Restriction map for
pFN21AB7580
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaII AvrII BalI
BbrPI BbvCI BcgI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV FseI HindIII KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 236 CGCG CG^CG
AcsI 571 GAATTC [GA]^AATT[CT]
AcyI 424 GGCGCC G[GA]^CG[CT]C
ApaI 348 GGGCCC GGGCC^C
ApoI 571 GAATTC [GA]^AATT[CT]
AvaI 42 CCCGGG C^[CT]CG[GA]G
AviII 479 TGCGCA TGC^GCA
BamHI 186 GGATCC G^GATCC
BbeI 424 GGCGCC GGCGC^C
BbsI 241 GAAGAC GAAGAC(2/6)
BpmI 246 CTGGAG CTGGAG(16/14)
BpuAI 241 GAAGAC GAAGAC(2/6)
BsaHI 424 GGCGCC G[GA]^CG[CT]C
BsgI 490 GTGCAG GTGCAG(16/14)
BsiEI 329 CGACCG CG[GA][CT]^CG
BsmBI 200 CGTCTC CGTCTC(1/5)
BsoBI 42 CCCGGG C^[CT]CG[GA]G
BspHI 53 TCATGA T^CATGA
BsrDI 577 CATTGC GCAATG(2/0)
BstUI 236 CGCG CG^CG
BstYI 186 GGATCC [GA]^GATC[CT]
DraII 348 GGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 196 TGGCCG [CT]^GGCC[GA]
EcoO109I 348 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 571 GAATTC G^AATTC
EcoT22I 470 ATGCAT ATGCA^T
FspI 479 TGCGCA TGC^GCA
HaeII 424 GGCGCC [GA]GCGC^[CT]
HgaI 418 GACGC GACGC(5/10)
Hin1I 424 GGCGCC G[GA]^CG[CT]C
HincII 622 GTTAAC GT[CT]^[GA]AC
HindII 622 GTTAAC GT[CT]^[GA]AC
HpaI 622 GTTAAC GTT^AAC
KasI 424 GGCGCC G^GCGCC
MaeII 299 ACGT A^CGT
MboII 241 GAAGA GAAGA(8/7)
MflI 186 GGATCC [GA]^GATC[CT]
MseI 623 TTAA T^TAA
MslI 48 CACTCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 77 CAGCTG C[AC]G^C[GT]G
MvnI 236 CGCG CG^CG
NarI 424 GGCGCC GG^CGCC
NdeI 172 CATATG CA^TATG
NsiI 470 ATGCAT ATGCA^T
NspI 35 GCATGT [GA]CATG^[CT]
PvuII 77 CAGCTG CAG^CTG
RcaI 53 TCATGA T^CATGA
SfoI 424 GGCGCC GGC^GCC
SmaI 42 CCCGGG CCC^GGG
TaqI 328 TCGA T^CGA
TfiI 666 GAATC G^A[AT]TC
Tru9I 623 TTAA T^TAA
TthHB8I 328 TCGA T^CGA
XhoII 186 GGATCC [GA]^GATC[CT]
XmaCI 42 CCCGGG C^CCGGG
XmaI 42 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 187 GATCC GGATC(4/5)
186 GGATC
BanI 138 GGCACC G^G[CT][GA]CC
424 GGCGCC
BciVI 165 GTATCC GTATCC(6/5)
255 GTATCC
BclI 543 TGATCA T^GATCA
692 TGATCA
BcnI 42 CCCGG CC^[GC]GG
43 CCGGG
BspMI 492 GCAGGT ACCTGC(4/8)
834 GCAGGT
BsrFI 141 ACCGGC [GA]^CCGG[CT]
288 GCCGGT
BstF5I 597 CATCC GGATG(2/0)
476 GGATG
CfoI 425 GCGC GCG^C
480 GCGC
Cfr10I 141 ACCGGC [GA]^CCGG[CT]
288 GCCGGT
EcoT14I 431 CCAAGG C^C[AT][AT]GG
677 CCAAGG
FbaI 543 TGATCA T^GATCA
692 TGATCA
FokI 597 CATCC GGATG(9/13)
476 GGATG
HhaI 425 GCGC GCG^C
480 GCGC
HinP1I 425 GCGC G^CGC
480 GCGC
NciI 42 CCCGG CC^[GC]GG
43 CCGGG
SfaNI 114 GATGC GCATC(5/9)
477 GATGC
SfcI 225 CTACAG C^T[GA][CT]AG
336 CTACAG
StyI 431 CCAAGG C^C[AT][AT]GG
677 CCAAGG
Tsp509I 127 AATT ^AATT
572 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI BanII BbvI
BfaI BglI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstNI Cac8I Cfr13I DpnII
EcoRII FauI Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI TseI
Tsp45I TspRI