(Circular) (Six-base) MAPSORT of: pFN21AB7580.seq Check: 8115 from: 1 to: 5557 pFN21AB7580 5557 bp With 182 enzymes: SgfI * March 28, 2012 12:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2488 2488 Size: 5557 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3367 278 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2900 1937 Size: 963 4594 Acc65I G'GTAC_C Cuts at: 2878 2878 Size: 5557 AclI AA'CG_TT Cuts at: 1563 3684 4057 1563 Size: 2121 373 3063 Fragments arranged by size: 3063 2121 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3620 5004 5272 1632 Size: 1988 1384 268 1917 Fragments arranged by size: 1988 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5360 AflIII A'CryG_T Cuts at: 1203 4456 5433 1203 Size: 3253 977 1327 Fragments arranged by size: 3253 1327 977 AgeI A'CCGG_T Cuts at: 4388 4388 Size: 5557 AhdI GACnn_n'nnGTC Cuts at: 4286 4286 Size: 5557 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2510 1184 Size: 1326 4231 AlwNI CAG_nnn'CTG Cuts at: 3300 4872 3300 Size: 1572 3985 ApaI G_GGCC'C Cuts at: 2357 2357 Size: 5557 ApaLI G'TGCA_C Cuts at: 3614 4770 3614 Size: 1156 4401 ApoI r'AATT_y Cuts at: 1493 2576 2866 3092 3244 5353 1493 Size: 1083 290 226 152 2109 1697 Fragments arranged by size: 2109 1697 1083 290 226 152 AseI AT'TA_AT Cuts at: 160 1032 4111 160 Size: 872 3079 1606 Fragments arranged by size: 3079 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 AvaI C'yCGr_G Cuts at: 1870 1943 2047 2882 1870 Size: 73 104 835 4545 Fragments arranged by size: 4545 835 104 73 BamHI G'GATC_C Cuts at: 2191 2887 3254 2191 Size: 696 367 4494 Fragments arranged by size: 4494 696 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2143 2429 2878 4333 618 Size: 325 217 627 356 286 449 1455 1842 Fragments arranged by size: 1842 1455 627 449 356 325 286 217 BanII G_rGCy'C Cuts at: 1391 2066 2225 2357 2876 1391 Size: 675 159 132 519 4072 Fragments arranged by size: 4072 675 519 159 132 BbeI G_GCGC'C Cuts at: 2433 2433 Size: 5557 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2253 928 Size: 971 354 4232 Fragments arranged by size: 4232 971 354 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3748 3782 1903 Size: 34 1811 34 3678 Fragments arranged by size: 3678 1811 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2181 2271 3449 4659 2181 Size: 90 1178 1210 3079 Fragments arranged by size: 3079 1210 1178 90 BclI T'GATC_A Cuts at: 1548 2548 2697 1548 Size: 1000 149 4408 Fragments arranged by size: 4408 1000 149 BfrBI ATG'CAT Cuts at: 2477 2477 Size: 5557 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2070 2361 2391 Size: 107 122 71 631 605 398 291 30 Cuts at: 2391 2400 2438 4168 136 Size: 9 38 1730 1525 Fragments arranged by size: 1730 1525 631 605 398 291 122 107 71 38 30 9 BglII A'GATC_T Cuts at: 5552 5552 Size: 5557 BlpI GC'TnA_GC Cuts at: 2974 2974 Size: 5557 Bme1580I G_kGCm'C Cuts at: 1790 2054 2288 2357 3618 4774 1790 Size: 264 234 69 1261 1156 2573 Fragments arranged by size: 2573 1261 1156 264 234 69 BmgBI CAC'GTC Cuts at: 1318 2305 1318 Size: 987 4570 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2710 3288 4246 Size: 416 53 83 399 24 1293 578 958 Cuts at: 4246 442 Size: 1753 Fragments arranged by size: 1753 1293 958 578 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5557 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2272 4217 1239 Size: 1033 1945 2579 Fragments arranged by size: 2579 1945 1033 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3682 4547 4845 3682 Size: 865 298 4394 Fragments arranged by size: 4394 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4220 882 Size: 617 2721 2219 Fragments arranged by size: 2721 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3348 493 Size: 711 2144 2702 Fragments arranged by size: 2702 2144 711 BsaBI GATnn'nnATC Cuts at: 5551 5551 Size: 5557 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2430 3364 3746 4381 Size: 53 83 186 936 897 934 382 635 Cuts at: 4381 275 Size: 1451 Fragments arranged by size: 1451 936 934 897 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3990 4388 4662 4809 5220 3990 Size: 398 274 