Restriction map for
pFN21AE5370
(Circular) (Six-base) MAPSORT of: pFN21AE5370.seq Check: 240 from: 1 to: 5569
pFN21AE5370 5569 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3379 278
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2550 2775 2912 1937
Size: 613 225 137 4594
Fragments arranged by size:
4594 613 225 137
Acc65I G'GTAC_C
Cuts at: 2890 2890
Size: 5569
AclI AA'CG_TT
Cuts at: 1563 3696 4069 1563
Size: 2133 373 3063
Fragments arranged by size:
3063 2133 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2554 2711 3632 5016 5284 1632
Size: 922 157 921 1384 268 1917
Fragments arranged by size:
1917 1384 922 921 268 157
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5372
AflIII A'CryG_T
Cuts at: 1203 4468 5445 1203
Size: 3265 977 1327
Fragments arranged by size:
3265 1327 977
AgeI A'CCGG_T
Cuts at: 4400 4400
Size: 5569
AhdI GACnn_n'nnGTC
Cuts at: 4298 4298
Size: 5569
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5569
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5569
AlwNI CAG_nnn'CTG
Cuts at: 2068 2191 3312 4884 2068
Size: 123 1121 1572 2753
Fragments arranged by size:
2753 1572 1121 123
ApaI G_GGCC'C
Cuts at: 2677 2677
Size: 5569
ApaLI G'TGCA_C
Cuts at: 3626 4782 3626
Size: 1156 4413
ApoI r'AATT_y
Cuts at: 1493 2878 3104 3256 5365 1493
Size: 1385 226 152 2109 1697
Fragments arranged by size:
2109 1697 1385 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4123 160
Size: 872 3091 1606
Fragments arranged by size:
3091 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
AvaI C'yCGr_G
Cuts at: 1870 1943 2579 2828 2894 1870
Size: 73 636 249 66 4545
Fragments arranged by size:
4545 636 249 73 66
BamHI G'GATC_C
Cuts at: 2899 3266 2899
Size: 367 5202
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2837 2890 4345 618
Size: 325 217 627 1050 53 1455 1842
Fragments arranged by size:
1842 1455 1050 627 325 217 53
BanII G_rGCy'C
Cuts at: 1391 2677 2888 1391
Size: 1286 211 4072
Fragments arranged by size:
4072 1286 211
BbeI G_GCGC'C
Cuts at: 2841 2841
Size: 5569
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2241 2699 928
Size: 971 342 458 3798
Fragments arranged by size:
3798 971 458 342
BbvCI CC'TCA_GC
Cuts at: 2669 2669
Size: 5569
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3760 3794 1903
Size: 34 1823 34 3678
Fragments arranged by size:
3678 1823 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3461 4671 3461
Size: 1210 4359
BclI T'GATC_A
Cuts at: 1548 2733 1548
Size: 1185 4384
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2168 4180 136
Size: 107 122 71 631 605 496 2012 1525
Fragments arranged by size:
2012 1525 631 605 496 122 107 71
BglII A'GATC_T
Cuts at: 5564 5564
Size: 5569
BlpI GC'TnA_GC
Cuts at: 2986 2986
Size: 5569
Bme1580I G_kGCm'C
Cuts at: 1790 2677 3630 4786 1790
Size: 887 953 1156 2573
Fragments arranged by size:
2573 1156 953 887
BmgBI CAC'GTC
Cuts at: 1318 2146 2548 2686 1318
Size: 828 402 138 4201
Fragments arranged by size:
4201 828 402 138
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3300 4258 442
Size: 416 53 83 399 24 1883 958 1753
Fragments arranged by size:
1883 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5569
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 2447 2684 4229 1239
Size: 974 234 237 1545 2579
Fragments arranged by size:
2579 1545 974 237 234
Bpu10I CC'TnA_GC
Cuts at: 2669 2669
Size: 5569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3694 4559 4857 3694
Size: 865 298 4406
Fragments arranged by size:
4406 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2576 4232 882
Size: 617 1077 1656 2219
Fragments arranged by size:
2219 1656 1077 617
BsaAI yAC'GTr
Cuts at: 493 1204 3360 493
Size: 711 2156 2702
Fragments arranged by size:
2702 2156 711
BsaBI GATnn'nnATC
Cuts at: 5563 5563
Size: 5569
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2838 3376 3758 4393
Size: 53 83 186 936 1305 538 382 635
Cuts at: 4393 275
Size: 1451
Fragments arranged by size:
1451 1305 936 635 538 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2082 4002 4400 4674 4821 5232 2082
Size: 1920 398 274 147 411 2419
Fragments arranged by size:
2419 1920 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2798 2828 1178
Size: 30 165 30 1395 30 3919
Fragments arranged by size:
3919 1395 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2266 2330 2399 2402 2405 2759 1186
Size: 1080 64 69 3 3 354 3996
Fragments arranged by size:
3996 1080 354 69 64 3 3
BseYI C'CCAG_C
Cuts at: 4772 5268 4772
Size: 496 5073
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4996
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2448 3019 3780 4808 1540
Size: 275 186 447 571 761 1028 2301
Fragments arranged by size:
2301 1028 761 571 447 275 186
BsiHKAI G_wGCw'C
Cuts at: 2364 2808 2856 3630 3715 4786 2364
Size: 444 48 774 85 1071 3147
Fragments arranged by size:
3147 1071 774 444 85 48
BsmI GAATG_Cn'
Cuts at: 3080 3173 3080
Size: 93 5476
BsmBI CGTCTCn'nnnn_
Cuts at: 2867 2867
Size: 5569
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2364 2677 2808 2856 2888 3630 3715
Size: 399 574 313 131 48 32 742 85
Cuts at: 3715 4786 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 574 399 313 131 85
48 32
BspEI T'CCGG_A
Cuts at: 2082 5232 2082
Size: 3150 2419
BspHI T'CATG_A
Cuts at: 2613 3458 5188 2613
Size: 845 1730 2994
Fragments arranged by size:
2994 1730 845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2787 2924 844
Size: 1943 137 3489
Fragments arranged by size:
3489 1943 137
BsrBI CCG'CTC
Cuts at: 1394 1823 2274 2827 3456 1394
Size: 429 451 553 629 3507
Fragments arranged by size:
3507 629 553 451 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4064 4238 65
Size: 1183 2816 174 1396
Fragments arranged by size:
2816 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2181 2262 2369 2834 4213 4400 1700
Size: 110 371 81 107 465 1379 187 2869
Fragments arranged by size:
2869 1379 465 371 187 110 107 81
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5569
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5569
BssSI C'ACGA_G
Cuts at: 3629 4641 3629
Size: 1012 4557
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2068 794
Size: 1274 4295
BstBI TT'CG_AA
Cuts at: 4373 4373
Size: 5569
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5569
BstXI CCAn_nnnn'nTGG
Cuts at: 2425 2425
Size: 5569
BstYI r'GATC_y
Cuts at: 1972 2079 2899 3266 3651 3668 5120 5206 5218
Size: 107 820 367 385 17 1452 86 12
Cuts at: 5218 5564 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 820 385 367 346 107 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2643 513
Size: 552 939 639 3439
Fragments arranged by size:
3439 939 639 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4830
Fragments arranged by size:
4830 683 56
BtsI GCAGTG_nn'
Cuts at: 2455 3085 3879 3899 2455
Size: 630 794 20 4125
Fragments arranged by size:
4125 794 630 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5569
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5534
DraI TTT'AAA
Cuts at: 2873 2873
Size: 5569
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4576 809
Size: 507 3260 1802
Fragments arranged by size:
3260 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 2367 2445
Size: 54 1425 50 275 18 411 126 78
Cuts at: 2445 2452 3016 3905 5534 8
Size: 7 564 889 1629 43
Fragments arranged by size:
1629 1425 889 564 411 275 126 78
54 50 43 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 2445 3016 1537
Size: 275 633 571 4090
Fragments arranged by size:
4090 633 571 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2012 3499 1353
Size: 659 1487 3423
Fragments arranged by size:
3423 1487 659
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4156 4530 4676 387
Size: 1064 138 2567 374 146 1280
Fragments arranged by size:
2567 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2447 2554 2684 2711 3632 4229
Size: 393 581 234 107 130 27 921 597
Cuts at: 4229 5016 5284 1239
Size: 787 268 1524
Fragments arranged by size:
1524 921 787 597 581 393 268 234
130 107 27
EcoO109I rG'GnC_Cy
Cuts at: 2673 2674 2673
Size: 1 5568
EcoRI G'AATT_C
Cuts at: 2878 3256 2878
Size: 378 5191
FspI TGC'GCA
Cuts at: 2639 4075 2639
Size: 1436 4133
FspAI rTGC'GCAy
Cuts at: 2639 2639
Size: 5569
HaeII r_GCGC'y
Cuts at: 1678 2329 2841 4716 1678
Size: 651 512 1875 2531
Fragments arranged by size:
2531 1875 651 512
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4210 4242 4284 4316 1373
Size: 32 135 32 2638 32 42 32 2626
Fragments arranged by size:
2638 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2551 2913 3159 669
Size: 988 281 613 362 246 3079
Fragments arranged by size:
3079 988 613 362 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5569
HpaI GTT'AAC
Cuts at: 3159 3159
Size: 5569
KasI G'GCGC_C
