(Linear) (Six-base) MAPSORT of: KIBB7580.seq Check: 4496 from: 1 to: 852 KIBB7580.orf With 182 enzymes: SgfI * March 22, 2012 15:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 483 852 Size: 483 369 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 505 852 Size: 505 347 ApaI G_GGCC'C Cuts at: 0 352 852 Size: 352 500 ApoI r'AATT_y Cuts at: 0 571 852 Size: 571 281 AvaI C'yCGr_G Cuts at: 0 42 852 Size: 42 810 BamHI G'GATC_C Cuts at: 0 186 852 Size: 186 666 BanI G'GyrC_C Cuts at: 0 138 424 852 Size: 138 286 428 Fragments arranged by size: 428 286 138 BanII G_rGCy'C Cuts at: 0 61 220 352 852 Size: 61 159 132 500 Fragments arranged by size: 500 159 132 61 BbeI G_GCGC'C Cuts at: 0 428 852 Size: 428 424 BbsI GAAGACnn'nnnn_ Cuts at: 0 248 852 Size: 248 604 BciVI GTATCCnnnnn_n' Cuts at: 0 176 266 852 Size: 176 90 586 Fragments arranged by size: 586 176 90 BclI T'GATC_A Cuts at: 0 543 692 852 Size: 543 149 160 Fragments arranged by size: 543 160 149 BfrBI ATG'CAT Cuts at: 0 472 852 Size: 472 380 BglI GCCn_nnn'nGGC Cuts at: 0 65 356 386 395 433 852 Size: 65 291 30 9 38 419 Fragments arranged by size: 419 291 65 38 30 9 Bme1580I G_kGCm'C Cuts at: 0 49 283 352 852 Size: 49 234 69 500 Fragments arranged by size: 500 234 69 49 BmgBI CAC'GTC Cuts at: 0 300 852 Size: 300 552 BmrI ACTGGGnnnn_n' Cuts at: 0 705 852 Size: 705 147 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 267 852 Size: 267 585 BsaHI Gr'CG_yC Cuts at: 0 425 852 Size: 425 427 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 719 736 749 766 852 Size: 719 17 13 17 86 Fragments arranged by size: 719 86 17 17 13 BseYI C'CCAG_C Cuts at: 0 79 178 445 852 Size: 79 99 267 407 Fragments arranged by size: 407 267 99 79 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 511 852 Size: 511 341 BsiEI CG_ry'CG Cuts at: 0 332 852 Size: 332 520 BsmBI CGTCTCn'nnnn_ Cuts at: 0 206 852 Size: 206 646 Bsp1286I G_dGCh'C Cuts at: 0 49 61 220 283 352 852 Size: 49 12 159 63 69 500 Fragments arranged by size: 500 159 69 63 49 12 BspHI T'CATG_A Cuts at: 0 53 852 Size: 53 799 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 483 825 852 Size: 483 342 27 Fragments arranged by size: 483 342 27 BsrDI GCAATG_nn' Cuts at: 0 576 852 Size: 576 276 BsrFI r'CCGG_y Cuts at: 0 141 288 852 Size: 141 147 564 Fragments arranged by size: 564 147 141 BstYI r'GATC_y Cuts at: 0 186 852 Size: 186 666 EaeI y'GGCC_r Cuts at: 0 196 852 Size: 196 656 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 267 852 Size: 267 585 EcoO109I rG'GnC_Cy Cuts at: 0 349 852 Size: 349 503 EcoRI G'AATT_C Cuts at: 0 571 852 Size: 571 281 FspI TGC'GCA Cuts at: 0 481 852 Size: 481 371 HaeII r_GCGC'y Cuts at: 0 428 852 Size: 428 424 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 240 272 289 321 736 768 852 Size: 240 32 17 32 415 32 84 Fragments arranged by size: 415 240 84 32 32 32 17 HincII GTy'rAC Cuts at: 0 624 852 Size: 624 228 HpaI GTT'AAC Cuts at: 0 624 852 Size: 624 228 KasI G'GCGC_C Cuts at: 0 424 852 Size: 424 428 MslI CAynn'nnrTG Cuts at: 0 52 852 Size: 52 800 MspA1I CmG'CkG Cuts at: 0 79 852 Size: 79 773 NarI GG'CG_CC Cuts at: 0 425 852 Size: 425 427 NdeI CA'TA_TG Cuts at: 0 173 852 Size: 173 679 NsiI A_TGCA'T Cuts at: 0 474 852 Size: 474 378 NspI r_CATG'y Cuts at: 0 39 852 Size: 39 813 PasI CC'CwG_GG Cuts at: 0 61 763 852 Size: 61 702 89 Fragments arranged by size: 702 89 61 PspOMI G'GGCC_C Cuts at: 0 348 852 Size: 348 504 PvuII CAG'CTG Cuts at: 0 79 852 Size: 79 773 SfcI C'TryA_G Cuts at: 0 225 336 852 Size: 225 111 516 Fragments arranged by size: 516 225 111 SfoI GGC'GCC Cuts at: 0 426 852 Size: 426 426 SmaI CCC'GGG Cuts at: 0 44 852 Size: 44 808 StyI C'CwwG_G Cuts at: 0 431 677 852 Size: 431 246 175 Fragments arranged by size: 431 246 175 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 346 852 Size: 346 506 TatI w'GTAC_w Cuts at: 0 271 852 Size: 271 581 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 719 751 852 Size: 719 32 101 Fragments arranged by size: 719 101 32 XmaI C'CCGG_G Cuts at: 0 42 852 Size: 42 810 Enzymes that do cut: AarI AlfI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglI Bme1580I BmgBI BmrI BpmI BsaHI BsaXI BseYI BsgI BsiEI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BstYI EaeI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HpaI KasI MslI MspA1I NarI NdeI NsiI NspI PasI PspOMI PvuII SfcI SfoI SmaI StyI TaqII TatI TstI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BglII BlpI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsiHKAI BsiWI BsmI BspEI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRV FalI FseI FspAI HindIII KpnI MfeI MluI MmeI MscI NaeI NcoI NgoMIV NheI NotI NruI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI