(Circular) (Six-base) MAPSORT of: pFN21AE2033.seq Check: 3547 from: 1 to: 6577 pFN21AE2033 6577 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2682 2682 Size: 6577 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4387 278 Size: 53 83 186 936 2851 2468 Fragments arranged by size: 2851 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2384 3920 1937 Size: 447 1536 4594 Fragments arranged by size: 4594 1536 447 Acc65I G'GTAC_C Cuts at: 3898 3898 Size: 6577 AclI AA'CG_TT Cuts at: 1563 4704 5077 1563 Size: 3141 373 3063 Fragments arranged by size: 3141 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3426 4640 6024 6292 1632 Size: 1794 1214 1384 268 1917 Fragments arranged by size: 1917 1794 1384 1214 268 AfeI AGC'GCT Cuts at: 3625 3625 Size: 6577 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6380 AflIII A'CryG_T Cuts at: 1203 5476 6453 1203 Size: 4273 977 1327 Fragments arranged by size: 4273 1327 977 AgeI A'CCGG_T Cuts at: 5408 5408 Size: 6577 AhdI GACnn_n'nnGTC Cuts at: 5306 5306 Size: 6577 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6577 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6577 AlwNI CAG_nnn'CTG Cuts at: 3118 3199 3662 3703 4320 5892 3118 Size: 81 463 41 617 1572 3803 Fragments arranged by size: 3803 1572 617 463 81 41 ApaI G_GGCC'C Cuts at: 3549 3549 Size: 6577 ApaLI G'TGCA_C Cuts at: 4634 5790 4634 Size: 1156 5421 ApoI r'AATT_y Cuts at: 1493 3886 4112 4264 6373 1493 Size: 2393 226 152 2109 1697 Fragments arranged by size: 2393 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5131 160 Size: 872 4099 1606 Fragments arranged by size: 4099 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6577 AvaI C'yCGr_G Cuts at: 1870 1943 2150 3902 1870 Size: 73 207 1752 4545 Fragments arranged by size: 4545 1752 207 73 BamHI G'GATC_C Cuts at: 3907 4274 3907 Size: 367 6210 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2146 2965 3169 3898 5353 Size: 325 217 627 359 819 204 729 1455 Cuts at: 5353 618 Size: 1842 Fragments arranged by size: 1842 1455 819 729 627 359 325 217 204 BanII G_rGCy'C Cuts at: 1391 2486 2505 3549 3866 3896 1391 Size: 1095 19 1044 317 30 4072 Fragments arranged by size: 4072 1095 1044 317 30 19 BbeI G_GCGC'C Cuts at: 3173 3173 Size: 6577 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2123 928 Size: 971 224 5382 Fragments arranged by size: 5382 971 224 BbvCI CC'TCA_GC Cuts at: 3264 3264 Size: 6577 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3821 3855 4768 4802 1903 Size: 34 1884 34 913 34 3678 Fragments arranged by size: 3678 1884 913 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4469 5679 4469 Size: 1210 5367 BclI T'GATC_A Cuts at: 1548 1548 Size: 6577 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3480 3507 5188 Size: 107 122 71 631 605 1808 27 1681 Cuts at: 5188 136 Size: 1525 Fragments arranged by size: 1808 1681 1525 631 605 122 107 71 27 BglII A'GATC_T Cuts at: 6572 6572 Size: 6577 BlpI GC'TnA_GC Cuts at: 2088 3200 3994 2088 Size: 1112 794 4671 Fragments arranged by size: 4671 1112 794 Bme1580I G_kGCm'C Cuts at: 1790 2151 2968 3549 4638 5794 1790 Size: 361 817 581 1089 1156 2573 Fragments arranged by size: 2573 1156 1089 817 581 361 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6577 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3063 3479 4308 Size: 416 53 83 399 24 1646 416 829 Cuts at: 4308 5266 442 Size: 958 1753 Fragments arranged by size: 1753 1646 958 829 416 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6577 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2176 3450 2176 Size: 1274 5303 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2489 3420 3729 5237 1239 Size: 1250 931 309 1508 2579 Fragments arranged by size: 2579 1508 1250 931 309 Bpu10I CC'TnA_GC Cuts at: 2465 3264 2465 Size: 799 5778 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2500 4702 5567 5865 2500 Size: 2202 865 298 3212 Fragments arranged by size: 3212 2202 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5240 882 Size: 617 3741 2219 Fragments arranged by size: 3741 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4368 493 Size: 711 3164 2702 Fragments arranged by size: 3164 2702 711 BsaBI GATnn'nnATC Cuts at: 2549 6571 2549 Size: 4022 2555 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2381 3170 4384 4766 Size: 53 83 186 936 848 789 1214 382 Cuts at: 4766 5401 275 Size: 635 1451 Fragments arranged by size: 1451 1214 936 848 789 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5010 5408 5682 5829 6240 5010 Size: 398 274 147 411 5347 Fragments arranged by size: 5347 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6352 Fragments arranged by size: 6352 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2150 2893 1186 Size: 964 743 4870 Fragments arranged by size: 4870 964 743 BseYI C'CCAG_C Cuts at: 3144 3295 3457 3859 3867 5780 6276 3144 Size: 151 162 402 8 1913 496 3445 Fragments arranged by size: 3445 1913 496 402 162 151 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3207 3612 3716 1163 Size: 573 1471 405 104 4024 Fragments arranged by size: 4024 1471 573 405 104 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4027 4788 5816 1540 Size: 275 186 2026 761 1028 2301 Fragments arranged by size: 2301 2026 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2486 2505 4638 4723 5794 2486 Size: 19 2133 85 1071 3269 Fragments arranged by size: 3269 2133 1071 85 19 BsmI GAATG_Cn' Cuts at: 2851 4088 4181 2851 Size: 1237 93 5247 Fragments arranged by size: 5247 1237 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2151 2486 2505 2968 3549 3866 3896 Size: 399 361 335 19 463 581 317 30 Cuts at: 3896 4638 4723 5794 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 581 463 399 361 335 317 85 30 19 BspEI T'CCGG_A Cuts at: 6240 6240 Size: 6577 BspHI T'CATG_A Cuts at: 4466 6196 4466 Size: 1730 4847 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2682 3932 844 Size: 1838 1250 3489 Fragments arranged by size: 3489 1838 1250 BsrBI CCG'CTC Cuts at: 1394 1823 4464 1394 Size: 429 2641 3507 Fragments arranged by size: 3507 2641 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2359 3052 3363 3783 5072 5246 65 Size: 1183 1111 693 311 420 1289 174 1396 Fragments arranged by size: 1396 1289 1183 1111 693 420 311 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3587 5221 5408 1700 Size: 110 1777 1634 187 2869 Fragments arranged by size: 2869 1777 1634 187 110 BsrGI T'GTAC_A Cuts at: 96 2817 96 Size: 2721 3856 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6577 BssSI C'ACGA_G Cuts at: 3643 4637 5649 3643 Size: 994 1012 4571 Fragments arranged by size: 4571 1012 994 BstAPI GCAn_nnn'nTGC Cuts at: 794 3118 3313 3694 794 Size: 2324 195 381 3677 Fragments arranged by size: 3677 2324 381 195 BstBI TT'CG_AA Cuts at: 2189 2591 5381 2189 Size: 402 2790 3385 Fragments arranged by size: 3385 2790 402 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6577 BstYI r'GATC_y Cuts at: 1972 3375 3907 4274 4659 4676 6128 6214 6226 Size: 1403 532 367 385 17 1452 86 12 Cuts at: 6226 6572 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1403 532 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2328 2328 Size: 6577 BtgI C'CryG_G Cuts at: 513 1065 2004 2047 2358 2886 2960 3603 513 Size: 552 939 43 311 528 74 643 3487 Fragments arranged by size: 3487 939 643 552 528 311 74 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5838 Fragments arranged by size: 5838 683 56 BtsI GCAGTG_nn' Cuts at: 4093 4887 4907 4093 Size: 794 20 5763 Fragments arranged by size: 5763 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6577 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6542 DraI TTT'AAA Cuts at: 3881 3881 Size: 6577 DraIII CAC_nnn'GTG Cuts at: 3635 3635 Size: 6577 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5584 809 Size: 507 4268 1802 Fragments arranged by size: 4268 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2532 3284 4024 Size: 54 1425 50 275 18 702 752 740 Cuts at: 4024 4913 6542 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 752 740 702 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4024 1537 Size: 275 2212 4090 Fragments arranged by size: 4090 2212 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4507 1353 Size: 3154 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5164 5538 5684 387 Size: 1064 138 3575 374 146 1280 Fragments arranged by size: 3575 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2484 2503 2484 Size: 19 6558 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2489 3420 3426 3729 4640 5237 6024 Size: 393 857 931 6 303 911 597 787 Cuts at: 6024 6292 1239 Size: 268 1524 Fragments arranged by size: 1524 931 911 857 787 597 393 303 268 6 EcoO109I rG'GnC_Cy Cuts at: 3473 3500 3509 3536 3554 3608 3700 3811 3473 Size: 27 9 27 18 54 92 111 6239 Fragments arranged by size: 6239 111 92 54 27 27 18 9 EcoRI G'AATT_C Cuts at: 3886 4264 3886 Size: 378 6199 FspI TGC'GCA Cuts at: 5083 5083 Size: 6577 HaeII r_GCGC'y Cuts at: 1678 3173 3627 5724 1678 Size: 1495 454 2097 2531 Fragments arranged by size: 2531 2097 1495 454 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2737 2769 3193 3225 3304 Size: 32 135 32 1165 32 424 32 79 Cuts at: 3304 3317 3336 3349 3819 3851 5218 5250 5292 Size: 13 19 13 470 32 1367 32 42 Cuts at: 5292 5324 1373 Size: 32 2626 Fragments arranged by size: 2626 1367 1165 470 424 135 79 42 32 32 32 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 2385 3921 4167 669 Size: 988 281 447 1536 246 3079 Fragments arranged by size: 3079 1536 988 447 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6577 HpaI GTT'AAC Cuts at: 4167 4167 Size: 6577 KasI G'GCGC_C Cuts at: 3169 3169 Size: 6577 KpnI G_GTAC'C Cuts at: 3902 3902 Size: 6577 MfeI C'AATT_G Cuts at: 2642 4176 2642 Size: 1534 5043 MluI A'CGCG_T Cuts at: 6453 6453 Size: 6577 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5691 5875 1305 Size: 4386 184 2007 Fragments arranged by size: 4386 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2534 10 Size: 54 1425 343 702 4053 Fragments arranged by size: 4053 1425 702 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4535 4894 5053 518 Size: 648 3369 359 159 2042 Fragments arranged by size: 3369 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2028 2049 2360 3148 3247 3268 3727 3993 Size: 422 21 311 788 99 21 459 266 Cuts at: 3993 4670 5818 6063 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 788 677 459 422 311 266 245 99 21 21 NarI GG'CG_CC Cuts at: 3170 3170 Size: 6577 NcoI C'CATG_G Cuts at: 513 1065 2004 2960 513 Size: 552 939 956 4130 Fragments arranged by size: 4130 956 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6577 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6577 NotI GC'GGCC_GC Cuts at: 4024 4024 Size: 6577 NruI TCG'CGA