(Circular) (Six-base) MAPSORT of: pFN21AE3848.seq Check: 7512 from: 1 to: 7102 pFN21AE3848 7102 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2874 2973 3333 3615 2874 Size: 99 360 282 6361 Fragments arranged by size: 6361 360 282 99 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4912 278 Size: 53 83 186 936 3376 2468 Fragments arranged by size: 3376 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4445 1937 Size: 2508 4594 Acc65I G'GTAC_C Cuts at: 4423 4423 Size: 7102 AclI AA'CG_TT Cuts at: 1563 5229 5602 1563 Size: 3666 373 3063 Fragments arranged by size: 3666 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2876 5165 6549 6817 1632 Size: 1244 2289 1384 268 1917 Fragments arranged by size: 2289 1917 1384 1244 268 AflII C'TTAA_G Cuts at: 820 1017 3406 820 Size: 197 2389 4516 Fragments arranged by size: 4516 2389 197 AflIII A'CryG_T Cuts at: 1203 6001 6978 1203 Size: 4798 977 1327 Fragments arranged by size: 4798 1327 977 AgeI A'CCGG_T Cuts at: 5933 5933 Size: 7102 AhdI GACnn_n'nnGTC Cuts at: 3979 5831 3979 Size: 1852 5250 AleI CACnn'nnGTG Cuts at: 1166 2655 3032 1166 Size: 1489 377 5236 Fragments arranged by size: 5236 1489 377 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3954 1184 Size: 2770 4332 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3065 3097 3065 Size: 32 7070 AlwNI CAG_nnn'CTG Cuts at: 2336 2518 3259 4845 6417 2336 Size: 182 741 1586 1572 3021 Fragments arranged by size: 3021 1586 1572 741 182 ApaI G_GGCC'C Cuts at: 2153 2169 2210 2366 2423 2765 2153 Size: 16 41 156 57 342 6490 Fragments arranged by size: 6490 342 156 57 41 16 ApaLI G'TGCA_C Cuts at: 5159 6315 5159 Size: 1156 5946 ApoI r'AATT_y Cuts at: 1493 3318 3748 4411 4637 4789 6898 1493 Size: 1825 430 663 226 152 2109 1697 Fragments arranged by size: 2109 1825 1697 663 430 226 152 AseI AT'TA_AT Cuts at: 160 1032 5656 160 Size: 872 4624 1606 Fragments arranged by size: 4624 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7102 AvaI C'yCGr_G Cuts at: 1870 1943 2145 2168 2365 2653 2813 3158 4427 Size: 73 202 23 197 288 160 345 1269 Cuts at: 4427 1870 Size: 4545 Fragments arranged by size: 4545 1269 345 288 202 197 160 73 23 BamHI G'GATC_C Cuts at: 4432 4799 4432 Size: 367 6735 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2722 2961 3131 3930 4423 Size: 325 217 627 935 239 170 799 493 Cuts at: 4423 5878 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 935 799 627 493 325 239 217 170 BanII G_rGCy'C Cuts at: 1391 2153 2169 2210 2318 2366 2373 2423 2700 Size: 762 16 41 108 48 7 50 277 Cuts at: 2700 2765 2783 3917 4067 4421 1391 Size: 65 18 1134 150 354 4072 Fragments arranged by size: 4072 1134 762 354 277 150 108 65 50 48 41 18 16 7 BbeI G_GCGC'C Cuts at: 2726 3135 2726 Size: 409 6693 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3203 3692 928 Size: 971 1304 489 4338 Fragments arranged by size: 4338 1304 971 489 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3133 3167 5293 5327 1903 Size: 34 1196 34 2126 34 3678 Fragments arranged by size: 3678 2126 1196 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2075 4012 4994 6204 2075 Size: 1937 982 1210 2973 Fragments arranged by size: 2973 1937 1210 982 BclI T'GATC_A Cuts at: 1548 1548 Size: 7102 