Restriction map for
pFN21AE5370
##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbrPI BcgI BciVI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsgI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tsp509I Tth111I
XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccIII 77 TCCGGA T^CCGGA
AcyI 832 GGCGCC G[GA]^CG[CT]C
ApaI 668 GGGCCC GGGCC^C
AvaII 96 GGTCC G^G[AT]CC
AviII 632 TGCGCA TGC^GCA
BalI 236 TGGCCA TGG^CCA
BanI 832 GGCGCC G^G[CT][GA]CC
BanII 668 GGGCCC G[GA]GC[CT]^C
BbeI 832 GGCGCC GGCGC^C
BbvCI 663 GCTGAGG CCTCAGC(-5/-2)
BclI 728 TGATCA T^GATCA
BglI 157 GCCGAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 832 GGCGCC G[GA]^CG[CT]C
BsaI 577 GAGACC GGTCTC(1/5)
BsaWI 77 TCCGGA [AT]^CCGG[AT]
BseAI 77 TCCGGA T^CCGGA
BsiEI 440 CGGCCG CG[GA][CT]^CG
BsmBI 856 CGTCTC CGTCTC(1/5)
BsmFI 97 GTCCC GGGAC(10/14)
BspEI 77 TCCGGA T^CCGGA
BspHI 608 TCATGA T^CATGA
BspMI 773 ACCTGC ACCTGC(4/8)
BstAPI 57 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 413 CCAGGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 74 AGATCC [GA]^GATC[CT]
DsaI 638 CCACGG C^C[GA][CT]GG
EagI 440 CGGCCG C^GGCCG
EarI 12 GAAGAG CTCTTC(1/4)
EclXI 440 CGGCCG C^GGCCG
Eco52I 440 CGGCCG C^GGCCG
FbaI 728 TGATCA T^GATCA
FspI 632 TGCGCA TGC^GCA
HgaI 855 GCGTC GACGC(5/10)
Hin1I 832 GGCGCC G[GA]^CG[CT]C
HincII 544 GTCGAC GT[CT]^[GA]AC
HindII 544 GTCGAC GT[CT]^[GA]AC
HphI 702 GGTGA GGTGA(8/7)
KasI 832 GGCGCC G^GCGCC
Ksp632I 12 GAAGAG CTCTTC(1/4)
MaeIII 814 GTGAC ^GT[ACGT]AC
MflI 74 AGATCC [GA]^GATC[CT]
MluNI 236 TGGCCA TGG^CCA
MroI 77 TCCGGA T^CCGGA
MscI 236 TGGCCA TGG^CCA
MseI 85 TTAA T^TAA
MslI 696 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 832 GGCGCC GG^CGCC
NspI 455 GCATGT [GA]CATG^[CT]
PaeR7I 823 CTCGAG C^TCGAG
PstI 64 CTGCAG CTGCA^G
RcaI 608 TCATGA T^CATGA
SalI 544 GTCGAC G^TCGAC
SfoI 832 GGCGCC GGC^GCC
SmlI 823 CTCGAG C^T[CT][GA]AG
Tru9I 85 TTAA T^TAA
Tsp45I 814 GTGAC ^GT[GC]AC
TspRI 451 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 239 CCAAGATGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 823 CTCGAG C^TCGAG
XhoII 74 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 544 GTCGAC GT^[AC][GT]AC
769 GTCTAC
AfaI 459 GTAC GT^AC
744 GTAC
AlwNI 58 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
181 CAGGAGCTG
AvaI 574 CCCGAG C^[CT]CG[GA]G
823 CTCGAG
BbsI 229 GAAGAC GAAGAC(2/6)
687 GAAGAC
BcnI 438 CCCGG CC^[GC]GG
839 CCGGG
BpuAI 229 GAAGAC GAAGAC(2/6)
687 GAAGAC
BsmAI 577 GAGAC GTCTC(1/5)
857 GTCTC
BsoBI 574 CCCGAG C^[CT]CG[GA]G
823 CTCGAG
BsrBI 267 CCGCTC CCGCTC(-3/-3)
820 CCGCTC
DraII 667 AGGGCCC [GA]G^G[ACGT]CC[CT]
668 GGGCCCT
EcoO109I 667 AGGGCCC [GA]G^G[ACGT]CC[CT]
668 GGGCCCT
HaeII 320 AGCGCC [GA]GCGC^[CT]
832 GGCGCC
MspA1I 478 CAGCTG C[AC]G^C[GT]G
655 CAGCTG
NaeI 364 GCCGGC GCC^GGC
829 GCCGGC
NciI 438 CCCGG CC^[GC]GG
839 CCGGG
NgoMIV 364 GCCGGC G^CCGGC
829 GCCGGC
PflMI 135 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
638 CCACGGCATGG
PvuII 478 CAGCTG CAG^CTG
655 CAGCTG
RsaI 459 GTAC GT^AC
744 GTAC
SfaNI 504 GATGC GCATC(5/9)
332 GCATC
SfcI 64 CTGCAG C^T[GA][CT]AG
786 CTACAG
TfiI 217 GAATC G^A[AT]TC
246 GAATC
Van91I 135 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
638 CCACGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
BbvI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI Bsp1286I BsrFI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeII MboI MboII
MnlI MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI TaqI TseI TthHB8I