################################## # NO CUT ENZYMES ( 202 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SapI Sau96I SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 170 GTCGAC GT^[AC][GT]AC AccII 226 CGCG CG^CG AciI 227 GCGG CCGC(-3/-1) AfaI 157 GTAC GT^AC AlwI 6 GATCC GGATC(4/5) BfaI 79 CTAG C^TAG BsiYI 175 CCAGCCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 175 CCAGCCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 230 GGGAC GGGAC(10/14) BsrI 219 CCAGT ACTGG(1/-1) BssKI 301 CCTGG ^CC[ACGT]GG BstAPI 268 GCAGTGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 184 GGTTACC G^GT[ACGT]ACC BstF5I 143 GGATG GGATG(2/0) BstNI 301 CCTGG CC^[AT]GG BstPI 184 GGTTACC G^GT[ACGT]ACC BstUI 226 CGCG CG^CG DpnII 6 GATC ^GATC EarI 127 GAAGAG CTCTTC(1/4) EcoO65I 184 GGTTACC G^GT[ACGT]ACC EcoRII 301 CCTGG ^CC[AT]GG FauI 227 GCGGG CCCGC(4/6) FokI 143 GGATG GGATG(9/13) HincII 170 GTCGAC GT[CT]^[GA]AC HindII 170 GTCGAC GT[CT]^[GA]AC HinfI 132 GAATC G^A[ACGT]TC HphI 3 GGTGA GGTGA(8/7) Ksp632I 127 GAAGAG CTCTTC(1/4) MaeI 79 CTAG C^TAG MaeIII 185 GTTAC ^GT[ACGT]AC MboI 6 GATC ^GATC MseI 47 TTAA T^TAA MvaI 301 CCTGG CC^[AT]GG MvnI 226 CGCG CG^CG NdeII 6 GATC ^GATC RsaI 157 GTAC GT^AC SalI 170 GTCGAC G^TCGAC Sau3AI 6 GATC ^GATC ScrFI 301 CCTGG CC^[ACGT]GG SexAI 300 ACCTGGT A^CC[AT]GGT SfcI 38 CTACAG C^T[GA][CT]AG TaqI 171 TCGA T^CGA TfiI 132 GAATC G^A[AT]TC Tru9I 47 TTAA T^TAA TspRI 269 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 171 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### BbvI 118 GCAGC GCAGC(8/12) 333 GCAGC Cac8I 324 GCAAGC GC[ACGT]^[ACGT]GC 336 GCAAGC DdeI 200 CTCAG C^T[ACGT]AG 282 CTTAG Fnu4HI 118 GCAGC GC^[ACGT]GC 333 GCAGC ItaI 118 GCAGC GC^[ACGT]GC 333 GCAGC MnlI 29 CCTC CCTC(7/6) 199 CCTC SfaNI 238 GATGC GCATC(5/9) 22 GCATC TseI 118 GCAGC G^C[AT]GC 333 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### MboII MwoI