Restriction map for
pFN21AE1228
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII AccI AccIII AclI AcyI
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI AspEI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BbvI BclI BcnI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HpaI
HphI KasI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MspA1I MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI TseI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 168 GGTACC G^GTACC
AccII 832 CGCG CG^CG
AfaI 169 GTAC GT^AC
AflII 85 CTTAAG C^TTAAG
AluI 54 AGCT AG^CT
Alw44I 304 GTGCAC G^TGCAC
AlwNI 575 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 304 GTGCAC G^TGCAC
Asp718I 168 GGTACC G^GTACC
BanI 168 GGTACC G^G[CT][GA]CC
BcgI 332 GCATCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 850 CTAG C^TAG
BfrI 85 CTTAAG C^TTAAG
BglI 806 GCCGTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 612 AGATCT A^GATCT
BsaJI 879 CCAAGG C^C[ACGT][ACGT]GG
BsrDI 209 GCAATG GCAATG(2/0)
BssKI 114 CCTGG ^CC[ACGT]GG
BstF5I 777 GGATG GGATG(2/0)
BstNI 114 CCTGG CC^[AT]GG
BstUI 832 CGCG CG^CG
BstXI 515 CCAAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 612 AGATCT [GA]^GATC[CT]
DraII 467 GGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 160 TGGCCG [CT]^GGCC[GA]
EarI 406 GAAGAG CTCTTC(1/4)
EcoNI 844 CCTTTACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 467 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRII 114 CCTGG ^CC[AT]GG
EcoT14I 879 CCAAGG C^C[AT][AT]GG
FauI 932 CCCGC CCCGC(4/6)
FokI 777 GGATG GGATG(9/13)
HapII 163 CCGG C^CGG
HgaI 833 GCGTC GACGC(5/10)
HindIII 53 AAGCTT A^AGCTT
HpaII 163 CCGG C^CGG
KpnI 168 GGTACC GGTAC^C
Ksp632I 406 GAAGAG CTCTTC(1/4)
MaeI 850 CTAG C^TAG
MaeII 539 ACGT A^CGT
MflI 612 AGATCT [GA]^GATC[CT]
MspI 163 CCGG C^CGG
MvaI 114 CCTGG CC^[AT]GG
MvnI 832 CGCG CG^CG
NspI 935 GCATGC [GA]CATG^[CT]
PflMI 545 CCATGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 890 GAGTC GAGTC(4/5)
PpuMI 467 GGGTCCC [GA]G^G[AT]CC[CT]
RsaI 169 GTAC GT^AC
ScrFI 114 CCTGG CC^[ACGT]GG
SfaNI 332 GCATC GCATC(5/9)
SfcI 204 CTACAG C^T[GA][CT]AG
SphI 935 GCATGC GCATG^C
SspI 264 AATATT AAT^ATT
StyI 879 CCAAGG C^C[AT][AT]GG
TaqI 810 TCGA T^CGA
TthHB8I 810 TCGA T^CGA
Van91I 545 CCATGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 612 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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Asp700I 610 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
721 GAAGAACTTC
AspHI 193 GAGCAC G[AT]GC[AT]^C
304 GTGCAC
AvaII 468 GGTCC G^G[AT]CC
669 GGTCC
BciVI 322 GGATAC GTATCC(6/5)
110 GTATCC
BmyI 193 GAGCAC G[AGT]GC[ACT]^C
304 GTGCAC
BpmI 14 CTCCAG CTGGAG(16/14)
523 CTGGAG
BseRI 598 GAGGAG GAGGAG(10/8)
601 GAGGAG
BsiHKAI 193 GAGCAC G[AT]GC[AT]^C
304 GTGCAC
BsmFI 469 GTCCC GGGAC(10/14)
670 GTCCC
Bsp1286I 193 GAGCAC G[AGT]GC[ACT]^C
304 GTGCAC
DdeI 371 CTCAG C^T[ACGT]AG
581 CTGAG
DraI 58 TTTAAA TTT^AAA
313 TTTAAA
Fnu4HI 827 GCCGC GC^[ACGT]GC
830 GCCGC
ItaI 827 GCCGC GC^[ACGT]GC
830 GCCGC
SmlI 85 CTTAAG C^T[CT][GA]AG
674 CTCAAG
TfiI 9 GAATC G^A[AT]TC
384 GATTC
XmnI 610 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
721 GAAGAACTTC
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AcsI AlwI ApoI BbsI
BpuAI BsiYI BslI BsrI Cac8I
Cfr13I DpnII HaeIII HinfI MaeIII
MboI MboII MnlI MseI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
Tru9I Tsp45I Tsp509I TspRI