Cuts at: 2943 2943 Size: 6577 NspI r_CATG'y Cuts at: 1132 1229 2587 2818 3112 3935 5480 1132 Size: 97 1358 231 294 823 1545 2229 Fragments arranged by size: 2229 1545 1358 823 294 231 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6577 PciI A'CATG_T Cuts at: 5476 5476 Size: 6577 PflMI CCAn_nnn'nTGG Cuts at: 1108 2560 2961 1108 Size: 1452 401 4724 Fragments arranged by size: 4724 1452 401 PmeI GTTT'AAAC Cuts at: 3881 3881 Size: 6577 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4646 4678 6159 6191 4646 Size: 32 1481 32 5032 Fragments arranged by size: 5032 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3554 3554 Size: 6577 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6577 PsiI TTA'TAA Cuts at: 4147 4147 Size: 6577 PspOMI G'GGCC_C Cuts at: 3545 3545 Size: 6577 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2356 2388 2356 Size: 32 6545 PstI C_TGCA'G Cuts at: 830 3273 3309 3571 3929 830 Size: 2443 36 262 358 3478 Fragments arranged by size: 3478 2443 358 262 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6577 PvuII CAG'CTG Cuts at: 3148 3268 3727 3148 Size: 120 459 5998 Fragments arranged by size: 5998 459 120 SacI G_AGCT'C Cuts at: 2486 2505 2486 Size: 19 6558 SacII CC_GC'GG Cuts at: 2050 2361 2050 Size: 311 6266 SalI G'TCGA_C Cuts at: 1936 2383 3919 1936 Size: 447 1536 4594 Fragments arranged by size: 4594 1536 447 SbfI CC_TGCA'GG Cuts at: 3929 3929 Size: 6577 ScaI AGT'ACT Cuts at: 4825 6537 4825 Size: 1712 4865 SfcI C'TryA_G Cuts at: 826 1045 3269 3305 3389 3468 3477 3513 3567 Size: 219 2224 36 84 79 9 36 54 Cuts at: 3567 3925 5060 5741 5932 826 Size: 358 1135 681 191 1471 Fragments arranged by size: 2224 1471 1135 681 358 219 191 84 79 54 36 36 9 SfiI GGCCn_nnn'nGGCC Cuts at: 3480 3507 3480 Size: 27 6550 SfoI GGC'GCC Cuts at: 3171 3171 Size: 6577 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6577 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6577 SmaI CCC'GGG Cuts at: 1872 3904 1872 Size: 2032 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2479 4681 5582 5844 820 Size: 197 926 536 2202 901 262 1553 Fragments arranged by size: 2202 1553 926 901 536 262 197 SnaBI TAC'GTA Cuts at: 493 4368 493 Size: 3875 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6577 SphI G_CATG'C Cuts at: 1132 2587 3112 3935 1132 Size: 1455 525 823 3774 Fragments arranged by size: 3774 1455 823 525 SspI AAT'ATT Cuts at: 5 52 4501 5 Size: 47 4449 2081 Fragments arranged by size: 4449 2081 47 StuI AGG'CCT Cuts at: 1517 2463 1517 Size: 946 5631 StyI C'CwwG_G Cuts at: 513 1065 2004 2065 2960 4016 513 Size: 552 939 61 895 1056 3074 Fragments arranged by size: 3074 1056 939 895 552 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4604 4621 4774 4959 5404 325 Size: 1255 3024 17 153 185 445 1498 Fragments arranged by size: 3024 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2817 4823 6535 Size: 274 80 33 51 1443 840 2006 1712 Cuts at: 6535 96 Size: 138 Fragments arranged by size: 2006 1712 1443 840 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6537 6569 6537 Size: 32 6545 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6577 XbaI T'CTAG_A Cuts at: 2776 3913 2776 Size: 1137 5440 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6577 XmaI C'CCGG_G Cuts at: 1870 3902 1870 Size: 2032 4545 XmnI GAAnn'nnTTC Cuts at: 2195 4706 2195 Size: 2511 4066 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4385 276 Size: 53 83 186 936 2851 2468 Fragments arranged by size: 2851 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NsiI PacI PfoI PmlI PspXI RsrII SanDI SapI SexAI SrfI SwaI XcmI