Restriction map for
pFN21AE9513
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# NO CUT ENZYMES ( 139 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AflII AgeI AhdI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII BanI BanII BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
CfoI ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI HinP1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SphI Sse8387I SspBI SspI
SwaI TfiI Tsp509I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 941 GACGTC GACGT^C
AcyI 941 GACGTC G[GA]^CG[CT]C
AflIII 218 ACATGT A^C[GA][CT]GT
AocI 1021 CCTGAGG CC^T[ACGT]AGG
BamHI 903 GGATCC G^GATCC
BbsI 711 GTCTTC GAAGAC(2/6)
BbvCI 39 CCTCAGC CCTCAGC(-5/-2)
BglI 449 GCCTCGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 317 AGATCT A^GATCT
BpuAI 711 GTCTTC GAAGAC(2/6)
BsaHI 941 GACGTC G[GA]^CG[CT]C
BsgI 701 CTGCAC GTGCAG(16/14)
BspLU11I 218 ACATGT A^CATGT
BsrBI 723 CCGCTC CCGCTC(-3/-3)
BsrFI 490 GCCGGC [GA]^CCGG[CT]
BssSI 499 CTCGTG CACGAG(-5/-1)
Bsu36I 1021 CCTGAGG CC^T[ACGT]AGG
Cfr10I 490 GCCGGC [GA]^CCGG[CT]
DraII 48 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 586 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1021 CCTGAGG CC^T[ACGT]AGG
EcoNI 1017 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 48 AGGTCCT [GA]G^G[ACGT]CC[CT]
HapII 491 CCGG C^CGG
Hin1I 941 GACGTC G[GA]^CG[CT]C
HincII 850 GTCAAC GT[CT]^[GA]AC
HindII 850 GTCAAC GT[CT]^[GA]AC
HpaII 491 CCGG C^CGG
MseI 155 TTAA T^TAA
MspI 491 CCGG C^CGG
NaeI 490 GCCGGC GCC^GGC
NgoMIV 490 GCCGGC G^CCGGC
NspI 218 ACATGT [GA]CATG^[CT]
PflMI 46 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 48 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 540 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 174 GCTCTTC GCTCTTC(1/4)
ScaI 837 AGTACT AGT^ACT
SpeI 1008 ACTAGT A^CTAGT
TaqI 939 TCGA T^CGA
Tru9I 155 TTAA T^TAA
TthHB8I 939 TCGA T^CGA
Van91I 46 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 8 GTAC GT^AC
838 GTAC
Alw44I 391 GTGCAC G^TGCAC
592 GTGCAC
ApaLI 391 GTGCAC G^TGCAC
592 GTGCAC
AvaII 49 GGTCC G^G[AT]CC
539 GGACC
AvrII 33 CCTAGG C^CTAGG
1078 CCTAGG
BlnI 33 CCTAGG C^CTAGG
1078 CCTAGG
BspMI 379 ACCTGC ACCTGC(4/8)
1037 GCAGGT
BstF5I 105 CATCC GGATG(2/0)
201 GGATG
EcoT14I 33 CCTAGG C^C[AT][AT]GG
1078 CCTAGG
FauI 791 CCCGC CCCGC(4/6)
983 CCCGC
FokI 105 CATCC GGATG(9/13)
201 GGATG
HphI 377 TCACC GGTGA(8/7)
788 TCACC
MaeII 854 ACGT A^CGT
942 ACGT
MspA1I 894 CAGCTG C[AC]G^C[GT]G
1032 CAGCTG
PstI 360 CTGCAG CTGCA^G
1035 CTGCAG
PvuII 894 CAGCTG CAG^CTG
1032 CAGCTG
RsaI 8 GTAC GT^AC
838 GTAC
SfcI 360 CTGCAG C^T[GA][CT]AG
1035 CTGCAG
StuI 36 AGGCCT AGG^CCT
456 AGGCCT
StyI 33 CCTAGG C^C[AT][AT]GG
1078 CCTAGG
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
BalI BbvI BfaI BmyI BsaJI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII Fnu4HI HaeIII HinfI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI TseI Tsp45I TspRI XcmI
XhoII