147 411 4327 Fragments arranged by size: 4327 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2724 2741 2754 2771 1178 Size: 30 165 30 1321 17 13 17 3964 Fragments arranged by size: 3964 1321 165 30 30 17 17 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5557 BseYI C'CCAG_C Cuts at: 2084 2183 2450 4760 5256 2084 Size: 99 267 2310 496 2385 Fragments arranged by size: 2385 2310 496 267 99 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2516 1163 Size: 573 780 4204 Fragments arranged by size: 4204 780 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2337 3007 3768 4796 1540 Size: 275 186 336 670 761 1028 2301 Fragments arranged by size: 2301 1028 761 670 336 275 186 BsiHKAI G_wGCw'C Cuts at: 3618 3703 4774 3618 Size: 85 1071 4401 Fragments arranged by size: 4401 1071 85 BsmI GAATG_Cn' Cuts at: 3068 3161 3068 Size: 93 5464 BsmBI CGTCTCn'nnnn_ Cuts at: 2211 2211 Size: 5557 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2066 2225 2288 2357 2876 3618 Size: 399 264 12 159 63 69 519 742 Cuts at: 3618 3703 4774 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 519 399 264 159 85 69 63 12 BspEI T'CCGG_A Cuts at: 5220 5220 Size: 5557 BspHI T'CATG_A Cuts at: 2058 3446 5176 2058 Size: 1388 1730 2439 Fragments arranged by size: 2439 1730 1388 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2488 2830 2912 844 Size: 1644 342 82 3489 Fragments arranged by size: 3489 1644 342 82 BsrBI CCG'CTC Cuts at: 1394 1823 3444 1394 Size: 429 1621 3507 Fragments arranged by size: 3507 1621 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2581 4052 4226 65 Size: 1183 1333 1471 174 1396 Fragments arranged by size: 1471 1396 1333 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2146 2293 4201 4388 1700 Size: 110 336 147 1908 187 2869 Fragments arranged by size: 2869 1908 336 187 147 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5557 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5557 BssSI C'ACGA_G Cuts at: 3617 4629 3617 Size: 1012 4545 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5557 BstBI TT'CG_AA Cuts at: 4361 4361 Size: 5557 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5557 BstYI r'GATC_y Cuts at: 1972 2191 2887 3254 3639 3656 5108 5194 5206 Size: 219 696 367 385 17 1452 86 12 Cuts at: 5206 5552 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 696 385 367 346 219 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5005 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4818 Fragments arranged by size: 4818 683 56 BtsI GCAGTG_nn' Cuts at: 3073 3867 3887 3073 Size: 794 20 4743 Fragments arranged by size: 4743 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5557 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5522 DraI TTT'AAA Cuts at: 2861 2861 Size: 5557 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4564 809 Size: 507 3248 1802 Fragments arranged by size: 3248 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2201 3004 3893 Size: 54 1425 50 275 18 371 803 889 Cuts at: 3893 5522 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 803 371 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3004 1537 Size: 275 1192 4090 Fragments arranged by size: 4090 1192 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3487 1353 Size: 2134 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4144 4518 4664 387 Size: 1064 138 2555 374 146 1280 Fragments arranged by size: 2555 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2272 3620 4217 5004 5272 1239 Size: 393 640 1348 597 787 268 1524 Fragments arranged by size: 1524 1348 787 640 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2354 2354 Size: 5557 EcoRI G'AATT_C Cuts at: 2576 2866 3244 2576 Size: 290 378 4889 Fragments arranged by size: 4889 378 290 FspI TGC'GCA Cuts at: 2486 4063 2486 Size: 1577 3980 HaeII r_GCGC'y Cuts at: 1678 2433 4704 1678 Size: 755 2271 2531 Fragments arranged by size: 2531 2271 755 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2245 2277 2294 2326 2741 Size: 32 135 32 673 32 17 32 415 Cuts at: 2741 2773 4198 4230 4272 4304 1373 Size: 32 1425 32 42 32 2626 Fragments arranged by size: 2626 1425 673 415 135 42 32 32 32 32 32 32 32 17 HincII GTy'rAC Cuts at: 669 1657 1938 2629 2901 3147 669 Size: 988 281 691 272 246 3079 Fragments arranged by size: 3079 988 691 281 272 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5557 HpaI GTT'AAC Cuts at: 2629 3147 2629 Size: 518 5039 KasI G'GCGC_C Cuts at: 2429 2429 Size: 5557 KpnI G_GTAC'C Cuts at: 2882 2882 Size: 5557 MfeI C'AATT_G Cuts at: 3156 3156 Size: 5557 MluI A'CGCG_T Cuts at: 5433 5433 Size: 5557 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4671 4855 1305 Size: 3366 184 2007 Fragments arranged by size: 3366 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3735 Fragments arranged by size: 3735 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 3515 3874 4033 518 Size: 648 891 1458 359 159 2042 Fragments arranged by size: 2042 1458 891 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2084 2973 3650 4798 5043 1606 Size: 478 889 677 1148 245 2120 Fragments arranged by size: 2120 1148 889 677 478 245 NarI GG'CG_CC Cuts at: 2430 2430 Size: 5557 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5005 NdeI CA'TA_TG Cuts at: 387 2178 387 Size: 1791 3766 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5557 NotI GC'GGCC_GC Cuts at: 3004 3004 Size: 5557 NsiI A_TGCA'T Cuts at: 2479 2479 Size: 5557 NspI r_CATG'y Cuts at: 1132 1229 2044 2915 4460 1132 Size: 97 815 871 1545 2229 Fragments arranged by size: 2229 1545 871 815 97 PasI CC'CwG_GG Cuts at: 1348 2066 2768 1348 Size: 718 702 4137 Fragments arranged by size: 4137 718 702 PciI A'CATG_T Cuts at: 4456 4456 Size: 5557 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5557 PmeI GTTT'AAAC Cuts at: 2861 2861 Size: 5557 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3626 3658 5139 5171 3626 Size: 32 1481 32 4012 Fragments arranged by size: 4012 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5557 PsiI TTA'TAA Cuts at: 3127 3127 Size: 5557 PspOMI G'GGCC_C Cuts at: 2353 2353 Size: 5557 PstI C_TGCA'G Cuts at: 830 2909 830 Size: 2079 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5557 PvuII CAG'CTG Cuts at: 2084 2084 Size: 5557 SalI G'TCGA_C Cuts at: 1936 2899 1936 Size: 963 4594 SbfI CC_TGCA'GG Cuts at: 2909 2909 Size: 5557 ScaI AGT'ACT Cuts at: 3805 5517 3805 Size: 1712 3845 SfcI C'TryA_G Cuts at: 826 1045 2230 2341 2905 4040 4721 4912 826 Size: 219 1185 111 564 1135 681 191 1471 Fragments arranged by size: 1471 1185 1135 681 564 219 191 111 SfoI GGC'GCC Cuts at: 2431 2431 Size: 5557 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5557 SmaI CCC'GGG Cuts at: 1872 2049 2884 1872 Size: 177 835 4545 Fragments arranged by size: 4545 835 177 SmlI C'TyrA_G Cuts at: 820 1017 1943 3661 4562 4824 820 Size: 197 926 1718 901 262 1553 Fragments arranged by size: 1718 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3348 493 Size: 2855 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5557 SphI G_CATG'C Cuts at: 1132 2915 1132 Size: 1783 3774 SspI AAT'ATT Cuts at: 5 52 3481 5 Size: 47 3429 2081 Fragments arranged by size: 3429 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5557 StyI C'CwwG_G Cuts at: 513 1065 2436 2682 2996 513 Size: 552 1371 246 314 3074 Fragments arranged by size: 3074 1371 552 314 246 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2351 3584 3601 3754 3939 4384 325 Size: 1255 771 1233 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1233 771 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2276 3803 5515 Size: 274 80 33 51 1443 299 1527 1712 Cuts at: 5515 96 Size: 138 Fragments arranged by size: 1712 1527 1443 299 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2724 2756 5517 5549 2724 Size: 32 2761 32 2732 Fragments arranged by size: 2761 2732 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5557 XbaI T'CTAG_A Cuts at: 2893 2893 Size: 5557 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5557 XmaI C'CCGG_G Cuts at: 1870 2047 2882 1870 Size: 177 835 4545 Fragments arranged by size: 4545 835 177 XmnI GAAnn'nnTTC Cuts at: 3686 3686 Size: 5557 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3365 276 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI