Restriction map for
pFN21AE2746
(Circular) (Six-base) MAPSORT of: pFN21AE2746.seq Check: 8413 from: 1 to: 6466
pFN21AE2746 6466 bp
With 182 enzymes: SgfI *
April 23, 2019 15:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4276 278
Size: 53 83 186 936 2740 2468
Fragments arranged by size:
2740 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3809 1937
Size: 1872 4594
Acc65I G'GTAC_C
Cuts at: 3787 3787
Size: 6466
AclI AA'CG_TT
Cuts at: 1563 3718 4593 4966 1563
Size: 2155 875 373 3063
Fragments arranged by size:
3063 2155 875 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2416 2893 4529 5913 6181 1632
Size: 784 477 1636 1384 268 1917
Fragments arranged by size:
1917 1636 1384 784 477 268
AflII C'TTAA_G
Cuts at: 820 1017 2162 820
Size: 197 1145 5124
Fragments arranged by size:
5124 1145 197
AflIII A'CryG_T
Cuts at: 1203 5365 6342 1203
Size: 4162 977 1327
Fragments arranged by size:
4162 1327 977
AgeI A'CCGG_T
Cuts at: 5297 5297
Size: 6466
AhdI GACnn_n'nnGTC
Cuts at: 5195 5195
Size: 6466
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6466
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2273 3521 1184
Size: 1089 1248 4129
Fragments arranged by size:
4129 1248 1089
AlwNI CAG_nnn'CTG
Cuts at: 2098 2731 4209 5781 2098
Size: 633 1478 1572 2783
Fragments arranged by size:
2783 1572 1478 633
ApaI G_GGCC'C
Cuts at: 2057 3061 3744 2057
Size: 1004 683 4779
Fragments arranged by size:
4779 1004 683
ApaLI G'TGCA_C
Cuts at: 4523 5679 4523
Size: 1156 5310
ApoI r'AATT_y
Cuts at: 1493 3775 4001 4153 6262 1493
Size: 2282 226 152 2109 1697
Fragments arranged by size:
2282 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5020 160
Size: 872 3988 1606
Fragments arranged by size:
3988 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
AvaI C'yCGr_G
Cuts at: 1870 1943 2049 3023 3105 3534 3791 1870
Size: 73 106 974 82 429 257 4545
Fragments arranged by size:
4545 974 429 257 106 82 73
AvrII C'CTAG_G
Cuts at: 2068 2068
Size: 6466
BamHI G'GATC_C
Cuts at: 3796 4163 3796
Size: 367 6099
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2678 2696 3008 3559 3787
Size: 325 217 627 891 18 312 551 228
Cuts at: 3787 5242 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 891 627 551 325 312 228
217 18
BanII G_rGCy'C
Cuts at: 1391 2057 3061 3298 3352 3549 3702 3744 3785
Size: 666 1004 237 54 197 153 42 41
Cuts at: 3785 1391
Size: 4072
Fragments arranged by size:
4072 1004 666 237 197 153 54 42
41
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2422 2899 928
Size: 971 523 477 4495
Fragments arranged by size:
4495 971 523 477
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4657 4691 1903
Size: 34 2720 34 3678
Fragments arranged by size:
3678 2720 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2888 4358 5568 2888
Size: 1470 1210 3786
Fragments arranged by size:
3786 1470 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6466
BfrBI ATG'CAT
Cuts at: 2525 3142 2525
Size: 617 5849
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2070 5077 136
Size: 107 122 71 631 605 398 3007 1525
Fragments arranged by size:
3007 1525 631 605 398 122 107 71
BglII A'GATC_T
Cuts at: 2308 2376 6461 2308
Size: 68 4085 2313
Fragments arranged by size:
4085 2313 68
BlpI GC'TnA_GC
Cuts at: 2736 3883 2736
Size: 1147 5319
Bme1580I G_kGCm'C
Cuts at: 1790 2057 3061 3744 4527 5683 1790
Size: 267 1004 683 783 1156 2573
Fragments arranged by size:
2573 1156 1004 783 683 267
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6466
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2033 3739 4197
Size: 416 53 83 399 24 616 1706 458
Cuts at: 4197 5155 442
Size: 958 1753
Fragments arranged by size:
1753 1706 958 616 458 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6466
