Restriction map for
pFN21AE2033
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BciVI BclI BfrI
BglII BlnI BsaAI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII Bst1107I BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI Ksp632I MaeII MluI MroI
MslI NaeI NdeI NgoMIV NheI
NotI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SapI SbfI ScaI SexAI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 378 GTCGAC GT^[AC][GT]AC
AocI 322 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1618 AGCGCT AGC^GCT
ApaI 1540 GGGCCC GGGCC^C
Asp700I 186 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 145 CCCGAG C^[CT]CG[GA]G
BalI 527 TGGCCA TGG^CCA
BbeI 1164 GGCGCC GGCGC^C
BbsI 111 GAAGAC GAAGAC(2/6)
BbvCI 1258 CCTCAGC CCTCAGC(-5/-2)
BcgI 1827 CGACATCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 78 CCCGG CC^[GC]GG
BpuAI 111 GAAGAC GAAGAC(2/6)
BsaBI 540 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 1513 GTCCC GGGAC(10/14)
BsmI 840 GAATGC GAATGC(1/-1)
BsoBI 145 CCCGAG C^[CT]CG[GA]G
Bsp1407I 812 TGTACA T^GTACA
BspMI 668 ACCTGC ACCTGC(4/8)
BsrFI 1582 ACCGGC [GA]^CCGG[CT]
BsrGI 812 TGTACA T^GTACA
BssSI 1638 CACGAG CACGAG(-5/-1)
BstYI 1370 AGATCC [GA]^GATC[CT]
Bsu36I 322 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1582 ACCGGC [GA]^CCGG[CT]
DraIII 1625 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1618 AGCGCT AGC^GCT
Eco81I 322 CCTCAGG CC^T[ACGT]AGG
HgaI 376 GCGTC GACGC(5/10)
HincII 378 GTCGAC GT[CT]^[GA]AC
HindII 378 GTCGAC GT[CT]^[GA]AC
KasI 1164 GGCGCC G^GCGCC
MamI 540 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 111 GAAGA GAAGA(8/7)
MfeI 637 CAATTG C^AATTG
MflI 1370 AGATCC [GA]^GATC[CT]
MluNI 527 TGGCCA TGG^CCA
MscI 527 TGGCCA TGG^CCA
MunI 637 CAATTG C^AATTG
NarI 1164 GGCGCC GG^CGCC
NciI 78 CCCGG CC^[GC]GG
NcoI 955 CCATGG C^CATGG
NruI 936 TCGCGA TCG^CGA
PpuMI 1548 AGGACCC [GA]G^G[AT]CC[CT]
SalI 378 GTCGAC G^TCGAC
SfoI 1164 GGCGCC GGC^GCC
SmlI 474 CTTGAG C^T[CT][GA]AG
SspBI 812 TGTACA T^GTACA
StuI 456 AGGCCT AGG^CCT
XbaI 771 TCTAGA T^CTAGA
XhoII 1370 AGATCC [GA]^GATC[CT]
XmnI 186 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 375 GGCGTC G[GA]^CG[CT]C
1164 GGCGCC
AspHI 477 GAGCTC G[AT]GC[AT]^C
496 GAGCTC
AvaII 1549 GGACC G^G[AT]CC
1660 GGACC
BglI 1469 GCCCTGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1496 GCCCCATAGGC
BlpI 82 GCTGAGC GC^T[ACGT]AGC
1194 GCTGAGC
Bpu1102I 82 GCTGAGC GC^T[ACGT]AGC
1194 GCTGAGC
BsaHI 375 GGCGTC G[GA]^CG[CT]C
1164 GGCGCC
BseRI 897 CTCCTC GAGGAG(10/8)
130 GAGGAG
BsiHKAI 477 GAGCTC G[AT]GC[AT]^C
496 GAGCTC
BstBI 183 TTCGAA TT^CGAA
585 TTCGAA
BstF5I 273 CATCC GGATG(2/0)
780 CATCC
CelII 82 GCTGAGC GC^T[ACGT]AGC
1194 GCTGAGC
EaeI 527 TGGCCA [CT]^GGCC[GA]
1279 CGGCCA
EcoT14I 60 CCAAGG C^C[AT][AT]GG
955 CCATGG
FokI 273 CATCC GGATG(9/13)
780 CATCC
HaeII 1164 GGCGCC [GA]GCGC^[CT]
1618 AGCGCT
Hin1I 375 GGCGTC G[GA]^CG[CT]C
1164 GGCGCC
KspI 42 CCGCGG CCGC^GG
353 CCGCGG
NspV 183 TTCGAA TT^CGAA
585 TTCGAA
PflMI 549 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
950 CCAATCCATGG
SacI 477 GAGCTC GAGCT^C
496 GAGCTC
SacII 42 CCGCGG CCGC^GG
353 CCGCGG
SfiI 1468 GGCCCTGTAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1495 GGCCCCATAGGCC
SfuI 183 TTCGAA TT^CGAA
585 TTCGAA
SphI 578 GCATGC GCATG^C
1103 GCATGC
StyI 60 CCAAGG C^C[AT][AT]GG
955 CCATGG
Van91I 549 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
950 CCAATCCATGG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI BanI BanII BbvI BfaI
BmyI BpmI BsaJI BsgI BsiYI
BslI Bsp1286I BsrDI BsrI BssKI
BstAPI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII FauI Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I