Restriction map for
pFN21AE5370
(Linear) (Six-base) MAPSORT of: KIEE5370.seq Check: 9088 from: 1 to: 864
KIEE5370.orf
With 182 enzymes: SgfI *
December 5, 2014 13:01 ..
AccI GT'mk_AC
Cuts at: 0 545 770 864
Size: 545 225 94
Fragments arranged by size:
545 225 94
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 706 864
Size: 549 157 158
Fragments arranged by size:
549 158 157
AlwNI CAG_nnn'CTG
Cuts at: 0 63 186 864
Size: 63 123 678
Fragments arranged by size:
678 123 63
ApaI G_GGCC'C
Cuts at: 0 672 864
Size: 672 192
AvaI C'yCGr_G
Cuts at: 0 574 823 864
Size: 574 249 41
Fragments arranged by size:
574 249 41
BanI G'GyrC_C
Cuts at: 0 832 864
Size: 832 32
BanII G_rGCy'C
Cuts at: 0 672 864
Size: 672 192
BbeI G_GCGC'C
Cuts at: 0 836 864
Size: 836 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 236 694 864
Size: 236 458 170
Fragments arranged by size:
458 236 170
BbvCI CC'TCA_GC
Cuts at: 0 664 864
Size: 664 200
BclI T'GATC_A
Cuts at: 0 728 864
Size: 728 136
BglI GCCn_nnn'nGGC
Cuts at: 0 163 864
Size: 163 701
Bme1580I G_kGCm'C
Cuts at: 0 672 864
Size: 672 192
BmgBI CAC'GTC
Cuts at: 0 141 543 681 864
Size: 141 402 138 183
Fragments arranged by size:
402 183 141 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 442 679 864
Size: 208 234 237 185
Fragments arranged by size:
237 234 208 185
Bpu10I CC'TnA_GC
Cuts at: 0 664 864
Size: 664 200
BsaI GGTCTCn'nnnn_
Cuts at: 0 571 864
Size: 571 293
BsaHI Gr'CG_yC
Cuts at: 0 833 864
Size: 833 31
BsaWI w'CCGG_w
Cuts at: 0 77 864
Size: 77 787
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 793 823 864
Size: 793 30 41
Fragments arranged by size:
793 41 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 261 325 394 397 400 754 864
Size: 261 64 69 3 3 354 110
Fragments arranged by size:
354 261 110 69 64 3 3
BsiEI CG_ry'CG
Cuts at: 0 443 864
Size: 443 421
BsiHKAI G_wGCw'C
Cuts at: 0 359 803 851 864
Size: 359 444 48 13
Fragments arranged by size:
444 359 48 13
BsmBI CGTCTCn'nnnn_
Cuts at: 0 862 864
Size: 862 2
Bsp1286I G_dGCh'C
Cuts at: 0 359 672 803 851 864
Size: 359 313 131 48 13
Fragments arranged by size:
359 313 131 48 13
BspEI T'CCGG_A
Cuts at: 0 77 864
Size: 77 787
BspHI T'CATG_A
Cuts at: 0 608 864
Size: 608 256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 782 864
Size: 782 82
BsrBI CCG'CTC
Cuts at: 0 269 822 864
Size: 269 553 42
Fragments arranged by size:
553 269 42
BsrFI r'CCGG_y
Cuts at: 0 176 257 364 829 864
Size: 176 81 107 465 35
Fragments arranged by size:
465 176 107 81 35
BstAPI GCAn_nnn'nTGC
Cuts at: 0 63 864
Size: 63 801
BstXI CCAn_nnnn'nTGG
Cuts at: 0 420 864
Size: 420 444
BstYI r'GATC_y
Cuts at: 0 74 864
Size: 74 790
BtgI C'CryG_G
Cuts at: 0 638 864
Size: 638 226
BtsI GCAGTG_nn'
Cuts at: 0 450 864
Size: 450 414
EaeI y'GGCC_r
Cuts at: 0 236 362 440 447 864
Size: 236 126 78 7 417
Fragments arranged by size:
417 236 126 78 7
