Restriction map for
pFN21AE3848
(Linear) (Six-base) MAPSORT of: KIEE3848.seq Check: 7027 from: 1 to: 2397
KIEE3848.orf
With 182 enzymes: SgfI *
December 5, 2014 12:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 869 968 1328 1610 2397
Size: 869 99 360 282 787
Fragments arranged by size:
869 787 360 282 99
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 2397
Size: 871 1526
AflII C'TTAA_G
Cuts at: 0 1401 2397
Size: 1401 996
AhdI GACnn_n'nnGTC
Cuts at: 0 1974 2397
Size: 1974 423
AleI CACnn'nnGTG
Cuts at: 0 650 1027 2397
Size: 650 377 1370
Fragments arranged by size:
1370 650 377
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1949 2397
Size: 1949 448
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1060 1092 2397
Size: 1060 32 1305
Fragments arranged by size:
1305 1060 32
AlwNI CAG_nnn'CTG
Cuts at: 0 331 513 1254 2397
Size: 331 182 741 1143
Fragments arranged by size:
1143 741 331 182
ApaI G_GGCC'C
Cuts at: 0 148 164 205 361 418 760 2397
Size: 148 16 41 156 57 342 1637
Fragments arranged by size:
1637 342 156 148 57 41 16
ApoI r'AATT_y
Cuts at: 0 1313 1743 2397
Size: 1313 430 654
Fragments arranged by size:
1313 654 430
AvaI C'yCGr_G
Cuts at: 0 140 163 360 648 808 1153 2397
Size: 140 23 197 288 160 345 1244
Fragments arranged by size:
1244 345 288 197 160 140 23
BanI G'GyrC_C
Cuts at: 0 717 956 1126 1925 2397
Size: 717 239 170 799 472
Fragments arranged by size:
799 717 472 239 170
BanII G_rGCy'C
Cuts at: 0 148 164 205 313 361 368 418 695
Size: 148 16 41 108 48 7 50 277
Cuts at: 695 760 778 1912 2062 2397
Size: 65 18 1134 150 335
Fragments arranged by size:
1134 335 277 150 148 108 65 50
48 41 18 16 7
BbeI G_GCGC'C
Cuts at: 0 721 1130 2397
Size: 721 409 1267
Fragments arranged by size:
1267 721 409
BbsI GAAGACnn'nnnn_
Cuts at: 0 1198 1687 2397
Size: 1198 489 710
Fragments arranged by size:
1198 710 489
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1128 1162 2397
Size: 1128 34 1235
Fragments arranged by size:
1235 1128 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 70 2007 2397
Size: 70 1937 390
Fragments arranged by size:
1937 390 70
BglI GCCn_nnn'nGGC
Cuts at: 0 168 190 2397
Size: 168 22 2207
Fragments arranged by size:
2207 168 22
BlpI GC'TnA_GC
Cuts at: 0 1727 2397
Size: 1727 670
Bme1580I G_kGCm'C
Cuts at: 0 148 164 205 361 418 490 760 808
Size: 148 16 41 156 57 72 270 48
Cuts at: 808 1447 1519 1928 2397
Size: 639 72 409 469
Fragments arranged by size:
639 469 409 270 156 148 72 72
57 48 41 16
BmgBI CAC'GTC
Cuts at: 0 297 2397
Size: 297 2100
BmrI ACTGGGnnnn_n'
Cuts at: 0 2304 2397
Size: 2304 93
BmtI G_CTAG'C
Cuts at: 0 688 2397
Size: 688 1709
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1931 2397
Size: 1931 466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 2121 2397
Size: 352 1769 276
Fragments arranged by size:
1769 352 276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 934 1024 2067 2397
Size: 934 90 1043 330
Fragments arranged by size:
1043 934 330 90
BsaI GGTCTCn'nnnn_
Cuts at: 0 295 1335 2085 2397
Size: 295 1040 750 312
Fragments arranged by size:
1040 750 312 295
BsaHI Gr'CG_yC
Cuts at: 0 536 718 1127 2397
Size: 536 182 409 1270
Fragments arranged by size:
1270 536 409 182
BsaWI w'CCGG_w
Cuts at: 0 313 1430 2397
Size: 313 1117 967
Fragments arranged by size:
1117 967 313
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1060 1090 1573 1603 2184 2214 2397
Size: 1060 30 483 30 581 30 183
Fragments arranged by size:
1060 581 483 183 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 358 742 1111 1632 1991 2397
Size: 193 165 384 369 521 359 406
Fragments arranged by size:
521 406 384 369 359 193 165
BseYI C'CCAG_C
Cuts at: 0 593 625 771 1756 2164 2397
Size: 593 32 146 985 408 233
Fragments arranged by size:
985 593 408 233 146 32
BsiEI CG_ry'CG
Cuts at: 0 78 2397
Size: 78 2319
BsiHKAI G_wGCw'C
Cuts at: 0 1215 1912 2062 2212 2397
Size: 1215 697 150 150 185
Fragments arranged by size:
1215 697 185 150 150
Bsp1286I G_dGCh'C
Cuts at: 0 148 164 205 313 361 368 418 490
Size: 148 16 41 108 48 7 50 72
Cuts at: 490 695 760 778 808 1215 1447 1519 1912
Size: 205 65 18 30 407 232 72 393
Cuts at: 1912 1928 2062 2212 2397
Size: 16 134 150 185
Fragments arranged by size:
407 393 232 205 185 150 148 134
108 72 72 65 50 48 41 30
18 16 16 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 521 869 968 1328 1610 2397
Size: 521 348 99 360 282 787
Fragments arranged by size:
787 521 360 348 282 99
BsrBI CCG'CTC
Cuts at: 0 383 586 2397
Size: 383 203 1811
Fragments arranged by size:
1811 383 203
BsrDI GCAATG_nn'
Cuts at: 0 1849 2397
Size: 1849 548
BsrFI r'CCGG_y
Cuts at: 0 102 1333 2397
Size: 102 1231 1064
Fragments arranged by size:
1231 1064 102
BsrGI T'GTAC_A
Cuts at: 0 1031 2397
Size: 1031 1366
BssSI C'ACGA_G
Cuts at: 0 1473 2397
Size: 1473 924
BstBI TT'CG_AA
Cuts at: 0 1148 2397
Size: 1148 1249
BstXI CCAn_nnnn'nTGG
Cuts at: 0 569 2397
Size: 569 1828
BstYI r'GATC_y
Cuts at: 0 1716 2107 2397
Size: 1716 391 290
Fragments arranged by size:
1716 391 290
Bsu36I CC'TnA_GG
Cuts at: 0 2150 2397
Size: 2150 247
BtgI C'CryG_G
Cuts at: 0 65 305 395 431 473 762 2340 2397
Size: 65 240 90 36 42 289 1578 57
Fragments arranged by size:
1578 289 240 90 65 57 42 36
BtsI GCAGTG_nn'
Cuts at: 0 420 1767 2397
Size: 420 1347 630
Fragments arranged by size:
1347 630 420
EaeI y'GGCC_r
Cuts at: 0 75 108 548 743 1985 2039 2123 2397
Size: 75 33 440 195 1242 54 84 274
Fragments arranged by size:
1242 440 274 195 84 75 54 33
EagI C'GGCC_G
Cuts at: 0 75 2397
Size: 75 2322
EarI CTCTTCn'nnn_
Cuts at: 0 1663 1822 2397
Size: 1663 159 575
Fragments arranged by size:
1663 575 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 447 748 2397
Size: 447 301 1649
Fragments arranged by size:
1649 447 301
EcoICRI GAG'CTC
Cuts at: 0 1910 2060 2397
Size: 1910 150 337
Fragments arranged by size:
1910 337 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 871 2121 2397
Size: 352 519 1250 276
Fragments arranged by size:
1250 519 352 276
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1451 1541 2397
Size: 1451 90 856
Fragments arranged by size:
1451 856 90
EcoO109I rG'GnC_Cy
Cuts at: 0 91 114 144 145 201 202 230 328
Size: 91 23 30 1 56 1 28 98
Cuts at: 328 414 415 510 738 757 893 2397
Size: 86 1 95 228 19 136 1504
Fragments arranged by size:
1504 228 136 98 95 91 86 56
30 28 23 19 1 1 1
FspI TGC'GCA
Cuts at: 0 410 2397
Size: 410 1987
HaeII r_GCGC'y
Cuts at: 0 721 817 1130 2397
Size: 721 96 313 1267
Fragments arranged by size:
1267 721 313 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 219 251 655 687 1795 1797 1827 1829
Size: 219 32 404 32 1108 2 30 2
Cuts at: 1829 1960 1992 2065 2097 2256 2288 2397
Size: 131 32 73 32 159 32 109
Fragments arranged by size:
1108 404 219 159 131 109 73 32
32 32 32 32 30 2 2
KasI G'GCGC_C
Cuts at: 0 717 1126 2397
Size: 717 409 1271
Fragments arranged by size:
1271 717 409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 2397
Size: 698 1699
MscI TGG'CCA
Cuts at: 0 110 550 1987 2125 2397
Size: 110 440 1437 138 272
Fragments arranged by size:
1437 440 272 138 110
MslI CAynn'nnrTG
Cuts at: 0 650 1027 1387 1424 2397
Size: 650 377 360 37 973
Fragments arranged by size:
973 650 377 360 37
MspA1I CmG'CkG
Cuts at: 0 67 307 397 433 475 764 849 2397
Size: 67 240 90 36 42 289 85 1548
Fragments arranged by size:
1548 289 240 90 85 67 42 36
NaeI GCC'GGC
Cuts at: 0 104 2397
Size: 104 2293
NarI GG'CG_CC
Cuts at: 0 718 1127 2397
Size: 718 409 1270
Fragments arranged by size:
1270 718 409
NcoI C'CATG_G
Cuts at: 0 2340 2397
Size: 2340 57
NgoMIV G'CCGG_C
Cuts at: 0 102 2397
Size: 102 2295
NheI G'CTAG_C
Cuts at: 0 684 2397
Size: 684 1713
NspI r_CATG'y
Cuts at: 0 1392 2397
Size: 1392 1005
PasI CC'CwG_GG
Cuts at: 0 753 909 2397
Size: 753 156 1488
Fragments arranged by size:
1488 753 156
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1060 1092 2397
Size: 1060 32 1305
Fragments arranged by size:
1305 1060 32
PpuMI rG'GwC_Cy
Cuts at: 0 114 510 2397
Size: 114 396 1887
Fragments arranged by size:
1887 396 114
PspOMI G'GGCC_C
Cuts at: 0 144 160 201 357 414 756 2397
Size: 144 16 41 156 57 342 1641
Fragments arranged by size:
1641 342 156 144 57 41 16
PstI C_TGCA'G
Cuts at: 0 965 1282 1607 2397
Size: 965 317 325 790
Fragments arranged by size:
965 790 325 317
PvuII CAG'CTG
Cuts at: 0 849 2397
Size: 849 1548
RsrII CG'GwC_CG
Cuts at: 0 671 2397
Size: 671 1726
SacI G_AGCT'C
Cuts at: 0 1912 2062 2397
Size: 1912 150 335
Fragments arranged by size:
1912 335 150
SacII CC_GC'GG
Cuts at: 0 68 308 398 434 476 765 2397
Size: 68 240 90 36 42 289 1632
Fragments arranged by size:
1632 289 240 90 68 42 36
SbfI CC_TGCA'GG
Cuts at: 0 1607 2397
Size: 1607 790
ScaI AGT'ACT
Cuts at: 0 1839 2397
Size: 1839 558
SfcI C'TryA_G
Cuts at: 0 37 961 1278 1603 2397
Size: 37 924 317 325 794
Fragments arranged by size:
924 794 325 317 37
SfoI GGC'GCC
Cuts at: 0 719 1128 2397
Size: 719 409 1269
Fragments arranged by size:
1269 719 409
SgrAI Cr'CCGG_yG
Cuts at: 0 1333 2397
Size: 1333 1064
SmaI CCC'GGG
Cuts at: 0 142 165 362 650 2397
Size: 142 23 197 288 1747
Fragments arranged by size:
1747 288 197 142 23
SmlI C'TyrA_G
Cuts at: 0 949 1039 1401 2082 2397
Size: 949 90 362 681 315
Fragments arranged by size:
949 681 362 315 90
SrfI GCCC'GGGC
Cuts at: 0 165 2397
Size: 165 2232
StuI AGG'CCT
Cuts at: 0 1161 2397
Size: 1161 1236
StyI C'CwwG_G
Cuts at: 0 350 2340 2397
Size: 350 1990 57
Fragments arranged by size:
1990 350 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 277 901 1770 2178 2397
Size: 277 624 869 408 219
Fragments arranged by size:
869 624 408 277 219
TatI w'GTAC_w
Cuts at: 0 1031 1837 2397
Size: 1031 806 560
Fragments arranged by size:
1031 806 560
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 412 444 2397
Size: 412 32 1953
Fragments arranged by size:
1953 412 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 566 2397
Size: 566 1831
XmaI C'CCGG_G
Cuts at: 0 140 163 360 648 2397
Size: 140 23 197 288 1749
Fragments arranged by size:
1749 288 197 140 23
XmnI GAAnn'nnTTC
Cuts at: 0 1075 1468 2397
Size: 1075 393 929
Fragments arranged by size:
1075 929 393
Enzymes that do cut:
AarI AcuI AflII AhdI AleI AlfI AloI AlwNI
ApaI ApoI AvaI BanI BanII BbeI BbsI BcgI
BciVI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstBI BstXI BstYI Bsu36I BtgI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PpiI PpuMI
PspOMI PstI PvuII RsrII SacI SacII SbfI ScaI
SfcI SfoI SgrAI SmaI SmlI SrfI StuI StyI
TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BclI
BfrBI BglII Bpu10I BsaAI BsaBI BsgI BsiWI BsmI
BsmBI BspEI BspHI BssHII BstAPI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
NdeI NotI NruI NsiI PacI PciI PflMI PfoI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI SalI
SanDI SapI SexAI SfiI SgfI SnaBI SpeI SphI
SspI SwaI Tth111I XbaI XhoI ZraI