(Circular) (Six-base) MAPSORT of: pFN21AE6760.seq Check: 6253 from: 1 to: 6301 pFN21AE6760 6301 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4111 278 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3644 1937 Size: 1707 4594 Acc65I G'GTAC_C Cuts at: 3622 3622 Size: 6301 AclI AA'CG_TT Cuts at: 1563 4428 4801 1563 Size: 2865 373 3063 Fragments arranged by size: 3063 2865 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2513 2853 3074 3418 4364 5748 6016 1632 Size: 881 340 221 344 946 1384 268 1917 Fragments arranged by size: 1917 1384 946 881 344 340 268 221 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6104 AflIII A'CryG_T Cuts at: 1203 3043 3163 5200 6177 1203 Size: 1840 120 2037 977 1327 Fragments arranged by size: 2037 1840 1327 977 120 AgeI A'CCGG_T Cuts at: 5132 5132 Size: 6301 AhdI GACnn_n'nnGTC Cuts at: 5030 5030 Size: 6301 AleI CACnn'nnGTG Cuts at: 1166 2480 1166 Size: 1314 4987 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2671 2717 1184 Size: 1487 46 4768 Fragments arranged by size: 4768 1487 46 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3021 3053 3021 Size: 32 6269 AlwNI CAG_nnn'CTG Cuts at: 2870 4044 5616 2870 Size: 1174 1572 3555 Fragments arranged by size: 3555 1572 1174 ApaLI G'TGCA_C Cuts at: 2806 4358 5514 2806 Size: 1552 1156 3593 Fragments arranged by size: 3593 1552 1156 ApoI r'AATT_y Cuts at: 1493 2921 3131 3610 3836 3988 6097 1493 Size: 1428 210 479 226 152 2109 1697 Fragments arranged by size: 2109 1697 1428 479 226 210 152 AseI AT'TA_AT Cuts at: 160 1032 4855 160 Size: 872 3823 1606 Fragments arranged by size: 3823 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 AvaI C'yCGr_G Cuts at: 1870 1943 3626 1870 Size: 73 1683 4545 Fragments arranged by size: 4545 1683 73 BamHI G'GATC_C Cuts at: 3631 3998 3631 Size: 367 5934 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2504 3195 3376 3622 Size: 325 217 627 221 496 691 181 246 Cuts at: 3622 5077 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 691 627 496 325 246 221 217 181 BanII G_rGCy'C Cuts at: 1391 2678 2791 3620 1391 Size: 1287 113 829 4072 Fragments arranged by size: 4072 1287 829 113 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2841 3424 928 Size: 971 942 583 3805 Fragments arranged by size: 3805 971 942 583 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4492 4526 1903 Size: 34 2555 34 3678 Fragments arranged by size: 3678 2555 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4193 5403 4193 Size: 1210 5091 BclI T'GATC_A Cuts at: 1548 2256 3186 3529 1548 Size: 708 930 343 4320 Fragments arranged by size: 4320 930 708 343 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4912 136 Size: 107 122 71 631 605 3240 1525 Fragments arranged by size: 3240 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2568 2977 6296 2568 Size: 409 3319 2573 Fragments arranged by size: 3319 2573 409 BlpI GC'TnA_GC Cuts at: 3718 3718 Size: 6301 Bme1580I G_kGCm'C Cuts at: 1790 2810 3442 4362 5518 1790 Size: 1020 632 920 1156 2573 Fragments arranged by size: 2573 1156 1020 920 632 BmgBI CAC'GTC Cuts at: 1318 2245 1318 Size: 927 5374 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3479 3547 4032 Size: 416 53 83 399 24 2062 68 485 Cuts at: 4032 4990 442 Size: 958 1753 Fragments arranged by size: 2062 1753 958 485 416 399 83 68 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6301 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2727 4961 1239 Size: 1488 2234 2579 Fragments arranged by size: 2579 2234 1488 Bpu10I CC'TnA_GC Cuts at: 3381 3381 Size: 6301 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4426 5291 5589 4426 Size: 865 298 5138 Fragments arranged by size: 5138 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4964 882 Size: 617 3465 2219 Fragments arranged by size: 3465 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4092 493 Size: 711 2888 2702 Fragments arranged by size: 2888 2702 711 BsaBI GATnn'nnATC Cuts at: 6295 6295 Size: 6301 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4108 4490 5125 275 Size: 53 83 186 936 2575 382 635 1451 Fragments arranged by size: 2575 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4734 5132 5406 5553 5964 4734 Size: 398 274 147 411 5071 Fragments arranged by size: 5071 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3021 3051 1178 Size: 30 165 30 1618 30 4428 Fragments arranged by size: 4428 1618 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6301 BseYI C'CCAG_C Cuts at: 3579 5504 6000 3579 Size: 1925 496 3880 Fragments arranged by size: 3880 1925 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2623 1163 Size: 573 887 4841 Fragments arranged by size: 4841 887 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2132 3751 4512 5540 1540 Size: 275 186 131 1619 761 1028 2301 Fragments arranged by size: 2301 1619 1028 761 275 186 131 BsiHKAI G_wGCw'C Cuts at: 2810 4362 4447 5518 2810 Size: 1552 85 1071 3593 Fragments arranged by size: 3593 1552 1071 85 BsmI GAATG_Cn' Cuts at: 2028 2424 2694 2706 3484 3812 3905 2028 Size: 396 270 12 778 328 93 4424 Fragments arranged by size: 4424 778 396 328 270 93 12 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2678 2791 2810 3442 3620 4362 4447 Size: 399 888 113 19 632 178 742 85 Cuts at: 4447 5518 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 888 742 632 399 178 113 85 19 BspEI T'CCGG_A Cuts at: 5964 5964 Size: 6301 BspHI T'CATG_A Cuts at: 2439 4190 5920 2439 Size: 1751 1730 2820 Fragments arranged by size: 2820 1751 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3656 844 Size: 2812 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4188 1394 Size: 429 2365 3507 Fragments arranged by size: 3507 2365 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2144 2457 3096 3274 4796 4970 65 Size: 1183 896 313 639 178 1522 174 1396 Fragments arranged by size: 1522 1396 1183 896 639 313 178 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2131 4945 5132 1700 Size: 110 321 2814 187 2869 Fragments arranged by size: 2869 2814 321 187 110 BsrGI T'GTAC_A Cuts at: 96 3213 96 Size: 3117 3184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6301 BssSI C'ACGA_G Cuts at: 4361 5373 4361 Size: 1012 5289 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6301 BstBI TT'CG_AA Cuts at: 2698 5105 2698 Size: 2407 3894 BstEII G'GTnAC_C Cuts at: 1184 3149 1184 Size: 1965 4336 BstXI CCAn_nnnn'nTGG Cuts at: 2482 3235 2482 Size: 753 5548 BstYI r'GATC_y Cuts at: 1972 2568 2977 3631 3998 4383 4400 5852 5938 Size: 596 409 654 367 385 17 1452 86 Cuts at: 5938 5950 6296 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 654 596 409 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3154 513 Size: 552 939 1150 3660 Fragments arranged by size: 3660 1150 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2306 487 Size: 683 56 1080 4482 Fragments arranged by size: 4482 1080 683 56 BtsI GCAGTG_nn' Cuts at: 3300 3817 4611 4631 3300 Size: 517 794 20 4970 Fragments arranged by size: 4970 794 517 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6301 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6266 DraI TTT'AAA Cuts at: 2910 3605 2910 Size: 695 5606 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5308 809 Size: 507 3992 1802 Fragments arranged by size: 3992 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2129 2172 2575 Size: 54 1425 50 275 18 299 43 403 Cuts at: 2575 3748 4637 6266 8 Size: 1173 889 1629 43 Fragments arranged by size: 1629 1425 1173 889 403 299 275 54 50 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2129 3748 1537 Size: 275 317 1619 4090 Fragments arranged by size: 4090 1619 317 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4231 1353 Size: 2878 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4888 5262 5408 387 Size: 1064 138 3299 374 146 1280 Fragments arranged by size: 3299 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2513 2727 2853 3074 3418 4364 4961 Size: 393 881 214 126 221 344 946 597 Cuts at: 4961 5748 6016 1239 Size: 787 268 1524 Fragments arranged by size: 1524 946 881 787 597 393 344 268 221 214 126 EcoNI CCTnn'n_nnAGG Cuts at: 2742 2742 Size: 6301 EcoO109I rG'GnC_Cy Cuts at: 2867 2867 Size: 6301 EcoRI G'AATT_C Cuts at: 3610 3988 3610 Size: 378 5923 FspI TGC'GCA Cuts at: 4807 4807 Size: 6301 HaeII r_GCGC'y Cuts at: 1678 2054 5448 1678 Size: 376 3394 2531 Fragments arranged by size: 3394 2531 376 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2859 2891 2933 2965 4942 Size: 32 135 32 1287 32 42 32 1977 Cuts at: 4942 4974 5016 5048 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1977 1287 135 42 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3645 