Cuts at: 2837 2837
Size: 5569
KpnI G_GTAC'C
Cuts at: 2894 2894
Size: 5569
MfeI C'AATT_G
Cuts at: 3168 3168
Size: 5569
MluI A'CGCG_T
Cuts at: 5445 5445
Size: 5569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2337 4683 4867 1305
Size: 1032 2346 184 2007
Fragments arranged by size:
2346 2007 1032 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2243 10
Size: 54 1425 343 411 3336
Fragments arranged by size:
3336 1425 411 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2705 3527 3886 4045 518
Size: 648 1539 822 359 159 2042
Fragments arranged by size:
2042 1539 822 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2485 2662 2985 3662 4810 5055 1606
Size: 879 177 323 677 1148 245 2120
Fragments arranged by size:
2120 1148 879 677 323 245 177
NaeI GCC'GGC
Cuts at: 2371 2836 2371
Size: 465 5104
NarI GG'CG_CC
Cuts at: 2838 2838
Size: 5569
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4078
Fragments arranged by size:
4078 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5569
NgoMIV G'CCGG_C
Cuts at: 2369 2834 2369
Size: 465 5104
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5569
NotI GC'GGCC_GC
Cuts at: 3016 3016
Size: 5569
NspI r_CATG'y
Cuts at: 1132 1229 2464 2927 4472 1132
Size: 97 1235 463 1545 2229
Fragments arranged by size:
2229 1545 1235 463 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5569
PciI A'CATG_T
Cuts at: 4468 4468
Size: 5569
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2146 2649 1108
Size: 1038 503 4028
Fragments arranged by size:
4028 1038 503
PmeI GTTT'AAAC
Cuts at: 2873 2873
Size: 5569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 5151 5183 3638
Size: 32 1481 32 4024
Fragments arranged by size:
4024 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5569
PsiI TTA'TAA
Cuts at: 3139 3139
Size: 5569
PspOMI G'GGCC_C
Cuts at: 2673 2673
Size: 5569
PspXI vC'TCGA_Gb
Cuts at: 2828 2828
Size: 5569
PstI C_TGCA'G
Cuts at: 830 2073 2921 830
Size: 1243 848 3478
Fragments arranged by size:
3478 1243 848
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5569
PvuII CAG'CTG
Cuts at: 2485 2662 2485
Size: 177 5392
SalI G'TCGA_C
Cuts at: 1936 2549 2911 1936
Size: 613 362 4594
Fragments arranged by size:
4594 613 362
SbfI CC_TGCA'GG
Cuts at: 2921 2921
Size: 5569
ScaI AGT'ACT
Cuts at: 3817 5529 3817
Size: 1712 3857
SfcI C'TryA_G
Cuts at: 826 1045 2069 2791 2917 4052 4733 4924 826
Size: 219 1024 722 126 1135 681 191 1471
Fragments arranged by size:
1471 1135 1024 722 681 219 191 126
SfoI GGC'GCC
Cuts at: 2839 2839
Size: 5569
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5569
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5569
SmaI CCC'GGG
Cuts at: 1872 2896 1872
Size: 1024 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2828 3673 4574 4836 820
Size: 197 926 885 845 901 262 1553
Fragments arranged by size:
1553 926 901 885 845 262 197
SnaBI TAC'GTA
Cuts at: 493 3360 493
Size: 2867 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5569
SphI G_CATG'C
Cuts at: 1132 2927 1132
Size: 1795 3774
SspI AAT'ATT
Cuts at: 5 52 3493 5
Size: 47 3441 2081
Fragments arranged by size:
3441 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5569
StyI C'CwwG_G
Cuts at: 513 1065 2004 3008 513
Size: 552 939 1004 3074
Fragments arranged by size:
3074 1004 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2089 2126 3596 3613 3766 3951 4396
Size: 1255 509 37 1470 17 153 185 445
Cuts at: 4396 325
Size: 1498
Fragments arranged by size:
1498 1470 1255 509 445 185 153 37
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3815 5527 96
Size: 274 80 33 51 1443 1838 1712 138
Fragments arranged by size:
1838 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2798 2830 5529 5561 2798
Size: 32 2699 32 2806
Fragments arranged by size:
2806 2699 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5569
XbaI T'CTAG_A
Cuts at: 2905 2905
Size: 5569
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2251 2251
Size: 5569
XhoI C'TCGA_G
Cuts at: 1943 2828 1943
Size: 885 4684
XmaI C'CCGG_G
Cuts at: 1870 2894 1870
Size: 1024 4545
XmnI GAAnn'nnTTC
Cuts at: 3698 3698
Size: 5569
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3377 276
Size: 53 83 186 936 1843 2468
Fragments arranged by size:
2468 1843 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI NruI NsiI PacI PfoI PmlI PpuMI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI