Restriction map for
pFN21AE2746
(Linear) (Six-base) MAPSORT of: KIEE2746.seq Check: 2766 from: 1 to: 1761
KIEE2746.orf
With 182 enzymes: SgfI *
April 23, 2019 13:01 ..
AclI AA'CG_TT
Cuts at: 0 1713 1761
Size: 1713 48
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 888 1761
Size: 411 477 873
Fragments arranged by size:
873 477 411
AflII C'TTAA_G
Cuts at: 0 157 1761
Size: 157 1604
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 268 1516 1761
Size: 268 1248 245
Fragments arranged by size:
1248 268 245
AlwNI CAG_nnn'CTG
Cuts at: 0 93 726 1761
Size: 93 633 1035
Fragments arranged by size:
1035 633 93
ApaI G_GGCC'C
Cuts at: 0 52 1056 1739 1761
Size: 52 1004 683 22
Fragments arranged by size:
1004 683 52 22
AvaI C'yCGr_G
Cuts at: 0 44 1018 1100 1529 1761
Size: 44 974 82 429 232
Fragments arranged by size:
974 429 232 82 44
AvrII C'CTAG_G
Cuts at: 0 63 1761
Size: 63 1698
BanI G'GyrC_C
Cuts at: 0 673 691 1003 1554 1761
Size: 673 18 312 551 207
Fragments arranged by size:
673 551 312 207 18
BanII G_rGCy'C
Cuts at: 0 52 1056 1293 1347 1544 1697 1739 1761
Size: 52 1004 237 54 197 153 42 22
Fragments arranged by size:
1004 237 197 153 54 52 42 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 417 894 1761
Size: 417 477 867
Fragments arranged by size:
867 477 417
BciVI GTATCCnnnnn_n'
Cuts at: 0 883 1761
Size: 883 878
BfrBI ATG'CAT
Cuts at: 0 520 1137 1761
Size: 520 617 624
Fragments arranged by size:
624 617 520
BglI GCCn_nnn'nGGC
Cuts at: 0 65 1761
Size: 65 1696
BglII A'GATC_T
Cuts at: 0 303 371 1761
Size: 303 68 1390
Fragments arranged by size:
1390 303 68
BlpI GC'TnA_GC
Cuts at: 0 731 1761
Size: 731 1030
Bme1580I G_kGCm'C
Cuts at: 0 52 1056 1739 1761
Size: 52 1004 683 22
Fragments arranged by size:
1004 683 52 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 28 1734 1761
Size: 28 1706 27
Fragments arranged by size:
1706 28 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 390 852 1761
Size: 390 462 909
Fragments arranged by size:
909 462 390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 214 683 1307 1624 1761
Size: 194 20 469 624 317 137
Fragments arranged by size:
624 469 317 194 137 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 1297 1761
Size: 736 561 464
Fragments arranged by size:
736 561 464
BsaI GGTCTCn'nnnn_
Cuts at: 0 327 634 1761
Size: 327 307 1127
Fragments arranged by size:
1127 327 307
BsaBI GATnn'nnATC
Cuts at: 0 521 1147 1761
Size: 521 626 614
Fragments arranged by size:
626 614 521
BsaWI w'CCGG_w
Cuts at: 0 349 1761
Size: 349 1412
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 191 756 1036 1336 1761
Size: 191 565 280 300 425
Fragments arranged by size:
565 425 300 280 191
BseYI C'CCAG_C
Cuts at: 0 509 1108 1414 1761
Size: 509 599 306 347
Fragments arranged by size:
599 509 347 306
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 721 1522 1761
Size: 274 447 801 239
Fragments arranged by size:
801 447 274 239
BsiHKAI G_wGCw'C
Cuts at: 0 1283 1293 1544 1697 1761
Size: 1283 10 251 153 64
Fragments arranged by size:
1283 251 153 64 10
Bsp1286I G_dGCh'C
Cuts at: 0 52 1056 1283 1293 1347 1544 1697 1739
Size: 52 1004 227 10 54 197 153 42
Cuts at: 1739 1761
Size: 22
Fragments arranged by size:
1004 227 197 153 54 52 42 22
10
BspHI T'CATG_A
Cuts at: 0 1697 1761
Size: 1697 64
BsrDI GCAATG_nn'
Cuts at: 0 1196 1362 1449 1761
Size: 1196 166 87 312
Fragments arranged by size:
1196 312 166 87
BsrGI T'GTAC_A
Cuts at: 0 1683 1761
Size: 1683 78
BstXI CCAn_nnnn'nTGG
Cuts at: 0 972 1048 1437 1761
Size: 972 76 389 324
Fragments arranged by size:
972 389 324 76
BstYI r'GATC_y
Cuts at: 0 303 371 812 1761
Size: 303 68 441 949
Fragments arranged by size:
949 441 303 68
Bsu36I CC'TnA_GG
Cuts at: 0 334 617 1336 1761
Size: 334 283 719 425
Fragments arranged by size:
719 425 334 283
BtgI C'CryG_G
Cuts at: 0 995 1274 1761
Size: 995 279 487
Fragments arranged by size:
995 487 279
EaeI y'GGCC_r
Cuts at: 0 806 1761
Size: 806 955
EarI CTCTTCn'nnn_
Cuts at: 0 235 766 802 1532 1761