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2395 2857 2395
Size: 462 6004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2199 2219 2688 3312 3629 5126 1239
Size: 960 20 469 624 317 1497 2579
Fragments arranged by size:
2579 1497 960 624 469 317 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2741 3302 4591 5456 5754 2741
Size: 561 1289 865 298 3453
Fragments arranged by size:
3453 1289 865 561 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2332 2639 5129 882
Size: 617 833 307 2490 2219
Fragments arranged by size:
2490 2219 833 617 307
BsaAI yAC'GTr
Cuts at: 493 1204 4257 493
Size: 711 3053 2702
Fragments arranged by size:
3053 2702 711
BsaBI GATnn'nnATC
Cuts at: 2526 3152 6460 2526
Size: 626 3308 2532
Fragments arranged by size:
3308 2532 626
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4273 4655 5290 275
Size: 53 83 186 936 2740 382 635 1451
Fragments arranged by size:
2740 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2354 4899 5297 5571 5718 6129 2354
Size: 2545 398 274 147 411 2691
Fragments arranged by size:
2691 2545 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6241
Fragments arranged by size:
6241 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2196 2761 3041 3341 1186
Size: 1010 565 280 300 4311
Fragments arranged by size:
4311 1010 565 300 280
BseYI C'CCAG_C
Cuts at: 2514 3113 3419 5669 6165 2514
Size: 599 306 2250 496 2815
Fragments arranged by size:
2815 2250 599 496 306
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2279 2726 3527 1163
Size: 573 543 447 801 4102
Fragments arranged by size:
4102 801 573 543 447
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3916 4677 5705 1540
Size: 275 186 1915 761 1028 2301
Fragments arranged by size:
2301 1915 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3288 3298 3549 3702 4527 4612 5683 3288
Size: 10 251 153 825 85 1071 4071
Fragments arranged by size:
4071 1071 825 251 153 85 10
BsmI GAATG_Cn'
Cuts at: 3977 4070 3977
Size: 93 6373
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 3061 3288 3298 3352 3549 3702
Size: 399 267 1004 227 10 54 197 153
Cuts at: 3702 3744 3785 4527 4612 5683 1391
Size: 42 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 1004 742 399 267 227 197
153 85 54 42 41 10
BspEI T'CCGG_A
Cuts at: 6129 6129
Size: 6466
BspHI T'CATG_A
Cuts at: 3702 4355 6085 3702
Size: 653 1730 4083
Fragments arranged by size:
4083 1730 653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3821 844
Size: 2977 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4353 1394
Size: 429 2530 3507
Fragments arranged by size:
3507 2530 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3201 3367 3454 4961 5135 65
Size: 1183 1953 166 87 1507 174 1396
Fragments arranged by size:
1953 1507 1396 1183 174 166 87
BsrFI r'CCGG_y
Cuts at: 1700 1810 5110 5297 1700
Size: 110 3300 187 2869
Fragments arranged by size:
3300 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3688 96
Size: 3592 2874
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6466
BssSI C'ACGA_G
Cuts at: 4526 5538 4526
Size: 1012 5454
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6466
BstBI TT'CG_AA
Cuts at: 5270 5270
Size: 6466
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6466
BstXI CCAn_nnnn'nTGG
Cuts at: 2977 3053 3442 2977
Size: 76 389 6001
Fragments arranged by size:
6001 389 76
BstYI r'GATC_y
Cuts at: 1972 2308 2376 2817 3796 4163 4548 4565 6017
Size: 336 68 441 979 367 385 17 1452
Cuts at: 6017 6103 6115 6461 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 979 441 385 367 346 336
86 68 17 12
Bsu36I CC'TnA_GG
Cuts at: 2339 2622 3341 2339
Size: 283 719 5464
Fragments arranged by size:
5464 719 283
BtgI C'CryG_G
Cuts at: 513 1065 2004 3000 3279 513
