################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BanI BbeI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BseAI BsgI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HindIII HpaI KasI KpnI KspI MamI MluI MroI MseI MslI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccII 21 CGCG CG^CG AcyI 260 GACGCC G[GA]^CG[CT]C AluI 69 AGCT AG^CT AvaI 47 CTCGGG C^[CT]CG[GA]G BamHI 243 GGATCC G^GATCC BanII 44 GGGCTC G[GA]GC[CT]^C BbrPI 412 CACGTG CAC^GTG BpmI 71 CTGGAG CTGGAG(16/14) BsaAI 412 CACGTG [CT]AC^GT[GA] BsaHI 260 GACGCC G[GA]^CG[CT]C BsaWI 157 TCCGGT [AT]^CCGG[AT] BsiEI 159 CGGTCG CG[GA][CT]^CG BsiYI 58 CCTAGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 58 CCTAGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 258 GAGAC GTCTC(1/5) BsmBI 258 GAGACG CGTCTC(1/5) BsmFI 492 GTCCC GGGAC(10/14) BsoBI 47 CTCGGG C^[CT]CG[GA]G BspMI 277 ACCTGC ACCTGC(4/8) BssSI 302 CTCGTG CACGAG(-5/-1) BstF5I 52 GGATG GGATG(2/0) BstUI 21 CGCG CG^CG BstYI 243 GGATCC [GA]^GATC[CT] CfoI 374 GCGC GCG^C DdeI 170 CTAAG C^T[ACGT]AG DsaI 93 CCATGG C^C[GA][CT]GG EarI 80 GAAGAG CTCTTC(1/4) EcoNI 58 CCTAGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 52 GGATG GGATG(9/13) HapII 158 CCGG C^CGG HgaI 260 GACGC GACGC(5/10) HhaI 374 GCGC GCG^C Hin1I 260 GACGCC G[GA]^CG[CT]C HinP1I 374 GCGC G^CGC HincII 446 GTCAAC GT[CT]^[GA]AC HindII 446 GTCAAC GT[CT]^[GA]AC HinfI 154 GAGTC G^A[ACGT]TC HpaII 158 CCGG C^CGG HphI 151 GGTGA GGTGA(8/7) Ksp632I 80 GAAGAG CTCTTC(1/4) MaeII 413 ACGT A^CGT MfeI 423 CAATTG C^AATTG MflI 243 GGATCC [GA]^GATC[CT] MspA1I 457 CCGCTG C[AC]G^C[GT]G MspI 158 CCGG C^CGG MunI 423 CAATTG C^AATTG MvnI 21 CGCG CG^CG NcoI 93 CCATGG C^CATGG NlaIII 94 CATG CATG^ PleI 154 GAGTC GAGTC(4/5) PmaCI 412 CACGTG CAC^GTG PmlI 412 CACGTG CAC^GTG Tsp45I 305 GTGAC ^GT[GC]AC Tsp509I 424 AATT ^AATT TspRI 268 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 243 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwI 244 GATCC GGATC(4/5) 243 GGATC BbvI 428 GCAGC GCAGC(8/12) 459 GCTGC BmyI 44 GGGCTC G[AGT]GC[ACT]^C 355 GGGCAC BseRI 62 GAGGAG GAGGAG(10/8) 65 GAGGAG Bsp1286I 44 GGGCTC G[AGT]GC[ACT]^C 355 GGGCAC BssKI 378 CCAGG ^CC[ACGT]GG 442 CCAGG BstNI 378 CCAGG CC^[AT]GG 442 CCAGG DpnII 244 GATC ^GATC 313 GATC EcoRII 378 CCAGG ^CC[AT]GG 442 CCAGG EcoT14I 93 CCATGG C^C[AT][AT]GG 483 CCAAGG MboI 244 GATC ^GATC 313 GATC MboII 80 GAAGA GAAGA(8/7) 17 TCTTC MvaI 378 CCAGG CC^[AT]GG 442 CCAGG NdeII 244 GATC ^GATC 313 GATC NlaIV 115 GGTTCC GG[ACGT]^[ACGT]CC 243 GGATCC Sau3AI 244 GATC ^GATC 313 GATC ScrFI 378 CCAGG CC^[ACGT]GG 442 CCAGG SfaNI 53 GATGC GCATC(5/9) 395 GATGC StyI 93 CCATGG C^C[AT][AT]GG 483 CCAAGG TseI 428 GCAGC G^C[AT]GC 459 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AciI BalI BfaI BsaJI EaeI Fnu4HI HaeIII ItaI MaeI MaeIII MluNI MnlI MscI MwoI