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2173 2195 5713 Size: 107 122 71 631 605 501 22 3518 Cuts at: 5713 136 Size: 1525 Fragments arranged by size: 3518 1525 631 605 501 122 107 71 22 BglII A'GATC_T Cuts at: 7097 7097 Size: 7102 BlpI GC'TnA_GC Cuts at: 3732 4519 3732 Size: 787 6315 Bme1580I G_kGCm'C Cuts at: 1790 2153 2169 2210 2366 2423 2495 2765 2813 Size: 363 16 41 156 57 72 270 48 Cuts at: 2813 3452 3524 3933 5163 6319 1790 Size: 639 72 409 1230 1156 2573 Fragments arranged by size: 2573 1230 1156 639 409 363 270 156 72 72 57 48 41 16 BmgBI CAC'GTC Cuts at: 1318 2302 1318 Size: 984 6118 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4309 4833 5791 Size: 416 53 83 399 24 2892 524 958 Cuts at: 5791 442 Size: 1753 Fragments arranged by size: 2892 1753 958 524 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2693 1056 Size: 1637 5465 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3936 3936 Size: 7102 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2357 4126 5762 1239 Size: 1118 1769 1636 2579 Fragments arranged by size: 2579 1769 1636 1118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2939 3029 4072 5227 6092 6390 2939 Size: 90 1043 1155 865 298 3651 Fragments arranged by size: 3651 1155 1043 865 298 90 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2300 3340 4090 5765 882 Size: 617 801 1040 750 1675 2219 Fragments arranged by size: 2219 1675 1040 801 750 617 BsaAI yAC'GTr Cuts at: 493 1204 4893 493 Size: 711 3689 2702 Fragments arranged by size: 3689 2702 711 BsaBI GATnn'nnATC Cuts at: 7096 7096 Size: 7102 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2541 2723 3132 4909 Size: 53 83 186 936 1008 182 409 1777 Cuts at: 4909 5291 5926 275 Size: 382 635 1451 Fragments arranged by size: 1777 1451 1008 936 635 409 382 186 182 83 53 BsaWI w'CCGG_w Cuts at: 2318 3435 5535 5933 6207 6354 6765 2318 Size: 1117 2100 398 274 147 411 2655 Fragments arranged by size: 2655 2100 1117 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3065 3095 3578 3608 4189 Size: 30 165 30 1662 30 483 30 581 Cuts at: 4189 4219 1178 Size: 30 4061 Fragments arranged by size: 4061 1662 581 483 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2198 2363 2747 3116 3637 3996 1186 Size: 1012 165 384 369 521 359 4292 Fragments arranged by size: 4292 1012 521 384 369 359 165 BseYI C'CCAG_C Cuts at: 2598 2630 2776 3761 4169 6305 6801 2598 Size: 32 146 985 408 2136 496 2899 Fragments arranged by size: 2899 2136 985 496 408 146 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6529 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2083 4552 5313 6341 1540 Size: 275 186 82 2469 761 1028 2301 Fragments arranged by size: 2469 2301 1028 761 275 186 82 BsiHKAI G_wGCw'C Cuts at: 3220 3917 4067 4217 5163 5248 6319 3220 Size: 697 150 150 946 85 1071 4003 Fragments arranged by size: 4003 1071 946 697 150 150 85 BsmI GAATG_Cn' Cuts at: 4613 4706 4613 Size: 93 7009 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2153 2169 2210 2318 2366 2373 2423 Size: 399 363 16 41 108 48 7 50 Cuts at: 2423 2495 2700 2765 2783 2813 3220 3452 3524 Size: 72 205 65 18 30 407 232 72 Cuts at: 3524 3917 3933 4067 4217 4421 5163 5248 6319 Size: 393 16 134 150 204 742 85 1071 Cuts at: 6319 