Size: 235 531 36 730 229
Fragments arranged by size:
730 531 235 229 36
EcoICRI GAG'CTC
Cuts at: 0 1291 1542 1695 1761
Size: 1291 251 153 66
Fragments arranged by size:
1291 251 153 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 214 411 683 888 1307 1624 1761
Size: 194 20 197 272 205 419 317 137
Fragments arranged by size:
419 317 272 205 197 194 137 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 67 1761
Size: 67 1694
EcoO109I rG'GnC_Cy
Cuts at: 0 48 49 1052 1053 1097 1392 1735 1736
Size: 48 1 1003 1 44 295 343 1
Cuts at: 1736 1761
Size: 25
Fragments arranged by size:
1003 343 295 48 44 25 1 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 918 1761
Size: 918 843
HaeII r_GCGC'y
Cuts at: 0 1109 1761
Size: 1109 652
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 1138 1170 1761
Size: 172 32 934 32 591
Fragments arranged by size:
934 591 172 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 1761
Size: 203 1558
MscI TGG'CCA
Cuts at: 0 808 1761
Size: 808 953
MslI CAynn'nnrTG
Cuts at: 0 1046 1435 1498 1761
Size: 1046 389 63 263
Fragments arranged by size:
1046 389 263 63
MspA1I CmG'CkG
Cuts at: 0 509 1761
Size: 509 1252
NcoI C'CATG_G
Cuts at: 0 995 1274 1761
Size: 995 279 487
Fragments arranged by size:
995 487 279
NsiI A_TGCA'T
Cuts at: 0 522 1139 1761
Size: 522 617 622
Fragments arranged by size:
622 617 522
NspI r_CATG'y
Cuts at: 0 1371 1761
Size: 1371 390
PflMI CCAn_nnn'nTGG
Cuts at: 0 996 1761
Size: 996 765
PfoI T'CCnGG_A
Cuts at: 0 230 275 1640 1761
Size: 230 45 1365 121
Fragments arranged by size:
1365 230 121 45
PpuMI rG'GwC_Cy
Cuts at: 0 1097 1761
Size: 1097 664
PspOMI G'GGCC_C
Cuts at: 0 48 1052 1735 1761
Size: 48 1004 683 26
Fragments arranged by size:
1004 683 48 26
PspXI vC'TCGA_Gb
Cuts at: 0 1018 1761
Size: 1018 743
PstI C_TGCA'G
Cuts at: 0 12 650 1245 1761
Size: 12 638 595 516
Fragments arranged by size:
638 595 516 12
SacI G_AGCT'C
Cuts at: 0 1293 1544 1697 1761
Size: 1293 251 153 64
Fragments arranged by size:
1293 251 153 64
SanDI GG'GwC_CC
Cuts at: 0 1097 1761
Size: 1097 664
SapI GCTCTTCn'nnn_
Cuts at: 0 235 766 1532 1761
Size: 235 531 766 229
Fragments arranged by size:
766 531 235 229
SfcI C'TryA_G
Cuts at: 0 8 22 646 1082 1241 1761
Size: 8 14 624 436 159 520
Fragments arranged by size:
624 520 436 159 14 8
SmaI CCC'GGG
Cuts at: 0 46 1102 1531 1761
Size: 46 1056 429 230
Fragments arranged by size:
1056 429 230 46
SmlI C'TyrA_G
Cuts at: 0 157 715 1018 1312 1761
Size: 157 558 303 294 449
Fragments arranged by size:
558 449 303 294 157
StuI AGG'CCT
Cuts at: 0 1174 1761
Size: 1174 587
StyI C'CwwG_G
Cuts at: 0 63 129 803 867 995 1274 1761
Size: 63 66 674 64 128 279 487
Fragments arranged by size:
674 487 279 128 66 64 63
TatI w'GTAC_w
Cuts at: 0 1683 1761
Size: 1683 78
Tth111I GACn'n_nGTC
Cuts at: 0 110 1761
Size: 110 1651
XbaI T'CTAG_A
Cuts at: 0 939 1293 1761
Size: 939 354 468
Fragments arranged by size:
939 468 354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 94 997 1761
Size: 94 903 764
Fragments arranged by size:
903 764 94
XhoI C'TCGA_G
Cuts at: 0 1018 1761
Size: 1018 743
XmaI C'CCGG_G
Cuts at: 0 44 1100 1529 1761
Size: 44 1056 429 232
Fragments arranged by size:
1056 429 232 44
XmnI GAAnn'nnTTC
Cuts at: 0 292 1761
Size: 292 1469
Enzymes that do cut:
AclI AcuI AflII AlfI AlwNI ApaI AvaI AvrII
BanI BanII BbsI BciVI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaBI BsaWI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BsrDI BsrGI
BstXI BstYI Bsu36I BtgI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I MmeI MscI MslI
MspA1I NcoI NsiI NspI PflMI PfoI PpuMI PspOMI
PspXI PstI SacI SanDI SapI SfcI SmaI SmlI
StuI StyI TatI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflIII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI BaeI
BamHI BbeI BbvCI BcgI BclI BmgBI BmtI Bpu10I
BsaAI BsaHI BsaXI BsiEI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PsrI PvuI PvuII
RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TstI ZraI