(Linear) (Six-base) MAPSORT of: KIEE2033.seq Check: 1566 from: 1 to: 1872 KIEE2033.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 677 1872 Size: 677 1195 AccI GT'mk_AC Cuts at: 0 379 1872 Size: 379 1493 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1421 1872 Size: 1421 451 AfeI AGC'GCT Cuts at: 0 1620 1872 Size: 1620 252 AlwNI CAG_nnn'CTG Cuts at: 0 1113 1194 1657 1698 1872 Size: 1113 81 463 41 174 Fragments arranged by size: 1113 463 174 81 41 ApaI G_GGCC'C Cuts at: 0 1544 1872 Size: 1544 328 AvaI C'yCGr_G Cuts at: 0 145 1872 Size: 145 1727 BanI G'GyrC_C Cuts at: 0 141 960 1164 1872 Size: 141 819 204 708 Fragments arranged by size: 819 708 204 141 BanII G_rGCy'C Cuts at: 0 481 500 1544 1861 1872 Size: 481 19 1044 317 11 Fragments arranged by size: 1044 481 317 19 11 BbeI G_GCGC'C Cuts at: 0 1168 1872 Size: 1168 704 BbsI GAAGACnn'nnnn_ Cuts at: 0 118 1872 Size: 118 1754 BbvCI CC'TCA_GC Cuts at: 0 1259 1872 Size: 1259 613 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1816 1850 1872 Size: 1816 34 22 Fragments arranged by size: 1816 34 22 BglI GCCn_nnn'nGGC Cuts at: 0 1475 1502 1872 Size: 1475 27 370 Fragments arranged by size: 1475 370 27 BlpI GC'TnA_GC Cuts at: 0 83 1195 1872 Size: 83 1112 677 Fragments arranged by size: 1112 677 83 Bme1580I G_kGCm'C Cuts at: 0 146 963 1544 1872 Size: 146 817 581 328 Fragments arranged by size: 817 581 328 146 BmrI ACTGGGnnnn_n' Cuts at: 0 1058 1474 1872 Size: 1058 416 398 Fragments arranged by size: 1058 416 398 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 171 1445 1872 Size: 171 1274 427 Fragments arranged by size: 1274 427 171 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 1415 1724 1872 Size: 484 931 309 148 Fragments arranged by size: 931 484 309 148 Bpu10I CC'TnA_GC Cuts at: 0 460 1259 1872 Size: 460 799 613 Fragments arranged by size: 799 613 460 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 495 1872 Size: 495 1377 BsaBI GATnn'nnATC Cuts at: 0 544 1872 Size: 544 1328 BsaHI Gr'CG_yC Cuts at: 0 376 1165 1872 Size: 376 789 707 Fragments arranged by size: 789 707 376 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 145 888 1872 Size: 145 743 984 Fragments arranged by size: 984 743 145 BseYI C'CCAG_C Cuts at: 0 1139 1290 1452 1854 1862 1872 Size: 1139 151 162 402 8 10 Fragments arranged by size: 1139 402 162 151 10 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1202 1607 1711 1872 Size: 1202 405 104 161 Fragments arranged by size: 1202 405 161 104 BsiHKAI G_wGCw'C Cuts at: 0 481 500 1872 Size: 481 19 1372 Fragments arranged by size: 1372 481 19 BsmI GAATG_Cn' Cuts at: 0 846 1872 Size: 846 1026 Bsp1286I G_dGCh'C Cuts at: 0 146 481 500 963 1544 1861 1872 Size: 146 335 19 463 581 317 11 Fragments arranged by size: 581 463 335 317 146 19 11 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 677 1872 Size: 677 1195 BsrDI GCAATG_nn' Cuts at: 0 354 1047 1358 1778 1872 Size: 354 693 311 420 94 Fragments arranged by size: 693 420 354 311 94 BsrFI r'CCGG_y Cuts at: 0 1582 1872 Size: 1582 290 BsrGI T'GTAC_A Cuts at: 0 812 1872 Size: 812 1060 BssSI C'ACGA_G Cuts at: 0 1638 1872 Size: 1638 234 BstAPI GCAn_nnn'nTGC Cuts at: 0 1113 1308 1689 1872 Size: 1113 195 381 183 Fragments arranged by size: 1113 381 195 183 BstBI TT'CG_AA Cuts at: 0 184 586 1872 Size: 184 402 1286 Fragments arranged by size: 1286 402 184 BstYI r'GATC_y Cuts at: 0 1370 1872 Size: 1370 502 Bsu36I CC'TnA_GG Cuts at: 0 323 1872 Size: 323 1549 BtgI C'CryG_G Cuts at: 0 42 353 881 955 1598 1872 Size: 42 311 528 74 643 274 Fragments arranged by size: 643 528 311 274 74 42 DraIII CAC_nnn'GTG Cuts at: 0 1630 1872 Size: 1630 242 EaeI y'GGCC_r Cuts at: 0 527 1279 1872 Size: 527 752 593 Fragments arranged by size: 752 593 527 EcoICRI GAG'CTC Cuts at: 0 479 498 1872 Size: 479 19 1374 Fragments arranged by size: 1374 479 19 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 1415 1421 1724 1872 Size: 484 931 6 303 148 Fragments arranged by size: 931 484 303 148 6 EcoO109I rG'GnC_Cy Cuts at: 0 1468 1495 1504 1531 1549 1603 1695 1806 Size: 1468 27 9 27 18 54 92 111 Cuts at: 1806 1872 Size: 66 Fragments arranged by size: 1468 111 92 66 54 27 27 18 9 HaeII r_GCGC'y Cuts at: 0 1168 1622 1872 Size: 1168 454 250 Fragments arranged by size: 1168 454 250 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 732 764 1188 1220 1299 1312 1331 1344 Size: 732 32 424 32 79 13 19 13 Cuts at: 1344 1814 1846 1872 Size: 470 32 26 Fragments arranged by size: 732 470 424 79 32 32 32 26 19 13 13 HincII GTy'rAC Cuts at: 0 380 1872 Size: 380 1492 KasI G'GCGC_C Cuts at: 0 1164 1872 Size: 1164 708 MfeI C'AATT_G Cuts at: 0 637 1872 Size: 637 1235 MscI TGG'CCA Cuts at: 0 529 1872 Size: 529 1343 MspA1I CmG'CkG Cuts at: 0 23 44 355 1143 1242 1263 1722 1872 Size: 23 21 311 788 99 21 459 150 Fragments arranged by size: 788 459 311 150 99 23 21 21 NarI GG'CG_CC Cuts at: 0 1165 1872 Size: 1165 707 NcoI C'CATG_G Cuts at: 0 955 1872 Size: 955 917 NruI TCG'CGA Cuts at: 0 938 1872 Size: 938 934 NspI r_CATG'y Cuts at: 0 582 813 1107 1872 Size: 582 231 294 765 Fragments arranged by size: 765 582 294 231 PflMI CCAn_nnn'nTGG Cuts at: 0 555 956 1872 Size: 555 401 916 Fragments arranged by size: 916 555 401 PpuMI rG'GwC_Cy Cuts at: 0 1549 1872 Size: 1549 323 PspOMI G'GGCC_C Cuts at: 0 1540 1872 Size: 1540 332 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 351 383 1872 Size: 351 32 1489 Fragments arranged by size: 1489 351 32 PstI C_TGCA'G Cuts at: 0 1268 1304 1566 1872 Size: 1268 36 262 306 Fragments arranged by size: 1268 306 262 36 PvuII CAG'CTG Cuts at: 0 1143 1263 1722 1872 Size: 1143 120 459 150 Fragments arranged by size: 1143 459 150 120 SacI G_AGCT'C Cuts at: 0 481 500 1872 Size: 481 19 1372 Fragments arranged by size: 1372 481 19 SacII CC_GC'GG Cuts at: 0 45 356 1872 Size: 45 311 1516 Fragments arranged by size: 1516 311 45 SalI G'TCGA_C Cuts at: 0 378 1872 Size: 378 1494 SfcI C'TryA_G Cuts at: 0 1264 1300 1384 1463 1472 1508 1562 1872 Size: 1264 36 84 79 9 36 54 310 Fragments arranged by size: 1264 310 84 79 54 36 36 9 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1475 1502 1872 Size: 1475 27 370 Fragments arranged by size: 1475 370 27 SfoI GGC'GCC Cuts at: 0 1166 1872 Size: 1166 706 SmlI C'TyrA_G Cuts at: 0 474 1872 Size: 474 1398 SphI G_CATG'C Cuts at: 0 582 1107 1872 Size: 582 525 765 Fragments arranged by size: 765 582 525 StuI AGG'CCT Cuts at: 0 458 1872 Size: 458 1414 StyI C'CwwG_G Cuts at: 0 60 955 1872 Size: 60 895 917 Fragments arranged by size: 917 895 60 TatI w'GTAC_w Cuts at: 0 812 1872 Size: 812 1060 XbaI T'CTAG_A Cuts at: 0 771 1872 Size: 771 1101 XmnI GAAnn'nnTTC Cuts at: 0 190 1872 Size: 190 1682 Enzymes that do cut: AarI AccI AcuI AfeI AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI DraIII EaeI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KasI MfeI MscI MspA1I NarI NcoI NruI NspI PflMI PpuMI PspOMI PsrI PstI PvuII SacI SacII SalI SfcI SfiI SfoI SmlI SphI StuI StyI TatI XbaI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BglII BmgBI BmtI BsaI BsaAI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BssHII BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MluI MmeI MslI NaeI NdeI NgoMIV NheI NotI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TaqII TstI Tth111I XcmI XhoI XmaI ZraI