Restriction map for
pFN21AE3848
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
AvrII BamHI BbrPI BbvCI BclI
BglII BlnI BsaAI BsaBI BseAI
BsgI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BssHII Bst1107I
BstAPI BstEII BstPI BstZ17I ClaI
DraI DraIII DrdI Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NdeI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI SalI SapI SexAI
SfiI SnaBI SpeI SphI SspI
SwaI Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AflII 1401 CTTAAG C^TTAAG
AhdI 1969 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2149 CCTCAGG CC^T[ACGT]AGG
AspEI 1969 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 408 TGCGCA TGC^GCA
BcgI 1139 GCAACCACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1401 CTTAAG C^TTAAG
BlpI 1726 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1726 GCTCAGC GC^T[ACGT]AGC
BsiEI 75 CGGCCG CG[GA][CT]^CG
Bsp1407I 1031 TGTACA T^GTACA
BsrDI 1850 CATTGC GCAATG(2/0)
BsrGI 1031 TGTACA T^GTACA
BssSI 1473 CACGAG CACGAG(-5/-1)
BstBI 1147 TTCGAA TT^CGAA
BstXI 562 CCACACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2149 CCTCAGG CC^T[ACGT]AGG
CelII 1726 GCTCAGC GC^T[ACGT]AGC
CpoI 670 CGGTCCG CG^G[AT]CCG
EagI 75 CGGCCG C^GGCCG
Eam1105I 1969 GACCCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 75 CGGCCG C^GGCCG
Eco52I 75 CGGCCG C^GGCCG
Eco81I 2149 CCTCAGG CC^T[ACGT]AGG
FspI 408 TGCGCA TGC^GCA
MaeII 296 ACGT A^CGT
NaeI 102 GCCGGC GCC^GGC
NcoI 2340 CCATGG C^CATGG
NgoMIV 102 GCCGGC G^CCGGC
NheI 684 GCTAGC G^CTAGC
NspI 1388 GCATGT [GA]CATG^[CT]
NspV 1147 TTCGAA TT^CGAA
PvuII 847 CAGCTG CAG^CTG
RsrII 670 CGGTCCG CG^G[AT]CCG
SbfI 1602 CCTGCAGG CCTGCA^GG
ScaI 1837 AGTACT AGT^ACT
SfuI 1147 TTCGAA TT^CGAA
SgrAI 1332 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 1602 CCTGCAGG CCTGCA^GG
SspBI 1031 TGTACA T^GTACA
StuI 1159 AGGCCT AGG^CCT
XcmI 559 CCACCACACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AcsI 1313 AAATTC [GA]^AATT[CT]
1743 AAATTC
AlwI 1716 GGATC GGATC(4/5)
2107 GGATC
ApoI 1313 AAATTC [GA]^AATT[CT]
1743 AAATTC
Asp700I 1071 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1464 GAAATCTTTC
BbeI 717 GGCGCC GGCGC^C
1126 GGCGCC
BbsI 1191 GAAGAC GAAGAC(2/6)
1680 GAAGAC
BciVI 2013 GGATAC GTATCC(6/5)
59 GTATCC
BglI 162 GCCCGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
184 GCCAGCACGGC
BpmI 367 CTCCAG CTGGAG(16/14)
2136 CTCCAG
BpuAI 1191 GAAGAC GAAGAC(2/6)
1680 GAAGAC
BsaWI 313 TCCGGT [AT]^CCGG[AT]
1430 ACCGGA
BsrBI 584 CCGCTC CCGCTC(-3/-3)
381 GAGCGG
BsrFI 102 GCCGGC [GA]^CCGG[CT]
1333 ACCGGC
BstYI 1716 GGATCT [GA]^GATC[CT]
2107 GGATCT
Cfr10I 102 GCCGGC [GA]^CCGG[CT]
1333 ACCGGC
EarI 1657 CTCTTC CTCTTC(1/4)
1816 CTCTTC
EcoNI 1447 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1537 CCTGTGGCAGG
EcoT14I 350 CCAAGG C^C[AT][AT]GG
2340 CCATGG
KasI 717 GGCGCC G^GCGCC
1126 GGCGCC
Ksp632I 1657 CTCTTC CTCTTC(1/4)
1816 CTCTTC
MflI 1716 GGATCT [GA]^GATC[CT]
2107 GGATCT
NarI 717 GGCGCC GG^CGCC
1126 GGCGCC
PpuMI 113 AGGTCCC [GA]G^G[AT]CC[CT]
509 AGGACCT
SacI 1908 GAGCTC GAGCT^C
2058 GAGCTC
SfoI 717 GGCGCC GGC^GCC
1126 GGCGCC
StyI 350 CCAAGG C^C[AT][AT]GG
2340 CCATGG
TfiI 1880 GAATC G^A[AT]TC
2318 GATTC
XhoII 1716 GGATCT [GA]^GATC[CT]
2107 GGATCT
XmnI 1071 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1464 GAAATCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BmyI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KspI MaeI
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmaCI XmaI