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 407 399 393 363 232 205 204 150 134 108 85 72 72 65 50 48 41 30 18 16 16 7 BspEI T'CCGG_A Cuts at: 6765 6765 Size: 7102 BspHI T'CATG_A Cuts at: 4991 6721 4991 Size: 1730 5372 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2526 2874 2973 3333 3615 4457 844 Size: 1682 348 99 360 282 842 3489 Fragments arranged by size: 3489 1682 842 360 348 282 99 BsrBI CCG'CTC Cuts at: 1394 1823 2388 2591 4989 1394 Size: 429 565 203 2398 3507 Fragments arranged by size: 3507 2398 565 429 203 BsrDI GCAATG_nn' Cuts at: 65 1248 3854 5597 5771 65 Size: 1183 2606 1743 174 1396 Fragments arranged by size: 2606 1743 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2107 3338 5746 5933 1700 Size: 110 297 1231 2408 187 2869 Fragments arranged by size: 2869 2408 1231 297 187 110 BsrGI T'GTAC_A Cuts at: 96 3036 96 Size: 2940 4162 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7102 BssSI C'ACGA_G Cuts at: 3478 5162 6174 3478 Size: 1684 1012 4406 Fragments arranged by size: 4406 1684 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7102 BstBI TT'CG_AA Cuts at: 3153 5906 3153 Size: 2753 4349 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7102 BstXI CCAn_nnnn'nTGG Cuts at: 2574 2574 Size: 7102 BstYI r'GATC_y Cuts at: 1972 3721 4112 4432 4799 5184 5201 6653 6739 Size: 1749 391 320 367 385 17 1452 86 Cuts at: 6739 6751 7097 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1749 1452 391 385 367 346 320 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4155 4155 Size: 7102 BtgI C'CryG_G Cuts at: 513 1065 2004 2070 2310 2400 2436 2478 2767 Size: 552 939 66 240 90 36 42 289 Cuts at: 2767 4345 513 Size: 1578 3270 Fragments arranged by size: 3270 1578 939 552 289 240 90 66 42 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6363 Fragments arranged by size: 6363 683 56 BtsI GCAGTG_nn' Cuts at: 2425 3772 4618 5412 5432 2425 Size: 1347 846 794 20 4095 Fragments arranged by size: 4095 1347 846 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7102 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7067 DraI TTT'AAA Cuts at: 4406 4406 Size: 7102 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6109 809 Size: 507 4793 1802 Fragments arranged by size: 4793 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2080 2113 2553 Size: 54 1425 50 275 18 250 33 440 Cuts at: 2553 2748 3990 4044 4128 4549 5438 7067 8 Size: 195 1242 54 84 421 889 1629 43 Fragments arranged by size: 1629 1425 1242 889 440 421 275 250 195 84 54 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2080 4549 1537 Size: 275 268 2469 4090 Fragments arranged by size: 4090 2469 275 268 EarI CTCTTCn'nnn_ Cuts at: 1353 3668 3827 5032 1353 Size: 2315 159 1205 3423 Fragments arranged by size: 3423 2315 1205 159 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2452 2753 5689 6063 6209 387 Size: 1064 138 863 301 2936 374 146 1280 Fragments arranged by size: 2936 1280 1064 863 374 301 146 138 EcoICRI GAG'CTC Cuts at: 3915 4065 3915 Size: 150 6952 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2357 2876 4126 5165 5762 6549 6817 Size: 393 725 519 1250 1039 597 787 268 Cuts at: 6817 1239 Size: 1524 Fragments arranged by size: 1524 1250 1039 787 725 597 519 