EagI C'GGCC_G
Cuts at: 0 440 864
Size: 440 424
EarI CTCTTCn'nnn_
Cuts at: 0 7 864
Size: 7 857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 442 549 679 706 864
Size: 208 234 107 130 27 158
Fragments arranged by size:
234 208 158 130 107 27
EcoO109I rG'GnC_Cy
Cuts at: 0 668 669 864
Size: 668 1 195
Fragments arranged by size:
668 195 1
FspI TGC'GCA
Cuts at: 0 634 864
Size: 634 230
FspAI rTGC'GCAy
Cuts at: 0 634 864
Size: 634 230
HaeII r_GCGC'y
Cuts at: 0 324 836 864
Size: 324 512 28
Fragments arranged by size:
512 324 28
HincII GTy'rAC
Cuts at: 0 546 864
Size: 546 318
KasI G'GCGC_C
Cuts at: 0 832 864
Size: 832 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 864
Size: 332 532
MscI TGG'CCA
Cuts at: 0 238 864
Size: 238 626
MslI CAynn'nnrTG
Cuts at: 0 700 864
Size: 700 164
MspA1I CmG'CkG
Cuts at: 0 480 657 864
Size: 480 177 207
Fragments arranged by size:
480 207 177
NaeI GCC'GGC
Cuts at: 0 366 831 864
Size: 366 465 33
Fragments arranged by size:
465 366 33
NarI GG'CG_CC
Cuts at: 0 833 864
Size: 833 31
NgoMIV G'CCGG_C
Cuts at: 0 364 829 864
Size: 364 465 35
Fragments arranged by size:
465 364 35
NspI r_CATG'y
Cuts at: 0 459 864
Size: 459 405
PflMI CCAn_nnn'nTGG
Cuts at: 0 141 644 864
Size: 141 503 220
Fragments arranged by size:
503 220 141
PspOMI G'GGCC_C
Cuts at: 0 668 864
Size: 668 196
PspXI vC'TCGA_Gb
Cuts at: 0 823 864
Size: 823 41
PstI C_TGCA'G
Cuts at: 0 68 864
Size: 68 796
PvuII CAG'CTG
Cuts at: 0 480 657 864
Size: 480 177 207
Fragments arranged by size:
480 207 177
SalI G'TCGA_C
Cuts at: 0 544 864
Size: 544 320
SfcI C'TryA_G
Cuts at: 0 64 786 864
Size: 64 722 78
Fragments arranged by size:
722 78 64
SfoI GGC'GCC
Cuts at: 0 834 864
Size: 834 30
SmlI C'TyrA_G
Cuts at: 0 823 864
Size: 823 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 84 121 864
Size: 84 37 743
Fragments arranged by size:
743 84 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 793 825 864
Size: 793 32 39
Fragments arranged by size:
793 39 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 246 864
Size: 246 618
XhoI C'TCGA_G
Cuts at: 0 823 864
Size: 823 41
Enzymes that do cut:
AccI AcuI AlwNI ApaI AvaI BanI BanII BbeI
BbsI BbvCI BclI BglI Bme1580I BmgBI BpmI Bpu10I
BsaI BsaHI BsaWI BsaXI BseRI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BstAPI BstXI
BstYI BtgI BtsI EaeI EagI EarI Eco57MI EcoO109I
FspI FspAI HaeII HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PflMI PspOMI PspXI
PstI PvuII SalI SfcI SfoI SmlI TaqII TstI
XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BciVI BfrBI BglII
BlpI BmrI BmtI BplI BpuEI BsaAI BsaBI BseYI
BsgI BsiWI BsmI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
Hin4I HindIII HpaI KpnI MfeI MluI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PvuI
RsrII SacI SacII SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TatI Tth111I XbaI XmaI
XmnI ZraI