(Linear) (Six-base) MAPSORT of: KIEE9513.seq Check: 8387 from: 1 to: 1095 KIEE9513.orf With 182 enzymes: SgfI * December 27, 2011 16:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 388 1028 1095 Size: 388 640 67 Fragments arranged by size: 640 388 67 AatII G_ACGT'C Cuts at: 0 945 1095 Size: 945 150 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 582 593 1095 Size: 582 11 502 Fragments arranged by size: 582 502 11 AflIII A'CryG_T Cuts at: 0 218 1095 Size: 218 877 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 406 1095 Size: 406 689 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 85 117 1095 Size: 85 32 978 Fragments arranged by size: 978 85 32 AlwNI CAG_nnn'CTG Cuts at: 0 52 700 1067 1095 Size: 52 648 367 28 Fragments arranged by size: 648 367 52 28 ApaLI G'TGCA_C Cuts at: 0 391 592 1095 Size: 391 201 503 Fragments arranged by size: 503 391 201 AvrII C'CTAG_G Cuts at: 0 33 1078 1095 Size: 33 1045 17 Fragments arranged by size: 1045 33 17 BamHI G'GATC_C Cuts at: 0 903 1095 Size: 903 192 BbsI GAAGACnn'nnnn_ Cuts at: 0 704 1095 Size: 704 391 BbvCI CC'TCA_GC Cuts at: 0 40 1095 Size: 40 1055 BglI GCCn_nnn'nGGC Cuts at: 0 455 1095 Size: 455 640 BglII A'GATC_T Cuts at: 0 317 1095 Size: 317 778 Bme1580I G_kGCm'C Cuts at: 0 395 429 506 596 635 1095 Size: 395 34 77 90 39 460 Fragments arranged by size: 460 395 90 77 39 34 BmrI ACTGGGnnnn_n' Cuts at: 0 935 1095 Size: 935 160 Bpu10I CC'TnA_GC Cuts at: 0 40 1095 Size: 40 1055 BsaHI Gr'CG_yC Cuts at: 0 942 1095 Size: 942 153 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 932 962 1095 Size: 932 30 133 Fragments arranged by size: 932 133 30 BseYI C'CCAG_C Cuts at: 0 141 434 977 1095 Size: 141 293 543 118 Fragments arranged by size: 543 293 141 118 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 686 1095 Size: 686 409 BsiHKAI G_wGCw'C Cuts at: 0 395 596 914 1095 Size: 395 201 318 181 Fragments arranged by size: 395 318 201 181 Bsp1286I G_dGCh'C Cuts at: 0 395 429 506 596 635 914 1095 Size: 395 34 77 90 39 279 181 Fragments arranged by size: 395 279 181 90 77 39 34 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 388 1028 1095 Size: 388 640 67 Fragments arranged by size: 640 388 67 BsrBI CCG'CTC Cuts at: 0 725 1095 Size: 725 370 BsrFI r'CCGG_y Cuts at: 0 490 1095 Size: 490 605 BssSI C'ACGA_G Cuts at: 0 499 1095 Size: 499 596 BstXI CCAn_nnnn'nTGG Cuts at: 0 170 744 819 1095 Size: 170 574 75 276 Fragments arranged by size: 574 276 170 75 BstYI r'GATC_y Cuts at: 0 28 317 903 1095 Size: 28 289 586 192 Fragments arranged by size: 586 289 192 28 Bsu36I CC'TnA_GG Cuts at: 0 1022 1095 Size: 1022 73 BtsI GCAGTG_nn' Cuts at: 0 66 369 1095 Size: 66 303 726 Fragments arranged by size: 726 303 66 DraIII CAC_nnn'GTG Cuts at: 0 591 1095 Size: 591 504 EaeI y'GGCC_r Cuts at: 0 2 209 809 881 1046 1095 Size: 2 207 600 72 165 49 Fragments arranged by size: 600 207 165 72 49 2 EarI CTCTTCn'nnn_ Cuts at: 0 181 569 1002 1095 Size: 181 388 433 93 Fragments arranged by size: 433 388 181 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 175 1095 Size: 175 920 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 582 593 1095 Size: 582 11 502 Fragments arranged by size: 582 502 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 1021 1095 Size: 1021 74 EcoO109I rG'GnC_Cy Cuts at: 0 49 1095 Size: 49 1046 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 932 964 1095 Size: 932 32 131 Fragments arranged by size: 932 131 32 HincII GTy'rAC Cuts at: 0 852 1095 Size: 852 243 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 216 800 1095 Size: 216 584 295 Fragments arranged by size: 584 295 216 MscI TGG'CCA Cuts at: 0 4 811 883 1095 Size: 4 807 72 212 Fragments arranged by size: 807 212 72 4 MslI CAynn'nnrTG Cuts at: 0 217 388 742 1095 Size: 217 171 354 353 Fragments arranged by size: 354 353 217 171 MspA1I CmG'CkG Cuts at: 0 896 1034 1095 Size: 896 138 61 Fragments arranged by size: 896 138 61 NaeI GCC'GGC Cuts at: 0 492 1095 Size: 492 603 NgoMIV G'CCGG_C Cuts at: 0 490 1095 Size: 490 605 NspI r_CATG'y Cuts at: 0 222 1095 Size: 222 873 PasI CC'CwG_GG Cuts at: 0 197 1095 Size: 197 898 PciI A'CATG_T Cuts at: 0 218 1095 Size: 218 877 PflMI CCAn_nnn'nTGG Cuts at: 0 52 1095 Size: 52 1043 PpuMI rG'GwC_Cy Cuts at: 0 49 1095 Size: 49 1046 PshAI GACnn'nnGTC Cuts at: 0 544 1095 Size: 544 551 PstI C_TGCA'G Cuts at: 0 364 1039 1095 Size: 364 675 56 Fragments arranged by size: 675 364 56 PvuII CAG'CTG Cuts at: 0 896 1034 1095 Size: 896 138 61 Fragments arranged by size: 896 138 61 SapI GCTCTTCn'nnn_ Cuts at: 0 181 1095 Size: 181 914 ScaI AGT'ACT Cuts at: 0 839 1095 Size: 839 256 SfcI C'TryA_G Cuts at: 0 360 1035 1095 Size: 360 675 60 Fragments arranged by size: 675 360 60 SpeI A'CTAG_T Cuts at: 0 1008 1095 Size: 1008 87 StuI AGG'CCT Cuts at: 0 38 458 1095 Size: 38 420 637 Fragments arranged by size: 637 420 38 StyI C'CwwG_G Cuts at: 0 33 1078 1095 Size: 33 1045 17 Fragments arranged by size: 1045 33 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 431 1095 Size: 431 664 TatI w'GTAC_w Cuts at: 0 7 837 1095 Size: 7 830 258 Fragments arranged by size: 830 258 7 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 304 804 897 1095 Size: 304 500 93 198 Fragments arranged by size: 500 304 198 93 ZraI GAC'GTC Cuts at: 0 943 1095 Size: 943 152 Enzymes that do cut: AarI AatII AcuI AflIII AlfI AloI AlwNI ApaLI AvrII BamHI BbsI BbvCI BglI BglII Bme1580I BmrI Bpu10I BsaHI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstXI BstYI Bsu36I BtsI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I Hin4I HincII MmeI MscI MslI MspA1I NaeI NgoMIV NspI PasI PciI PflMI PpuMI PshAI PstI PvuII SapI ScaI SfcI SpeI StuI StyI TaqII TatI XcmI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AgeI AhdI AleI ApaI ApoI AscI AseI AsiSI AvaI BaeI BanI BanII BbeI BcgI BciVI BclI BfrBI BlpI BmgBI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI XmnI