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3456 3546 3456 Size: 90 7012 EcoO109I rG'GnC_Cy Cuts at: 2096 2119 2149 2150 2206 2207 2235 2333 2419 Size: 23 30 1 56 1 28 98 86 Cuts at: 2419 2420 2515 2743 2762 2898 2096 Size: 1 95 228 19 136 6300 Fragments arranged by size: 6300 228 136 98 95 86 56 30 28 23 19 1 1 1 EcoRI G'AATT_C Cuts at: 4411 4789 4411 Size: 378 6724 FspI TGC'GCA Cuts at: 2415 5608 2415 Size: 3193 3909 HaeII r_GCGC'y Cuts at: 1678 2726 2822 3135 6249 1678 Size: 1048 96 313 3114 2531 Fragments arranged by size: 3114 2531 1048 313 96 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2224 2256 2660 2692 3800 Size: 32 135 32 652 32 404 32 1108 Cuts at: 3800 3802 3832 3834 3965 3997 4070 4102 4261 Size: 2 30 2 131 32 73 32 159 Cuts at: 4261 4293 5743 5775 5817 5849 1373 Size: 32 1450 32 42 32 2626 Fragments arranged by size: 2626 1450 1108 652 404 159 135 131 73 42 32 32 32 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 4446 4692 669 Size: 988 281 2508 246 3079 Fragments arranged by size: 3079 2508 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7102 HpaI GTT'AAC Cuts at: 4692 4692 Size: 7102 KasI G'GCGC_C Cuts at: 2722 3131 2722 Size: 409 6693 KpnI G_GTAC'C Cuts at: 4427 4427 Size: 7102 MfeI C'AATT_G Cuts at: 4701 4701 Size: 7102 MluI A'CGCG_T Cuts at: 6978 6978 Size: 7102 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2703 6216 6400 1305 Size: 1398 3513 184 2007 Fragments arranged by size: 3513 2007 1398 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2115 2555 3992 4130 10 Size: 54 1425 343 283 440 1437 138 2982 Fragments arranged by size: 2982 1437 1425 440 343 283 138 54 MslI CAynn'nnrTG Cuts at: 518 1166 2655 3032 3392 3429 5060 5419 5578 Size: 648 1489 377 360 37 1631 359 159 Cuts at: 5578 518 Size: 2042 Fragments arranged by size: 2042 1631 1489 648 377 360 359 159 37 MspA1I CmG'CkG Cuts at: 1606 2072 2312 2402 2438 2480 2769 2854 4518 Size: 466 240 90 36 42 289 85 1664 Cuts at: 4518 5195 6343 6588 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1664 1148 677 466 289 245 240 90 85 42 36 NaeI GCC'GGC Cuts at: 2109 2109 Size: 7102 NarI GG'CG_CC Cuts at: 2723 3132 2723 Size: 409 6693 NcoI C'CATG_G Cuts at: 513 1065 2004 4345 513 Size: 552 939 2341 3270 Fragments arranged by size: 3270 2341 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7102 NgoMIV G'CCGG_C Cuts at: 2107 2107 Size: 7102 NheI G'CTAG_C Cuts at: 1052 2689 1052 Size: 1637 5465 NotI GC'GGCC_GC Cuts at: 4549 4549 Size: 7102 NspI r_CATG'y Cuts at: 1132 1229 3397 4460 6005 1132 Size: 97 2168 1063 1545 2229 Fragments arranged by size: 2229 2168 1545 1063 97 PasI CC'CwG_GG Cuts at: 1348 2758 2914 1348 Size: 1410 156 5536 Fragments arranged by size: 5536 1410 156 PciI A'CATG_T Cuts at: 6001 6001 Size: 7102 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7102 PmeI GTTT'AAAC Cuts at: 4406 4406 Size: 7102 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3065 3097 5171 5203 6684 6716 3065 Size: 32 2074 32 1481 32 3451 Fragments arranged by size: 3451 2074 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2119 2515 2119 Size: 396 6706 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7102 PsiI TTA'TAA Cuts at: 4672 4672 Size: 7102 PspOMI G'GGCC_C Cuts at: 2149 2165 2206 2362 2419 2761 2149 Size: 16 41 156 57 342 6490 Fragments arranged by size: 6490 342 156 57 41 16 PstI C_TGCA'G Cuts at: 830 2970 3287 3612 4454 830 Size: 2140 317 325 842 3478 Fragments arranged by size: 3478 2140 842 325 317 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7102 PvuII CAG'CTG Cuts at: 2854 2854 Size: 7102 RsrII CG'GwC_CG Cuts at: 2676 2676 Size: 7102 SacI G_AGCT'C Cuts at: 3917 4067 3917 Size: 150 6952 SacII CC_GC'GG Cuts at: 2073 2313 2403 2439 2481 2770 2073 Size: 240 90 36 42 289 6405 Fragments arranged by size: 6405 289 240 90 42 36 SalI G'TCGA_C Cuts at: 1936 4444 1936 Size: 2508 4594 SbfI CC_TGCA'GG Cuts at: 3612 4454 3612 Size: 842 6260 ScaI AGT'ACT Cuts at: 3844 5350 7062 3844 Size: 1506 1712 3884 Fragments arranged by size: 3884 1712 1506 SfcI C'TryA_G Cuts at: 826 1045 2042 2966 3283 3608 4450 5585 6266 Size: 219 997 924 317 325 842 1135 681 Cuts at: 6266 6457 826 Size: 191 1471 Fragments arranged by size: 1471 1135 997 924 842 681 325 317 219 191 SfoI GGC'GCC Cuts at: 2724 3133 2724 Size: 409 6693 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7102 SgrAI Cr'CCGG_yG Cuts at: 1700 3338 1700 Size: 1638 5464 SmaI CCC'GGG Cuts at: 1872 2147 2170 2367 2655 4429 1872 Size: 275 23 197 288 1774 4545 Fragments arranged by size: 4545 1774 288 275 197 23 SmlI C'TyrA_G Cuts at: 820 1017 1943 2954 3044 3406 4087 5206 6107 Size: 197 926 1011 90 362 681 1119 901 Cuts at: 6107 6369 820 Size: 262 1553 Fragments arranged by size: 1553 1119 1011 926 901 681 362 262 197 90 SnaBI TAC'GTA Cuts at: 493 4893 493 Size: 4400 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7102 SphI G_CATG'C Cuts at: 1132 4460 1132 Size: 3328 3774 SrfI GCCC'GGGC Cuts at: 2170 2170 Size: 7102 SspI AAT'ATT Cuts at: 5 52 5026 5 Size: 47 4974 2081 Fragments arranged by size: 4974 2081 47 StuI AGG'CCT Cuts at: 1517 3166 1517 Size: 1649 5453 StyI C'CwwG_G Cuts at: 513 1065 2004 2355 4345 4541 513 Size: 552 939 351 1990 196 3074 Fragments arranged by size: 3074 1990 939 552 351 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2282 2906 3775 4183 5129 5146 5299 Size: 1255 702 624 869 408 946 17 153 Cuts at: 5299 5484 5929 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 946 869 702 624 445 408 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3036 3842 5348 Size: 274 80 33 51 1443 1059 806 1506 Cuts at: 5348 7060 96 Size: 1712 138 Fragments arranged by size: 1712 1506 1443 1059 806 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2417 2449 7062 7094 2417 Size: 32 4613 32 2425 Fragments arranged by size: 4613 2425 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7102 XbaI T'CTAG_A Cuts at: 4438 4438 Size: 7102 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2571 2571 Size: 7102 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7102 XmaI C'CCGG_G Cuts at: 1870 2145 2168 2365 2653 4427 1870 Size: 275 23 197 288 1774 4545 Fragments arranged by size: 4545 1774 288 275 197 23 XmnI GAAnn'nnTTC Cuts at: 3080 3473 5231 3080 Size: 393 1758 4951 Fragments arranged by size: 4951 1758 393 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4910 276 Size: 53 83 186 936 3376 2468 Fragments arranged by size: 3376 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI SanDI SapI SexAI SfiI SwaI