3891 669 Size: 988 281 1707 246 3079 Fragments arranged by size: 3079 1707 988 281 246 HindIII A'AGCT_T Cuts at: 748 2321 2400 748 Size: 1573 79 4649 Fragments arranged by size: 4649 1573 79 HpaI GTT'AAC Cuts at: 3891 3891 Size: 6301 KpnI G_GTAC'C Cuts at: 3626 3626 Size: 6301 MfeI C'AATT_G Cuts at: 3900 3900 Size: 6301 MluI A'CGCG_T Cuts at: 6177 6177 Size: 6301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2209 3225 5415 5599 1305 Size: 904 1016 2190 184 2007 Fragments arranged by size: 2190 2007 1016 904 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2174 10 Size: 54 1425 343 342 4137 Fragments arranged by size: 4137 1425 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 2480 2699 4259 4618 4777 518 Size: 648 1314 219 1560 359 159 2042 Fragments arranged by size: 2042 1560 1314 648 359 219 159 MspA1I CmG'CkG Cuts at: 1606 2170 2651 3717 4394 5542 5787 1606 Size: 564 481 1066 677 1148 245 2120 Fragments arranged by size: 2120 1148 1066 677 564 481 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3154 513 Size: 552 939 1150 3660 Fragments arranged by size: 3660 1150 939 552 NdeI CA'TA_TG Cuts at: 387 2593 387 Size: 2206 4095 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6301 NotI GC'GGCC_GC Cuts at: 3748 3748 Size: 6301 NspI r_CATG'y Cuts at: 1132 1229 3047 3167 3659 5204 1132 Size: 97 1818 120 492 1545 2229 Fragments arranged by size: 2229 1818 1545 492 120 97 PasI CC'CwG_GG Cuts at: 1348 3442 1348 Size: 2094 4207 PciI A'CATG_T Cuts at: 3043 3163 5200 3043 Size: 120 2037 4144 Fragments arranged by size: 4144 2037 120 PflMI CCAn_nnn'nTGG Cuts at: 1108 3511 3559 1108 Size: 2403 48 3850 Fragments arranged by size: 3850 2403 48 PfoI T'CCnGG_A Cuts at: 3589 3589 Size: 6301 PmeI GTTT'AAAC Cuts at: 3605 3605 Size: 6301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3021 3053 4370 4402 5883 5915 3021 Size: 32 1317 32 1481 32 3407 Fragments arranged by size: 3407 1481 1317 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2867 2867 Size: 6301 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6301 PsiI TTA'TAA Cuts at: 3871 3871 Size: 6301 PstI C_TGCA'G Cuts at: 830 3653 830 Size: 2823 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6301 PvuII CAG'CTG Cuts at: 2170 2651 2170 Size: 481 5820 SalI G'TCGA_C Cuts at: 1936 3643 1936 Size: 1707 4594 SbfI CC_TGCA'GG Cuts at: 3653 3653 Size: 6301 ScaI AGT'ACT Cuts at: 3068 3557 4549 6261 3068 Size: 489 992 1712 3108 Fragments arranged by size: 3108 1712 992 489 SfcI C'TryA_G Cuts at: 826 1045 2310 2530 3649 4784 5465 5656 826 Size: 219 1265 220 1119 1135 681 191 1471 Fragments arranged by size: 1471 1265 1135 1119 681 220 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6301 SmaI CCC'GGG Cuts at: 1872 3628 1872 Size: 1756 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4405 5306 5568 820 Size: 197 926 2462 901 262 1553 Fragments arranged by size: 2462 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4092 493 Size: 3599 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6301 SphI G_CATG'C Cuts at: 1132 3659 1132 Size: 2527 3774 SspI AAT'ATT Cuts at: 5 52 4225 5 Size: 47 4173 2081 Fragments arranged by size: 4173 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6301 StyI C'CwwG_G Cuts at: 513 1065 2004 2374 2775 3154 3740 513 Size: 552 939 370 401 379 586 3074 Fragments arranged by size: 3074 939 586 552 401 379 370 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4328 4345 4498 4683 5128 325 Size: 1255 2748 17 153 185 445 1498 Fragments arranged by size: 2748 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2274 3066 3213 Size: 274 80 33 51 1443 297 792 147 Cuts at: 3213 3555 4547 6259 96 Size: 342 992 1712 138 Fragments arranged by size: 1712 1443 992 792 342 297 274 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6261 6293 6261 Size: 32 6269 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6301 XbaI T'CTAG_A Cuts at: 3637 3637 Size: 6301 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3512 3512 Size: 6301 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6301 XmaI C'CCGG_G Cuts at: 1870 3626 1870 Size: 1756 4545 XmnI GAAnn'nnTTC Cuts at: 3021 4430 3021 Size: 1409 4892 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4109 276 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI