 621 491
Fragments arranged by size:
1156 621 491
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1810 1844 2268
Size: 1810 34 424
Fragments arranged by size:
1810 424 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1248 1779 2268
Size: 1248 531 489
Fragments arranged by size:
1248 531 489
BclI T'GATC_A
Cuts at: 0 174 2268
Size: 174 2094
Bme1580I G_kGCm'C
Cuts at: 0 595 2268
Size: 595 1673
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1346 2268
Size: 1346 922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 836 2033 2268
Size: 688 148 1197 235
Fragments arranged by size:
1197 688 235 148
Bpu10I CC'TnA_GC
Cuts at: 0 1156 1777 2268
Size: 1156 621 491
Fragments arranged by size:
1156 621 491
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2085 2268
Size: 2085 183
BsaHI Gr'CG_yC
Cuts at: 0 1957 2268
Size: 1957 311
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1765 2268
Size: 1765 503
BseYI C'CCAG_C
Cuts at: 0 402 2268
Size: 402 1866
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1657 1754 2268
Size: 1657 97 514
Fragments arranged by size:
1657 514 97
BsiHKAI G_wGCw'C
Cuts at: 0 938 2128 2268
Size: 938 1190 140
Fragments arranged by size:
1190 938 140
BsmI GAATG_Cn'
Cuts at: 0 695 1309 2268
Size: 695 614 959
Fragments arranged by size:
959 695 614
Bsp1286I G_dGCh'C
Cuts at: 0 595 938 1093 2128 2268
Size: 595 343 155 1035 140
Fragments arranged by size:
1035 595 343 155 140
BsrDI GCAATG_nn'
Cuts at: 0 515 571 1062 1646 2268
Size: 515 56 491 584 622
Fragments arranged by size:
622 584 515 491 56
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2252 2268
Size: 2252 16
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1251 2268
Size: 1251 1017
BstYI r'GATC_y
Cuts at: 0 102 1582 1757 2268
Size: 102 1480 175 511
Fragments arranged by size:
1480 511 175 102
BtsI GCAGTG_nn'
Cuts at: 0 212 2268
Size: 212 2056
DraI TTT'AAA
Cuts at: 0 2039 2268
Size: 2039 229
DraIII CAC_nnn'GTG
Cuts at: 0 352 2268
Size: 352 1916
EarI CTCTTCn'nnn_
Cuts at: 0 1447 2268
Size: 1447 821
EcoICRI GAG'CTC
Cuts at: 0 2126 2268
Size: 2126 142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 299 658 688 836 883 1351 1465
Size: 203 96 359 30 148 47 468 114
Cuts at: 1465 2033 2268
Size: 568 235
Fragments arranged by size:
568 468 359 235 203 148 114 96
47 30
EcoO109I rG'GnC_Cy
Cuts at: 0 226 844 2268
Size: 226 618 1424
Fragments arranged by size:
1424 618 226
EcoRI G'AATT_C
Cuts at: 0 789 912 2268
Size: 789 123 1356
Fragments arranged by size:
1356 789 123
EcoRV GAT'ATC
Cuts at: 0 1828 2268
Size: 1828 440
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 861 893 2268
Size: 861 32 1375
Fragments arranged by size:
1375 861 32
HindIII A'AGCT_T
Cuts at: 0 236 1035 2268
Size: 236 799 1233
Fragments arranged by size:
1233 799 236
MslI CAynn'nnrTG
Cuts at: 0 170 1537 2268
Size: 170 1367 731
Fragments arranged by size:
1367 731 170
MspA1I CmG'CkG
Cuts at: 0 1806 2268
Size: 1806 462
NspI r_CATG'y
Cuts at: 0 169 2268
Size: 169 2099
PfoI T'CCnGG_A
Cuts at: 0 1250 2268
Size: 1250 1018
PpuMI rG'GwC_Cy
Cuts at: 0 226 844 2268
Size: 226 618 1424
Fragments arranged by size:
1424 618 226
PsiI TTA'TAA
Cuts at: 0 1724 2203 2268
Size: 1724 479 65
Fragments arranged by size:
1724 479 65
PvuII CAG'CTG
Cuts at: 0 1806 2268
Size: 1806 462
SacI G_AGCT'C
Cuts at: 0 2128 2268
Size: 2128 140
SapI GCTCTTCn'nnn_
Cuts at: 0 1447 2268
Size: 1447 821
SfcI C'TryA_G
Cuts at: 0 2089 2268
Size: 2089 179
SmlI C'TyrA_G
Cuts at: 0 2064 2268
Size: 2064 204
SspI AAT'ATT
Cuts at: 0 342 2268
Size: 342 1926
StyI C'CwwG_G
Cuts at: 0 260 479 2268
Size: 260 219 1789
Fragments arranged by size:
1789 260 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1343 1567 2268
Size: 1343 224 701
Fragments arranged by size:
1343 701 224
XbaI T'CTAG_A
Cuts at: 0 1563 2268
Size: 1563 705
XmnI GAAnn'nnTTC
Cuts at: 0 1028 2268
Size: 1028 1240
ZraI GAC'GTC
Cuts at: 0 1958 2268
Size: 1958 310
Enzymes that do cut:
AatII AcuI AlfI AlwNI ApoI BamHI BanII BbsI
BbvCI BcgI BciVI BclI Bme1580I BplI BpmI Bpu10I
BpuEI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BsrDI BstAPI BstXI BstYI BtsI DraI DraIII EarI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV Hin4I HindIII MslI
MspA1I NspI PfoI PpuMI PsiI PvuII SacI SapI
SfcI SmlI SspI StyI TaqII XbaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI BsaI BsaAI BsaBI BsaWI
BsaXI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DrdI EaeI EagI EciI
EcoNI FalI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TatI TstI
Tth111I XcmI XhoI XmaI