Size: 552 939 996 279 3700
Fragments arranged by size:
3700 996 939 552 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5727
Fragments arranged by size:
5727 683 56
BtsI GCAGTG_nn'
Cuts at: 3982 4776 4796 3982
Size: 794 20 5652
Fragments arranged by size:
5652 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6466
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6431
DraI TTT'AAA
Cuts at: 3770 3770
Size: 6466
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5473 809
Size: 507 4157 1802
Fragments arranged by size:
4157 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2811 3913 4802
Size: 54 1425 50 275 18 981 1102 889
Cuts at: 4802 6431 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1102 981 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3913 1537
Size: 275 2101 4090
Fragments arranged by size:
4090 2101 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2240 2771 2807 3537 4396 1353
Size: 887 531 36 730 859 3423
Fragments arranged by size:
3423 887 859 730 531 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5053 5427 5573 387
Size: 1064 138 3464 374 146 1280
Fragments arranged by size:
3464 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3296 3547 3700 3296
Size: 251 153 6062
Fragments arranged by size:
6062 251 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 2219 2416 2688 2893 3312 3629
Size: 393 567 20 197 272 205 419 317
Cuts at: 3629 4529 5126 5913 6181 1239
Size: 900 597 787 268 1524
Fragments arranged by size:
1524 900 787 597 567 419 393 317
272 268 205 197 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2072 2072
Size: 6466
EcoO109I rG'GnC_Cy
Cuts at: 2053 2054 3057 3058 3102 3397 3740 3741 2053
Size: 1 1003 1 44 295 343 1 4778
Fragments arranged by size:
4778 1003 343 295 44 1 1 1
EcoRI G'AATT_C
Cuts at: 3775 4153 3775
Size: 378 6088
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2923 2923
Size: 6466
FspI TGC'GCA
Cuts at: 4972 4972
Size: 6466
HaeII r_GCGC'y
Cuts at: 1678 3114 5613 1678
Size: 1436 2499 2531
Fragments arranged by size:
2531 2499 1436
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2209 3143 3175 5107
Size: 32 135 32 605 32 934 32 1932
Cuts at: 5107 5139 5181 5213 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1932 934 605 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3810 4056 669
Size: 988 281 1872 246 3079
Fragments arranged by size:
3079 1872 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6466
HpaI GTT'AAC
Cuts at: 4056 4056
Size: 6466
KpnI G_GTAC'C
Cuts at: 3791 3791
Size: 6466
MfeI C'AATT_G
Cuts at: 4065 4065
Size: 6466
MluI A'CGCG_T
Cuts at: 6342 6342
Size: 6466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2208 5580 5764 1305
Size: 903 3372 184 2007
Fragments arranged by size:
3372 2007 903 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2813 10
Size: 54 1425 343 981 3663
Fragments arranged by size:
3663 1425 981 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3051 3440 3503 4424 4783 4942 518
Size: 648 1885 389 63 921 359 159 2042
Fragments arranged by size:
2042 1885 921 648 389 359 159 63
MspA1I CmG'CkG
Cuts at: 1606 2514 3882 4559 5707 5952 1606
Size: 908 1368 677 1148 245 2120
Fragments arranged by size:
2120 1368 1148 908 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3000 3279 513
Size: 552 939 996 279 3700
Fragments arranged by size:
3700 996 939 552 279
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6466
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6466
NotI GC'GGCC_GC
Cuts at: 3913 3913
Size: 6466
NsiI A_TGCA'T
Cuts at: 2527 3144 2527
Size: 617 5849
NspI r_CATG'y
Cuts at: 1132 1229 3376 3824 5369 1132
Size: 97 2147 448 1545 2229
Fragments arranged by size:
2229 2147 1545 448 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6466
PciI A'CATG_T
Cuts at: 5365 5365
Size: 6466
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3001 1108
Size: 1893 4573
PfoI T'CCnGG_A
Cuts at: 2235 2280 3645 2235
Size: 45 1365 5056
Fragments arranged by size:
5056 1365 45
PmeI GTTT'AAAC
Cuts at: 3770 3770
Size: 6466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4535 4567 6048 6080 4535
Size: 32 1481 32 4921
Fragments arranged by size:
4921 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3102 3102
Size: 6466
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6466
PsiI TTA'TAA
Cuts at: 4036 4036
Size: 6466
PspOMI G'GGCC_C
Cuts at: 2053 3057 3740 2053
Size: 1004 683 4779
Fragments arranged by size:
4779 1004 683
PspXI vC'TCGA_Gb
Cuts at: 3023 3023
Size: 6466
PstI C_TGCA'G
Cuts at: 830 2017 2655 3250 3818 830
Size: 1187 638 595 568 3478
Fragments arranged by size:
3478 1187 638 595 568
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6466
SacI G_AGCT'C
Cuts at: 3298 3549 3702 3298
Size: 251 153 6062
Fragments arranged by size:
6062 251 153
SalI G'TCGA_C
Cuts at: 1936 3808 1936
Size: 1872 4594
SanDI GG'GwC_CC
Cuts at: 3102 3102
Size: 6466
SapI GCTCTTCn'nnn_
Cuts at: 2240 2771 3537 2240
Size: 531 766 5169
Fragments arranged by size:
5169 766 531
SbfI CC_TGCA'GG
Cuts at: 3818 3818
Size: 6466
ScaI AGT'ACT
Cuts at: 4714 6426 4714
Size: 1712 4754
SfcI C'TryA_G
Cuts at: 826 1045 2013 2027 2651 3087 3246 3814 4949
Size: 219 968 14 624 436 159 568 1135
Cuts at: 4949 5630 5821 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 968 681 624 568 436 219
191 159 14
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6466
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6466
SmaI CCC'GGG
Cuts at: 1872 2051 3107 3536 3793 1872
Size: 179 1056 429 257 4545
Fragments arranged by size:
4545 1056 429 257 179
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2162 2720 3023 3317 4570 5471
Size: 197 926 219 558 303 294 1253 901
Cuts at: 5471 5733 820
Size: 262 1553
Fragments arranged by size:
1553 1253 926 901 558 303 294 262
219 197
SnaBI TAC'GTA
Cuts at: 493 4257 493
Size: 3764 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6466
SphI G_CATG'C
Cuts at: 1132 3824 1132
Size: 2692 3774
SspI AAT'ATT
Cuts at: 5 52 4390 5
Size: 47 4338 2081
Fragments arranged by size:
4338 2081 47
StuI AGG'CCT
Cuts at: 1517 3179 1517
Size: 1662 4804
StyI C'CwwG_G
Cuts at: 513 1065 2004 2068 2134 2808 2872 3000 3279
Size: 552 939 64 66 674 64 128 279
Cuts at: 3279 3905 513
Size: 626 3074
Fragments arranged by size:
3074 939 674 626 552 279 128 66
64 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4493 4510 4663 4848 5293 325
Size: 1255 2913 17 153 185 445 1498
Fragments arranged by size:
2913 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3688 4712 6424
Size: 274 80 33 51 1443 1711 1024 1712
Cuts at: 6424 96
Size: 138
Fragments arranged by size:
1712 1711 1443 1024 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6426 6458 6426
Size: 32 6434
Tth111I GACn'n_nGTC
Cuts at: 1316 2115 1316
Size: 799 5667
XbaI T'CTAG_A
Cuts at: 2944 3298 3802 2944
Size: 354 504 5608
Fragments arranged by size:
5608 504 354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2099 3002 2099
Size: 903 5563
XhoI C'TCGA_G
Cuts at: 1943 3023 1943
Size: 1080 5386
XmaI C'CCGG_G
Cuts at: 1870 2049 3105 3534 3791 1870
Size: 179 1056 429 257 4545
Fragments arranged by size:
4545 1056 429 257 179
XmnI GAAnn'nnTTC
Cuts at: 2297 4595 2297
Size: 2298 4168
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4274 276
Size: 53 83 186 936 2740 2468
Fragments arranged by size:
2740 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI SacI SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PsrI PvuII
RsrII SacII SexAI SfiI SfoI SrfI SwaI