(Linear) (Six-base) MAPSORT of: KIEE6760.seq Check: 9374 from: 1 to: 1596 KIEE6760.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 508 848 1069 1413 1596 Size: 508 340 221 344 183 Fragments arranged by size: 508 344 340 221 183 AflIII A'CryG_T Cuts at: 0 1038 1158 1596 Size: 1038 120 438 Fragments arranged by size: 1038 438 120 AleI CACnn'nnGTG Cuts at: 0 475 1596 Size: 475 1121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 666 712 1596 Size: 666 46 884 Fragments arranged by size: 884 666 46 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1016 1048 1596 Size: 1016 32 548 Fragments arranged by size: 1016 548 32 AlwNI CAG_nnn'CTG Cuts at: 0 865 1596 Size: 865 731 ApaLI G'TGCA_C Cuts at: 0 801 1596 Size: 801 795 ApoI r'AATT_y Cuts at: 0 916 1126 1596 Size: 916 210 470 Fragments arranged by size: 916 470 210 BanI G'GyrC_C Cuts at: 0 3 499 1190 1371 1596 Size: 3 496 691 181 225 Fragments arranged by size: 691 496 225 181 3 BanII G_rGCy'C Cuts at: 0 673 786 1596 Size: 673 113 810 Fragments arranged by size: 810 673 113 BbsI GAAGACnn'nnnn_ Cuts at: 0 836 1419 1596 Size: 836 583 177 Fragments arranged by size: 836 583 177 BclI T'GATC_A Cuts at: 0 251 1181 1524 1596 Size: 251 930 343 72 Fragments arranged by size: 930 343 251 72 BglII A'GATC_T Cuts at: 0 563 972 1596 Size: 563 409 624 Fragments arranged by size: 624 563 409 Bme1580I G_kGCm'C Cuts at: 0 805 1437 1596 Size: 805 632 159 Fragments arranged by size: 805 632 159 BmgBI CAC'GTC Cuts at: 0 240 1596 Size: 240 1356 BmrI ACTGGGnnnn_n' Cuts at: 0 1474 1542 1596 Size: 1474 68 54 Fragments arranged by size: 1474 68 54 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 722 1596 Size: 722 874 Bpu10I CC'TnA_GC Cuts at: 0 1376 1596 Size: 1376 220 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1016 1046 1596 Size: 1016 30 550 Fragments arranged by size: 1016 550 30 BseYI C'CCAG_C Cuts at: 0 1574 1596 Size: 1574 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 618 1596 Size: 618 978 BsiEI CG_ry'CG Cuts at: 0 127 1596 Size: 127 1469 BsiHKAI G_wGCw'C Cuts at: 0 805 1596 Size: 805 791 BsmI GAATG_Cn' Cuts at: 0 23 419 689 701 1479 1596 Size: 23 396 270 12 778 117 Fragments arranged by size: 778 396 270 117 23 12 Bsp1286I G_dGCh'C Cuts at: 0 673 786 805 1437 1596 Size: 673 113 19 632 159 Fragments arranged by size: 673 632 159 113 19 BspHI T'CATG_A Cuts at: 0 434 1596 Size: 434 1162 BsrDI GCAATG_nn' Cuts at: 0 139 452 1091 1269 1596 Size: 139 313 639 178 327 Fragments arranged by size: 639 327 313 178 139 BsrFI r'CCGG_y Cuts at: 0 126 1596 Size: 126 1470 BsrGI T'GTAC_A Cuts at: 0 1208 1596 Size: 1208 388 BstBI TT'CG_AA Cuts at: 0 693 1596 Size: 693 903 BstEII G'GTnAC_C Cuts at: 0 1144 1596 Size: 1144 452 BstXI CCAn_nnnn'nTGG Cuts at: 0 477 1230 1596 Size: 477 753 366 Fragments arranged by size: 753 477 366 BstYI r'GATC_y Cuts at: 0 563 972 1596 Size: 563 409 624 Fragments arranged by size: 624 563 409 BtgI C'CryG_G Cuts at: 0 1149 1596 Size: 1149 447 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 301 1596 Size: 301 1295 BtsI GCAGTG_nn' Cuts at: 0 1295 1596 Size: 1295 301 DraI TTT'AAA Cuts at: 0 905 1596 Size: 905 691 EaeI y'GGCC_r Cuts at: 0 124 167 570 1596 Size: 124 43 403 1026 Fragments arranged by size: 1026 403 124 43 EagI C'GGCC_G Cuts at: 0 124 1596 Size: 124 1472 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 508 722 848 1069 1413 1596 Size: 508 214 126 221 344 183 Fragments arranged by size: 508 344 221 214 183 126 EcoNI CCTnn'n_nnAGG Cuts at: 0 737 1596 Size: 737 859 EcoO109I rG'GnC_Cy Cuts at: 0 862 1596 Size: 862 734 HaeII r_GCGC'y Cuts at: 0 49 1596 Size: 49 1547 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 854 886 928 960 1596 Size: 854 32 42 32 636 Fragments arranged by size: 854 636 42 32 32 HindIII A'AGCT_T Cuts at: 0 316 395 1596 Size: 316 79 1201 Fragments arranged by size: 1201 316 79 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 204 1220 1596 Size: 204 1016 376 Fragments arranged by size: 1016 376 204 MscI TGG'CCA Cuts at: 0 169 1596 Size: 169 1427 MslI CAynn'nnrTG Cuts at: 0 475 694 1596 Size: 475 219 902 Fragments arranged by size: 902 475 219 MspA1I CmG'CkG Cuts at: 0 165 646 1596 Size: 165 481 950 Fragments arranged by size: 950 481 165 NcoI C'CATG_G Cuts at: 0 1149 1596 Size: 1149 447 NdeI CA'TA_TG Cuts at: 0 588 1596 Size: 588 1008 NspI r_CATG'y Cuts at: 0 1042 1162 1596 Size: 1042 120 434 Fragments arranged by size: 1042 434 120 PasI CC'CwG_GG Cuts at: 0 1437 1596 Size: 1437 159 PciI A'CATG_T Cuts at: 0 1038 1158 1596 Size: 1038 120 438 Fragments arranged by size: 1038 438 120 PflMI CCAn_nnn'nTGG Cuts at: 0 1506 1554 1596 Size: 1506 48 42 Fragments arranged by size: 1506 48 42 PfoI T'CCnGG_A Cuts at: 0 1584 1596 Size: 1584 12 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1016 1048 1596 Size: 1016 32 548 Fragments arranged by size: 1016 548 32 PpuMI rG'GwC_Cy Cuts at: 0 862 1596 Size: 862 734 PvuII CAG'CTG Cuts at: 0 165 646 1596 Size: 165 481 950 Fragments arranged by size: 950 481 165 ScaI AGT'ACT Cuts at: 0 1063 1552 1596 Size: 1063 489 44 Fragments arranged by size: 1063 489 44 SfcI C'TryA_G Cuts at: 0 305 525 1596 Size: 305 220 1071 Fragments arranged by size: 1071 305 220 StyI C'CwwG_G Cuts at: 0 369 770 1149 1596 Size: 369 401 379 447 Fragments arranged by size: 447 401 379 369 TatI w'GTAC_w Cuts at: 0 269 1061 1208 1550 1596 Size: 269 792 147 342 46 Fragments arranged by size: 792 342 269 147 46 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1507 1596 Size: 1507 89 XmnI GAAnn'nnTTC Cuts at: 0 1016 1596 Size: 1016 580 Enzymes that do cut: AcuI AflIII AleI AlfI AloI AlwNI ApaLI ApoI BanI BanII BbsI BclI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI BsrGI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI EaeI EagI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI NdeI NspI PasI PciI PflMI PfoI PpiI PpuMI PvuII ScaI SfcI StyI TatI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglI BlpI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiWI BsmBI BspEI BspMI BsrBI BssHII BssSI BstAPI BstZ17I Bsu36I ClaI CspCI DraIII DrdI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XhoI XmaI ZraI