################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiWI BsmAI BsmBI BsoBI BspDI BspEI BspMI BsrBI BssHII BssSI Bst1107I BstAPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoRI EcoRV EcoT22I FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Alw44I 801 GTGCAC G^TGCAC AlwNI 860 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 801 GTGCAC G^TGCAC Asp700I 1012 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 801 GTGCAC G[AT]GC[AT]^C BalI 167 TGGCCA TGG^CCA BpmI 737 CTCCAG CTGGAG(16/14) BsgI 633 CTGCAC GTGCAG(16/14) BsiEI 124 CGGCCG CG[GA][CT]^CG BsiHKAI 801 GTGCAC G[AT]GC[AT]^C Bsp1407I 1208 TGTACA T^GTACA BspHI 434 TCATGA T^CATGA BsrFI 126 GCCGGT [GA]^CCGG[CT] BsrGI 1208 TGTACA T^GTACA BstBI 692 TTCGAA TT^CGAA BstEII 1144 GGTGACC G^GT[ACGT]ACC BstPI 1144 GGTGACC G^GT[ACGT]ACC CfoI 46 GCGC GCG^C Cfr10I 126 GCCGGT [GA]^CCGG[CT] DraI 903 TTTAAA TTT^AAA DraII 861 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 1149 CCATGG C^C[GA][CT]GG EagI 124 CGGCCG C^GGCCG EclXI 124 CGGCCG C^GGCCG Eco52I 124 CGGCCG C^GGCCG EcoNI 733 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 861 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 1144 GGTGACC G^GT[ACGT]ACC HaeII 45 GGCGCT [GA]GCGC^[CT] HapII 127 CCGG C^CGG HhaI 46 GCGC GCG^C HinP1I 46 GCGC G^CGC HpaII 127 CCGG C^CGG MaeII 239 ACGT A^CGT MluNI 167 TGGCCA TGG^CCA MscI 167 TGGCCA TGG^CCA MspI 127 CCGG C^CGG NcoI 1149 CCATGG C^CATGG NdeI 587 CATATG CA^TATG NspV 692 TTCGAA TT^CGAA PpuMI 861 AGGACCT [GA]G^G[AT]CC[CT] RcaI 434 TCATGA T^CATGA SfuI 692 TTCGAA TT^CGAA SspBI 1208 TGTACA T^GTACA TaqI 693 TCGA T^CGA TthHB8I 693 TCGA T^CGA XcmI 1500 CCAGGTTGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1012 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 916 AAATTT [GA]^AATT[CT] 1126 AAATTT AflIII 1038 ACATGT A^C[GA][CT]GT 1158 ACATGT ApoI 916 AAATTT [GA]^AATT[CT] 1126 AAATTT BanII 669 GGGCTC G[GA]GC[CT]^C 782 GAGCCC BbsI 829 GAAGAC GAAGAC(2/6) 1426 GTCTTC BglII 563 AGATCT A^GATCT 972 AGATCT BpuAI 829 GAAGAC GAAGAC(2/6) 1426 GTCTTC BspLU11I 1038 ACATGT A^CATGT 1158 ACATGT BstXI 470 CCACTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1223 CCATTGGACTGG BstYI 563 AGATCT [GA]^GATC[CT] 972 AGATCT FauI 20 CCCGC CCCGC(4/6) 42 GCGGG HindIII 316 AAGCTT A^AGCTT 395 AAGCTT MflI 563 AGATCT [GA]^GATC[CT] 972 AGATCT MslI 471 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 690 CATTCGAATG MspA1I 163 CAGCTG C[AC]G^C[GT]G 644 CAGCTG NspI 1038 ACATGT [GA]CATG^[CT] 1158 ACATGT PflMI 1500 CCAGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1548 CCAGTACTTGG PvuII 163 CAGCTG CAG^CTG 644 CAGCTG ScaI 1061 AGTACT AGT^ACT 1550 AGTACT SfcI 305 CTGTAG C^T[GA][CT]AG 525 CTGTAG TfiI 230 GAATC G^A[AT]TC 550 GAATC Van91I 1500 CCAGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1548 CCAGTACTTGG XhoII 563 AGATCT [GA]^GATC[CT] 972 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BanI BbvI BclI BfaI BmyI BsaJI BsiYI BslI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI Tru9I TseI Tsp45I Tsp509I TspRI